Status
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Gene information
General information
TC003711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13547724..13554228
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003711
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06164

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003711 RA
      MSTKLKLYL GRKVIDSLS KSTKFTPIE LLPLIHVGN 
      TTLNNTFEL HLPLSTLED HFGVPNVNE ILKIRPDDI 
      IKNIETKKD RANRKICFE IDRTIFIQD VIENSPNPD 
      LNLKPRNIV VEFSSPNIA KPFHMGHLR STIIGNFIG 
      NLNSFLNNK VTKLNYLGD WGTQFGFIK VGVDELQHT 
      QEAIKNNPL KLLYESYVH ANKLAEKDP TVADRARQE 
      FNKLEKGAP EGLEYWKEY MQYSQNELE ATYKRLGVV 
      FDEYNFESN YSAKDIQNV IDILSSKHI VQKEGDGKL 
      SVDVNKRKV SIMKSDGST LYLTRDIAA AIDRYNRYS 
      FDKMLYIVD NSQSDHFNN LKAILHKMS LPWAHKLQH 
      VKFGRIRGM STRKGTAVF LRDILDECR ELMIKKQVE 
      SPNTRVPIE DSAVSDILG VSCVIVNDL KQRRQKDYE 
      FTWDSVLQV QGDTGVKLQ YTHCRLYSL ERNSGATRP 
      KKVIPEVLK EPEATALVR EIARFHEIL NRSNEQLEA 
      CILVTYLFR LCNQINKAL KVLPVKNTD PEIASQRLL 
      LFITARQVL GKGMSILGL HPLDEM
    CDS sequence
    • >TC003711 RA
      atgtcaaca aaattaaaa ctgtatcta ggccgaaag 
      gtgattgat tcactcagt aagtcaacg aaatttacg 
      cccattgaa ctccttccc cttattcat gtcggaaac 
      accactttg aataacact tttgagtta cacttgcct 
      ctgagcacc cttgaagac cattttggc gtccccaat 
      gtcaacgaa atccttaaa atccgcccc gatgatata 
      attaaaaac attgaaaca aagaaagac cgcgcaaat 
      cgaaaaatt tgcttcgaa atagaccgc acaattttt 
      atccaagat gtgattgaa aacagcccc aaccctgac 
      ttaaaccta aaacctaga aacatagtc gttgaattt 
      agttctcca aacattgct aaaccattc cacatggga 
      catttgcgc tcaaccata atcggtaat tttattggc 
      aatttaaat tcatttctg aacaataaa gtcacaaaa 
      ctcaactat ttgggcgac tggggaaca cagtttggg 
      tttattaaa gtgggggtt gacgagctt caacacaca 
      caggaagcg atcaaaaat aatccgctc aaactttta 
      tacgagtcc tatgtgcac gcaaataaa cttgcagaa 
      aaagatccc acagttgca gacagggcg agacaggag 
      ttcaataaa ctggaaaaa ggggcaccc gaggggttg 
      gagtattgg aaagaatac atgcaatat agtcaaaat 
      gaattagag gcgacttac aagagatta ggggttgtt 
      tttgatgag tacaatttt gagtcgaat tatagtgcc 
      aaagatatt caaaatgtg atagatatt ttaagtagt 
      aagcacatt gtacagaag gaaggcgac ggaaagttg 
      agtgttgat gtaaataaa cggaaagtt tcaataatg 
      aagagtgat ggatccact ttgtattta acgagagat 
      atagcggcc gccatagat aggtataat agatacagc 
      tttgataaa atgctttac atcgtggat aatagtcaa 
      agcgaccat ttcaacaat cttaaagcg attttacac 
      aaaatgagc cttccttgg gcccataaa ttacaacac 
      gttaaattt gggagaatt cgaggcatg agtacgaga 
      aaaggaaca gcagtcttt ttgagagac attctcgac 
      gaatgtcgg gagttaatg ataaagaaa caggtggag 
      tccccaaac acgcgagtc cctattgag gacagtgcc 
      gtgtcagac atacttggc gtttcttgc gttatagtc 
      aacgacttg aagcaacgg agacaaaag gattacgag 
      ttcacctgg gacagtgtt ttacaagtc caaggcgac 
      acgggtgtt aaattacaa tacacccac tgccgcctc 
      tatagtctc gaacgcaat tctggtgca acacgtccc 
      aaaaaagta attcccgaa gtattaaaa gagccagaa 
      gccactgcc ttagttcga gaaatagcc cggtttcat 
      gagatttta aaccggtcc aatgaacag ctcgaagct 
      tgcattctt gtaacttac cttttccgc ctgtgtaat 
      caaatcaac aaagcactg aaagtgtta ccagtcaag 
      aatacggac cccgaaatc gcctcacag aggctactt 
      ctgttcata acagcgcga caggtccta ggaaaggga 
      atgagcata ctaggatta cacccttta gacgaaatg 
      taa
    mRNA sequence
    • >TC003711 RA
      cacatgttg ccattatca taacctcaa aatccgtaa 
      tttcataat tttcggctt tatgtaagt aaattctag 
      tgtaaaaac gtgtgaaaa ggaatgtca acaaaatta 
      aaactgtat ctaggccga aaggtgatt gattcactc 
      agtaagtca acgaaattt acgcccatt gaactcctt 
      ccccttatt catgtcgga aacaccact ttgaataac 
      acttttgag ttacacttg cctctgagc acccttgaa 
      gaccatttt ggcgtcccc aatgtcaac gaaatcctt 
      aaaatccgc cccgatgat ataattaaa aacattgaa 
      acaaagaaa gaccgcgca aatcgaaaa atttgcttc 
      gaaatagac cgcacaatt tttatccaa gatgtgatt 
      gaaaacagc cccaaccct gacttaaac ctaaaacct 
      agaaacata gtcgttgaa tttagttct ccaaacatt 
      gctaaacca ttccacatg ggacatttg cgctcaacc 
      ataatcggt aattttatt ggcaattta aattcattt 
      ctgaacaat aaagtcaca aaactcaac tatttgggc 
      gactgggga acacagttt gggtttatt aaagtgggg 
      gttgacgag cttcaacac acacaggaa gcgatcaaa 
      aataatccg ctcaaactt ttatacgag tcctatgtg 
      cacgcaaat aaacttgca gaaaaagat cccacagtt 
      gcagacagg gcgagacag gagttcaat aaactggaa 
      aaaggggca cccgagggg ttggagtat tggaaagaa 
      tacatgcaa tatagtcaa aatgaatta gaggcgact 
      tacaagaga ttaggggtt gtttttgat gagtacaat 
      tttgagtcg aattatagt gccaaagat attcaaaat 
      gtgatagat attttaagt agtaagcac attgtacag 
      aaggaaggc gacggaaag ttgagtgtt gatgtaaat 
      aaacggaaa gtttcaata atgaagagt gatggatcc 
      actttgtat ttaacgaga gatatagcg gccgccata 
      gataggtat aatagatac agctttgat aaaatgctt 
      tacatcgtg gataatagt caaagcgac catttcaac 
      aatcttaaa gcgatttta cacaaaatg agccttcct 
      tgggcccat aaattacaa cacgttaaa tttgggaga 
      attcgaggc atgagtacg agaaaagga acagcagtc 
      tttttgaga gacattctc gacgaatgt cgggagtta 
      atgataaag aaacaggtg gagtcccca aacacgcga 
      gtccctatt gaggacagt gccgtgtca gacatactt 
      ggcgtttct tgcgttata gtcaacgac ttgaagcaa 
      cggagacaa aaggattac gagttcacc tgggacagt 
      gttttacaa gtccaaggc gacacgggt gttaaatta 
      caatacacc cactgccgc ctctatagt ctcgaacgc 
      aattctggt gcaacacgt cccaaaaaa gtaattccc 
      gaagtatta aaagagcca gaagccact gccttagtt 
      cgagaaata gcccggttt catgagatt ttaaaccgg 
      tccaatgaa cagctcgaa gcttgcatt cttgtaact 
      taccttttc cgcctgtgt aatcaaatc aacaaagca 
      ctgaaagtg ttaccagtc aagaatacg gaccccgaa 
      atcgcctca cagaggcta cttctgttc ataacagcg 
      cgacaggtc ctaggaaag ggaatgagc atactagga 
      ttacaccct ttagacgaa atgtaaatt actttatta 
      aactgattg tgatacaac taacgtttc ttccaagtg 
      aatttcctt cttgctcct tgctgtcct ggcttcttc 
      ctctctttc gtgcggtag tcccttgtc agcaaacca 
      gctaaaaaa ttgcatgaa aaaaatgga ccgagagat 
      tgaatccac aaacctaat cgcatcttt tcgatcatg 
      tcttgatcg acaaaactc ctcagaccc caggctatg 
      ccccaacgg atcgcgcca gcttgactt gaaggaccg 
      ccaccttcc acttccgcc tcaatgtcc actttattc 
      aacatgttc gaaaacaag agagggaac aatacctaa 
      aatcatgtt cattatgta a
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