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Gene information
General information
TC004512 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3587648..3594437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004512
    iBeetle screen iB_06179

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004512 RA
      MAGILSLVW FIGSVLVTP FLVILLCIV FLASIGKSL 
      GVRRLYVKL LLIIFEYGR ANIETAQNQ AHRNEDSDD 
      EITAPNNNT AKDQLIKPP NGIKKPNNG YVPNDNSVI 
      SRDDKLILL PEPLQKKDE ITGEITESK DEYKEYNLS 
      SIFDYLKAG MEAIIEDQV TSRFEAEEL KNWNLLIRT 
      NRRYEFISW KLTVIWVCG FFVRYFFLF PLRVTICFF 
      GVWWLTLCT ALVGYVKEG SFKQKLNKY VSIMCFGIL 
      SNAISSVIT YHNEENKPK NGICVANHT SPIDVLILM 
      CDNCYSLIG QSHGGFLGI LQRALARAS PHIWFERSE 
      VRDRHAVAN KLKEHVSNP KNPPILIFP EGTCINNTS 
      VMQFKKGSF EVGSVIYPV AIKYDPRFG DAFWNSSKY 
      SMMQYLYMM MTSWAIVCD VWYLPPMQQ EEGESAIDF 
      ANRVKSVIA KQGGLVDLV WDGQLKRTK PKKEWRERQ 
      QEEFSKRLK IE
    CDS sequence
    • >TC004512 RA
      atggctggt attttatcc ctggtttgg tttatcggt 
      tccgtttta gtaacaccg tttttggtg attttatta 
      tgtattgta tttcttgca tcaattgga aaatcatta 
      ggcgttagg agactttat gttaaactt cttttaatc 
      atatttgag tacggtcgt gcaaacatt gaaaccgct 
      caaaatcaa gcacacaga aacgaagac agcgacgat 
      gaaatcact gcccccaac aacaacacc gccaaagac 
      caactcatc aaaccgcca aacggaatc aaaaaaccc 
      aacaatggc tacgtgcca aacgataac tcggttata 
      agcagggat gataaactg atcttgttg ccggaacct 
      ttacagaaa aaggacgaa ataactggc gaaataacc 
      gaatctaag gacgaatac aaggaatat aatttgagc 
      agtattttt gattatttg aaggccggc atggaagcc 
      atcatcgag gatcaagtc acgtcgagg tttgaagct 
      gaagaactg aagaattgg aatttattg attcgaacg 
      aatcggaga tacgaattt atttcgtgg aaacttacc 
      gtgatatgg gtgtgcgga tttttcgtc agatatttc 
      ttcctgttt ccgttgcgt gtcaccatt tgctttttt 
      ggggtgtgg tggttgacg ctgtgtacg gccttagtt 
      gggtatgtg aaggagggc agttttaaa caaaaatta 
      aacaagtac gtgtcaatt atgtgcttt ggaattttg 
      tccaatgcg atatcgtca gtcattacg tatcataac 
      gaagagaat aagccgaaa aatgggatt tgtgtcgcc 
      aatcatacg tcgccgatt gatgtactt atattgatg 
      tgtgataat tgttattct ttgattggt caaagtcac 
      ggcggtttt ttgggcatt ttgcagcga gctttagct 
      cgcgcctct ccacatatc tggtttgaa agatccgaa 
      gttagggat cgccacgca gttgccaac aagttgaaa 
      gagcacgtg tccaatccg aaaaacccc ccgattttg 
      atattcccc gagggcact tgtatcaac aacacgtcg 
      gtaatgcag ttcaagaaa ggtagtttt gaggttggc 
      agtgttatc tatccagtg gctataaaa tacgatcca 
      cgattcggc gatgctttt tggaacagt agtaaatac 
      tcgatgatg caatatttg tacatgatg atgaccagt 
      tgggcgatt gtttgtgat gtctggtat ttaccccca 
      atgcagcaa gaagagggc gaaagtgct atagatttt 
      gcaaatcga gtcaaaagt gtgatagct aagcagggc 
      ggtttggtc gatctagtc tgggacggc caactcaaa 
      cgcacaaaa ccaaagaaa gaatggcgc gaacggcaa 
      caagaagag ttcagtaaa cgacttaaa atagaatag
    mRNA sequence
    • >TC004512 RA
      tatttagtg ataccatgt gcgtgttgt gtcgttaat 
      gtgattttg tcaatttaa atttcgctc ttacaatgg 
      ctggtattt tatccctgg tttggttta tcggttccg 
      ttttagtaa caccgtttt tggtgattt tattatgta 
      ttgtatttc ttgcatcaa ttggaaaat cattaggcg 
      ttaggagac tttatgtta aacttcttt taatcatat 
      ttgagtacg gtcgtgcaa acattgaaa ccgctcaaa 
      atcaagcac acagaaacg aagacagcg acgatgaaa 
      tcactgccc ccaacaaca acaccgcca aagaccaac 
      tcatcaaac cgccaaacg gaatcaaaa aacccaaca 
      atggctacg tgccaaacg ataactcgg ttataagca 
      gggatgata aactgatct tgttgccgg aacctttac 
      agaaaaagg acgaaataa ctggcgaaa taaccgaat 
      ctaaggacg aatacaagg aatataatt tgagcagta 
      tttttgatt atttgaagg ccggcatgg aagccatca 
      tcgaggatc aagtcacgt cgaggtttg aagctgaag 
      aactgaaga attggaatt tattgattc gaacgaatc 
      ggagatacg aatttattt cgtggaaac ttaccgtga 
      tatgggtgt gcggatttt tcgtcagat atttcttcc 
      tgtttccgt tgcgtgtca ccatttgct tttttgggg 
      tgtggtggt tgacgctgt gtacggcct tagttgggt 
      atgtgaagg agggcagtt ttaaacaaa aattaaaca 
      agtacgtgt caattatgt gctttggaa ttttgtcca 
      atgcgatat cgtcagtca ttacgtatc ataacgaag 
      agaataagc cgaaaaatg ggatttgtg tcgccaatc 
      atacgtcgc cgattgatg tacttatat tgatgtgtg 
      ataattgtt attctttga ttggtcaaa gtcacggcg 
      gttttttgg gcattttgc agcgagctt tagctcgcg 
      cctctccac atatctggt ttgaaagat ccgaagtta 
      gggatcgcc acgcagttg ccaacaagt tgaaagagc 
      acgtgtcca atccgaaaa accccccga ttttgatat 
      tccccgagg gcacttgta tcaacaaca cgtcggtaa 
      tgcagttca agaaaggta gttttgagg ttggcagtg 
      ttatctatc cagtggcta taaaatacg atccacgat 
      tcggcgatg ctttttgga acagtagta aatactcga 
      tgatgcaat atttgtaca tgatgatga ccagttggg 
      cgattgttt gtgatgtct ggtatttac ccccaatgc 
      agcaagaag agggcgaaa gtgctatag attttgcaa 
      atcgagtca aaagtgtga tagctaagc agggcggtt 
      tggtcgatc tagtctggg acggccaac tcaaacgca 
      caaaaccaa agaaagaat ggcgcgaac ggcaacaag 
      aagagttca gtaaacgac ttaaaatag aatagaccc 
      ttgcagtat cttatttta attctgtta ttttcattt 
      ttttacaca tctagtctc ataattttt caatttgtt 
      tcatcgtga tagcgtaag tagagtaat ttggagcac 
      attttttgg cctaaatgt tatttgttt ggtggcgcc 
      cgtaatggt ccgatttga tgcttaagt cttcaagtt 
      gctcacatc agtgtcaaa attcatgta agaaatcga 
      caatataat g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY