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Gene information
General information
TC000237 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14319919..14328311
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000237
    iBeetle screen iB_06125

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000237 RA
      MDMDNKNMI NDDSNVTLT IRLIMQGKE VGSIIGKKG 
EIVKRFREE SGAKINISD GSCPERIVT VTGSTNAIF 
KAFTLICKK FEEWCSQFQ DINSGGSGV PRPPITLRL 
IVPASQCGS LIGKGGSKI KEIREVTGA SIQVASEML 
PNSTERAVT ISGTGEAIT QCIYHICTV MLESPPKGA 
TIPYRPKPQ VGGPVILAG GQAYTIQGN YAVPAHTDM 
SALGKSPLA GLAALGLGG LAPTNTGGL NPAALAALA 
GSQLRTSNS RNQQNSNQQ THEMTVPNE LIGCIIGKG 
GTKIAEIRQ ISGAMIRIS NCDDRESGV TDRTITISG 
NPDAVALAQ YLINMSVEL QKANLEAQN SPSTGGSSQ 
SSTAASPLA SAIPLAQLL AKPGALNAL TSLSALGGL 
TELLGGQAG APPVQTTGV HRTHKSYTP RLRSPGGSG 
GPSEGSKLK SERNKFNPY
    CDS sequence
    • >TC000237 RA
      atggatatg gataataaa aatatgata aacgatgac 
tcgaatgtc acgctcacg attaggctt attatgcaa 
ggaaaggaa gtgggcagt atcataggc aaaaaagga 
gaaattgtg aaaagattt agggaagag tctggtgcc 
aaaataaac atatctgac gggtcgtgc cctgaacgt 
attgtaaca gtaacggga tcaaccaat gcaattttt 
aaagctttc acactaatt tgtaaaaaa ttcgaggag 
tggtgttcc cagttccag gacataaat agtggaggt 
agcggtgta ccaagacct ccgattacg ttgcgactc 
atcgtacct gcaagccaa tgtggatct ctcatcggt 
aaaggaggg tccaaaatc aaagaaatt agggaggtc 
acgggcgcc tccatccag gtcgcctcc gaaatgctg 
cccaactcg acggagagg gccgtcacc atttccgga 
accggagaa gcaatcacg cagtgcatt tatcacatc 
tgtaccgtt atgctcgag tcccctccc aaaggagca 
accatcccc taccgcccc aaaccccaa gtgggtggc 
cccgtgatc ctggccggc ggacaagcc tataccatc 
caaggcaac tacgccgtt cctgcccat acagatatg 
agtgcactt ggtaaaagt cctctcgca ggactagca 
gccttagga ctaggaggc cttgcaccg accaacact 
ggtggatta aatccagca gcgttggca gctctcgcc 
ggatctcag cttcgaacg tcaaactcc cgaaaccag 
caaaattcc aatcagcaa acccacgag atgaccgtt 
ccgaacgag ctaatcggt tgcattatt ggcaagggc 
ggtacaaag atcgccgaa atccggcag atttccgga 
gcgatgatc cgcatatcg aactgtgat gaccgtgaa 
agtggcgtt accgaccgt acaattacc atctctggc 
aatccggat gcggtggct ttagcgcag tatctcatc 
aatatgagt gttgagctg cagaaagct aaccttgag 
gcccaaaat tctcctagc actggcggg tccagccag 
tcctccacc gctgcctct cccctggcc agtgccatt 
cctctagcc caactcttg gccaagccg ggtgccctc 
aacgcattg acgagcctc agtgcgctg ggcggttta 
accgaattg ttgggcggg caggcaggc gctcctcct 
gtccagacc accggcgtt caccgcacc cacaagagc 
tacactcct cggctccgt tctccgggc ggcagtggc 
gggccttcc gagggctcc aagctcaag agcgagagg 
aacaagttc aatccttat taa
    mRNA sequence
    • >TC000237 RA
      catcagttg gcagcattg tcgaatgaa gcacgtgga 
gagcaagcg acggcgttt ccaagctcc cgaataatt 
taatttttt acctgtttg tgggttcaa aaattgaac 
ccaaaaacc taccccttc gccctgacc cccgtttcg 
caagaccat ggatatgga taataaaaa tatgataaa 
cgatgactc gaatgtcac gctcacgat taggcttat 
tatgcaagg aaaggaagt gggcagtat cataggcaa 
aaaaggaga aattgtgaa aagatttag ggaagagtc 
tggtgccaa aataaacat atctgacgg gtcgtgccc 
tgaacgtat tgtaacagt aacgggatc aaccaatgc 
aatttttaa agctttcac actaatttg taaaaaatt 
cgaggagtg gtgttccca gttccagga cataaatag 
tggaggtag cggtgtacc aagacctcc gattacgtt 
gcgactcat cgtacctgc aagccaatg tggatctct 
catcggtaa aggagggtc caaaatcaa agaaattag 
ggaggtcac gggcgcctc catccaggt cgcctccga 
aatgctgcc caactcgac ggagagggc cgtcaccat 
ttccggaac cggagaagc aatcacgca gtgcattta 
tcacatctg taccgttat gctcgagtc ccctcccaa 
aggagcaac catccccta ccgccccaa accccaagt 
gggtggccc cgtgatcct ggccggcgg acaagccta 
taccatcca aggcaacta cgccgttcc tgcccatac 
agatatgag tgcacttgg taaaagtcc tctcgcagg 
actagcagc cttaggact aggaggcct tgcaccgac 
caacactgg tggattaaa tccagcagc gttggcagc 
tctcgccgg atctcagct tcgaacgtc aaactcccg 
aaaccagca aaattccaa tcagcaaac ccacgagat 
gaccgttcc gaacgagct aatcggttg cattattgg 
caagggcgg tacaaagat cgccgaaat ccggcagat 
ttccggagc gatgatccg catatcgaa ctgtgatga 
ccgtgaaag tggcgttac cgaccgtac aattaccat 
ctctggcaa tccggatgc ggtggcttt agcgcagta 
tctcatcaa tatgagtgt tgagctgca gaaagctaa 
ccttgaggc ccaaaattc tcctagcac tggcgggtc 
cagccagtc ctccaccgc tgcctctcc cctggccag 
tgccattcc tctagccca actcttggc caagccggg 
tgccctcaa cgcattgac gagcctcag tgcgctggg 
cggtttaac cgaattgtt gggcgggca ggcaggcgc 
tcctcctgt ccagaccac cggcgttca ccgcaccca 
caagagcta cactcctcg gctccgttc tccgggcgg 
cagtggcgg gccttccga gggctccaa gctcaagag 
cgagaggaa caagttcaa tccttatta atggacctg 
cgcctctaa ccttctcaa agacactac tttttacaa 
atttactgc tttctttat ttttcgtta ctttatacg 
gcccgggaa gggagcacg aagtactcg gctcgatat 
tgtgaggag ataactgat aattcactt taatttttt 
atcgacggt ttaagtttt ctggcagag cttggcgcg 
tgctggatt tatttatat catgtaact aattacata 
gacttcgtt attatgtgt ttctttgcg attagtact 
tgctgagat acaatagag atagttttg tacaaaatt 
cgatgcctg ttttgtagg cagctttgt tgttgacat 
atcagcagc attaagatt ttaaagata tctgtgtgt 
tttctatag attttcgac ccttctcaa agagctttt 
caatattta agtttaaaa caacgtaaa tcatacaat 
aatgagtaa gcattcata tacataagt gtttgttta 
gaatctaag tttgttttt atgtaggtc gttctcttt 
agagacctt aattttata ttagtgtct acaacttaa 
gatttcagt gtatttaca tagagacca atacattat 
gtgtgttta ataaaattt tctttatta a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY