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Gene information
General information
TC002427 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28918647..28925474
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002427
    iBeetle screen iB_06148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002427 RA
      MSSNNFDEV LSSLQDAQD SAKLLKILI DVRKKVDAD 
      CPKNKENIC KIREKGCFK QIVASLQTS KKSTINVSL 
      SILGNCCLD AKCARDVIS QYGALSNFN QLLKRYPKD 
      DSINGRVFR IVGNLCQHR DQWAGVIIE KKPQIVIHI 
      VKYLKDSVE EENGKVSEA TITMGIRAL RVLVNRETL 
      QSLVTTYGV LKTVGMLLI KYGAIWQET KKGESVLIN 
      IVKLLHEYS RYRYYPSIQ EMRSTDKGD SIVYLSKIL 
      LVAPRQVVK IVMNFLKIS QLKSDLPVP EIFDSFIEV 
      LSDDTIIQE FNSTCEECI KCLCYLLDH PGNRENERC 
      GDSIPLLIN VLSGLTLQT NNKIECSIL IISTLKKCK 
      YNFNLIADQ VRCNIVTVL NEKIKLIVG SVDPLNINH 
      KSDKKRRNN FLAKFKESK RSKLMVDLN KEDEESSSS 
      DSDEEIDKY YSSVRNDYS PGSSNESEM AMSWTSGLS 
      PPSSPRSRL YGISESDSD DYSPVCSEV DPSDFPLAK 
      PEGEESPNP EIDSKSSDI SEETEEIQS FDVDSSSVK 
      QIKGRLVLE IVDLLKTYI KIKPPITQL GTPELLLGL 
      IKSTAYFHW PCSSAILDI VDLIYKILG SHNYLLPLM 
      QSDFIPAVY DMAKIQHGS WCSKCRQFQ WISASIIKK 
      LSRVAETGG GKGDIAHKL LRGDNVTKQ QLVLVIPYI 
      IRSKAILVK LMLNCGGLE ILMKLLKDQ SALQTKSIK 
      VLCVLASKK LGIPNPKNV SGIYKNVTG GNTGDTSSV 
      VTFKLDDGS LLSADRGLL SRNSDFFAR LLNGDFKES 
      SQDVITLPN VNNKSLRCL LHWIKTDLV EIDIDLNTH 
      LDVIGLCDR FLMEDYRCY LTDCVEKFR LSPDTIPTI 
      YNWSLESRT NLLRVECIA YALVANIID SERFEMFRN 
      LFDLGYTEE LVEDIRKLL VRYMTLNQF CDCKKHKNR 
      NCDKKIHSI Y
    CDS sequence
    • >TC002427 RA
      atgtcaagt aataatttt gacgaggtt ttatcgtcc 
      cttcaagac gcccaagac agcgcaaaa ttactcaag 
      attctcatc gatgtacga aaaaaggta gacgccgac 
      tgccccaaa aacaaggag aacatttgc aaaatccgg 
      gaaaagggc tgctttaag caaatcgta gcctccctc 
      caaacctcc aagaagagc acgattaac gtctccttg 
      agcattttg gggaactgt tgtcttgac gcaaagtgt 
      gccagggat gtgatcagt caatatggc gctttgtcc 
      aatttcaat caacttttg aagcgctac ccgaaagat 
      gatagcatc aacgggcgc gtttttcga attgtgggc 
      aatttgtgc caacacagg gaccaatgg gcgggggtt 
      ataatcgag aaaaaaccg caaattgtt atacacatt 
      gtcaagtat cttaaagat tcggtggag gaagaaaat 
      gggaaagtt tccgaagct actattaca atgggtatt 
      cgggctttg agggtctta gttaatcga gaaacgctt 
      cagtcgctc gtgacaacg tacggggtg cttaaaact 
      gtagggatg ttgcttatt aagtatggt gccatctgg 
      caagagacg aaaaaaggc gaaagcgtg cttattaac 
      atcgttaag ttactccac gagtattcg cgctatcgc 
      tattatccc tctatccaa gaaatgaga agtacagac 
      aaaggcgac tcgattgtc taccttagc aaaattctc 
      ttagtggct ccgcgacaa gtcgttaaa attgtcatg 
      aacttcttg aaaatcagt cagttgaag tcagatttg 
      cccgttccg gagattttt gacagcttc attgaagtc 
      ttaagcgac gatacgatc attcaagag tttaattcc 
      acatgtgaa gagtgtatc aaatgtctt tgctacctt 
      ttggaccat ccaggaaat agagaaaat gaaaggtgt 
      ggcgacagc attccactt ttaattaat gtactaagt 
      gggctcacc cttcaaact aataataaa atcgagtgt 
      agcatttta attatatca actttgaag aagtgcaag 
      tacaacttt aacctgatc gcggatcag gtcaggtgt 
      aacattgtg acagtcttg aatgagaaa ataaaactg 
      atagtcggg agcgttgat ccgcttaat attaatcat 
      aaaagtgat aaaaaacgc agaaataat tttcttgcc 
      aagtttaag gaaagtaag aggagtaag ctaatggtg 
      gatttgaat aaggaagat gaagaaagt tctagttca 
      gatagcgac gaagaaatc gataagtat tattcaagt 
      gtcagaaat gactacagt cctgggtca agcaacgaa 
      tctgagatg gccatgtct tggacttca gggctgtcg 
      ccaccttca agccctcgg tcccgactt tatggtatt 
      tctgaatcc gattcggac gactattcg ccagtgtgt 
      agcgaagtg gacccttct gacttccca ttggcaaaa 
      ccagaaggt gaagaatcc ccgaatccc gaaatagat 
      tcgaaaagt tcggacatt tcggaagaa accgaagaa 
      attcaaagt ttcgacgtg gactcaagt agcgtcaaa 
      caaatcaaa ggacgcctc gttttagaa atagtcgat 
      ttgctcaaa acctacatc aaaatcaag ccaccgatt 
      acgcagttg ggaaccccc gaactactc ctcggccta 
      atcaaaagc acggcctac ttccactgg ccatgttca 
      tccgccatt ttggacata gttgatttg atttacaag 
      atattaggg tcacacaat tatctacta cctttgatg 
      caaagcgat tttattccg gctgtttac gacatggcc 
      aaaatccaa catggctcc tggtgttca aaatgtcgc 
      cagttccaa tggattagt gcttctata atcaaaaaa 
      ttatcacgg gtggctgag actggaggg gggaagggc 
      gatattgcc cataaacta ctccgaggg gataatgtc 
      actaaacaa cagttggta ttagtgatc ccatacatc 
      atcaggagc aaagcaatc ctggtgaag ctcatgctg 
      aactgcggc ggcctggag atcctgatg aagctcctc 
      aaggaccaa tctgccctc cagaccaaa agcatcaaa 
      gtcctttgt gtgttagca tcgaaaaaa cttggcatt 
      ccaaaccca aaaaacgtg tctggcatt tacaaaaac 
      gtgacgggt ggcaacact ggcgataca tcatccgtg 
      gtcacattc aaactagac gatgggagt ttactgagt 
      gccgacagg ggtttactg agccgtaat tcagatttt 
      tttgcccgc ttacttaac ggtgatttc aaagagtca 
      agccaggat gtgatcacg ttaccaaac gtcaacaat 
      aaatcctta aggtgtttg ctgcactgg atcaagacc 
      gacttggtt gaaattgat attgatttg aatactcat 
      ttggacgtt atcggacta tgtgatagg tttttgatg 
      gaggattat cggtgttat ttgaccgat tgtgtggaa 
      aagtttaga ttaagtcct gatacaatt ccgacgatt 
      tataattgg tcgcttgaa tcacggact aatcttcta 
      agggtcgag tgtattgct tatgccctt gttgccaac 
      attatagac tcagagagg tttgaaatg tttaggaat 
      ttgttcgat ttagggtat actgaagaa ttggttgag 
      gacatacgc aaattattg gtgaggtac atgaccttg 
      aaccaattc tgcgactgc aaaaagcac aaaaacaga 
      aactgtgat aaaaagatt catagtatt tattaa
    mRNA sequence
    • >TC002427 RA
      cgttaacat gacatgaca tgtgttaaa aaaatgtaa 
      ttttgacaa ataatcatt gaataagac ataatttat 
      gcccacatg cttacgata aaataatag tatattgca 
      aaaattgtc aaccatgtg gcataaaag cctcagtat 
      tgcccttat cttaataac tattacaga aatcagttt 
      ggcgaagat aatttgcta cgaatgttg gacaccatc 
      agaaagtta tgtccccat ctggccccc acaatccaa 
      ttggacgct ttgtgaaaa accctggag actgagaga 
      atgcattgg aaatttttc agcagacat gtgtttcag 
      ttttaactt caaaaagcg tacgttttc tatcattta 
      aatcgttct gaactctgt ggagtttca gtagtgttt 
      aaactattt ctcaacatt ttcagaggg tgtccaagc 
      caaaggatg cgccacttt cgacatggt cgcttacaa 
      aatagtctg ccgctacaa aatgaacaa aaagggttt 
      ccactgcaa cagctgcaa ctcgcattt gtgcaatag 
      tgcaaaccc cctaactcc aactccctt aattacaat 
      ttttgtcgt gttacttac ttattttaa gaataaaat 
      ttgaaccca cctgcctcg ttattgatc cggtacatg 
      tgtttatga aactcgggc ccgtgccca tccctgtct 
      ctattatta tgggttaca aataaataa gcgtgaatc 
      atttcatga agaagagtc tcaactaag tctttcctg 
      ggccttaat ttaagtaac ggttcactc aaggtgata 
      ctacaaagc ccccctctg ccactatac gagcaaatg 
      ccagcacac gaagtcatt ttcttgctc caagagaca 
      caaaccgcc accaattta ttccaaaaa aaacgttgg 
      tcaaaagct atgaacagg ttgtgtatg tcgggggtg 
      gggtccacg agctcccat gaggggtct atgagtgat 
      ttggtcgag tcggacccc tttttaggc tggtgatag 
      acaagacta gaagggggc tgttcaact gcagcctcc 
      tgttcgaac tgttcttgc aattgtttg gccaactca 
      gaatcagac tgttcggtt tgcccagct tcttgttca 
      aacctgtgc tgcaaaatg agcgccatt tggtagtcc 
      agctccatc ctaacctca aaaactaaa aaatcgaat 
      tagttgaaa cggtggaac ctacactac tagtgcgac 
      acaaaccgg cccctgatc gttgtcttg aggcgtttt 
      agctgtaaa attgtgatt tttgaccca attatgtcg 
      cactctatt ttcttggta cttcagtgg tcgcaaacg 
      tcattcgag tgacatgtc gggtgtttg gagccagat 
      tttgttgca aaaatcgcc gaaaaaaac acccagtcc 
      agtgtaatg aactaaagg cggcccaac aatgtcaag 
      taataattt tgacgaggt tttatcgtc ccttcaaga 
      cgcccaaga cagcgcaaa attactcaa gattctcat 
      cgatgtacg aaaaaaggt agacgccga ctgccccaa 
      aaacaagga gaacatttg caaaatccg ggaaaaggg 
      ctgctttaa gcaaatcgt agcctccct ccaaacctc 
      caagaagag cacgattaa cgtctcctt gagcatttt 
      ggggaactg ttgtcttga cgcaaagtg tgccaggga 
      tgtgatcag tcaatatgg cgctttgtc caatttcaa 
      tcaactttt gaagcgcta cccgaaaga tgatagcat 
      caacgggcg cgtttttcg aattgtggg caatttgtg 
      ccaacacag ggaccaatg ggcgggggt tataatcga 
      gaaaaaacc gcaaattgt tatacacat tgtcaagta 
      tcttaaaga ttcggtgga ggaagaaaa tgggaaagt 
      ttccgaagc tactattac aatgggtat tcgggcttt 
      gagggtctt agttaatcg agaaacgct tcagtcgct 
      cgtgacaac gtacggggt gcttaaaac tgtagggat 
      gttgcttat taagtatgg tgccatctg gcaagagac 
      gaaaaaagg cgaaagcgt gcttattaa catcgttaa 
      gttactcca cgagtattc gcgctatcg ctattatcc 
      ctctatcca agaaatgag aagtacaga caaaggcga 
      ctcgattgt ctaccttag caaaattct cttagtggc 
      tccgcgaca agtcgttaa aattgtcat gaacttctt 
      gaaaatcag tcagttgaa gtcagattt gcccgttcc 
      ggagatttt tgacagctt cattgaagt cttaagcga 
      cgatacgat cattcaaga gtttaattc cacatgtga 
      agagtgtat caaatgtct ttgctacct tttggacca 
      tccaggaaa tagagaaaa tgaaaggtg tggcgacag 
      cattccact tttaattaa tgtactaag tgggctcac 
      ccttcaaac taataataa aatcgagtg tagcatttt 
      aattatatc aactttgaa gaagtgcaa gtacaactt 
      taacctgat cgcggatca ggtcaggtg taacattgt 
      gacagtctt gaatgagaa aataaaact gatagtcgg 
      gagcgttga tccgcttaa tattaatca taaaagtga 
      taaaaaacg cagaaataa ttttcttgc caagtttaa 
      ggaaagtaa gaggagtaa gctaatggt ggatttgaa 
      taaggaaga tgaagaaag ttctagttc agatagcga 
      cgaagaaat cgataagta ttattcaag tgtcagaaa 
      tgactacag tcctgggtc aagcaacga atctgagat 
      ggccatgtc ttggacttc agggctgtc gccaccttc 
      aagccctcg gtcccgact ttatggtat ttctgaatc 
      cgattcgga cgactattc gccagtgtg tagcgaagt 
      ggacccttc tgacttccc attggcaaa accagaagg 
      tgaagaatc cccgaatcc cgaaataga ttcgaaaag 
      ttcggacat ttcggaaga aaccgaaga aattcaaag 
      tttcgacgt ggactcaag tagcgtcaa acaaatcaa 
      aggacgcct cgttttaga aatagtcga tttgctcaa 
      aacctacat caaaatcaa gccaccgat tacgcagtt 
      gggaacccc cgaactact cctcggcct aatcaaaag 
      cacggccta cttccactg gccatgttc atccgccat 
      tttggacat agttgattt gatttacaa gatattagg 
      gtcacacaa ttatctact acctttgat gcaaagcga 
      ttttattcc ggctgttta cgacatggc caaaatcca 
      acatggctc ctggtgttc aaaatgtcg ccagttcca 
      atggattag tgcttctat aatcaaaaa attatcacg 
      ggtggctga gactggagg ggggaaggg cgatattgc 
      ccataaact actccgagg ggataatgt cactaaaca 
      acagttggt attagtgat cccatacat catcaggag 
      caaagcaat cctggtgaa gctcatgct gaactgcgg 
      cggcctgga gatcctgat gaagctcct caaggacca 
      atctgccct ccagaccaa aagcatcaa agtcctttg 
      tgtgttagc atcgaaaaa acttggcat tccaaaccc 
      aaaaaacgt gtctggcat ttacaaaaa cgtgacggg 
      tggcaacac tggcgatac atcatccgt ggtcacatt 
      caaactaga cgatgggag tttactgag tgccgacag 
      gggtttact gagccgtaa ttcagattt ttttgcccg 
      cttacttaa cggtgattt caaagagtc aagccagga 
      tgtgatcac gttaccaaa cgtcaacaa taaatcctt 
      aaggtgttt gctgcactg gatcaagac cgacttggt 
      tgaaattga tattgattt gaatactca tttggacgt 
      tatcggact atgtgatag gtttttgat ggaggatta 
      tcggtgtta tttgaccga ttgtgtgga aaagtttag 
      attaagtcc tgatacaat tccgacgat ttataattg 
      gtcgcttga atcacggac taatcttct aagggtcga 
      gtgtattgc ttatgccct tgttgccaa cattataga 
      ctcagagag gtttgaaat gtttaggaa tttgttcga 
      tttagggta tactgaaga attggttga ggacatacg 
      caaattatt ggtgaggta catgacctt gaaccaatt 
      ctgcgactg caaaaagca caaaaacag aaactgtga 
      taaaaagat tcatagtat ttattaagt agtaggttt 
      tttacgtgg cagttaccc aaataaact tttttctag 
      taacagttg actgcaaca ataatttac aatatgtac 
      aacaagtag tgatcaaga gtggctgga gaatcagta 
      ctgccaact gataccgtc actcttgat cacgctgta 
      gcacgactt tgttataaa aataaatca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY