Status
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Gene information
General information
TC001383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15262621..15265113
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001383
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06139

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001383 RA
      MSQFKFVTE MNNLVTMDG PLTKGPPPR WQRKGQENY 
      QSSNNLSLN SSKQKSFST SITKTPTKS EVKSKKTPT 
      KTPNKNRSN GSKTPTPNK TASKTPVAD RFIPVRSMS 
      NFDLAHYKL SQNEDTSDS PTQKELQKA MFENLHGAN 
      IDSQKILSY TNKPPTAPA GFQNPMRVI YTQTKTPAS 
      VKSNNRYIP QAPDRILDA PDIVDDYYL NLMDWSAGN 
      VLAVALGAH VYLWNAGTG NIEDLLELQ GNDYVCSLA 
      WIQDGDHLA VGTTNGTVE LWDCGRAKR LRVMDGHSA 
      RVGSLSWNS YVLTSGCRS GQIIHHDVR QREHIITTL 
      SGHTQEVCG LKWSPDGRY LASGGNDNV LNIWQSQTG 
      SHHTNNQPL HIFTAHQAA VKALAWCPW QGHVLASGG 
      GTADRHIRF WNCNIGSCI NSVDTKSQV CALLWSCNY 
      KEIISGHGF ANNELIIWK YPAMTKVAE LTGHTARVL 
      HLVMSPDGS TVLSAGADE TLRLWKCFV KNSTKTKTG 
      VQTLEKPSI LKQCIR
    CDS sequence
    • >TC001383 RA
      atgtcgcaa tttaaattc gtgacggaa atgaataat 
      ttggtgaca atggatggt ccattaact aagggtcct 
      ccaccccgc tggcaacga aaaggccaa gaaaattac 
      cagtcaagt aataatctc agtcttaac tcgtcaaag 
      caaaaatcg ttttcaaca tcaataacg aaaacgcca 
      actaaaagc gaggtaaaa tcgaaaaaa actccaaca 
      aaaacacca aacaagaac cgatcaaac ggttcgaaa 
      actccaaca ccgaacaaa accgcctcc aaaacgcct 
      gtagctgat cgttttata ccagtcagg agcatgagt 
      aattttgat ttggctcat tacaaactc agccaaaat 
      gaagatacg tccgatagt cccacgcag aaagaattg 
      caaaaagca atgtttgaa aacttgcat ggggccaat 
      atcgattcc cagaagatt ctttcgtac acaaataag 
      cctccgaca gctccagcc ggttttcaa aaccccatg 
      cgcgtcatt tatacacaa actaagact cccgcatcg 
      gttaaaagc aacaatcgt tatattcca caagctcca 
      gatcgaatt ttagatgct ccggatatt gttgatgat 
      tattatttg aatctgatg gactggagc gccggaaat 
      gttctagct gtggctctg ggggctcac gtttattta 
      tggaatgct ggaaccggt aacattgaa gatcttctg 
      gaattgcaa ggcaacgat tacgtttgt tctttggcc 
      tggattcaa gacggtgac catttggct gttggtaca 
      acaaatgga acagtagag ttatgggac tgtggacgc 
      gctaaacgt cttcgagta atggatggt cattcagca 
      cgtgttggt tcactctct tggaattcg tatgtattg 
      acaagtgga tgtcgtagc ggccaaata attcaccac 
      gatgtgcgt caacgtgaa catatcatt acgacactt 
      tctgggcat acacaagaa gtgtgtggt ttgaaatgg 
      tcaccagat ggccgctat ctagccagt ggaggcaac 
      gacaacgtc ttgaatatc tggcaaagt cagactgga 
      agccatcac accaataat cagccgctt catattttt 
      acagctcat caagctgct gttaaagca ttggcttgg 
      tgtccttgg caagggcat gttctggct agtggtggt 
      ggtacagca gatcgtcat attaggttt tggaattgc 
      aacattggc tcgtgtatt aattcagtt gacacgaaa 
      tcgcaagtt tgtgctctt ttgtggagt tgtaattat 
      aaggaaatc atttcggga catggtttt gctaataat 
      gaattgatt atatggaag tacccagct atgactaaa 
      gtagccgaa ttgaccgga catactgca agggtatta 
      catttagta atgtcgcct gatggcagt actgtgctg 
      tcagcaggt gctgatgag acactaaga ctatggaaa 
      tgctttgtt aagaactct acaaagacc aagactgga 
      gtgcagact cttgaaaaa ccgagcatt ctgaagcaa 
      tgtatcaga taa
    mRNA sequence
    • >TC001383 RA
      ttcaaattt caaaagaaa tgtaacggt tttcagttt 
      tctggcaga cgttgactg tgaaacagt atttgttcg 
      tttaaagct attttttgt gtttgaact tgtgctttc 
      cttgtgtgt cgttcggtt tagtgcaga atgtcgcaa 
      tttaaattc gtgacggaa atgaataat ttggtgaca 
      atggatggt ccattaact aagggtcct ccaccccgc 
      tggcaacga aaaggccaa gaaaattac cagtcaagt 
      aataatctc agtcttaac tcgtcaaag caaaaatcg 
      ttttcaaca tcaataacg aaaacgcca actaaaagc 
      gaggtaaaa tcgaaaaaa actccaaca aaaacacca 
      aacaagaac cgatcaaac ggttcgaaa actccaaca 
      ccgaacaaa accgcctcc aaaacgcct gtagctgat 
      cgttttata ccagtcagg agcatgagt aattttgat 
      ttggctcat tacaaactc agccaaaat gaagatacg 
      tccgatagt cccacgcag aaagaattg caaaaagca 
      atgtttgaa aacttgcat ggggccaat atcgattcc 
      cagaagatt ctttcgtac acaaataag cctccgaca 
      gctccagcc ggttttcaa aaccccatg cgcgtcatt 
      tatacacaa actaagact cccgcatcg gttaaaagc 
      aacaatcgt tatattcca caagctcca gatcgaatt 
      ttagatgct ccggatatt gttgatgat tattatttg 
      aatctgatg gactggagc gccggaaat gttctagct 
      gtggctctg ggggctcac gtttattta tggaatgct 
      ggaaccggt aacattgaa gatcttctg gaattgcaa 
      ggcaacgat tacgtttgt tctttggcc tggattcaa 
      gacggtgac catttggct gttggtaca acaaatgga 
      acagtagag ttatgggac tgtggacgc gctaaacgt 
      cttcgagta atggatggt cattcagca cgtgttggt 
      tcactctct tggaattcg tatgtattg acaagtgga 
      tgtcgtagc ggccaaata attcaccac gatgtgcgt 
      caacgtgaa catatcatt acgacactt tctgggcat 
      acacaagaa gtgtgtggt ttgaaatgg tcaccagat 
      ggccgctat ctagccagt ggaggcaac gacaacgtc 
      ttgaatatc tggcaaagt cagactgga agccatcac 
      accaataat cagccgctt catattttt acagctcat 
      caagctgct gttaaagca ttggcttgg tgtccttgg 
      caagggcat gttctggct agtggtggt ggtacagca 
      gatcgtcat attaggttt tggaattgc aacattggc 
      tcgtgtatt aattcagtt gacacgaaa tcgcaagtt 
      tgtgctctt ttgtggagt tgtaattat aaggaaatc 
      atttcggga catggtttt gctaataat gaattgatt 
      atatggaag tacccagct atgactaaa gtagccgaa 
      ttgaccgga catactgca agggtatta catttagta 
      atgtcgcct gatggcagt actgtgctg tcagcaggt 
      gctgatgag acactaaga ctatggaaa tgctttgtt 
      aagaactct acaaagacc aagactgga gtgcagact 
      cttgaaaaa ccgagcatt ctgaagcaa tgtatcaga 
      taattcatt attaataat aaattattc ttttacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY