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Gene information
General information
TC002934 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6047452..6050908
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002934
    iBeetle screen iB_06162

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002934 RA
      MSTILTDLT SALEANNEK DIIEKLRRI VAQGEDIEL 
QNNNILKRL ITLNNEKIT QAFVEVAAE LSKQERNRK 
FFTDKEVIE YLLNLLDKP EESLVIGAI RALGNICYE 
NDDARRIID KQGLATVIS ILAKDNNRK NNLLTLKVS 
GFLVNLLMS NDDLQKAAL KFNIVDVIE KLLNKYLKS 
FNENQMLFA FLLTILNHV IDYIDDQNI PFTESLCCT 
IIDIFKLST TPEISVLCL EILHGQSEK EDIKTILAK 
KGICELVFE LVEKYRSQV NDEESRSVM KMACDIIVI 
ILTGDDCMN LLYNNGQGK VYENMVTWL DSDDPDLLS 
TGVLAIGNF ARKDTHCIQ MVQKGISKK LIALLAKYN 
TRESDIKVQ HALLSTLKN LVIPQQNKR IVLEEGLIE 
VIYPMINLE QYLVIFKLL GTFRMVIDG QEKAALDLI 
SRKEFLEKL IYWCYNSDH LGVRGEIPR LLSWLIKHC 
HSFKPFHAL LQVRDSVKC LVEMMSSNH AVMQNESFY 
ALNLLCIGC GVSHENDSS NGVNGDHAN EDGVLFEQL 
IDAEIGKNL SFVVNKYSD KMDKQTIEN LVTLLEQLI 
NSRAVSSHL KSNNVGEAL AKLASNSNA QSLSDKLNH 
VMSEINKN
    CDS sequence
    • >TC002934 RA
      atgagcact attttaacc gatttaaca tctgcatta 
gaagcaaat aacgagaag gacataatc gagaagttg 
aggcgcatt gtggctcag ggcgaagac atcgaacta 
caaaacaat aatattttg aaacgtctt attacatta 
aataacgag aaaataaca caagccttt gtggaagta 
gcggcggaa ttatcaaag caggaacgc aaccgaaag 
ttttttact gacaaggaa gtcatcgag taccttttg 
aatttatta gataagccc gaggagtct ttggtgatc 
ggcgcaata agagcactg ggcaatatt tgctacgaa 
aatgacgac gcgcggaga attatcgac aaacagggg 
ctggccacc gtcatatca atcctcgcg aaagacaac 
aacaggaaa aataattta ctcaccttg aaagttagc 
gggtttctc gtcaatttg ttgatgtcg aacgatgat 
ctacaaaaa gctgccctc aaattcaac atcgttgat 
gtgatcgaa aaactgtta aataaatac ttgaagtcg 
ttcaatgaa aatcagatg ctttttgcg ttccttttg 
acgattcta aaccatgtg attgactac attgatgac 
cagaatatt cccttcacg gaaagcctt tgctgtacg 
attatagac attttcaag ctttccaca acacccgaa 
atttccgtt ttgtgttta gaaatattg catggacag 
tcagagaaa gaggacatt aaaacaatt ctggcgaaa 
aaaggcatc tgtgagctg gtttttgaa ctagttgag 
aagtaccga agccaagtc aatgatgaa gaaagccga 
tccgttatg aaaatggct tgtgatata attgtaatt 
atcttaact ggagatgat tgtatgaat ttgttatac 
aacaacggc caagggaag gtgtacgaa aatatggtc 
acttggctt gattcagat gatccagat ttactaagt 
acaggtgtt ttggcaatt ggaaatttt gccaggaaa 
gacacccac tgtattcaa atggtccag aaaggcatt 
tcgaaaaaa ctcatagct ttattggcc aaatacaac 
acgcgtgaa agtgatatc aaagtgcag catgcctta 
ctaagcacc cttaaaaac ctggtgata cctcaacaa 
aacaaacgc atcgttttg gaagagggc ttgatcgag 
gtgatttac cccatgatc aatttggag cagtatttg 
gtcattttc aaactgctg gggactttc agaatggtc 
attgatggc caagaaaaa gcagcgttg gatttaatt 
tcgcgtaag gaattttta gagaagtta atctactgg 
tgctataac tcggaccat ttgggggtc cgtggcgag 
attcctcgg ttactatca tggttaata aaacactgt 
cactccttt aagcccttt catgcctta ctacaggtc 
agagatagt gtgaaatgt ctggtagag atgatgtcc 
tccaatcac gcggtcatg caaaacgag tccttttac 
gccttgaat ttgctgtgt ataggctgc ggggtgtcg 
cacgaaaac gacagttcc aatggggta aacggtgat 
cacgcgaat gaggacgga gttttgttt gagcaactg 
attgatgcc gagattggt aaaaatctc agttttgtt 
gtcaataaa tatagtgat aagatggat aagcaaacg 
attgagaat ttagtaacg ctgcttgag cagttgatc 
aactcaagg gctgtttca agtcacctc aagtcgaac 
aacgttggg gaagcactg gcgaaactt gcgtcaaat 
tccaacgcg caaagtctc tctgataaa ttgaatcac 
gtcatgagc gaaattaat aaaaactga
    mRNA sequence
    • >TC002934 RA
      attcacaaa ttttcgaac tgattttgt ggttatgtt 
gtccaaaat tgtcgaatt gttttgatc tgttttcaa 
aattcgagt gcgaaatga gcactattt taaccgatt 
taacatctg cattagaag caaataacg agaaggaca 
taatcgaga agttgaggc gcattgtgg ctcagggcg 
aagacatcg aactacaaa acaataata ttttgaaac 
gtcttatta cattaaata acgagaaaa taacacaag 
cctttgtgg aagtagcgg cggaattat caaagcagg 
aacgcaacc gaaagtttt ttactgaca aggaagtca 
tcgagtacc ttttgaatt tattagata agcccgagg 
agtctttgg tgatcggcg caataagag cactgggca 
atatttgct acgaaaatg acgacgcgc ggagaatta 
tcgacaaac aggggctgg ccaccgtca tatcaatcc 
tcgcgaaag acaacaaca ggaaaaata atttactca 
ccttgaaag ttagcgggt ttctcgtca atttgttga 
tgtcgaacg atgatctac aaaaagctg ccctcaaat 
tcaacatcg ttgatgtga tcgaaaaac tgttaaata 
aatacttga agtcgttca atgaaaatc agatgcttt 
ttgcgttcc ttttgacga ttctaaacc atgtgattg 
actacattg atgaccaga atattccct tcacggaaa 
gcctttgct gtacgatta tagacattt tcaagcttt 
ccacaacac ccgaaattt ccgttttgt gtttagaaa 
tattgcatg gacagtcag agaaagagg acattaaaa 
caattctgg cgaaaaaag gcatctgtg agctggttt 
ttgaactag ttgagaagt accgaagcc aagtcaatg 
atgaagaaa gccgatccg ttatgaaaa tggcttgtg 
atataattg taattatct taactggag atgattgta 
tgaatttgt tatacaaca acggccaag ggaaggtgt 
acgaaaata tggtcactt ggcttgatt cagatgatc 
cagatttac taagtacag gtgttttgg caattggaa 
attttgcca ggaaagaca cccactgta ttcaaatgg 
tccagaaag gcatttcga aaaaactca tagctttat 
tggccaaat acaacacgc gtgaaagtg atatcaaag 
tgcagcatg ccttactaa gcaccctta aaaacctgg 
tgatacctc aacaaaaca aacgcatcg ttttggaag 
agggcttga tcgaggtga tttacccca tgatcaatt 
tggagcagt atttggtca ttttcaaac tgctgggga 
ctttcagaa tggtcattg atggccaag aaaaagcag 
cgttggatt taatttcgc gtaaggaat ttttagaga 
agttaatct actggtgct ataactcgg accatttgg 
gggtccgtg gcgagattc ctcggttac tatcatggt 
taataaaac actgtcact cctttaagc cctttcatg 
ccttactac aggtcagag atagtgtga aatgtctgg 
tagagatga tgtcctcca atcacgcgg tcatgcaaa 
acgagtcct tttacgcct tgaatttgc tgtgtatag 
gctgcgggg tgtcgcacg aaaacgaca gttccaatg 
gggtaaacg gtgatcacg cgaatgagg acggagttt 
tgtttgagc aactgattg atgccgaga ttggtaaaa 
atctcagtt ttgttgtca ataaatata gtgataaga 
tggataagc aaacgattg agaatttag taacgctgc 
ttgagcagt tgatcaact caagggctg tttcaagtc 
acctcaagt cgaacaacg ttggggaag cactggcga 
aacttgcgt caaattcca acgcgcaaa gtctctctg 
ataaattga atcacgtca tgagcgaaa ttaataaaa 
actgagtga tccaacgtg tatagaact ttagattcc 
ttactaggt attaaaata gttactatt ttccattat 
cgtacaatt ttgatgtat tacgaatag ttgccttta 
atgcatgtg ttaaagtga ttttaatat gtttatcct 
cgatccagc ttatgaaaa atcgctttc atagcgttt 
taactttga atagtctta tgaagcacg tcaaatttt 
agcgttgtt taaggttca tccgtgtgt ttctctcgc 
aaaaacgta agatatttt ttagacaag tcgatttta 
ccgggttgt gaaccccac ttccaatag gttgtagaa 
agtatttgg acattatgg ctgcgtaaa attattttg 
aaattagtt gatacttaa ttttattta ttcagtaca 
gtttagatg tcactacaa tcataatta ctagcgtac 
aaataattt atttcaatt acttatatc taaattggc 
ttgtgtgaa acagacaag ttgtagaaa aaaatcaca 
ttttagtgt ctttaaaca tcacttcat gtttttaca 
caaaatata attgttcca caaaaataa taataaact 
tgcattacg aaaccggtc tatattttt cgataaacc 
aaagacgta actgtattt cctgttttc cccccgaac 
tgaaattga ggcgttgga atattatcg acacaaaca 
aaatgattt ctaaagtgc tcatttaat tcatccttc 
gtccaatta catgtcgtg ataataaaa aacccgttc 
gggtctaaa gccttgtga acattttct aaatacttt 
cctctaaat tctttaata tttgcattc aaactgatc 
gcgtcatat gttccttta tcatggata atgtcgaac 
tgaccgtcc agatgttcc aaaatatca cattcggaa 
tattgtatt tcaaatccc tgcttttgg gctattgat 
tttgctaaa gttatggca tcttttgag taatcaacc 
ccgaataaa tttgtatac ccttccgtg gccagttct 
attaaaaac ataccgttc ccacaacca acgtcaaga 
atttttgat tccttagtt gcggtttca tttttttcg 
atccatttt atgagtcgc tcgactgtg tcttcacca 
aaccaaatt tctccagga tcgccatgg tcttgaaag 
ttctctatc tcttccttg tagcgatta tcccaatac 
tcttggctc ccaagctca gattcttga agctcttcc 
attgcaact ttaccaaga caaacaatt caaatttcc 
cgcgaaata ttcaatcaa gtttttagg ttacgcaag 
tgttaacct aaaaaaata attcgattt ctaaacg
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