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Gene information
General information
TC001704 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7870181..7873568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001704
    iBeetle screen iB_06141

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001704 RA
      MTESDKAEV FNFLLETGC CKRCALRYV GDNTADYDN 
      PQQNGHDQL AKKTKLNSC IICLDLLQD VTLESMVQC 
      EELEGVKEY DCKTFINFI SFPVAVLIR EHSMEIHMN 
      EKFPKFFED RNIVPINRA WSLSIQNKL GEKLGKKFN 
      KDSLLQIQF NTTYEFEKE ESSIMFLME SRLKKSNGQ 
      NEGLSRNVI GEFLKTINT ELFKKHAAV PPEPPGKAL 
      SFDQVAYKH DYIFIAGRY CKYSRNLFQ TPWANDEVQ 
      VINSSVQEI LFKSLESVF KCSSLVFSS SGREDFDVR 
      MLGRGRPFF VKICDPKVT TIPFEQFRL IEKQIEETN 
      LIEVRDLQF ISRKLLNNI KEGEQFKQK TYRALCVVD 
      KPEKIGEYI EKINNMKRV KLLQKTPLR VYHRRSADV 
      REKEIYNME ARPVTGASS LFELDVLTQ AGTYVKEFV 
      HGDLGRTRP SLREIMGGY VDIIALDVL DIHLDFPPR 
      INKKQK
    CDS sequence
    • >TC001704 RA
      atgaccgaa agtgataaa gccgaagtt ttcaatttt 
      ttattggag actgggtgt tgcaaaagg tgcgcttta 
      cgctacgtt ggcgataac acagccgat tatgataat 
      ccacagcaa aacggacac gaccagttg gcgaaaaag 
      acgaaattg aattcgtgc ataatttgt ctcgatttg 
      ctccaagat gtgactctt gaatccatg gttcagtgt 
      gaagaactg gagggtgtt aaggagtac gattgcaag 
      acttttatt aatttcatt agttttccg gttgctgtt 
      ctaataagg gaacacagc atggagatt catatgaat 
      gagaagttc cccaagttt tttgaagat cgtaacatt 
      gttccaata aacagggca tggtccttg tcaatacaa 
      aacaaactg ggcgaaaaa cttggcaaa aagttcaat 
      aaagattca ttgctccaa atccagttt aacacgact 
      tatgaattt gaaaaagag gagtcgtca attatgttt 
      ctgatggaa agtagactg aaaaaatca aatggacaa 
      aacgagggt cttagtcgt aacgtaatc ggggagttt 
      ttaaagaca ataaacacc gaacttttc aaaaaacat 
      gccgctgtc cctccggaa ccgcccggg aaagcttta 
      agtttcgac caagtggct tacaagcac gattatatt 
      tttatagcc ggaaggtac tgtaaatat tcaagaaac 
      ctgtttcaa acaccgtgg gccaacgac gaagtccaa 
      gtaatcaac agttcagtt caagaaatt ttatttaag 
      tcgctggaa agcgtcttt aaatgcagc agtttggtg 
      ttttcgtca agtggtcgt gaggatttt gacgtacga 
      atgctagga agaggccgt ccgtttttc gtaaaaata 
      tgtgacccc aaagtcacc acaatacca tttgaacaa 
      ttccgcctg attgagaaa caaattgaa gaaactaat 
      ttaattgaa gtcagagac ttgcaattt atttcgcgg 
      aagttgctt aacaatatc aaagagggg gaacaattt 
      aaacaaaag acgtacagg gcgttgtgt gtcgtagac 
      aagccggaa aaaattggc gaatatatt gaaaaaatc 
      aacaacatg aaaagggtg aaattgcta caaaaaacg 
      cccctgaga gtgtatcac aggcggtcg gctgacgtc 
      agggagaag gagatttac aacatggag gcaaggccg 
      gtcacaggt gctagtagt ttattcgag ttggacgtt 
      ctgacccaa gctggtact tacgtaaaa gagttcgtg 
      cacggcgac ctcggaaga actagacct tcattacgc 
      gaaataatg ggcggttac gtcgacatt atcgcttta 
      gatgtattg gacattcac ttagatttt ccaccccgt 
      ataaataaa aaacaaaaa taa
    mRNA sequence
    • >TC001704 RA
      actttttgc caccacatt attttattt aaaaatgac 
      cgaaagtga taaagccga agttttcaa ttttttatt 
      ggagactgg gtgttgcaa aaggtgcgc tttacgcta 
      cgttggcga taacacagc cgattatga taatccaca 
      gcaaaacgg acacgacca gttggcgaa aaagacgaa 
      attgaattc gtgcataat ttgtctcga tttgctcca 
      agatgtgac tcttgaatc catggttca gtgtgaaga 
      actggaggg tgttaagga gtacgattg caagacttt 
      tattaattt cattagttt tccggttgc tgttctaat 
      aagggaaca cagcatgga gattcatat gaatgagaa 
      gttccccaa gttttttga agatcgtaa cattgttcc 
      aataaacag ggcatggtc cttgtcaat acaaaacaa 
      actgggcga aaaacttgg caaaaagtt caataaaga 
      ttcattgct ccaaatcca gtttaacac gacttatga 
      atttgaaaa agaggagtc gtcaattat gtttctgat 
      ggaaagtag actgaaaaa atcaaatgg acaaaacga 
      gggtcttag tcgtaacgt aatcgggga gtttttaaa 
      gacaataaa caccgaact tttcaaaaa acatgccgc 
      tgtccctcc ggaaccgcc cgggaaagc tttaagttt 
      cgaccaagt ggcttacaa gcacgatta tatttttat 
      agccggaag gtactgtaa atattcaag aaacctgtt 
      tcaaacacc gtgggccaa cgacgaagt ccaagtaat 
      caacagttc agttcaaga aattttatt taagtcgct 
      ggaaagcgt ctttaaatg cagcagttt ggtgttttc 
      gtcaagtgg tcgtgagga ttttgacgt acgaatgct 
      aggaagagg ccgtccgtt tttcgtaaa aatatgtga 
      ccccaaagt caccacaat accatttga acaattccg 
      cctgattga gaaacaaat tgaagaaac taatttaat 
      tgaagtcag agacttgca atttatttc gcggaagtt 
      gcttaacaa tatcaaaga gggggaaca atttaaaca 
      aaagacgta cagggcgtt gtgtgtcgt agacaagcc 
      ggaaaaaat tggcgaata tattgaaaa aatcaacaa 
      catgaaaag ggtgaaatt gctacaaaa aacgcccct 
      gagagtgta tcacaggcg gtcggctga cgtcaggga 
      gaaggagat ttacaacat ggaggcaag gccggtcac 
      aggtgctag tagtttatt cgagttgga cgttctgac 
      ccaagctgg tacttacgt aaaagagtt cgtgcacgg 
      cgacctcgg aagaactag accttcatt acgcgaaat 
      aatgggcgg ttacgtcga cattatcgc tttagatgt 
      attggacat tcacttaga ttttccacc ccgtataaa 
      taaaaaaca aaaataaag tgtgtaatt catttattt 
      ggtaactta tacatgaaa caatact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY