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Gene information
General information
TC002934 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6047452..6050908
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002934
    iBeetle screen iB_06162

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002934 RA
      MSTILTDLT SALEANNEK DIIEKLRRI VAQGEDIEL 
      QNNNILKRL ITLNNEKIT QAFVEVAAE LSKQERNRK 
      FFTDKEVIE YLLNLLDKP EESLVIGAI RALGNICYE 
      NDDARRIID KQGLATVIS ILAKDNNRK NNLLTLKVS 
      GFLVNLLMS NDDLQKAAL KFNIVDVIE KLLNKYLKS 
      FNENQMLFA FLLTILNHV IDYIDDQNI PFTESLCCT 
      IIDIFKLST TPEISVLCL EILHGQSEK EDIKTILAK 
      KGICELVFE LVEKYRSQV NDEESRSVM KMACDIIVI 
      ILTGDDCMN LLYNNGQGK VYENMVTWL DSDDPDLLS 
      TGVLAIGNF ARKDTHCIQ MVQKGISKK LIALLAKYN 
      TRESDIKVQ HALLSTLKN LVIPQQNKR IVLEEGLIE 
      VIYPMINLE QYLVIFKLL GTFRMVIDG QEKAALDLI 
      SRKEFLEKL IYWCYNSDH LGVRGEIPR LLSWLIKHC 
      HSFKPFHAL LQVRDSVKC LVEMMSSNH AVMQNESFY 
      ALNLLCIGC GVSHENDSS NGVNGDHAN EDGVLFEQL 
      IDAEIGKNL SFVVNKYSD KMDKQTIEN LVTLLEQLI 
      NSRAVSSHL KSNNVGEAL AKLASNSNA QSLSDKLNH 
      VMSEINKN
    CDS sequence
    • >TC002934 RA
      atgagcact attttaacc gatttaaca tctgcatta 
      gaagcaaat aacgagaag gacataatc gagaagttg 
      aggcgcatt gtggctcag ggcgaagac atcgaacta 
      caaaacaat aatattttg aaacgtctt attacatta 
      aataacgag aaaataaca caagccttt gtggaagta 
      gcggcggaa ttatcaaag caggaacgc aaccgaaag 
      ttttttact gacaaggaa gtcatcgag taccttttg 
      aatttatta gataagccc gaggagtct ttggtgatc 
      ggcgcaata agagcactg ggcaatatt tgctacgaa 
      aatgacgac gcgcggaga attatcgac aaacagggg 
      ctggccacc gtcatatca atcctcgcg aaagacaac 
      aacaggaaa aataattta ctcaccttg aaagttagc 
      gggtttctc gtcaatttg ttgatgtcg aacgatgat 
      ctacaaaaa gctgccctc aaattcaac atcgttgat 
      gtgatcgaa aaactgtta aataaatac ttgaagtcg 
      ttcaatgaa aatcagatg ctttttgcg ttccttttg 
      acgattcta aaccatgtg attgactac attgatgac 
      cagaatatt cccttcacg gaaagcctt tgctgtacg 
      attatagac attttcaag ctttccaca acacccgaa 
      atttccgtt ttgtgttta gaaatattg catggacag 
      tcagagaaa gaggacatt aaaacaatt ctggcgaaa 
      aaaggcatc tgtgagctg gtttttgaa ctagttgag 
      aagtaccga agccaagtc aatgatgaa gaaagccga 
      tccgttatg aaaatggct tgtgatata attgtaatt 
      atcttaact ggagatgat tgtatgaat ttgttatac 
      aacaacggc caagggaag gtgtacgaa aatatggtc 
      acttggctt gattcagat gatccagat ttactaagt 
      acaggtgtt ttggcaatt ggaaatttt gccaggaaa 
      gacacccac tgtattcaa atggtccag aaaggcatt 
      tcgaaaaaa ctcatagct ttattggcc aaatacaac 
      acgcgtgaa agtgatatc aaagtgcag catgcctta 
      ctaagcacc cttaaaaac ctggtgata cctcaacaa 
      aacaaacgc atcgttttg gaagagggc ttgatcgag 
      gtgatttac cccatgatc aatttggag cagtatttg 
      gtcattttc aaactgctg gggactttc agaatggtc 
      attgatggc caagaaaaa gcagcgttg gatttaatt 
      tcgcgtaag gaattttta gagaagtta atctactgg 
      tgctataac tcggaccat ttgggggtc cgtggcgag 
      attcctcgg ttactatca tggttaata aaacactgt 
      cactccttt aagcccttt catgcctta ctacaggtc 
      agagatagt gtgaaatgt ctggtagag atgatgtcc 
      tccaatcac gcggtcatg caaaacgag tccttttac 
      gccttgaat ttgctgtgt ataggctgc ggggtgtcg 
      cacgaaaac gacagttcc aatggggta aacggtgat 
      cacgcgaat gaggacgga gttttgttt gagcaactg 
      attgatgcc gagattggt aaaaatctc agttttgtt 
      gtcaataaa tatagtgat aagatggat aagcaaacg 
      attgagaat ttagtaacg ctgcttgag cagttgatc 
      aactcaagg gctgtttca agtcacctc aagtcgaac 
      aacgttggg gaagcactg gcgaaactt gcgtcaaat 
      tccaacgcg caaagtctc tctgataaa ttgaatcac 
      gtcatgagc gaaattaat aaaaactga
    mRNA sequence
    • >TC002934 RA
      attcacaaa ttttcgaac tgattttgt ggttatgtt 
      gtccaaaat tgtcgaatt gttttgatc tgttttcaa 
      aattcgagt gcgaaatga gcactattt taaccgatt 
      taacatctg cattagaag caaataacg agaaggaca 
      taatcgaga agttgaggc gcattgtgg ctcagggcg 
      aagacatcg aactacaaa acaataata ttttgaaac 
      gtcttatta cattaaata acgagaaaa taacacaag 
      cctttgtgg aagtagcgg cggaattat caaagcagg 
      aacgcaacc gaaagtttt ttactgaca aggaagtca 
      tcgagtacc ttttgaatt tattagata agcccgagg 
      agtctttgg tgatcggcg caataagag cactgggca 
      atatttgct acgaaaatg acgacgcgc ggagaatta 
      tcgacaaac aggggctgg ccaccgtca tatcaatcc 
      tcgcgaaag acaacaaca ggaaaaata atttactca 
      ccttgaaag ttagcgggt ttctcgtca atttgttga 
      tgtcgaacg atgatctac aaaaagctg ccctcaaat 
      tcaacatcg ttgatgtga tcgaaaaac tgttaaata 
      aatacttga agtcgttca atgaaaatc agatgcttt 
      ttgcgttcc ttttgacga ttctaaacc atgtgattg 
      actacattg atgaccaga atattccct tcacggaaa 
      gcctttgct gtacgatta tagacattt tcaagcttt 
      ccacaacac ccgaaattt ccgttttgt gtttagaaa 
      tattgcatg gacagtcag agaaagagg acattaaaa 
      caattctgg cgaaaaaag gcatctgtg agctggttt 
      ttgaactag ttgagaagt accgaagcc aagtcaatg 
      atgaagaaa gccgatccg ttatgaaaa tggcttgtg 
      atataattg taattatct taactggag atgattgta 
      tgaatttgt tatacaaca acggccaag ggaaggtgt 
      acgaaaata tggtcactt ggcttgatt cagatgatc 
      cagatttac taagtacag gtgttttgg caattggaa 
      attttgcca ggaaagaca cccactgta ttcaaatgg 
      tccagaaag gcatttcga aaaaactca tagctttat 
      tggccaaat acaacacgc gtgaaagtg atatcaaag 
      tgcagcatg ccttactaa gcaccctta aaaacctgg 
      tgatacctc aacaaaaca aacgcatcg ttttggaag 
      agggcttga tcgaggtga tttacccca tgatcaatt 
      tggagcagt atttggtca ttttcaaac tgctgggga 
      ctttcagaa tggtcattg atggccaag aaaaagcag 
      cgttggatt taatttcgc gtaaggaat ttttagaga 
      agttaatct actggtgct ataactcgg accatttgg 
      gggtccgtg gcgagattc ctcggttac tatcatggt 
      taataaaac actgtcact cctttaagc cctttcatg 
      ccttactac aggtcagag atagtgtga aatgtctgg 
      tagagatga tgtcctcca atcacgcgg tcatgcaaa 
      acgagtcct tttacgcct tgaatttgc tgtgtatag 
      gctgcgggg tgtcgcacg aaaacgaca gttccaatg 
      gggtaaacg gtgatcacg cgaatgagg acggagttt 
      tgtttgagc aactgattg atgccgaga ttggtaaaa 
      atctcagtt ttgttgtca ataaatata gtgataaga 
      tggataagc aaacgattg agaatttag taacgctgc 
      ttgagcagt tgatcaact caagggctg tttcaagtc 
      acctcaagt cgaacaacg ttggggaag cactggcga 
      aacttgcgt caaattcca acgcgcaaa gtctctctg 
      ataaattga atcacgtca tgagcgaaa ttaataaaa 
      actgagtga tccaacgtg tatagaact ttagattcc 
      ttactaggt attaaaata gttactatt ttccattat 
      cgtacaatt ttgatgtat tacgaatag ttgccttta 
      atgcatgtg ttaaagtga ttttaatat gtttatcct 
      cgatccagc ttatgaaaa atcgctttc atagcgttt 
      taactttga atagtctta tgaagcacg tcaaatttt 
      agcgttgtt taaggttca tccgtgtgt ttctctcgc 
      aaaaacgta agatatttt ttagacaag tcgatttta 
      ccgggttgt gaaccccac ttccaatag gttgtagaa 
      agtatttgg acattatgg ctgcgtaaa attattttg 
      aaattagtt gatacttaa ttttattta ttcagtaca 
      gtttagatg tcactacaa tcataatta ctagcgtac 
      aaataattt atttcaatt acttatatc taaattggc 
      ttgtgtgaa acagacaag ttgtagaaa aaaatcaca 
      ttttagtgt ctttaaaca tcacttcat gtttttaca 
      caaaatata attgttcca caaaaataa taataaact 
      tgcattacg aaaccggtc tatattttt cgataaacc 
      aaagacgta actgtattt cctgttttc cccccgaac 
      tgaaattga ggcgttgga atattatcg acacaaaca 
      aaatgattt ctaaagtgc tcatttaat tcatccttc 
      gtccaatta catgtcgtg ataataaaa aacccgttc 
      gggtctaaa gccttgtga acattttct aaatacttt 
      cctctaaat tctttaata tttgcattc aaactgatc 
      gcgtcatat gttccttta tcatggata atgtcgaac 
      tgaccgtcc agatgttcc aaaatatca cattcggaa 
      tattgtatt tcaaatccc tgcttttgg gctattgat 
      tttgctaaa gttatggca tcttttgag taatcaacc 
      ccgaataaa tttgtatac ccttccgtg gccagttct 
      attaaaaac ataccgttc ccacaacca acgtcaaga 
      atttttgat tccttagtt gcggtttca tttttttcg 
      atccatttt atgagtcgc tcgactgtg tcttcacca 
      aaccaaatt tctccagga tcgccatgg tcttgaaag 
      ttctctatc tcttccttg tagcgatta tcccaatac 
      tcttggctc ccaagctca gattcttga agctcttcc 
      attgcaact ttaccaaga caaacaatt caaatttcc 
      cgcgaaata ttcaatcaa gtttttagg ttacgcaag 
      tgttaacct aaaaaaata attcgattt ctaaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY