Status
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Gene information
General information
TC033926
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002213
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 873115..878100
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033926
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06146

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033926 RA
      MGKRTCFLC RVETKPLFE FPSDFETRA KWFKAIEHL 
      CDTSTITDY SKLCFNHFE ESCFRRGET RAFLIRKAV 
      PTIFDGPNM PWHHDNIDM IKCCRETDK ICQDSEKDP 
      SSVEPVFLK TNELLDLQG DQDENDSTL SNDFLEKPY 
      FRSVKTFMQ GSRAVSDVC RICFRSSSN EDAIKLYEK 
      TLKHDVAYI ENMLKSVLP NLDLNVYAH NTICARCFE 
      FLDYSYRLK NDWLLTEQK LQKILKTKR RKLNCMVDV 
      VILMEDEEQ NVANVAHDH SYDLKVENQ MQSEKDIIE 
      EDIISEDEK STKKEGKRL RKKEGKHSS WGLGPYMCE 
      ICGCTYTHL KSFQRHIRL KNAKML
    • >TC033926 RB
      MGKRTCFLC RVETKPLFE FPSDFETRA KWFKAIEHL 
      CDTSTITDY SKLCFNHFE ESCFRRDEL LDLQGDQDE 
      NDSTLSNDF LEKPYFRSV KTFMQGSRA VSDVCRICF 
      RSSSNEDAI KLYEKTLKH DVAYIENML KSVLPNLDL 
      NVYAHNTIC ARCFEFLDY SYRLKNDWL LTEQKLQKI 
      LKTKRRKLN CMVDVVILM EDEEQNVAN VAHDHSYDL 
      KVENQMQSE KDIIEEDII SEDEKSTKK EGKRLRKKE 
      GKHSSWGLG PYMCEICGC TYTHLKSFQ RHIRLKNAK 
      ML
    CDS sequence
    • >TC033926 RA
      atggggaag agaacatgt tttctttgt cgtgtagaa 
      acaaaacct ttgttcgaa tttccatcc gatttcgaa 
      acacgcgca aaatggttt aaagctatt gaacacctc 
      tgtgatacc tcaacaata acagattat tcaaaatta 
      tgtttcaat cattttgaa gaaagttgt ttcaggaga 
      ggtgagacg agagctttt ctaattagg aaagccgtt 
      cccacaatt tttgatgga ccaaacatg ccttggcac 
      catgacaat attgacatg ataaaatgt tgcagagaa 
      acagacaaa atttgtcag gattctgag aaagatcca 
      agttccgtg gaacccgtt tttttgaaa acaaacgaa 
      ttattggac ctgcaagga gatcaagat gaaaacgat 
      agcacactt tcaaatgat tttctagaa aagccttat 
      ttcagatca gtcaaaact tttatgcaa ggttctcgg 
      gcagtttcc gatgtatgt cggatttgt ttccgttct 
      tcgagtaac gaagatgct atcaaattg tatgaaaaa 
      acattgaag catgatgtc gcttatatc gaaaatatg 
      ttaaagtca gtgctacct aatctagac ctgaatgtt 
      tatgcccat aacacgatt tgtgcaagg tgtttcgag 
      tttttagat tacagttat cgtttgaaa aacgactgg 
      ttattgacg gaacaaaaa ttacaaaaa atactaaaa 
      acaaagaga cgaaaattg aattgtatg gtagacgta 
      gttattcta atggaagat gaagagcaa aacgttgca 
      aatgttgct catgatcac tcttacgat ctaaaagtt 
      gagaatcaa atgcaatct gaaaaagac ataattgaa 
      gaggacata atttccgag gatgaaaaa tcaacaaaa 
      aaagaaggg aaacgatta agaaaaaaa gaagggaaa 
      cattcatcg tggggttta ggcccttac atgtgtgaa 
      atctgcggt tgtacatac acacatctg aaatcattt 
      caacggcat atcagactc aaaaatgcc aaaatgttg 
      taa
    • >TC033926 RB
      atggggaag agaacatgt tttctttgt cgtgtagaa 
      acaaaacct ttgttcgaa tttccatcc gatttcgaa 
      acacgcgca aaatggttt aaagctatt gaacacctc 
      tgtgatacc tcaacaata acagattat tcaaaatta 
      tgtttcaat cattttgaa gaaagttgt ttcaggaga 
      gacgaatta ttggacctg caaggagat caagatgaa 
      aacgatagc acactttca aatgatttt ctagaaaag 
      ccttatttc agatcagtc aaaactttt atgcaaggt 
      tctcgggca gtttccgat gtatgtcgg atttgtttc 
      cgttcttcg agtaacgaa gatgctatc aaattgtat 
      gaaaaaaca ttgaagcat gatgtcgct tatatcgaa 
      aatatgtta aagtcagtg ctacctaat ctagacctg 
      aatgtttat gcccataac acgatttgt gcaaggtgt 
      ttcgagttt ttagattac agttatcgt ttgaaaaac 
      gactggtta ttgacggaa caaaaatta caaaaaata 
      ctaaaaaca aagagacga aaattgaat tgtatggta 
      gacgtagtt attctaatg gaagatgaa gagcaaaac 
      gttgcaaat gttgctcat gatcactct tacgatcta 
      aaagttgag aatcaaatg caatctgaa aaagacata 
      attgaagag gacataatt tccgaggat gaaaaatca 
      acaaaaaaa gaagggaaa cgattaaga aaaaaagaa 
      gggaaacat tcatcgtgg ggtttaggc ccttacatg 
      tgtgaaatc tgcggttgt acatacaca catctgaaa 
      tcatttcaa cggcatatc agactcaaa aatgccaaa 
      atgttgtaa
    mRNA sequence
    • >TC033926 RA
      tttcacatg tcacatttg ctcccaggt tggtgaaca 
      gctgtcttt tgattttga aaagtattt atgttgggc 
      gggttccgt gtgtttgtt taataacta aatgttcta 
      cattcgggg ttattaaaa atcaagcaa gaaacgctc 
      attttaaaa ttggtgtgc tttcattat ggggaagag 
      aacatgttt tctttgtcg tgtagaaac aaaaccttt 
      gttcgaatt tccatccga tttcgaaac acgcgcaaa 
      atggtttaa agctattga acacctctg tgatacctc 
      aacaataac agattattc aaaattatg tttcaatca 
      ttttgaaga aagttgttt caggagagg tgagacgag 
      agcttttct aattaggaa agccgttcc cacaatttt 
      tgatggacc aaacatgcc ttggcacca tgacaatat 
      tgacatgat aaaatgttg cagagaaac agacaaaat 
      ttgtcagga ttctgagaa agatccaag ttccgtgga 
      acccgtttt tttgaaaac aaacgaatt attggacct 
      gcaaggaga tcaagatga aaacgatag cacactttc 
      aaatgattt tctagaaaa gccttattt cagatcagt 
      caaaacttt tatgcaagg ttctcgggc agtttccga 
      tgtatgtcg gatttgttt ccgttcttc gagtaacga 
      agatgctat caaattgta tgaaaaaac attgaagca 
      tgatgtcgc ttatatcga aaatatgtt aaagtcagt 
      gctacctaa tctagacct gaatgttta tgcccataa 
      cacgatttg tgcaaggtg tttcgagtt tttagatta 
      cagttatcg tttgaaaaa cgactggtt attgacgga 
      acaaaaatt acaaaaaat actaaaaac aaagagacg 
      aaaattgaa ttgtatggt agacgtagt tattctaat 
      ggaagatga agagcaaaa cgttgcaaa tgttgctca 
      tgatcactc ttacgatct aaaagttga gaatcaaat 
      gcaatctga aaaagacat aattgaaga ggacataat 
      ttccgagga tgaaaaatc aacaaaaaa agaagggaa 
      acgattaag aaaaaaaga agggaaaca ttcatcgtg 
      gggtttagg cccttacat gtgtgaaat ctgcggttg 
      tacatacac acatctgaa atcatttca acggcatat 
      cagactcaa aaatgccaa aatgttgta aaagaaaaa 
      ttacctcct aaagaaaat caagggtta tgtgtgaac 
      agtgcggtc atttatcac gcgatattc agtgtctca 
      acaaacatt tacaaacac atggagaac atgtttgtg 
      tcttgtgta aaaaacact tcgcgaata agcctttac 
      tgcttagac atatttcta taactcatg tgggagtgt 
      tgaattggc agtgtcact tctgtggga aaagatttg 
      cttcgcgta aagttttga atgaacata tgttatatc 
      ataacccaa agattcctt gtgaaacat gtggcaaaa 
      aatttgcca cacgtgttg tcatgaaga tacatgctc 
      gcacacacc ttaaaaagg tattcaatt gccagcagt 
      gtgaaaaaa tattcaagt acaaaatct cactggata 
      accatatga agacgttcc ataaaacac aacgaaacg 
      ttgtttgcg aattttgtg gtaaagaat atactaaga 
      aggttgaat taaggaaac acaagttat ggattcatg 
      gttacattg accagaaaa gtgggaaaa caagttaat 
      agttttgtg catattaat aaaaaaaaa taaaaaaca 
      caagcaaag
    • >TC033926 RB
      tttcacatg tcacatttg ctcccaggt tggtgaaca 
      gctgtcttt tgattttga aaagtattt atgttgggc 
      gggttccgt gtgtttgtt taataacta aatgttcta 
      cattcgggg ttattaaaa atcaagcaa gaaacgctc 
      attttaaaa ttggtgtgc tttcattat ggggaagag 
      aacatgttt tctttgtcg tgtagaaac aaaaccttt 
      gttcgaatt tccatccga tttcgaaac acgcgcaaa 
      atggtttaa agctattga acacctctg tgatacctc 
      aacaataac agattattc aaaattatg tttcaatca 
      ttttgaaga aagttgttt caggagaga cgaattatt 
      ggacctgca aggagatca agatgaaaa cgatagcac 
      actttcaaa tgattttct agaaaagcc ttatttcag 
      atcagtcaa aacttttat gcaaggttc tcgggcagt 
      ttccgatgt atgtcggat ttgtttccg ttcttcgag 
      taacgaaga tgctatcaa attgtatga aaaaacatt 
      gaagcatga tgtcgctta tatcgaaaa tatgttaaa 
      gtcagtgct acctaatct agacctgaa tgtttatgc 
      ccataacac gatttgtgc aaggtgttt cgagttttt 
      agattacag ttatcgttt gaaaaacga ctggttatt 
      gacggaaca aaaattaca aaaaatact aaaaacaaa 
      gagacgaaa attgaattg tatggtaga cgtagttat 
      tctaatgga agatgaaga gcaaaacgt tgcaaatgt 
      tgctcatga tcactctta cgatctaaa agttgagaa 
      tcaaatgca atctgaaaa agacataat tgaagagga 
      cataatttc cgaggatga aaaatcaac aaaaaaaga 
      agggaaacg attaagaaa aaaagaagg gaaacattc 
      atcgtgggg tttaggccc ttacatgtg tgaaatctg 
      cggttgtac atacacaca tctgaaatc atttcaacg 
      gcatatcag actcaaaaa tgccaaaat gttgtaaaa 
      gaaaaatta cctcctaaa gaaaatcaa gggttatgt 
      gtgaacagt gcggtcatt tatcacgcg atattcagt 
      gtctcaaca aacatttac aaacacatg gagaacatg 
      tttgtgtct tgtgtaaaa aacacttcg cgaataagc 
      ctttactgc ttagacata tttctataa ctcatgtgg 
      gagtgttga attggcagt gtcacttct gtgggaaaa 
      gatttgctt cgcgtaaag ttttgaatg aacatatgt 
      tatatcata acccaaaga ttccttgtg aaacatgtg 
      gcaaaaaat ttgccacac gtgttgtca tgaagatac 
      atgctcgca cacacctta aaaaggtat tcaattgcc 
      agcagtgtg aaaaaatat tcaagtaca aaatctcac 
      tggataacc atatgaaga cgttccata aaacacaac 
      gaaacgttg tttgcgaat tttgtggta aagaatata 
      ctaagaagg ttgaattaa ggaaacaca agttatgga 
      ttcatggtt acattgacc agaaaagtg ggaaaacaa 
      gttaatagt tttgtgcat attaataaa aaaaaataa 
      aaaacacaa gcaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY