Status
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Gene information
General information
TC001002 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9639598..9642583
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001002
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06132

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001002 RA
      MGHFEIIFI LLLTLQLWE TVSITRPVR NFHPPTDWE 
      LGLRESVHQ ALLREHYTD PGLRDRLLA APFINRQDD 
      ASPFGDIAV LARGQLYIT PEGFKFVQD VDCDCQRCL 
      TSPQCQWKT FGHHKLLAL WTVKRIRHR DPHYGHVYE 
      IEYTISPIT HTISEDLVK RHGFFNYNV PGHIFVIKS 
      DPGVNTVGQ KEWFKKEGD HGHYKPGIL IQMTPDGYK 
      HVLHSFPGF FTSYHDQTT IDPKEYELY KKLIESLAA 
      KTSSGPHRF SVTTPTTTT FVVSEGQLI PITTLPTQS 
      YTKSPKPIT TIHYYTPVE TTTEKQDAS FDIFSEPLR 
      TTTTKQITT SEERVNLFP VSPTEPMKQ VTEVPKHTR 
      PDSISEQLP APDRNVETT IPYVTTTQP SVRKEESTT 
      IEKVPYTEQ KTTIIGPFT TERVKSTTF YTTVPKSST 
      VIEAVPVRT LHTSTSPKT TSTSPKTTT IIEAVPVRT 
      LHTSTTFLP SSSPKTSSY ETTVIEAVP VRTLRPTTG 
      HDTTFSTSS SPKTTSYGT TVIEAVPVR TLHTSTNQG 
      TTFLPPSSP KTSYRTTLI EEVPVRTLH TSSSPKTTT 
      TIQTIPTTT PYGSTTIIE AVPVTTLQG STIIEAVPI 
      TTPYLSTSY GTTVVEAVP TTTHPKNTY ETIAKTPSY 
      ETTIPEIVP TTTRSRTSY GTTLGEPES VTTHPKPTT 
      VTKFYLEST PKTTVTQQK FTIPTTTTY YTQTPTQYL 
      KETTFTTKK VPRVTITTP SDDFLLDSD IEKKPKIPE 
      KKDFDVDDI FGTVSTKSS TEPMETTTK QLIQSDVSR 
      ALFGRAKQV KISSKIRKS TNRPKIIYG NFYAPSTPN 
      PTNTLLGTT VETDGYETT TEIRPSTDQ SISTSISFE 
      VNKRNKSED TTAPSTSET KKVFDDLAL QLVNHARTI 
      DYLQKKPSR QPKYRRRSF ATRKRSQRK NTKSAN
    CDS sequence
    • >TC001002 RA
      atgggacac ttcgaaata atcttcatt ttactcttg 
      actctccaa ctatgggaa actgtatca atcacccga 
      cccgtgcga aactttcat cctcccacc gactgggaa 
      ttggggctg cgtgagtcc gtgcatcag gccctcctt 
      cgggaacac tacaccgat ccggggctc cgcgaccgc 
      ctcctggca gcccccttt atcaaccga caggacgac 
      gcgagtcct ttcggagac atcgccgtt ctggcacga 
      ggacaactc tacataacc cctgaaggt ttcaaattt 
      gttcaagac gtggactgt gattgtcaa agatgcctc 
      acgtcgccc caatgccag tggaagacc ttcggtcac 
      cataaacta ctcgcactg tggacggtg aagcggata 
      agacatcgg gacccccat tatgggcac gtgtacgaa 
      attgagtac acgatttcg cccatcacg cacaccatc 
      agcgaggat ttggtcaaa agacatggg ttttttaac 
      tataacgtc ccgggacac attttcgtg attaagagc 
      gatccgggt gtgaacaca gttggacaa aaagagtgg 
      ttcaagaaa gagggtgat catggacac tacaaaccc 
      ggaatttta atacaaatg accccggat gggtacaaa 
      cacgtgttg cacagtttc cccggattt ttcacttca 
      taccacgac caaacaaca atcgatcca aaagaatac 
      gaactgtac aaaaaattg atcgagtca ttggcggcg 
      aaaacatca agtgggcct caccgattc tcagttacg 
      actcctacg actacgact tttgtcgta agtgaagga 
      caattaatc ccaattacg accttaccg acccaaagt 
      tatacaaag tcgccgaaa cccatcaca accattcat 
      tattacact ccggttgag acaacaacg gaaaaacaa 
      gacgcgagt tttgatatt ttttcggag ccgttacgc 
      acaactacc acgaaacaa attacgaca agtgaagaa 
      cgtgttaat ttgttcccg gttagtcca accgagccg 
      atgaaacag gtcacggaa gttccgaaa cacacacga 
      ccggattca atcagcgaa caacttcca gcccctgat 
      cgaaacgtt gaaactact attccgtac gttaccacg 
      actcaacct tcggttagg aaagaagaa agtaccaca 
      attgaaaaa gtaccgtat acagagcag aaaactaca 
      attattggg ccctttacg accgagagg gttaaaagt 
      actacgttt tataccact gtaccgaaa agtagtacg 
      gttattgag gcggtaccc gttaggact ttacacact 
      tcaaccagt cccaaaacc acttcgact agccccaaa 
      acaactact ataatagaa gcagttccc gtcagaact 
      ctgcacacg tcaaccact tttctacca tcgtctagc 
      cccaaaact agtagttat gaaaccaca gtaatagag 
      gcagtaccc gttcgaact ttacgacct acaactggt 
      catgatacc actttttcc acatcatcc agccccaaa 
      accaccagt tatggaacc actgtgata gaagcagtg 
      ccagtcaga actttgcac acttcaacc aatcaaggc 
      actactttt ctaccaccg tctagcccc aaaactagt 
      tatagaacg actctaata gaagaagta ccagttagg 
      acgctacac acttcaagt agtcctaaa actactact 
      acaatccaa acaataccc actacaact ccatatggc 
      tcaactact ataatagaa gcagtacca gtcacaacc 
      ctgcaaggt tctactatc atagaggcg gtacctatc 
      acaactcca tacctgtca actagttat ggtaccact 
      gtagtagag gcggtacct actacaact cacccgaaa 
      aatacatac gagacaata gcaaaaaca cccagttat 
      gaaaccact ataccagag atagtacct acgaccact 
      cggagtcga acgagttat ggtaccact ttaggagaa 
      cccgaatct gtgacaacc caccctaaa cccaccaca 
      gtcacgaaa ttttattta gaatcaacg ccaaaaaca 
      acagtaacg caacaaaaa ttcacaatc cctacgact 
      accacttat tatacgcaa accccgact caataccta 
      aaagaaacg actttcaca acaaagaaa gtacctcga 
      gtgaccata actacccca agcgacgat ttcttgctc 
      gattcagac atcgagaaa aaaccgaaa atacccgag 
      aaaaaagat ttcgacgtc gatgatatt ttcggaacg 
      gtgtcgaca aaatcgagt accgaacca atggaaaca 
      actacgaaa caattgatt caatctgat gtttcccgg 
      gctttattc gggcgcgca aaacaagtg aagatcagt 
      tcgaaaata agaaaatca acgaaccga ccgaaaatt 
      atttatgga aatttctac gccccctcg actcccaat 
      cccactaat acccttttg ggtactact gtggaaacg 
      gatggatac gaaacgacg accgaaatt cggccatcg 
      accgatcag agcatcagc acgtcgata agtttcgaa 
      gtaaataag agaaataaa tcggaggat acaacagcg 
      ccaagtaca agcgaaacg aaaaaagtt ttcgacgat 
      ttggccctc caattagtg aatcatgcc cgaactatc 
      gattactta cagaaaaaa ccgtcaaga cagccgaaa 
      tatcgcaga cgaagcttt gcgacgcga aaacgaagt 
      cagagaaaa aatactaag agtgcgaat tga
    mRNA sequence
    • >TC001002 RA
      agtaaaaag atttagttg taagaaacc gagcgaact 
      agtgacatg tgcagcttt caaacattt gcaaacacc 
      cagtgggtc taaaatggg acacttcga aataatctt 
      cattttact cttgactct ccaactatg ggaaactgt 
      atcaatcac ccgacccgt gcgaaactt tcatcctcc 
      caccgactg ggaattggg gctgcgtga gtccgtgca 
      tcaggccct ccttcggga acactacac cgatccggg 
      gctccgcga ccgcctcct ggcagcccc ctttatcaa 
      ccgacagga cgacgcgag tcctttcgg agacatcgc 
      cgttctggc acgaggaca actctacat aacccctga 
      aggtttcaa atttgttca agacgtgga ctgtgattg 
      tcaaagatg cctcacgtc gccccaatg ccagtggaa 
      gaccttcgg tcaccataa actactcgc actgtggac 
      ggtgaagcg gataagaca tcgggaccc ccattatgg 
      gcacgtgta cgaaattga gtacacgat ttcgcccat 
      cacgcacac catcagcga ggatttggt caaaagaca 
      tgggttttt taactataa cgtcccggg acacatttt 
      cgtgattaa gagcgatcc gggtgtgaa cacagttgg 
      acaaaaaga gtggttcaa gaaagaggg tgatcatgg 
      acactacaa acccggaat tttaataca aatgacccc 
      ggatgggta caaacacgt gttgcacag tttccccgg 
      atttttcac ttcatacca cgaccaaac aacaatcga 
      tccaaaaga atacgaact gtacaaaaa attgatcga 
      gtcattggc ggcgaaaac atcaagtgg gcctcaccg 
      attctcagt tacgactcc tacgactac gacttttgt 
      cgtaagtga aggacaatt aatcccaat tacgacctt 
      accgaccca aagttatac aaagtcgcc gaaacccat 
      cacaaccat tcattatta cactccggt tgagacaac 
      aacggaaaa acaagacgc gagttttga tattttttc 
      ggagccgtt acgcacaac taccacgaa acaaattac 
      gacaagtga agaacgtgt taatttgtt cccggttag 
      tccaaccga gccgatgaa acaggtcac ggaagttcc 
      gaaacacac acgaccgga ttcaatcag cgaacaact 
      tccagcccc tgatcgaaa cgttgaaac tactattcc 
      gtacgttac cacgactca accttcggt taggaaaga 
      agaaagtac cacaattga aaaagtacc gtatacaga 
      gcagaaaac tacaattat tgggccctt tacgaccga 
      gagggttaa aagtactac gttttatac cactgtacc 
      gaaaagtag tacggttat tgaggcggt acccgttag 
      gactttaca cacttcaac cagtcccaa aaccacttc 
      gactagccc caaaacaac tactataat agaagcagt 
      tcccgtcag aactctgca cacgtcaac cacttttct 
      accatcgtc tagccccaa aactagtag ttatgaaac 
      cacagtaat agaggcagt acccgttcg aactttacg 
      acctacaac tggtcatga taccacttt ttccacatc 
      atccagccc caaaaccac cagttatgg aaccactgt 
      gatagaagc agtgccagt cagaacttt gcacacttc 
      aaccaatca aggcactac ttttctacc accgtctag 
      ccccaaaac tagttatag aacgactct aatagaaga 
      agtaccagt taggacgct acacacttc aagtagtcc 
      taaaactac tactacaat ccaaacaat acccactac 
      aactccata tggctcaac tactataat agaagcagt 
      accagtcac aaccctgca aggttctac tatcataga 
      ggcggtacc tatcacaac tccatacct gtcaactag 
      ttatggtac cactgtagt agaggcggt acctactac 
      aactcaccc gaaaaatac atacgagac aatagcaaa 
      aacacccag ttatgaaac cactatacc agagatagt 
      acctacgac cactcggag tcgaacgag ttatggtac 
      cactttagg agaacccga atctgtgac aacccaccc 
      taaacccac cacagtcac gaaatttta tttagaatc 
      aacgccaaa aacaacagt aacgcaaca aaaattcac 
      aatccctac gactaccac ttattatac gcaaacccc 
      gactcaata cctaaaaga aacgacttt cacaacaaa 
      gaaagtacc tcgagtgac cataactac cccaagcga 
      cgatttctt gctcgattc agacatcga gaaaaaacc 
      gaaaatacc cgagaaaaa agatttcga cgtcgatga 
      tattttcgg aacggtgtc gacaaaatc gagtaccga 
      accaatgga aacaactac gaaacaatt gattcaatc 
      tgatgtttc ccgggcttt attcgggcg cgcaaaaca 
      agtgaagat cagttcgaa aataagaaa atcaacgaa 
      ccgaccgaa aattattta tggaaattt ctacgcccc 
      ctcgactcc caatcccac taataccct tttgggtac 
      tactgtgga aacggatgg atacgaaac gacgaccga 
      aattcggcc atcgaccga tcagagcat cagcacgtc 
      gataagttt cgaagtaaa taagagaaa taaatcgga 
      ggatacaac agcgccaag tacaagcga aacgaaaaa 
      agttttcga cgatttggc cctccaatt agtgaatca 
      tgcccgaac tatcgatta cttacagaa aaaaccgtc 
      aagacagcc gaaatatcg cagacgaag ctttgcgac 
      gcgaaaacg aagtcagag aaaaaatac taagagtgc 
      gaattgact gaagtgaat taaaaattg cacaatgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY