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Gene information
General information
TC003245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 266179..292666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003245
    iBeetle screen iB_06159

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003245 RA
      MFAVNKSVL IAVLLHSCF ATIYARVIQ EETSSDACP 
      PPQLAFNGT ETEQGEVRI RKDYTHIRE VKFSGSIGP 
      LGEKRLCKI KCIGGQWVG PLCVDNHDN GRFHPLFRS 
      CKLEYINPH LVVTFRNVS IQTAGWVFP HEAKLQIRC 
      RELGLYKLL GTASPRCQN GVWSARLPS CVPTTLLTN 
      FTEDAPPTL LIRIPSGSA SVEPGGELA VFPGSTVHL 
      ECLFSRRLG SPDWTWTSP LGQYLTGWA IAAEERDWK 
      YRLSIYYVK PQDSGTFTC ATPRGITNS LTLQVAAVH 
      CDPISVSGP HLTIRVEGT RLGHTAIFQ CPMGFKVNG 
      AANLTCQAS GKWSAPVPT CELVKCESL DTPGGLYEP 
      HLHLEEHNN SYGGRAIFS CAWGYRLMG PPGIECELN 
      GNWSGPLPK CVPIQCPPP VIPVNGHLI QSEAAGMDG 
      GRYAVGSLV QFACRGAHQ LEGEASIIC TETGFWSHP 
      PPFCKPRCP YLGEPDNGL VAPTKFAYE PGDELQITC 
      SPGFEARLE ARPKCLPDG RWSAPLPNC TNYSEI
    CDS sequence
    • >TC003245 RA
      atgtttgcg gtaaacaaa tcagtgctt atcgctgta 
      cttttacac agttgtttc gccacaatt tacgcaaga 
      gtaatacaa gaagaaaca tcttcggat gcgtgtcca 
      cctcctcaa ttagccttc aacggtaca gaaaccgag 
      cagggagaa gttcgaatt cgcaaggat tacacgcac 
      attcgcgaa gtgaaattt tccggttca attggcccc 
      cttggtgaa aagcgtctt tgcaaaatc aaatgtatc 
      ggaggtcaa tgggtgggc cctctctgt gtcgataat 
      cacgataat ggcaggttt catccgctt ttcaggagc 
      tgtaagctg gagtacatc aatccgcat ctggtggtt 
      accttcagg aatgtctcc attcaaacc gccggatgg 
      gtgtttccc cacgaggcc aaacttcag ataagatgt 
      cgtgagctg gggctgtac aagctcttg gggacggcg 
      tcaccacgc tgtcaaaac ggcgtttgg tcggcgaga 
      ctgccgtca tgtgtgccc acaacgctc ctcaccaat 
      ttcacagag gacgctccg ccgactctc ctaatcagg 
      ataccatcg ggctcggcg tccgtcgaa cccggaggg 
      gaattggcg gtgtttccc ggatcgact gtgcacctg 
      gaatgtctc ttttcccgg cgacttggt tcaccagat 
      tggacgtgg acgtctccc ctgggacaa tatctcacc 
      ggttgggca atagcggca gaagagcgt gattggaag 
      tatagatta tcgatttat tatgtgaag ccccaagac 
      tccggtacg ttcacttgc gccactcct agaggaatc 
      accaacagt ctcactctg caagttgca gccgttcat 
      tgtgacccc atctctgtg tcgggcccc catctcacc 
      ataagggtg gaaggaacc cgtcttgga cacactgcc 
      atcttccaa tgtccaatg ggattcaaa gttaatggt 
      gcagctaat ctcacctgt caggcttcg ggcaaatgg 
      tcagcccct gttccaaca tgcgagctc gtaaaatgc 
      gaatcatta gacaccccc ggcggcctc tacgagccc 
      catttgcac ctcgaagag cacaacaac agttacgga 
      ggccgtgct atcttcagc tgtgcctgg ggctaccgc 
      ctcatgggg cccccaggc atcgaatgc gaactgaac 
      ggtaactgg tcggggcct ttgcccaaa tgcgtccct 
      attcaatgc ccgcccccg gtgattccc gtcaacggc 
      cacctcatc cagtcggag gcggcgggc atggacggg 
      ggccgctat gccgtgggc agtttagta cagtttgcc 
      tgcaggggg gcccaccag ctggagggc gaagccagc 
      attatttgt accgagact gggttttgg tcgcacccg 
      ccccctttc tgcaaaccg cggtgcccc tatttgggg 
      gagcccgac aatgggctg gtggccccc acgaagttt 
      gcctacgag cctggagat gagctccag atcacctgc 
      agtccgggg tttgaggcc aggttggag gcaaggcct 
      aagtgtctc ccagatggg aggtggtcg gccccctta 
      cccaactgc accaattat tcggagatt taa
    mRNA sequence
    • >TC003245 RA
      gtttacatc agttcgtca caaactctc ccagagaaa 
      gctgcgcta aatcacgca cgaaacaca atgctacaa 
      taatacgaa atgtttgcg gtaaacaaa tcagtgctt 
      atcgctgta cttttacac agttgtttc gccacaatt 
      tacgcaaga gtaatacaa gaagaaaca tcttcggat 
      gcgtgtcca cctcctcaa ttagccttc aacggtaca 
      gaaaccgag cagggagaa gttcgaatt cgcaaggat 
      tacacgcac attcgcgaa gtgaaattt tccggttca 
      attggcccc cttggtgaa aagcgtctt tgcaaaatc 
      aaatgtatc ggaggtcaa tgggtgggc cctctctgt 
      gtcgataat cacgataat ggcaggttt catccgctt 
      ttcaggagc tgtaagctg gagtacatc aatccgcat 
      ctggtggtt accttcagg aatgtctcc attcaaacc 
      gccggatgg gtgtttccc cacgaggcc aaacttcag 
      ataagatgt cgtgagctg gggctgtac aagctcttg 
      gggacggcg tcaccacgc tgtcaaaac ggcgtttgg 
      tcggcgaga ctgccgtca tgtgtgccc acaacgctc 
      ctcaccaat ttcacagag gacgctccg ccgactctc 
      ctaatcagg ataccatcg ggctcggcg tccgtcgaa 
      cccggaggg gaattggcg gtgtttccc ggatcgact 
      gtgcacctg gaatgtctc ttttcccgg cgacttggt 
      tcaccagat tggacgtgg acgtctccc ctgggacaa 
      tatctcacc ggttgggca atagcggca gaagagcgt 
      gattggaag tatagatta tcgatttat tatgtgaag 
      ccccaagac tccggtacg ttcacttgc gccactcct 
      agaggaatc accaacagt ctcactctg caagttgca 
      gccgttcat tgtgacccc atctctgtg tcgggcccc 
      catctcacc ataagggtg gaaggaacc cgtcttgga 
      cacactgcc atcttccaa tgtccaatg ggattcaaa 
      gttaatggt gcagctaat ctcacctgt caggcttcg 
      ggcaaatgg tcagcccct gttccaaca tgcgagctc 
      gtaaaatgc gaatcatta gacaccccc ggcggcctc 
      tacgagccc catttgcac ctcgaagag cacaacaac 
      agttacgga ggccgtgct atcttcagc tgtgcctgg 
      ggctaccgc ctcatgggg cccccaggc atcgaatgc 
      gaactgaac ggtaactgg tcggggcct ttgcccaaa 
      tgcgtccct attcaatgc ccgcccccg gtgattccc 
      gtcaacggc cacctcatc cagtcggag gcggcgggc 
      atggacggg ggccgctat gccgtgggc agtttagta 
      cagtttgcc tgcaggggg gcccaccag ctggagggc 
      gaagccagc attatttgt accgagact gggttttgg 
      tcgcacccg ccccctttc tgcaaaccg cggtgcccc 
      tatttgggg gagcccgac aatgggctg gtggccccc 
      acgaagttt gcctacgag cctggagat gagctccag 
      atcacctgc agtccgggg tttgaggcc aggttggag 
      gcaaggcct aagtgtctc ccagatggg aggtggtcg 
      gccccctta cccaactgc accaattat tcggagatt 
      taataagat attaagcgt tgaatgttt gtgataatg 
      atgatggga gaaacatcg ttgatttta tgactcgta 
      attaagtat ttttcggtt attttttac caaaaaagt 
      tgttacaca tgatgatct catgccgaa tacaaattt 
      ttatcaaat tgtagatat ttctagcga tagtgaatg 
      tatttacaa cgaaattta tgtaccatg taattgatt 
      ggtttttta tgtaaataa attcaaaaa ctgcct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY