Status
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Gene information
General information
TC034856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 715644..725277
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034856
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06173

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034856 RA
      MFRNELQDD GDLRTEIFH LKKNLSNIS TENRMLKVK 
      IRKLQNEII KKDKQIDAI LDPKKNKYS DDLNWDNEI 
      GQIQIKVPT SKNVKHTIF NDEIKKKTN RSLSWTCEA 
      ESSVEFNKK HQNERSKSG GKDQYKNFL STIKDSNSR 
      QTSSRKSSP ESDLERIEL LCESPHEEI QQLHSYFLD 
      IIAELGHLK NTVTELQID KNKTDSAIK KENFTIDML 
      AKELKNLRL NKPKPNRNY SRRFHSADL VAKVYTANK 
      VTNKSVKEN KSKDKTKGK KTGSEGTNG PKFKFKNNK 
      TQGTKSVMV QVDDLNDNT EFSNQCLYI ERDNITIKE 
      KSKSPDDRE DEVLTLMLT TSVWFFCII MHIIMSTRS 
      HLDLHVSED VAYIVVFCG LYYITFNYV KNQHKIALL 
      LRDLSNFDK FGVPPGYEN VETKLGFYS KFLFAYVTV 
      AGVVYNFMR FIEKGECEN MTGKDFNEV CGLMSPLWI 
      PISLNNNSL VYCAIFLYV LICSQMVIK VGLMISYHA 
      TEMAHHIIL RIDHLKLMI VKCFDLEYN ELQRRKLRQ 
      CILYHTEIL DFASRLDEC FSTGMFTHI FMTGAIFAC 
      IEKQFIDGE HQFCAAVHF IGWVLTLFV ACLGGQHLI 
      NAWYKADKE LRKDLLFML MRCQKSLKI RAGPFGVLS 
      YPLLVSVLK MSYSMLCML TS
    CDS sequence
    • >TC034856 RA
      atgtttcga aatgagtta caagatgat ggagatttg 
      cggactgaa atttttcat ctgaagaaa aatttatcc 
      aacattagc accgaaaat cgtatgcta aaggtgaaa 
      atacgcaaa ttgcagaat gaaattatt aaaaaggat 
      aaacaaatt gatgctata ttggatcca aaaaagaat 
      aaatattcg gatgattta aactgggac aatgaaatt 
      ggacaaatt caaattaaa gtccctact tcaaaaaat 
      gttaaacat acaatattt aatgatgaa ataaagaaa 
      aaaacaaac aggagctta tcatggact tgtgaagct 
      gaatcttca gtcgaattt aataaaaaa catcaaaat 
      gaaagaagt aaaagtgga ggaaaagat caatacaaa 
      aattttctt tcaacaatt aaagattca aattctcga 
      caaacaagt tctcggaaa tcaagtccg gaaagtgat 
      cttgaacga attgaactt ctttgtgaa agtccacat 
      gaagaaatt caacaactg cattcatat tttctcgac 
      attattgca gaactaggt cacttaaaa aatacagtg 
      actgaatta caaattgat aaaaataaa actgactct 
      gcgataaaa aaagaaaat tttacaatc gatatgtta 
      gccaaagaa ctaaaaaac ttgagactc aataaacca 
      aaaccaaac agaaattat agtagaaga ttccacagt 
      gcagatcta gttgcaaaa gtgtatacg gcaaataag 
      gtgaccaac aaaagtgta aaagaaaat aaaagcaaa 
      gataaaacc aaagggaag aaaacagga tctgagggc 
      acaaatggc ccaaaattc aagttcaaa aataataag 
      actcaagga accaaaagt gttatggtt caagtagac 
      gatttaaat gacaatacg gaattttcc aatcagtgt 
      ctttacatt gaaagagat aatattaca ataaaggaa 
      aaatcaaaa tcacctgat gacagagaa gatgaagtc 
      ttgacctta atgttgact acaagtgtt tggtttttt 
      tgcatcatt atgcacata attatgtca acacgaagt 
      catcttgat ttacatgtt agcgaagat gttgcgtac 
      attgttgtt ttttgcggt ctttattac atcaccttc 
      aactatgtt aaaaaccaa cacaaaatt gcattacta 
      cttcgggat ttatcaaat tttgacaaa tttggagtc 
      ccacctggt tatgaaaat gtcgaaacg aagttgggt 
      ttctattcg aaatttctc ttcgcttat gtgactgta 
      gctggagtt gtttataat tttatgcga tttattgag 
      aaaggagag tgtgaaaat atgacaggg aaagacttt 
      aatgaagtt tgtggttta atgtcacca ctttggata 
      ccgataagt ttgaacaac aattcccta gtttattgt 
      gccattttt ttgtatgtc ttgatttgc tcgcagatg 
      gttatcaaa gttggttta atgatatcg tatcatgcg 
      acagaaatg gcacatcat attatctta agaatcgat 
      catttgaaa ttaatgatt gtgaaatgt tttgattta 
      gagtataac gaacttcaa cgaagaaaa ttgagacaa 
      tgtatactg tatcacaca gaaatactt gattttgct 
      tcgaggtta gatgaatgt ttttcaact ggaatgttc 
      acacatatt ttcatgact ggagcaatt tttgcatgc 
      attgaaaaa cagtttatt gatggtgaa catcaattt 
      tgcgccgct gtacatttt attggatgg gttttgacg 
      ctatttgtt gcttgtctt ggagggcaa catctaatc 
      aatgcttgg tataaagct gataaagaa ctacgtaaa 
      gatttatta tttatgctt atgaggtgt caaaaaagt 
      ttaaaaatt agagctgga ccatttggt gttttatcg 
      tatccactt ctagtgtcg gtgctaaaa atgtcctac 
      tctatgctt tgtatgctt acgtcttaa
    mRNA sequence
    • >TC034856 RA
      tgctaatta cattgtaaa atttgtttg atcaatact 
      taaattaca aacaaaaca aacgtaact tcgtcttcg 
      gctacaatg tttcgaaat gagttacaa gatgatgga 
      gatttgcgg actgaaatt tttcatctg aagaaaaat 
      ttatccaac attagcacc gaaaatcgt atgctaaag 
      gtgaaaata cgcaaattg cagaatgaa attattaaa 
      aaggataaa caaattgat gctatattg gatccaaaa 
      aagaataaa tattcggat gatttaaac tgggacaat 
      gaaattgga caaattcaa attaaagtc cctacttca 
      aaaaatgtt aaacataca atatttaat gatgaaata 
      aagaaaaaa acaaacagg agcttatca tggacttgt 
      gaagctgaa tcttcagtc gaatttaat aaaaaacat 
      caaaatgaa agaagtaaa agtggagga aaagatcaa 
      tacaaaaat tttctttca acaattaaa gattcaaat 
      tctcgacaa acaagttct cggaaatca agtccggaa 
      agtgatctt gaacgaatt gaacttctt tgtgaaagt 
      ccacatgaa gaaattcaa caactgcat tcatatttt 
      ctcgacatt attgcagaa ctaggtcac ttaaaaaat 
      acagtgact gaattacaa attgataaa aataaaact 
      gactctgcg ataaaaaaa gaaaatttt acaatcgat 
      atgttagcc aaagaacta aaaaacttg agactcaat 
      aaaccaaaa ccaaacaga aattatagt agaagattc 
      cacagtgca gatctagtt gcaaaagtg tatacggca 
      aataaggtg accaacaaa agtgtaaaa gaaaataaa 
      agcaaagat aaaaccaaa gggaagaaa acaggatct 
      gagggcaca aatggccca aaattcaag ttcaaaaat 
      aataagact caaggaacc aaaagtgtt atggttcaa 
      gtagacgat ttaaatgac aatacggaa ttttccaat 
      cagtgtctt tacattgaa agagataat attacaata 
      aaggaaaaa tcaaaatca cctgatgac agagaagat 
      gaagtcttg accttaatg ttgactaca agtgtttgg 
      tttttttgc atcattatg cacataatt atgtcaaca 
      cgaagtcat cttgattta catgttagc gaagatgtt 
      gcgtacatt gttgttttt tgcggtctt tattacatc 
      accttcaac tatgttaaa aaccaacac aaaattgca 
      ttactactt cgggattta tcaaatttt gacaaattt 
      ggagtccca cctggttat gaaaatgtc gaaacgaag 
      ttgggtttc tattcgaaa tttctcttc gcttatgtg 
      actgtagct ggagttgtt tataatttt atgcgattt 
      attgagaaa ggagagtgt gaaaatatg acagggaaa 
      gactttaat gaagtttgt ggtttaatg tcaccactt 
      tggataccg ataagtttg aacaacaat tccctagtt 
      tattgtgcc atttttttg tatgtcttg atttgctcg 
      cagatggtt atcaaagtt ggtttaatg atatcgtat 
      catgcgaca gaaatggca catcatatt atcttaaga 
      atcgatcat ttgaaatta atgattgtg aaatgtttt 
      gatttagag tataacgaa cttcaacga agaaaattg 
      agacaatgt atactgtat cacacagaa atacttgat 
      tttgcttcg aggttagat gaatgtttt tcaactgga 
      atgttcaca catattttc atgactgga gcaattttt 
      gcatgcatt gaaaaacag tttattgat ggtgaacat 
      caattttgc gccgctgta cattttatt ggatgggtt 
      ttgacgcta tttgttgct tgtcttgga gggcaacat 
      ctaatcaat gcttggtat aaagctgat aaagaacta 
      cgtaaagat ttattattt atgcttatg aggtgtcaa 
      aaaagttta aaaattaga gctggacca tttggtgtt 
      ttatcgtat ccacttcta gtgtcggtg ctaaaaatg 
      tcctactct atgctttgt atgcttacg tcttaaatc 
      tagttgcaa gtttggcta ataaaaatt tatctgcac 
      t
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