| General information | |||
| TC002934 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6047452..6050908 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002934 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06162 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC002934 RA
MSTILTDLT SALEANNEK DIIEKLRRI VAQGEDIEL QNNNILKRL ITLNNEKIT QAFVEVAAE LSKQERNRK FFTDKEVIE YLLNLLDKP EESLVIGAI RALGNICYE NDDARRIID KQGLATVIS ILAKDNNRK NNLLTLKVS GFLVNLLMS NDDLQKAAL KFNIVDVIE KLLNKYLKS FNENQMLFA FLLTILNHV IDYIDDQNI PFTESLCCT IIDIFKLST TPEISVLCL EILHGQSEK EDIKTILAK KGICELVFE LVEKYRSQV NDEESRSVM KMACDIIVI ILTGDDCMN LLYNNGQGK VYENMVTWL DSDDPDLLS TGVLAIGNF ARKDTHCIQ MVQKGISKK LIALLAKYN TRESDIKVQ HALLSTLKN LVIPQQNKR IVLEEGLIE VIYPMINLE QYLVIFKLL GTFRMVIDG QEKAALDLI SRKEFLEKL IYWCYNSDH LGVRGEIPR LLSWLIKHC HSFKPFHAL LQVRDSVKC LVEMMSSNH AVMQNESFY ALNLLCIGC GVSHENDSS NGVNGDHAN EDGVLFEQL IDAEIGKNL SFVVNKYSD KMDKQTIEN LVTLLEQLI NSRAVSSHL KSNNVGEAL AKLASNSNA QSLSDKLNH VMSEINKN
- >TC002934 RA
atgagcact attttaacc gatttaaca tctgcatta gaagcaaat aacgagaag gacataatc gagaagttg aggcgcatt gtggctcag ggcgaagac atcgaacta caaaacaat aatattttg aaacgtctt attacatta aataacgag aaaataaca caagccttt gtggaagta gcggcggaa ttatcaaag caggaacgc aaccgaaag ttttttact gacaaggaa gtcatcgag taccttttg aatttatta gataagccc gaggagtct ttggtgatc ggcgcaata agagcactg ggcaatatt tgctacgaa aatgacgac gcgcggaga attatcgac aaacagggg ctggccacc gtcatatca atcctcgcg aaagacaac aacaggaaa aataattta ctcaccttg aaagttagc gggtttctc gtcaatttg ttgatgtcg aacgatgat ctacaaaaa gctgccctc aaattcaac atcgttgat gtgatcgaa aaactgtta aataaatac ttgaagtcg ttcaatgaa aatcagatg ctttttgcg ttccttttg acgattcta aaccatgtg attgactac attgatgac cagaatatt cccttcacg gaaagcctt tgctgtacg attatagac attttcaag ctttccaca acacccgaa atttccgtt ttgtgttta gaaatattg catggacag tcagagaaa gaggacatt aaaacaatt ctggcgaaa aaaggcatc tgtgagctg gtttttgaa ctagttgag aagtaccga agccaagtc aatgatgaa gaaagccga tccgttatg aaaatggct tgtgatata attgtaatt atcttaact ggagatgat tgtatgaat ttgttatac aacaacggc caagggaag gtgtacgaa aatatggtc acttggctt gattcagat gatccagat ttactaagt acaggtgtt ttggcaatt ggaaatttt gccaggaaa gacacccac tgtattcaa atggtccag aaaggcatt tcgaaaaaa ctcatagct ttattggcc aaatacaac acgcgtgaa agtgatatc aaagtgcag catgcctta ctaagcacc cttaaaaac ctggtgata cctcaacaa aacaaacgc atcgttttg gaagagggc ttgatcgag gtgatttac cccatgatc aatttggag cagtatttg gtcattttc aaactgctg gggactttc agaatggtc attgatggc caagaaaaa gcagcgttg gatttaatt tcgcgtaag gaattttta gagaagtta atctactgg tgctataac tcggaccat ttgggggtc cgtggcgag attcctcgg ttactatca tggttaata aaacactgt cactccttt aagcccttt catgcctta ctacaggtc agagatagt gtgaaatgt ctggtagag atgatgtcc tccaatcac gcggtcatg caaaacgag tccttttac gccttgaat ttgctgtgt ataggctgc ggggtgtcg cacgaaaac gacagttcc aatggggta aacggtgat cacgcgaat gaggacgga gttttgttt gagcaactg attgatgcc gagattggt aaaaatctc agttttgtt gtcaataaa tatagtgat aagatggat aagcaaacg attgagaat ttagtaacg ctgcttgag cagttgatc aactcaagg gctgtttca agtcacctc aagtcgaac aacgttggg gaagcactg gcgaaactt gcgtcaaat tccaacgcg caaagtctc tctgataaa ttgaatcac gtcatgagc gaaattaat aaaaactga
- >TC002934 RA
attcacaaa ttttcgaac tgattttgt ggttatgtt gtccaaaat tgtcgaatt gttttgatc tgttttcaa aattcgagt gcgaaatga gcactattt taaccgatt taacatctg cattagaag caaataacg agaaggaca taatcgaga agttgaggc gcattgtgg ctcagggcg aagacatcg aactacaaa acaataata ttttgaaac gtcttatta cattaaata acgagaaaa taacacaag cctttgtgg aagtagcgg cggaattat caaagcagg aacgcaacc gaaagtttt ttactgaca aggaagtca tcgagtacc ttttgaatt tattagata agcccgagg agtctttgg tgatcggcg caataagag cactgggca atatttgct acgaaaatg acgacgcgc ggagaatta tcgacaaac aggggctgg ccaccgtca tatcaatcc tcgcgaaag acaacaaca ggaaaaata atttactca ccttgaaag ttagcgggt ttctcgtca atttgttga tgtcgaacg atgatctac aaaaagctg ccctcaaat tcaacatcg ttgatgtga tcgaaaaac tgttaaata aatacttga agtcgttca atgaaaatc agatgcttt ttgcgttcc ttttgacga ttctaaacc atgtgattg actacattg atgaccaga atattccct tcacggaaa gcctttgct gtacgatta tagacattt tcaagcttt ccacaacac ccgaaattt ccgttttgt gtttagaaa tattgcatg gacagtcag agaaagagg acattaaaa caattctgg cgaaaaaag gcatctgtg agctggttt ttgaactag ttgagaagt accgaagcc aagtcaatg atgaagaaa gccgatccg ttatgaaaa tggcttgtg atataattg taattatct taactggag atgattgta tgaatttgt tatacaaca acggccaag ggaaggtgt acgaaaata tggtcactt ggcttgatt cagatgatc cagatttac taagtacag gtgttttgg caattggaa attttgcca ggaaagaca cccactgta ttcaaatgg tccagaaag gcatttcga aaaaactca tagctttat tggccaaat acaacacgc gtgaaagtg atatcaaag tgcagcatg ccttactaa gcaccctta aaaacctgg tgatacctc aacaaaaca aacgcatcg ttttggaag agggcttga tcgaggtga tttacccca tgatcaatt tggagcagt atttggtca ttttcaaac tgctgggga ctttcagaa tggtcattg atggccaag aaaaagcag cgttggatt taatttcgc gtaaggaat ttttagaga agttaatct actggtgct ataactcgg accatttgg gggtccgtg gcgagattc ctcggttac tatcatggt taataaaac actgtcact cctttaagc cctttcatg ccttactac aggtcagag atagtgtga aatgtctgg tagagatga tgtcctcca atcacgcgg tcatgcaaa acgagtcct tttacgcct tgaatttgc tgtgtatag gctgcgggg tgtcgcacg aaaacgaca gttccaatg gggtaaacg gtgatcacg cgaatgagg acggagttt tgtttgagc aactgattg atgccgaga ttggtaaaa atctcagtt ttgttgtca ataaatata gtgataaga tggataagc aaacgattg agaatttag taacgctgc ttgagcagt tgatcaact caagggctg tttcaagtc acctcaagt cgaacaacg ttggggaag cactggcga aacttgcgt caaattcca acgcgcaaa gtctctctg ataaattga atcacgtca tgagcgaaa ttaataaaa actgagtga tccaacgtg tatagaact ttagattcc ttactaggt attaaaata gttactatt ttccattat cgtacaatt ttgatgtat tacgaatag ttgccttta atgcatgtg ttaaagtga ttttaatat gtttatcct cgatccagc ttatgaaaa atcgctttc atagcgttt taactttga atagtctta tgaagcacg tcaaatttt agcgttgtt taaggttca tccgtgtgt ttctctcgc aaaaacgta agatatttt ttagacaag tcgatttta ccgggttgt gaaccccac ttccaatag gttgtagaa agtatttgg acattatgg ctgcgtaaa attattttg aaattagtt gatacttaa ttttattta ttcagtaca gtttagatg tcactacaa tcataatta ctagcgtac aaataattt atttcaatt acttatatc taaattggc ttgtgtgaa acagacaag ttgtagaaa aaaatcaca ttttagtgt ctttaaaca tcacttcat gtttttaca caaaatata attgttcca caaaaataa taataaact tgcattacg aaaccggtc tatattttt cgataaacc aaagacgta actgtattt cctgttttc cccccgaac tgaaattga ggcgttgga atattatcg acacaaaca aaatgattt ctaaagtgc tcatttaat tcatccttc gtccaatta catgtcgtg ataataaaa aacccgttc gggtctaaa gccttgtga acattttct aaatacttt cctctaaat tctttaata tttgcattc aaactgatc gcgtcatat gttccttta tcatggata atgtcgaac tgaccgtcc agatgttcc aaaatatca cattcggaa tattgtatt tcaaatccc tgcttttgg gctattgat tttgctaaa gttatggca tcttttgag taatcaacc ccgaataaa tttgtatac ccttccgtg gccagttct attaaaaac ataccgttc ccacaacca acgtcaaga atttttgat tccttagtt gcggtttca tttttttcg atccatttt atgagtcgc tcgactgtg tcttcacca aaccaaatt tctccagga tcgccatgg tcttgaaag ttctctatc tcttccttg tagcgatta tcccaatac tcttggctc ccaagctca gattcttga agctcttcc attgcaact ttaccaaga caaacaatt caaatttcc cgcgaaata ttcaatcaa gtttttagg ttacgcaag tgttaacct aaaaaaata attcgattt ctaaacg
