Status
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Gene information
General information
TC010481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451027.1: 16015..40495
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010481
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06111

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010481 RA
      MSVTNVKNI VLLTFFTIS VSATGDKIP LGAIFEQGT 
      DEVQTAFKF AMLNHNQNV TARRFELQA YVDVINTAD 
      AFKLSRLIC NQFQRGVYS MLGAVSPDS FDTLHSYSN 
      TFQMPFVTP WFPEKVLAP SSGFLDYAI SMRPEYHQA 
      IIDTVRYYG WPKIIYLYD SNDGLLRLQ QIYQGLVPG 
      SESFQVSTV RRISNVTEA LQFLRGLEE QSRWEHKYV 
      VLDCSADMA KEIVVSHVR DIALGKRTY HYLLSGLVM 
      DDRWESEVI EYGAINITG FRIVDSSRK HVKDFLDNW 
      KKLDSTGSQ NTGRESISA QAALMYDAV FVLVEAFNK 
      LLRKKQDIF RNNMRRGQI FNNGSKGLD CNASGGWVI 
      PWEHGDKIS RYLRKVEIE GLTGEIRFS EDGRRQNYT 
      LHVVEMTIN SAMVKVAEW SDETGFTPV AAKYIRLKS 
      NAQIERNRT YIVTTIVEE PYIMLRSPE PGETLSGND 
      RFEGYCKDL ADLIAKHLG ITYELRVVK DGNYGSENH 
      EVKGNWDGM VGELVRNEA DIAIAPMTI TSERERVID 
      FSKPFMSLG ISIMIKKPM KQKPGVFSF LNPLSKEIW 
      VCVIFSYIG VSIVLFTVS RFSPYEWRL LHLTGEHRD 
      PSGQHSTHN SMANDFTML NSLWFSLGA FMQQGCDIA 
      PRSISGRIV GAVWWFFTL ILISSYTAN LAAFLTVER 
      MVAPINSPE DLASQTEVE YGTLYHGAT WDFFKRSQI 
      TLYSKMWEY MNSRKHVFV KSYDEGIRR VRTSKGKYA 
      LLIESPKND YINEREPCD TMKVGRNLD AKGFGVATP 
      LGSPLRDAI NLAVLNLKE NGELTKLMN RWWYDRTEC 
      IHDKQDAAR NELSLSNVA GIFYILIGG LMIALAVAL 
      IEFCYKSHT EAVRAKIPL SDAMKAKAR LTIGVGRDI 
      DNGRYYTPA NQIAGANEQ EQAHSNTHT QV
    CDS sequence
    • >TC010481 RA
      atgagcgtc accaacgtc aaaaatatt gttttgttg 
      acgtttttc actattagt gtatcggcc actggagat 
      aaaattccc ttaggtgca atattcgag caaggcact 
      gatgaggtt caaactgca ttcaagttt gcaatgtta 
      aaccacaac cagaacgtc acggcaaga cgtttcgaa 
      ctacaagcg tacgtcgac gtcataaat acagcagac 
      gctttcaaa ttatcaaga cttatatgt aaccagttc 
      cagagaggc gtttactca atgttggga gctgtttca 
      ccagattcc ttcgacacg ttacattcc tacagcaac 
      actttccaa atgcccttt gtcacccca tggttccct 
      gaaaaagtc ctagcccca tcatcgggt ttcctggat 
      tatgccatt tccatgcgg cctgagtac caccaggct 
      attatagac acagtcaga tattatggc tggcctaaa 
      attatttac ctttatgat tcaaatgac ggtctcctc 
      agattacag caaatttat cagggttta gtgccggga 
      agtgagagt tttcaagtg tcaacagtt agaagaata 
      agcaatgtt acggaagct ttacagttc ctgagagga 
      ctggaggaa cagtcgcga tgggagcat aaatacgta 
      gtgctcgat tgttctgca gatatggcg aaagaaatt 
      gtcgttagt cacgttagg gatattgcg ttgggaaaa 
      aggacctat cactactta ctgtctggt ttagtgatg 
      gacgataga tgggagagt gaagtaatc gaatacggc 
      gcaattaac ataactgga tttcgaata gttgatagt 
      tccagaaag cacgtcaaa gactttctg gataactgg 
      aaaaagtta gattctact ggaagtcaa aatactggc 
      cgggaaagt atctcggct caagctgct cttatgtac 
      gatgctgtt tttgtcctc gttgaagct tttaataaa 
      ttattgaga aaaaaacaa gacatattt aggaataat 
      atgagacga ggacaaatt tttaataat gggagcaag 
      ggtttagac tgtaatgcg tccggaggg tgggttata 
      ccatgggag catggcgat aaaatttcg agatattta 
      agaaaggta gagattgaa ggattgact ggagagatc 
      cgttttagt gaagacggc cgacgacaa aattacact 
      cttcatgtt gtcgaaatg accataaac agtgctatg 
      gtcaaagta gcagaatgg agcgacgag actggtttt 
      acaccagtt gctgctaaa tacattcgg ctaaaaagt 
      aacgctcaa attgagaga aatcggact tatattgtt 
      acaactatt gtagaagag ccttacata atgttacgc 
      tcacctgaa cctggagag actttaagt ggaaatgat 
      cgttttgag ggctattgc aaggatttg gcagacttg 
      attgccaaa catttggga attacgtac gagctccga 
      gtggtaaaa gacggcaat tacggctca gaaaaccac 
      gaagtgaaa ggcaactgg gacggaatg gtcggagaa 
      ctagttcgc aacgaagcc gacattgcc atcgccccc 
      atgacaata acctccgaa cgcgaacgc gtgatcgac 
      ttcagcaaa ccgttcatg tcgctcgga atctcaata 
      atgatcaaa aaaccaatg aagcaaaaa ccgggagtt 
      ttcagtttc ctgaaccca ctgtcaaaa gaaatttgg 
      gtttgcgtg attttcagt tacatagga gttagtata 
      gtcctgttt acggtgtcg agattttct ccgtatgaa 
      tggagactc ctccaccta actggggaa catagagat 
      ccctcagga caacactca acacacaat tccatggct 
      aatgatttt acaatgttg aactcgttg tggttttcg 
      ttgggtgct tttatgcaa caagggtgc gatattgct 
      cccaggtct atttcggga aggattgtg ggggcggtt 
      tggtggttt tttaccctt atattaatt tcgtcgtac 
      actgccaac ttggcagcg tttctcaca gttgagagg 
      atggtggcg ccgattaat tcaccagag gatttggcc 
      tcccagacg gaggttgag tatgggaca ctgtaccat 
      ggggcgacg tgggacttt ttcaagagg tcacaaata 
      accctctac tcaaaaatg tgggaatac atgaattcg 
      cggaagcac gttttcgtc aaatcgtac gatgaaggc 
      attagacgt gttcggacc tcaaagggc aaatacgcc 
      cttttgatc gaatcgccc aaaaatgac tacatcaac 
      gagagagaa ccttgcgat acgatgaaa gttggacga 
      aatttggat gctaaaggg tttggagtt gctacacct 
      ttgggctcg cctttgaga gacgctatt aatctagca 
      gtactgaac ttgaaagag aacggcgaa ctaacaaaa 
      ctaatgaat cggtggtgg tacgacaga actgagtgc 
      attcacgac aaacaggac gcagctaga aacgagtta 
      tctctaagt aacgtcgct ggaattttc tacatactg 
      atcggaggt cttatgata gcactggct gttgctctg 
      atcgaattt tgctacaaa tcgcataca gaagccgtg 
      agagcgaaa attccgctc agtgatgcc atgaaggcg 
      aaagctcgt ttgacgatt ggcgtcgga agggatatt 
      gataatgga aggtactac actccagcc aaccaaatc 
      gccggtgct aacgaacag gagcaagcc cacagcaac 
      actcacact caagtgtga
    mRNA sequence
    • >TC010481 RA
      ctgctgata cctattatt agcttaaaa gcacattat 
      acccacaat aattaatat tataacatt agtgaagct 
      gggcgagcg ccatgagcg tcaccaacg tcaaaaata 
      ttgttttgt tgacgtttt tcactatta gtgtatcgg 
      ccactggag ataaaattc ccttaggtg caatattcg 
      agcaaggca ctgatgagg ttcaaactg cattcaagt 
      ttgcaatgt taaaccaca accagaacg tcacggcaa 
      gacgtttcg aactacaag cgtacgtcg acgtcataa 
      atacagcag acgctttca aattatcaa gacttatat 
      gtaaccagt tccagagag gcgtttact caatgttgg 
      gagctgttt caccagatt ccttcgaca cgttacatt 
      cctacagca acactttcc aaatgccct ttgtcaccc 
      catggttcc ctgaaaaag tcctagccc catcatcgg 
      gtttcctgg attatgcca tttccatgc ggcctgagt 
      accaccagg ctattatag acacagtca gatattatg 
      gctggccta aaattattt acctttatg attcaaatg 
      acggtctcc tcagattac agcaaattt atcagggtt 
      tagtgccgg gaagtgaga gttttcaag tgtcaacag 
      ttagaagaa taagcaatg ttacggaag ctttacagt 
      tcctgagag gactggagg aacagtcgc gatgggagc 
      ataaatacg tagtgctcg attgttctg cagatatgg 
      cgaaagaaa ttgtcgtta gtcacgtta gggatattg 
      cgttgggaa aaaggacct atcactact tactgtctg 
      gtttagtga tggacgata gatgggaga gtgaagtaa 
      tcgaatacg gcgcaatta acataactg gatttcgaa 
      tagttgata gttccagaa agcacgtca aagactttc 
      tggataact ggaaaaagt tagattcta ctggaagtc 
      aaaatactg gccgggaaa gtatctcgg ctcaagctg 
      ctcttatgt acgatgctg tttttgtcc tcgttgaag 
      cttttaata aattattga gaaaaaaac aagacatat 
      ttaggaata atatgagac gaggacaaa tttttaata 
      atgggagca agggtttag actgtaatg cgtccggag 
      ggtgggtta taccatggg agcatggcg ataaaattt 
      cgagatatt taagaaagg tagagattg aaggattga 
      ctggagaga tccgtttta gtgaagacg gccgacgac 
      aaaattaca ctcttcatg ttgtcgaaa tgaccataa 
      acagtgcta tggtcaaag tagcagaat ggagcgacg 
      agactggtt ttacaccag ttgctgcta aatacattc 
      ggctaaaaa gtaacgctc aaattgaga gaaatcgga 
      cttatattg ttacaacta ttgtagaag agccttaca 
      taatgttac gctcacctg aacctggag agactttaa 
      gtggaaatg atcgttttg agggctatt gcaaggatt 
      tggcagact tgattgcca aacatttgg gaattacgt 
      acgagctcc gagtggtaa aagacggca attacggct 
      cagaaaacc acgaagtga aaggcaact gggacggaa 
      tggtcggag aactagttc gcaacgaag ccgacattg 
      ccatcgccc ccatgacaa taacctccg aacgcgaac 
      gcgtgatcg acttcagca aaccgttca tgtcgctcg 
      gaatctcaa taatgatca aaaaaccaa tgaagcaaa 
      aaccgggag ttttcagtt tcctgaacc cactgtcaa 
      aagaaattt gggtttgcg tgattttca gttacatag 
      gagttagta tagtcctgt ttacggtgt cgagatttt 
      ctccgtatg aatggagac tcctccacc taactgggg 
      aacatagag atccctcag gacaacact caacacaca 
      attccatgg ctaatgatt ttacaatgt tgaactcgt 
      tgtggtttt cgttgggtg cttttatgc aacaagggt 
      gcgatattg ctcccaggt ctatttcgg gaaggattg 
      tgggggcgg tttggtggt tttttaccc ttatattaa 
      tttcgtcgt acactgcca acttggcag cgtttctca 
      cagttgaga ggatggtgg cgccgatta attcaccag 
      aggatttgg cctcccaga cggaggttg agtatggga 
      cactgtacc atggggcga cgtgggact ttttcaaga 
      ggtcacaaa taaccctct actcaaaaa tgtgggaat 
      acatgaatt cgcggaagc acgttttcg tcaaatcgt 
      acgatgaag gcattagac gtgttcgga cctcaaagg 
      gcaaatacg cccttttga tcgaatcgc ccaaaaatg 
      actacatca acgagagag aaccttgcg atacgatga 
      aagttggac gaaatttgg atgctaaag ggtttggag 
      ttgctacac ctttgggct cgcctttga gagacgcta 
      ttaatctag cagtactga acttgaaag agaacggcg 
      aactaacaa aactaatga atcggtggt ggtacgaca 
      gaactgagt gcattcacg acaaacagg acgcagcta 
      gaaacgagt tatctctaa gtaacgtcg ctggaattt 
      tctacatac tgatcggag gtcttatga tagcactgg 
      ctgttgctc tgatcgaat tttgctaca aatcgcata 
      cagaagccg tgagagcga aaattccgc tcagtgatg 
      ccatgaagg cgaaagctc gtttgacga ttggcgtcg 
      gaagggata ttgataatg gaaggtact acactccag 
      ccaaccaaa tcgccggtg ctaacgaac aggagcaag 
      cccacagca acactcaca ctcaagtgt gaagcccaa 
      aaataccaa tttgtaaga gttgacgtt ttcatcgtt 
      accatatct catcgcgtg cgtcacatt aattgtaat 
      atttttact taaacgtag attgtgtca atttagact 
      gtggctatt aaatattac ctgtttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY