Status
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Gene information
General information
TC010477 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451027.1: 145434..155218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010477
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06112

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010477 RA
      MDTEPKGEV QPPATKPTS ASPPKKKHK KDHKHKHKK 
      HVTEKLDKT KKTKKRKKH KHNKQRDMS EDRLSTKSD 
      KLDRYTPEL SPKSATAVC KKNVNHIIN GKSLSGTPK 
      NKDPLKTDT DEVVAFVAR GLTDTHSLE VISSESEDG 
      VPETECDSD AIDVNVIEA DMDLEELMK QKELLQAEI 
      AKAELETTK KNGKDPKIV DEIILLDDS SNDGDVVVP 
      VKKPKRSHS RERRVVIER KEHPREDVR RHRDDKHKV 
      YERYGRDNY HRTNHRKSP ERRERSRSH IYDRDKRHV 
      RDHRSRERH RSRDRRYDH KDYYHRDYH RNDTRKAKE 
      RHRGNRGEK QDKYKDSLS EGQKHGVSS DSDIDVNID 
      LVDDEEDEQ KIIEKRRKQ REELLKRLG GVSEDSNTN 
      TSLPTSPKE EKESTNDTA PPTEPEKPA QEKTPEKKE 
      EPEPEESHT PPLLPNMKV KPVSNEKEK EPKTKRPNW 
      DMFADQDIF KEDTNSPNT IVTKGAGAE NPALTDNWD 
      DAEGYYRVR IGEILDSRY MVYGYTGQG VFSNVVRAR 
      DQARSNLDV AVKIIRNNE IMHKTGLKE LEILKKLND 
      ADPEDKLHC LRLIRHFFH KQHLCMVFE PLAMNLREV 
      LKKYGKDVG LHIKAVRSY TQQLLLALK LLKKTGILH 
      ADIKPDNIL VNESKLVLK LCDFGSASY INDNEITPY 
      LVSRFYRSP EIILGIPYD YGIDMWSAA CTIYELYTG 
      RILFSGKSN NQMLKFFMD VKGKFPNKV IRKGAFRDQ 
      HFDSNCNFL YHEIDRVTE REKVVVMSV VKVTRDLQS 
      ELVAGQALP PDQLRKVTQ LKDMLEKAL TIDPAKRIS 
      LNNALTHPF IQDKI
    CDS sequence
    • >TC010477 RA
      atggacacc gaacccaaa ggtgaagtc cagccgccc 
      gccaccaag cccacctcc gccagcccc cctaagaaa 
      aaacacaag aaagaccac aaacacaaa cacaaaaag 
      cacgtcacc gaaaaacta gataaaact aaaaagacc 
      aagaaacgc aaaaagcac aaacacaac aaacaaagg 
      gacatgtcg gaagacagg ctttcgaca aaaagtgac 
      aaactagat cggtatacc cccgaatta tcgcctaag 
      tcagcaact gcagtatgt aagaaaaat gttaatcat 
      attattaat ggtaaatct ttgtcaggg accccaaaa 
      aacaaggac ccgcttaaa actgacact gatgaggtt 
      gttgctttt gtggcgcgc ggtctcacc gacacgcac 
      tcgctggag gtgatctcc tccgagagc gaggacggc 
      gtgccggag acagagtgc gatagcgac gcgatcgac 
      gtcaacgtc atcgaggcc gacatggac ctggaggag 
      ctcatgaag cagaaggag ctgctccag gccgagatc 
      gccaaagcg gagctcgaa acgaccaaa aagaacgga 
      aaagacccg aaaattgtt gacgaaatc atcctcttg 
      gacgattcg agcaacgac ggggacgtg gtggtgcct 
      gtgaagaag cccaaaagg tcccacagc agagagcgc 
      agagtcgtc attgagcgc aaagagcat ccaagagag 
      gatgtgcga aggcataga gacgacaaa cacaaagtg 
      tacgagaga tacgggcga gataattat cacaggacc 
      aaccacagg aaaagtccc gagcggagg gagaggtcc 
      aggtcgcat atttatgac agggataag cgacatgtg 
      agagatcac aggtcgagg gagagacat agatcgcgg 
      gatcggcga tatgatcat aaggattat tatcatcgg 
      gattatcac aggaatgat acgagaaag gcgaaggag 
      aggcataga gggaatagg ggtgagaag caggataag 
      tacaaggac tccctgagt gagggacag aagcatggg 
      gttagcagt gatagcgat attgatgtt aatatcgat 
      ctggtggat gatgaagaa gatgagcag aagattatt 
      gagaagagg aggaagcaa agggaagag ttgttgaag 
      cggttgggt ggtgtcagc gaggattca aacaccaac 
      acatcgctg ccgacatca ccaaaggaa gaaaaggaa 
      agtacaaac gatactgca ccaccaacc gagcccgaa 
      aaaccagct caagagaag acaccagag aagaaagaa 
      gaaccagaa ccggaagaa tcacacact ccacctcta 
      ctacccaac atgaaggta aaaccagtc tcaaacgag 
      aaagaaaaa gaacccaaa accaagaga cccaattgg 
      gacatgttt gccgaccag gacattttc aaagaagat 
      acaaattcc cccaatacc atcgttacg aaaggtgca 
      ggcgccgaa aatccggcc ttaaccgac aattgggac 
      gatgccgaa ggttactac agggtcaga attggggaa 
      attttggat tctagatac atggtgtat ggctacact 
      ggacaaggt gttttcagt aacgtggta agagctcgc 
      gaccaagcc agatcgaat cttgatgtt gcagtcaaa 
      atcattcga aataacgaa ataatgcat aaaaccggg 
      ttaaaagaa ttggaaatt ttgaaaaaa ttgaacgat 
      gccgatccg gaagacaag ttgcattgt ttgcgcttg 
      attcggcac tttttccac aaacagcat ttatgtatg 
      gtttttgaa ccgttggcg atgaatctg agagaagtt 
      ttgaaaaag tatggaaaa gatgttggt ttgcatatt 
      aaagccgtg agaagctac acacagcag ttgttgttg 
      gccttgaag ctgctcaag aagactggc attttacat 
      gcagatatc aaaccagac aatatatta gtcaatgag 
      agcaaactt gtgctcaaa ctgtgcgat ttcgggtca 
      gcttcttac ataaacgat aacgaaatc acaccgtat 
      ttggtgtct agattttat agatcgcca gaaatcatt 
      ctggggatt ccatacgat tatggaatt gatatgtgg 
      tctgctgcg tgtaccatt tatgagcta tacactggt 
      aggatatta ttttctggt aaatccaac aatcagatg 
      ttgaagttc tttatggac gttaagggg aaatttccg 
      aataaagtg attcgaaaa ggggcattt agggatcaa 
      catttcgac agcaactgc aattttctc taccatgag 
      atagatcga gttactgaa cgggaaaaa gtcgtggtg 
      atgtccgtt gttaaagtg actcgagac ctccaatcc 
      gagctggtg gcaggtcag gctttgccc ccggaccag 
      cttcgaaaa gtgacccaa ctgaaggat atgttggag 
      aaggcattg actatagat cctgccaaa aggatcagc 
      ttgaataat gcgttaaca catccgttt attcaggac 
      aagatttga
    mRNA sequence
    • >TC010477 RA
      accgttggc ctggcgatt cgatattcc gatgttatt 
      cctaggtta tttttgaaa taaaattac cactaatca 
      gccactaat ttgccattt cgaccacat ttccccgcc 
      accggaatg gacaccgaa cccaaaggt gaagtccag 
      ccgcccgcc accaagccc acctccgcc agcccccct 
      aagaaaaaa cacaagaaa gaccacaaa cacaaacac 
      aaaaagcac gtcaccgaa aaactagat aaaactaaa 
      aagaccaag aaacgcaaa aagcacaaa cacaacaaa 
      caaagggac atgtcggaa gacaggctt tcgacaaaa 
      agtgacaaa ctagatcgg tataccccc gaattatcg 
      cctaagtca gcaactgca gtatgtaag aaaaatgtt 
      aatcatatt attaatggt aaatctttg tcagggacc 
      ccaaaaaac aaggacccg cttaaaact gacactgat 
      gaggttgtt gcttttgtg gcgcgcggt ctcaccgac 
      acgcactcg ctggaggtg atctcctcc gagagcgag 
      gacggcgtg ccggagaca gagtgcgat agcgacgcg 
      atcgacgtc aacgtcatc gaggccgac atggacctg 
      gaggagctc atgaagcag aaggagctg ctccaggcc 
      gagatcgcc aaagcggag ctcgaaacg accaaaaag 
      aacggaaaa gacccgaaa attgttgac gaaatcatc 
      ctcttggac gattcgagc aacgacggg gacgtggtg 
      gtgcctgtg aagaagccc aaaaggtcc cacagcaga 
      gagcgcaga gtcgtcatt gagcgcaaa gagcatcca 
      agagaggat gtgcgaagg catagagac gacaaacac 
      aaagtgtac gagagatac gggcgagat aattatcac 
      aggaccaac cacaggaaa agtcccgag cggagggag 
      aggtccagg tcgcatatt tatgacagg gataagcga 
      catgtgaga gatcacagg tcgagggag agacataga 
      tcgcgggat cggcgatat gatcataag gattattat 
      catcgggat tatcacagg aatgatacg agaaaggcg 
      aaggagagg catagaggg aataggggt gagaagcag 
      gataagtac aaggactcc ctgagtgag ggacagaag 
      catggggtt agcagtgat agcgatatt gatgttaat 
      atcgatctg gtggatgat gaagaagat gagcagaag 
      attattgag aagaggagg aagcaaagg gaagagttg 
      ttgaagcgg ttgggtggt gtcagcgag gattcaaac 
      accaacaca tcgctgccg acatcacca aaggaagaa 
      aaggaaagt acaaacgat actgcacca ccaaccgag 
      cccgaaaaa ccagctcaa gagaagaca ccagagaag 
      aaagaagaa ccagaaccg gaagaatca cacactcca 
      cctctacta cccaacatg aaggtaaaa ccagtctca 
      aacgagaaa gaaaaagaa cccaaaacc aagagaccc 
      aattgggac atgtttgcc gaccaggac attttcaaa 
      gaagataca aattccccc aataccatc gttacgaaa 
      ggtgcaggc gccgaaaat ccggcctta accgacaat 
      tgggacgat gccgaaggt tactacagg gtcagaatt 
      ggggaaatt ttggattct agatacatg gtgtatggc 
      tacactgga caaggtgtt ttcagtaac gtggtaaga 
      gctcgcgac caagccaga tcgaatctt gatgttgca 
      gtcaaaatc attcgaaat aacgaaata atgcataaa 
      accgggtta aaagaattg gaaattttg aaaaaattg 
      aacgatgcc gatccggaa gacaagttg cattgtttg 
      cgcttgatt cggcacttt ttccacaaa cagcattta 
      tgtatggtt tttgaaccg ttggcgatg aatctgaga 
      gaagttttg aaaaagtat ggaaaagat gttggtttg 
      catattaaa gccgtgaga agctacaca cagcagttg 
      ttgttggcc ttgaagctg ctcaagaag actggcatt 
      ttacatgca gatatcaaa ccagacaat atattagtc 
      aatgagagc aaacttgtg ctcaaactg tgcgatttc 
      gggtcagct tcttacata aacgataac gaaatcaca 
      ccgtatttg gtgtctaga ttttataga tcgccagaa 
      atcattctg gggattcca tacgattat ggaattgat 
      atgtggtct gctgcgtgt accatttat gagctatac 
      actggtagg atattattt tctggtaaa tccaacaat 
      cagatgttg aagttcttt atggacgtt aaggggaaa 
      tttccgaat aaagtgatt cgaaaaggg gcatttagg 
      gatcaacat ttcgacagc aactgcaat tttctctac 
      catgagata gatcgagtt actgaacgg gaaaaagtc 
      gtggtgatg tccgttgtt aaagtgact cgagacctc 
      caatccgag ctggtggca ggtcaggct ttgcccccg 
      gaccagctt cgaaaagtg acccaactg aaggatatg 
      ttggagaag gcattgact atagatcct gccaaaagg 
      atcagcttg aataatgcg ttaacacat ccgtttatt 
      caggacaag atttgaggg ttggtctag tgaggtaac 
      cggacaccg cgagtaatt ttaattttt ttgtttgtt 
      attattttt ctttgaatc ctttaaaag tcttgtact 
      agacttata ttttattaa aacaaactg tagattaac 
      aatgttgta cacgaaaat tcatattgt ggagtattt 
      ataaagtaa taaaatatt gataaaagt ttgtcattt 
      ttgttacag aagtgtggc gaaatgagt gtttggata 
      ggaggtctt ggtgtaaag ttcgataag gtatttcgc 
      ctgcgactg ttataaaaa ttgtgctca caatttttt 
      caaaaaata cattttgat ttgtaattt tttcatcaa 
      aatcagaat gtattttac tgacttgac ctttgtttt 
      cgcaatgac tttgcttaa acagaggtg aacaaattg 
      ctgattatt tctttaaaa taaaactgc gaaacccaa 
      actttgtgg taaattttc ttttcattt tcagctgaa 
      tgggcttaa gaaattgga agtgaaatc cagagaagg 
      gtttaacgg tatgccaga atcgcctta aaggttaag 
      gttaagatc caatcaact tacgatttt ttgactaac 
      atatttttc ttaagttat agttaaaat tgtattggt 
      tagaaaaat agagcatcg gttaaatgg aagcagttg 
      tgacaggta agtacgaaa aattccgac ggcaaaatt 
      attaactac aaaaaaaat cctgacctg gattttatt 
      gatttttaa aggagttac catattttc cttgataat 
      ttcatatac gcgttgagg aaaataaac taatcttca 
      cgaatatga caattacat taaattatt tcttaaatt 
      ctgtatttt gaacctatt ttctatctc gatgtaaag 
      gttccaact cgaatttta agtgtttaa ttttttttt 
      ctattttcg attcaagct aagactgtt ctgctatct 
      ggtgttgaa cattgcaga cagaagcgt aattatttt 
      gcgacattc gttgaagat aaacacaac atctgtaaa 
      taatgtcaa ggatatttt taccgattt gaagctgaa 
      aactagttt ttgtgagat tctgttaaa tctgcctaa 
      aataatgta agctaaccg gaatatgac ataattttt 
      tcttctttc gacgaccta atacattaa ttaacgtaa 
      t
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