Status
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Gene information
General information
TC000746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6338034..6341740
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000746
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06129

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000746 RA
      MAKREKRAN AGNRMAKLL DEEEECQDE FYKTNYGGF 
      EETESDREY EEEEEGEDV VDSDFSIDE NDEPISDNE 
      EEGQKKKRR LVTKAYKEP VPAPSKQKA KPKPTKPVP 
      KVRTTQHES TEYERKSIR KSTAAKSAA TAQRIKVRN 
      LEQKKKIKK PKEEEWVPT QEELLEEAK TTELENLKS 
      LEKYQKMES EKKTKRTVK KVINGPVIQ YRSTRMPII 
      EEINQDKDI KNESENVCE RTFISVLND PNDATFNSI 
      FEKKTARPL PRRLRCIVT GHLAKYVDP KTCLPYHSS 
      MCLKIIRSA YYQQLEAHG DKSNPVVAN WLKWYVKNK 
      DKLRRELAL KVKTHQVDH S
    CDS sequence
    • >TC000746 RA
      atggcaaaa agagaaaaa cgggcaaat gccggtaac 
      cgtatggca aaattgtta gatgaagaa gaagaatgt 
      caagacgaa ttctacaaa accaattat ggtggtttt 
      gaagaaacc gaatccgat agggaatac gaggaggag 
      gaagaaggc gaagatgtg gtagattcc gactttagt 
      attgacgaa aatgacgaa cctatttca gacaatgag 
      gaggagggt cagaagaag aaacgaaga ttagttaca 
      aaggcttac aaggaacca gtgcctgca cccagtaaa 
      caaaaagca aaaccaaaa cccacaaaa cctgtccct 
      aaagtcaga acaacccaa catgaatcc acagaatac 
      gaaagaaag tcaatcagg aagtccaca gctgcaaaa 
      tcagccgca acagcacaa cgcatcaaa gtacgaaat 
      ctcgaacaa aagaaaaaa atcaagaaa ccgaaagaa 
      gaagaatgg gtgccaaca caggaagag ttactcgaa 
      gaagcaaaa acgaccgaa ttggagaac ttgaagtca 
      cttgaaaag taccaaaag atggaaagt gagaagaaa 
      acgaaacga actgtgaaa aaagtcatc aatggccct 
      gttatccaa tacagatca acacgaatg ccaatcatt 
      gaggaaatc aatcaagat aaagacatc aagaacgaa 
      tcagaaaac gtgtgcgaa cgcacgttt atctcagtg 
      cttaatgac cctaacgat gccacattt aacagcatt 
      tttgaaaaa aagacagcg cgtccgctt ccacgacgt 
      ctcagatgc attgtcaca ggtcacctt gcaaagtac 
      gttgaccct aaaacgtgt ctgccttac cacagcagc 
      atgtgtttg aagataatt cgttctgct tattaccaa 
      caactggaa gctcatgga gataagagt aatccagta 
      gttgcaaac tggcttaag tggtacgtt aagaataag 
      gataagtta aggagggag ttggcgttg aaagtaaaa 
      acacatcag gtggatcac agttga
    mRNA sequence
    • >TC000746 RA
      taaaatttc gcagtttga cagatggtg tttagtgct 
      tgtgttttt tatttaatt aggaaaaat attaaataa 
      aaaccattt actttgtaa taaaactta tcattggtt 
      tgttgtccc tgtcccagg cgagaaatt cgttacaat 
      ggcaaaaag agaaaaacg ggcaaatgc cggtaaccg 
      tatggcaaa attgttaga tgaagaaga agaatgtca 
      agacgaatt ctacaaaac caattatgg tggttttga 
      agaaaccga atccgatag ggaatacga ggaggagga 
      agaaggcga agatgtggt agattccga ctttagtat 
      tgacgaaaa tgacgaacc tatttcaga caatgagga 
      ggagggtca gaagaagaa acgaagatt agttacaaa 
      ggcttacaa ggaaccagt gcctgcacc cagtaaaca 
      aaaagcaaa accaaaacc cacaaaacc tgtccctaa 
      agtcagaac aacccaaca tgaatccac agaatacga 
      aagaaagtc aatcaggaa gtccacagc tgcaaaatc 
      agccgcaac agcacaacg catcaaagt acgaaatct 
      cgaacaaaa gaaaaaaat caagaaacc gaaagaaga 
      agaatgggt gccaacaca ggaagagtt actcgaaga 
      agcaaaaac gaccgaatt ggagaactt gaagtcact 
      tgaaaagta ccaaaagat ggaaagtga gaagaaaac 
      gaaacgaac tgtgaaaaa agtcatcaa tggccctgt 
      tatccaata cagatcaac acgaatgcc aatcattga 
      ggaaatcaa tcaagataa agacatcaa gaacgaatc 
      agaaaacgt gtgcgaacg cacgtttat ctcagtgct 
      taatgaccc taacgatgc cacatttaa cagcatttt 
      tgaaaaaaa gacagcgcg tccgcttcc acgacgtct 
      cagatgcat tgtcacagg tcaccttgc aaagtacgt 
      tgaccctaa aacgtgtct gccttacca cagcagcat 
      gtgtttgaa gataattcg ttctgctta ttaccaaca 
      actggaagc tcatggaga taagagtaa tccagtagt 
      tgcaaactg gcttaagtg gtacgttaa gaataagga 
      taagttaag gagggagtt ggcgttgaa agtaaaaac 
      acatcaggt ggatcacag ttgatttat tttgctcta 
      tttaataaa tttttataa tacagtttc tatttcttt 
      caaataaaa ctgttttgg tctgagatc aacacgatt 
      aaatgttag tgaatgagt ttgcaacaa atttggacg 
      aatgaaaag cgtcgaaaa cgtttagtt aatatattt 
      attaaactg gtgcacaaa taatacatt accataatc 
      tcagtagcc aataatttg gctgaaaat tggcttatg 
      aagtacaac aagaattaa taacattaa atattatta 
      atatgacac ataaattac atctagttt aacatatca 
      tataaaatt aataggtat ttgataagc tacctcatt 
      aattaatgc cattattac tttgttaaa ataatttta 
      gcacttgag tatgaacag tgtatatca aaagagtag 
      ttcgattaa caatgcgaa tttgttaat agattactg 
      ttttaatat acaattcgt taaaactct gagaaaacg 
      aaattaatc tctaatcta agtacaaca taatgtaaa 
      tatttataa tagttaaaa aatctttaa agtactctc 
      agcaaaaca atttcatgt ccgaaaaca attttcaat 
      aaaacattc tctacaaat tgcacatgt ctgatagta 
      acactattg cacgaaaaa aggtaaaga ttctcgtta 
      caacaaaat gagtatgat tctagattt gatatttat 
      tgcttactt tggacggaa aaaaatgag ataatccgc 
      acgtattgc ttaaataaa tttgagaaa ctaaaaaat 
      taacaataa ataatataa aacaccgcg ctgatactt 
      tttaaaaat taaaagtaa atagagccc tccttcaac 
      gtttgaaac taatttcca aaagtataa aaatgaatc 
      gattattat tgtcctaca taatatcaa tttgttcga 
      cctttgcat aaataacat ctacatctt taagtaact 
      taaacatac ctaccttac attgcaatt ttttcacaa 
      actgatcaa atcgaaaac aggaaaatt cagtaagta 
      cagttcaac taagtttag attcaatta aaagtgcca 
      cacctaaca gaattttcc taattctct atctatttt 
      acatgtgca cgattaaca ttattggca cgcaaaaag 
      tttgtactt tacagaaaa attgtaaca agtattagt 
      aaaactgga tattgacaa tatccacaa tctctcagg 
      tatgtgtaa aaaagagca agcaaaact ctacggaca 
      gatctggtt tgagattct ttcgaaaac aactgattg 
      agattccgt gtaattttg ctcgggacg taacaaaca 
      cacttacaa tgttattgc ttccaaaga ttacgtttc 
      ctttcttta gtacacaat gcacgatga cggagtgaa 
      aaaaatgtt agtgatgta atgttagac gtaccgcta 
      cctcagatg gtccggtgt ttccgttag tggagtggc 
      gcaggtgac aggttagca ctccaagcc tacaggccg 
      ggcaaccag gttaacaag aaaagaaaa gaaaacagt 
      ttgttagta ttgtatcat agctatacc acgggcaca 
      attcaatac aatttatcc cagtgtcac tcatgcttg 
      gttcagtgt gattagagg cttcacctc atgcgttaa 
      ggcctgtga cactggcat aacattgag caatttata 
      catacacat aaaatactg atttaacct agcagcaag 
      tgttcgata gactatatt aaagtgctc gaagagatt 
      cggtgctag aagaatttc tctgtgtat gtaccgcaa 
      ttaattaga aggcattgt tgttattaa actgtacaa 
      aaggttaat ttgcaaata acattttaa tatagtgct 
      atgaacaca gtcacacgt tatcatcaa ccatacatt 
      agtttactt ggccatgtg gaattagaa gtagttatg 
      aaatcttgt ggagaagtg ttaatgcat tattagaag 
      ttaataaat aataaaact ttgataatt cc
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