| General information | |||
| TC033926
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 873115..878100 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033926 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06146 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC033926 RA
MGKRTCFLC RVETKPLFE FPSDFETRA KWFKAIEHL CDTSTITDY SKLCFNHFE ESCFRRGET RAFLIRKAV PTIFDGPNM PWHHDNIDM IKCCRETDK ICQDSEKDP SSVEPVFLK TNELLDLQG DQDENDSTL SNDFLEKPY FRSVKTFMQ GSRAVSDVC RICFRSSSN EDAIKLYEK TLKHDVAYI ENMLKSVLP NLDLNVYAH NTICARCFE FLDYSYRLK NDWLLTEQK LQKILKTKR RKLNCMVDV VILMEDEEQ NVANVAHDH SYDLKVENQ MQSEKDIIE EDIISEDEK STKKEGKRL RKKEGKHSS WGLGPYMCE ICGCTYTHL KSFQRHIRL KNAKML
- >TC033926 RB
MGKRTCFLC RVETKPLFE FPSDFETRA KWFKAIEHL CDTSTITDY SKLCFNHFE ESCFRRDEL LDLQGDQDE NDSTLSNDF LEKPYFRSV KTFMQGSRA VSDVCRICF RSSSNEDAI KLYEKTLKH DVAYIENML KSVLPNLDL NVYAHNTIC ARCFEFLDY SYRLKNDWL LTEQKLQKI LKTKRRKLN CMVDVVILM EDEEQNVAN VAHDHSYDL KVENQMQSE KDIIEEDII SEDEKSTKK EGKRLRKKE GKHSSWGLG PYMCEICGC TYTHLKSFQ RHIRLKNAK ML
- >TC033926 RA
atggggaag agaacatgt tttctttgt cgtgtagaa acaaaacct ttgttcgaa tttccatcc gatttcgaa acacgcgca aaatggttt aaagctatt gaacacctc tgtgatacc tcaacaata acagattat tcaaaatta tgtttcaat cattttgaa gaaagttgt ttcaggaga ggtgagacg agagctttt ctaattagg aaagccgtt cccacaatt tttgatgga ccaaacatg ccttggcac catgacaat attgacatg ataaaatgt tgcagagaa acagacaaa atttgtcag gattctgag aaagatcca agttccgtg gaacccgtt tttttgaaa acaaacgaa ttattggac ctgcaagga gatcaagat gaaaacgat agcacactt tcaaatgat tttctagaa aagccttat ttcagatca gtcaaaact tttatgcaa ggttctcgg gcagtttcc gatgtatgt cggatttgt ttccgttct tcgagtaac gaagatgct atcaaattg tatgaaaaa acattgaag catgatgtc gcttatatc gaaaatatg ttaaagtca gtgctacct aatctagac ctgaatgtt tatgcccat aacacgatt tgtgcaagg tgtttcgag tttttagat tacagttat cgtttgaaa aacgactgg ttattgacg gaacaaaaa ttacaaaaa atactaaaa acaaagaga cgaaaattg aattgtatg gtagacgta gttattcta atggaagat gaagagcaa aacgttgca aatgttgct catgatcac tcttacgat ctaaaagtt gagaatcaa atgcaatct gaaaaagac ataattgaa gaggacata atttccgag gatgaaaaa tcaacaaaa aaagaaggg aaacgatta agaaaaaaa gaagggaaa cattcatcg tggggttta ggcccttac atgtgtgaa atctgcggt tgtacatac acacatctg aaatcattt caacggcat atcagactc aaaaatgcc aaaatgttg taa
- >TC033926 RB
atggggaag agaacatgt tttctttgt cgtgtagaa acaaaacct ttgttcgaa tttccatcc gatttcgaa acacgcgca aaatggttt aaagctatt gaacacctc tgtgatacc tcaacaata acagattat tcaaaatta tgtttcaat cattttgaa gaaagttgt ttcaggaga gacgaatta ttggacctg caaggagat caagatgaa aacgatagc acactttca aatgatttt ctagaaaag ccttatttc agatcagtc aaaactttt atgcaaggt tctcgggca gtttccgat gtatgtcgg atttgtttc cgttcttcg agtaacgaa gatgctatc aaattgtat gaaaaaaca ttgaagcat gatgtcgct tatatcgaa aatatgtta aagtcagtg ctacctaat ctagacctg aatgtttat gcccataac acgatttgt gcaaggtgt ttcgagttt ttagattac agttatcgt ttgaaaaac gactggtta ttgacggaa caaaaatta caaaaaata ctaaaaaca aagagacga aaattgaat tgtatggta gacgtagtt attctaatg gaagatgaa gagcaaaac gttgcaaat gttgctcat gatcactct tacgatcta aaagttgag aatcaaatg caatctgaa aaagacata attgaagag gacataatt tccgaggat gaaaaatca acaaaaaaa gaagggaaa cgattaaga aaaaaagaa gggaaacat tcatcgtgg ggtttaggc ccttacatg tgtgaaatc tgcggttgt acatacaca catctgaaa tcatttcaa cggcatatc agactcaaa aatgccaaa atgttgtaa
- >TC033926 RA
tttcacatg tcacatttg ctcccaggt tggtgaaca gctgtcttt tgattttga aaagtattt atgttgggc gggttccgt gtgtttgtt taataacta aatgttcta cattcgggg ttattaaaa atcaagcaa gaaacgctc attttaaaa ttggtgtgc tttcattat ggggaagag aacatgttt tctttgtcg tgtagaaac aaaaccttt gttcgaatt tccatccga tttcgaaac acgcgcaaa atggtttaa agctattga acacctctg tgatacctc aacaataac agattattc aaaattatg tttcaatca ttttgaaga aagttgttt caggagagg tgagacgag agcttttct aattaggaa agccgttcc cacaatttt tgatggacc aaacatgcc ttggcacca tgacaatat tgacatgat aaaatgttg cagagaaac agacaaaat ttgtcagga ttctgagaa agatccaag ttccgtgga acccgtttt tttgaaaac aaacgaatt attggacct gcaaggaga tcaagatga aaacgatag cacactttc aaatgattt tctagaaaa gccttattt cagatcagt caaaacttt tatgcaagg ttctcgggc agtttccga tgtatgtcg gatttgttt ccgttcttc gagtaacga agatgctat caaattgta tgaaaaaac attgaagca tgatgtcgc ttatatcga aaatatgtt aaagtcagt gctacctaa tctagacct gaatgttta tgcccataa cacgatttg tgcaaggtg tttcgagtt tttagatta cagttatcg tttgaaaaa cgactggtt attgacgga acaaaaatt acaaaaaat actaaaaac aaagagacg aaaattgaa ttgtatggt agacgtagt tattctaat ggaagatga agagcaaaa cgttgcaaa tgttgctca tgatcactc ttacgatct aaaagttga gaatcaaat gcaatctga aaaagacat aattgaaga ggacataat ttccgagga tgaaaaatc aacaaaaaa agaagggaa acgattaag aaaaaaaga agggaaaca ttcatcgtg gggtttagg cccttacat gtgtgaaat ctgcggttg tacatacac acatctgaa atcatttca acggcatat cagactcaa aaatgccaa aatgttgta aaagaaaaa ttacctcct aaagaaaat caagggtta tgtgtgaac agtgcggtc atttatcac gcgatattc agtgtctca acaaacatt tacaaacac atggagaac atgtttgtg tcttgtgta aaaaacact tcgcgaata agcctttac tgcttagac atatttcta taactcatg tgggagtgt tgaattggc agtgtcact tctgtggga aaagatttg cttcgcgta aagttttga atgaacata tgttatatc ataacccaa agattcctt gtgaaacat gtggcaaaa aatttgcca cacgtgttg tcatgaaga tacatgctc gcacacacc ttaaaaagg tattcaatt gccagcagt gtgaaaaaa tattcaagt acaaaatct cactggata accatatga agacgttcc ataaaacac aacgaaacg ttgtttgcg aattttgtg gtaaagaat atactaaga aggttgaat taaggaaac acaagttat ggattcatg gttacattg accagaaaa gtgggaaaa caagttaat agttttgtg catattaat aaaaaaaaa taaaaaaca caagcaaag
- >TC033926 RB
tttcacatg tcacatttg ctcccaggt tggtgaaca gctgtcttt tgattttga aaagtattt atgttgggc gggttccgt gtgtttgtt taataacta aatgttcta cattcgggg ttattaaaa atcaagcaa gaaacgctc attttaaaa ttggtgtgc tttcattat ggggaagag aacatgttt tctttgtcg tgtagaaac aaaaccttt gttcgaatt tccatccga tttcgaaac acgcgcaaa atggtttaa agctattga acacctctg tgatacctc aacaataac agattattc aaaattatg tttcaatca ttttgaaga aagttgttt caggagaga cgaattatt ggacctgca aggagatca agatgaaaa cgatagcac actttcaaa tgattttct agaaaagcc ttatttcag atcagtcaa aacttttat gcaaggttc tcgggcagt ttccgatgt atgtcggat ttgtttccg ttcttcgag taacgaaga tgctatcaa attgtatga aaaaacatt gaagcatga tgtcgctta tatcgaaaa tatgttaaa gtcagtgct acctaatct agacctgaa tgtttatgc ccataacac gatttgtgc aaggtgttt cgagttttt agattacag ttatcgttt gaaaaacga ctggttatt gacggaaca aaaattaca aaaaatact aaaaacaaa gagacgaaa attgaattg tatggtaga cgtagttat tctaatgga agatgaaga gcaaaacgt tgcaaatgt tgctcatga tcactctta cgatctaaa agttgagaa tcaaatgca atctgaaaa agacataat tgaagagga cataatttc cgaggatga aaaatcaac aaaaaaaga agggaaacg attaagaaa aaaagaagg gaaacattc atcgtgggg tttaggccc ttacatgtg tgaaatctg cggttgtac atacacaca tctgaaatc atttcaacg gcatatcag actcaaaaa tgccaaaat gttgtaaaa gaaaaatta cctcctaaa gaaaatcaa gggttatgt gtgaacagt gcggtcatt tatcacgcg atattcagt gtctcaaca aacatttac aaacacatg gagaacatg tttgtgtct tgtgtaaaa aacacttcg cgaataagc ctttactgc ttagacata tttctataa ctcatgtgg gagtgttga attggcagt gtcacttct gtgggaaaa gatttgctt cgcgtaaag ttttgaatg aacatatgt tatatcata acccaaaga ttccttgtg aaacatgtg gcaaaaaat ttgccacac gtgttgtca tgaagatac atgctcgca cacacctta aaaaggtat tcaattgcc agcagtgtg aaaaaatat tcaagtaca aaatctcac tggataacc atatgaaga cgttccata aaacacaac gaaacgttg tttgcgaat tttgtggta aagaatata ctaagaagg ttgaattaa ggaaacaca agttatgga ttcatggtt acattgacc agaaaagtg ggaaaacaa gttaatagt tttgtgcat attaataaa aaaaaataa aaaacacaa gcaaag
