Status
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Gene information
General information
TC001888 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 559322..567715
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001888
    iBeetle screen iB_06108

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001888 RA
      MNTSNGNQA NSEEIAMVV GSPMSIQSD DDDDNCQSS 
      AGSACEEAS DFMTAAMDD EVTAQLAAA GPVGVAAAA 
      AIVSAKKRK RPHSFETNP SIRKRQQNR LLRKLRQTI 
      DEFATRVGQ QAIVLVATP GKPNVSYKV FGAKPLEDV 
      IKNLRGMIM EELENALAH QAPPPVQDD PSLFELPPL 
      IIDGIPTPV EKMTQAQLR AFIPLMLKY STGRGKPGW 
      GRESTRPPW WPKELPWAN VRMDARSED EKQKISWTH 
      ALRQIVINC YKFHGREDL LPAFTEEDE KANATATAN 
      ANVSTSVGV LKMHSSGIV PTHKIQIQA NGSPQIIAA 
      SPDGLSGSS AQITTQYAP TVLHTITNP DGTVSIVQV 
      DPNNPIITL PDGTTAHVQ GMATIQTGQ GDGTPVHTI 
      QAISESGSQ GEVVDLNSV TEATLNSEG QIILTGEDG 
      HGYPISVSG VITVPVTAS MYHTMVANI QHLHTNSDG 
      TVCVTPVVP VPKSVDKVE PNNGDNMET LAVTPSGLT 
      THSMMIQSS GTEGPQVLQ VLSLKDATV LTKAMQGIA 
      DVKTEETIL SDQ
    • >TC001888 RB
      MNTSNGNQA NSEEIAMVV GSPMSIQSD DDDDNCQSS 
      AGSACEEAS DFMTAAMDD EVTAQLAAA GPVGVAAAA 
      AIVSAKKRK RPHSFETNP SIRKRQQNR LLRKLRQTI 
      DEFATRVGQ QAIVLVATP GKPNVSYKV FGAKPLEDV 
      IKNLRGMIM EELENALAH QAPPPVQDD PSLFELPPL 
      IIDGIPTPV EKMTQAQLR AFIPLMLKY STGRGKPGW 
      GRESTRPPW WPKELPWAN VRMDARSED EKQKISWTH 
      ALRQIVINC YKFHGREDL LPAFTEEDE KANATATAN 
      ANVSTSVGV LKMHSSGIV PTHKIQIQA NGSPQIIAA 
      SPDGLSGSS AQITTQYAP TVLHTITNP DGTVSIVQV 
      DPNNPIITL PDGTTAHVQ GMATIQTGQ GDGTPVHTI 
      QAISESGSQ GEVVDLNSV TEATLNSEG QIILTGEDG 
      HVSGVITVP VTASMYHTM VANIQHLHT NSDGTVCVT 
      PVVPVPKVE PNNGDNMET LAVTPSGLT THSMMIQSS 
      GTEGPQVLQ VLSLKDATV LTKAMQGIA DVKTEETIL 
      SDQ
    • >TC001888 RC
      MNTSNGNQA NSEEIAMVV GSPMSIQSD DDDDNCQSS 
      AGSACEEAS DFMTAAMDD EVTAQLAAA GPVGVAAAA 
      AIVSAKKRK RPHSFETNP SIRKRQQNR LLRKLRQTI 
      DEFATRVGQ QAIVLVATP GKPNVSYKV FGAKPLEDV 
      IKNLRGMIM EELENALAH QAPPPVQDD PSLFELPPL 
      IIDGIPTPV EKMTQAQLR AFIPLMLKY STGRGKPGW 
      GRESTRPPW WPKELPWAN VRMDARSED EKQKISWTH 
      ALRQIVINC YKFHGREDL LPAFTEEDE KANATATAN 
      ANVSTSVGV LKMHSSGIV PTHKIQIQA NGSPQIIAA 
      SPDGLSGSS AQITTQYAP TVLHTITNP DGTVSIVQV 
      DPNNPIITL PDGTTAHVQ GMATIQTGQ GDGTPVHTI 
      QAISESGSQ GEVVDLNSV TEATLNSEG QIILTGEDG 
      HGYPISVSG VITVPVTAS MYHTMVANI QHLHTNSDG 
      TVCVTPVVP VPKVEPNNG DNMETLAVT PSGLTTHSM 
      MIQSSGTEG PQVLQVLSL KDATVLTKA MQGIADVKT 
      EETILSDQ
    CDS sequence
    • >TC001888 RA
      atgaatacg tctaatggc aatcaggcc aatagtgaa 
      gaaatcgca atggttgtt ggttccccg atgtcaatc 
      cagtccgac gatgacgac gataattgt cagtcgtcg 
      gcgggctcc gcctgcgag gaggcctcc gatttcatg 
      acggcggcg atggacgat gaagtcacc gcccagtta 
      gctgctgcc ggtcctgtt ggagttgcc gccgccgcc 
      gcaatagtc tcggcgaaa aagcgcaaa aggccgcac 
      tcattcgag acaaaccct tcgatacgg aagcgacag 
      caaaatcgt ctgctccgg aagctgagg caaaccata 
      gacgaattt gcgacgcgt gtcggccag caagctata 
      gtattagtg gcgacgccg ggcaagccc aacgttagt 
      tataaggtg tttggcgcg aagccgctc gaagatgta 
      ataaaaaat ctccgaggc atgataatg gaggagtta 
      gagaacgca ttggcccac caagcccct ccgcctgtg 
      caggacgat ccgtctctg ttcgaactg ccgccactg 
      atcatcgac ggcatccca acccccgtt gaaaaaatg 
      acacaagca cagctgcgt gcgtttatc ccgcttatg 
      ctgaagtac tccactggg aggggcaaa ccggggtgg 
      gggcgcgag tcgacaagg cccccgtgg tggccgaag 
      gagctcccg tgggcgaac gtaaggatg gacgctagg 
      agcgaagac gaaaaacaa aaaatatct tggacgcac 
      gccttgcgg cagatagtc attaattgt tacaaattt 
      cacggcagg gaagatctg ttaccggcg tttacggaa 
      gaagacgag aaggccaat gccacggcg acagccaac 
      gcgaatgtc agtacatcg gttggtgtt ttaaaaatg 
      cattcctca gggattgta cctactcat aagatacag 
      atccaagct aacggctcg ccccaaatt attgccgcc 
      tcgcctgat ggcttgtcg gggtcttct gcgcagatt 
      acaacccaa tacgccccg acggtactc cacacaatt 
      acaaacccc gacggcact gtcagtatt gttcaagtg 
      gatccgaac aatcccatc attacttta ccagatggc 
      accacagct cacgttcaa ggaatggcc actatccag 
      acggggcag ggcgacggc acgcccgtg cacacgatc 
      caagcaata tccgagagt gggtctcag ggtgaagtc 
      gttgatttg aattcggtg actgaagcg acgctgaat 
      tcggaaggg cagattata ttaacgggc gaggacggg 
      cacggttac ccaatttca gtaagtggc gtgataacc 
      gtacctgta accgcgtcg atgtaccac accatggta 
      gcaaatata cagcaccta catacaaat agcgacggt 
      acagtttgt gtgactccg gtcgtccca gtaccaaag 
      tctgttgat aaggtcgag cccaataat ggagacaac 
      atggagaca ctggcggtg actcctagc gggttgact 
      actcattct atgatgatt caaagttca ggtacggag 
      ggtccacaa gttttacaa gttctctcg ttgaaggat 
      gcaactgtt ttaacaaaa gcgatgcaa ggaattgcc 
      gatgttaaa acagaagaa acaatatta agtgaccag 
      tag
    • >TC001888 RB
      atgaatacg tctaatggc aatcaggcc aatagtgaa 
      gaaatcgca atggttgtt ggttccccg atgtcaatc 
      cagtccgac gatgacgac gataattgt cagtcgtcg 
      gcgggctcc gcctgcgag gaggcctcc gatttcatg 
      acggcggcg atggacgat gaagtcacc gcccagtta 
      gctgctgcc ggtcctgtt ggagttgcc gccgccgcc 
      gcaatagtc tcggcgaaa aagcgcaaa aggccgcac 
      tcattcgag acaaaccct tcgatacgg aagcgacag 
      caaaatcgt ctgctccgg aagctgagg caaaccata 
      gacgaattt gcgacgcgt gtcggccag caagctata 
      gtattagtg gcgacgccg ggcaagccc aacgttagt 
      tataaggtg tttggcgcg aagccgctc gaagatgta 
      ataaaaaat ctccgaggc atgataatg gaggagtta 
      gagaacgca ttggcccac caagcccct ccgcctgtg 
      caggacgat ccgtctctg ttcgaactg ccgccactg 
      atcatcgac ggcatccca acccccgtt gaaaaaatg 
      acacaagca cagctgcgt gcgtttatc ccgcttatg 
      ctgaagtac tccactggg aggggcaaa ccggggtgg 
      gggcgcgag tcgacaagg cccccgtgg tggccgaag 
      gagctcccg tgggcgaac gtaaggatg gacgctagg 
      agcgaagac gaaaaacaa aaaatatct tggacgcac 
      gccttgcgg cagatagtc attaattgt tacaaattt 
      cacggcagg gaagatctg ttaccggcg tttacggaa 
      gaagacgag aaggccaat gccacggcg acagccaac 
      gcgaatgtc agtacatcg gttggtgtt ttaaaaatg 
      cattcctca gggattgta cctactcat aagatacag 
      atccaagct aacggctcg ccccaaatt attgccgcc 
      tcgcctgat ggcttgtcg gggtcttct gcgcagatt 
      acaacccaa tacgccccg acggtactc cacacaatt 
      acaaacccc gacggcact gtcagtatt gttcaagtg 
      gatccgaac aatcccatc attacttta ccagatggc 
      accacagct cacgttcaa ggaatggcc actatccag 
      acggggcag ggcgacggc acgcccgtg cacacgatc 
      caagcaata tccgagagt gggtctcag ggtgaagtc 
      gttgatttg aattcggtg actgaagcg acgctgaat 
      tcggaaggg cagattata ttaacgggc gaggacggg 
      cacgtaagt ggcgtgata accgtacct gtaaccgcg 
      tcgatgtac cacaccatg gtagcaaat atacagcac 
      ctacataca aatagcgac ggtacagtt tgtgtgact 
      ccggtcgtc ccagtacca aaggtcgag cccaataat 
      ggagacaac atggagaca ctggcggtg actcctagc 
      gggttgact actcattct atgatgatt caaagttca 
      ggtacggag ggtccacaa gttttacaa gttctctcg 
      ttgaaggat gcaactgtt ttaacaaaa gcgatgcaa 
      ggaattgcc gatgttaaa acagaagaa acaatatta 
      agtgaccag tag
    • >TC001888 RC
      atgaatacg tctaatggc aatcaggcc aatagtgaa 
      gaaatcgca atggttgtt ggttccccg atgtcaatc 
      cagtccgac gatgacgac gataattgt cagtcgtcg 
      gcgggctcc gcctgcgag gaggcctcc gatttcatg 
      acggcggcg atggacgat gaagtcacc gcccagtta 
      gctgctgcc ggtcctgtt ggagttgcc gccgccgcc 
      gcaatagtc tcggcgaaa aagcgcaaa aggccgcac 
      tcattcgag acaaaccct tcgatacgg aagcgacag 
      caaaatcgt ctgctccgg aagctgagg caaaccata 
      gacgaattt gcgacgcgt gtcggccag caagctata 
      gtattagtg gcgacgccg ggcaagccc aacgttagt 
      tataaggtg tttggcgcg aagccgctc gaagatgta 
      ataaaaaat ctccgaggc atgataatg gaggagtta 
      gagaacgca ttggcccac caagcccct ccgcctgtg 
      caggacgat ccgtctctg ttcgaactg ccgccactg 
      atcatcgac ggcatccca acccccgtt gaaaaaatg 
      acacaagca cagctgcgt gcgtttatc ccgcttatg 
      ctgaagtac tccactggg aggggcaaa ccggggtgg 
      gggcgcgag tcgacaagg cccccgtgg tggccgaag 
      gagctcccg tgggcgaac gtaaggatg gacgctagg 
      agcgaagac gaaaaacaa aaaatatct tggacgcac 
      gccttgcgg cagatagtc attaattgt tacaaattt 
      cacggcagg gaagatctg ttaccggcg tttacggaa 
      gaagacgag aaggccaat gccacggcg acagccaac 
      gcgaatgtc agtacatcg gttggtgtt ttaaaaatg 
      cattcctca gggattgta cctactcat aagatacag 
      atccaagct aacggctcg ccccaaatt attgccgcc 
      tcgcctgat ggcttgtcg gggtcttct gcgcagatt 
      acaacccaa tacgccccg acggtactc cacacaatt 
      acaaacccc gacggcact gtcagtatt gttcaagtg 
      gatccgaac aatcccatc attacttta ccagatggc 
      accacagct cacgttcaa ggaatggcc actatccag 
      acggggcag ggcgacggc acgcccgtg cacacgatc 
      caagcaata tccgagagt gggtctcag ggtgaagtc 
      gttgatttg aattcggtg actgaagcg acgctgaat 
      tcggaaggg cagattata ttaacgggc gaggacggg 
      cacggttac ccaatttca gtaagtggc gtgataacc 
      gtacctgta accgcgtcg atgtaccac accatggta 
      gcaaatata cagcaccta catacaaat agcgacggt 
      acagtttgt gtgactccg gtcgtccca gtaccaaag 
      gtcgagccc aataatgga gacaacatg gagacactg 
      gcggtgact cctagcggg ttgactact cattctatg 
      atgattcaa agttcaggt acggagggt ccacaagtt 
      ttacaagtt ctctcgttg aaggatgca actgtttta 
      acaaaagcg atgcaagga attgccgat gttaaaaca 
      gaagaaaca atattaagt gaccagtag
    mRNA sequence
    • >TC001888 RA
      atgtgtgac tgaaatcta tataaaatc gttacgtaa 
      tgctggtta aatatataa attgtggtc ggtctcttt 
      aatcttatt atttgataa taaatataa agccgtaca 
      ttggtggga agtgagatt ttgactaat ttttagatg 
      cgtcatctc aaaccttga cgcgcgcca ctgattatt 
      cgagaattt tccacactg gaagaacca agctgttgg 
      tgctttaaa gttgtaata actactgtt tacttattg 
      aaaacgatg tttgtatcg ttttcgaac catttacaa 
      ctaatcgat tggaataaa ataaataaa taattctag 
      tggagtttt aattttcct ctaaaatgt ggtgtgcta 
      ctggcgaat aggtgtcgc cacaagaca agcttgctc 
      gactaattc aaaatgata caaaacact ctaaaagtg 
      agcttgtgg ggatgcaag gtgagtaat tacgttaag 
      ggaaggttt tggagtaac gtggtggtg aaaaacagg 
      gcaaaaagg gttaaattc gtggtgaat tttcatatt 
      aaacttccc tcaattgct agtgcgcaa acgcagtag 
      cgatgtttg tggtgaaaa caatgtcgg gcattacca 
      acactgact gctacgatc gatatcaga gaatgtgac 
      gtaaattta tctgtcatt gtgcaaggc gattattta 
      ccgatgtaa aaatgttgt actaagtgt tgtggacgt 
      ggcaagtgc ggcaagaaa taaataatt gtgaaaaat 
      gcggtaaca gacatgttt ttctgttaa atgtgtaac 
      atacacgca ggaggcccg tcttccgca ccgcgggta 
      aatggtttt gataatgaa tacgtctaa tggcaatca 
      ggccaatag tgaagaaat cgcaatggt tgttggttc 
      cccgatgtc aatccagtc cgacgatga cgacgataa 
      ttgtcagtc gtcggcggg ctccgcctg cgaggaggc 
      ctccgattt catgacggc ggcgatgga cgatgaagt 
      caccgccca gttagctgc tgccggtcc tgttggagt 
      tgccgccgc cgccgcaat agtctcggc gaaaaagcg 
      caaaaggcc gcactcatt cgagacaaa cccttcgat 
      acggaagcg acagcaaaa tcgtctgct ccggaagct 
      gaggcaaac catagacga atttgcgac gcgtgtcgg 
      ccagcaagc tatagtatt agtggcgac gccgggcaa 
      gcccaacgt tagttataa ggtgtttgg cgcgaagcc 
      gctcgaaga tgtaataaa aaatctccg aggcatgat 
      aatggagga gttagagaa cgcattggc ccaccaagc 
      ccctccgcc tgtgcagga cgatccgtc tctgttcga 
      actgccgcc actgatcat cgacggcat cccaacccc 
      cgttgaaaa aatgacaca agcacagct gcgtgcgtt 
      tatcccgct tatgctgaa gtactccac tgggagggg 
      caaaccggg gtgggggcg cgagtcgac aaggccccc 
      gtggtggcc gaaggagct cccgtgggc gaacgtaag 
      gatggacgc taggagcga agacgaaaa acaaaaaat 
      atcttggac gcacgcctt gcggcagat agtcattaa 
      ttgttacaa atttcacgg cagggaaga tctgttacc 
      ggcgtttac ggaagaaga cgagaaggc caatgccac 
      ggcgacagc caacgcgaa tgtcagtac atcggttgg 
      tgttttaaa aatgcattc ctcagggat tgtacctac 
      tcataagat acagatcca agctaacgg ctcgcccca 
      aattattgc cgcctcgcc tgatggctt gtcggggtc 
      ttctgcgca gattacaac ccaatacgc cccgacggt 
      actccacac aattacaaa ccccgacgg cactgtcag 
      tattgttca agtggatcc gaacaatcc catcattac 
      tttaccaga tggcaccac agctcacgt tcaaggaat 
      ggccactat ccagacggg gcagggcga cggcacgcc 
      cgtgcacac gatccaagc aatatccga gagtgggtc 
      tcagggtga agtcgttga tttgaattc ggtgactga 
      agcgacgct gaattcgga agggcagat tatattaac 
      gggcgagga cgggcacgg ttacccaat ttcagtaag 
      tggcgtgat aaccgtacc tgtaaccgc gtcgatgta 
      ccacaccat ggtagcaaa tatacagca cctacatac 
      aaatagcga cggtacagt ttgtgtgac tccggtcgt 
      cccagtacc aaagtctgt tgataaggt cgagcccaa 
      taatggaga caacatgga gacactggc ggtgactcc 
      tagcgggtt gactactca ttctatgat gattcaaag 
      ttcaggtac ggagggtcc acaagtttt acaagttct 
      ctcgttgaa ggatgcaac tgttttaac aaaagcgat 
      gcaaggaat tgccgatgt taaaacaga agaaacaat 
      attaagtga ccagtagag ggttcacac gatcttaaa 
      gaaattttt attgcgaaa tcattaatt tttatcaag 
      aagtattta taattttac tttttttgt atagttgtc 
      tgtattatt tatatttag aaatgaaaa ttaggtttt 
      attttttgt tccaattat tttgactgt taacaatgt 
      ggtggtcta aatcttaac catttcaac acagattgt 
      gtttctaga taagtggaa cacttataa ctacacaaa 
      aaacgtcta cttttttac aatttgcaa tcacagtac 
      ttaggtgaa tgaacacct ccacgcata aacgctaac 
      aacgatgat tgtacagta cttacaaat tcattgtat 
      gtatggccg caaagagaa accttgtat gtgatcatg 
      taacgcaaa tatatattg ttaactttt tatttaccg 
      tttattttg ttatgaaaa aatctccaa attatttcc 
      tttttacaa ccagtcgga caacattgg gtgtttcat 
      taactaatt gtttggtct tgaaatatc gtgaccaag 
      taatcaaaa aacctgccc taagccttg tgtgttaac 
      caatgatct cagttctta tcgtgcaaa ttttatatt 
      tgtcaatta tctgagaca ctactgcca tcgtggact 
      ttttatggc aagatttga cgagtgtgt gatgttgca 
      ataatttgg ctcgaaata ttgttttgt tttgttatc 
      ccacttatc taagacata acacaaaat actacgtac 
      atttactag tcaattttt ggcatagta atgatgtta 
      gttttctaa tgttgaaga gaagggtgt tgtcgtgtc 
      tgattatat tatattaga aaacgtctt tttttctta 
      tttgtcaat ctgtgttaa gttatatct ttttataca 
      tgtgcatag tatgtgtaa gtttatctt gtaaattgt 
      aataattaa tttagaata atttaatac attgttaat 
      tatttttag ttaatactg ggtatttta attgttagt 
      acacaagca ctagaaagg ttttatgtg tgtataatg 
      gtggttgtt ttggtgtgt attgtatgg ttcctttgt 
      tatttgtgc catacattt gcattgctt atttcacca 
      cttttttac ttttgctga atttccata ccttgttaa 
      tttttgtat tatataaag aaccaaata ttaattagg 
      cttggttta gaatttcgt gccaaaggc atattaaag 
      taccttcat tacaataat ttaatatta ggttacttt 
      acacaatta tttactgga ttctacgct agtaatgta 
      ataggtcaa gtctagatt aaataaccg ttcatttct 
      catattgta aaattataa acactgtac attgtatat 
      tttacaaat ctcactgta actaataac tttttgaca 
      aaataaatg taatagttt gtttcaatg tggtgtgac 
      atgttaact tttaaatgt tatttttat ggtatgcat 
      gtatattag aatttagat ctatatggc caactacct 
      gtagtggta acgaattgt agcaccata taaacatca 
      catacacta aagatatct tcctgttat cactcccag 
      ttacacggt tttgaagat atctttctt ctgtttttg 
      tttatattt aatgtgctg gcacaagag gcagatgtt 
      tgtccataa aattttcta aaaaattgt acattacac 
      ctaccaact ggccttatg ttacttata aaaatgcct 
      ttggttcta cgcactgta acgaaattt taatgatct 
      tggcctgat cattaattt cagttgtaa attttacta 
      gaattctgt aaaaccaaa taaagctat gaattatgt
    • >TC001888 RB
      atgtgtgac tgaaatcta tataaaatc gttacgtaa 
      tgctggtta aatatataa attgtggtc ggtctcttt 
      aatcttatt atttgataa taaatataa agccgtaca 
      ttggtggga agtgagatt ttgactaat ttttagatg 
      cgtcatctc aaaccttga cgcgcgcca ctgattatt 
      cgagaattt tccacactg gaagaacca agctgttgg 
      tgctttaaa gttgtaata actactgtt tacttattg 
      aaaacgatg tttgtatcg ttttcgaac catttacaa 
      ctaatcgat tggaataaa ataaataaa taattctag 
      tggagtttt aattttcct ctaaaatgt ggtgtgcta 
      ctggcgaat aggtgtcgc cacaagaca agcttgctc 
      gactaattc aaaatgata caaaacact ctaaaagtg 
      agcttgtgg ggatgcaag gtgagtaat tacgttaag 
      ggaaggttt tggagtaac gtggtggtg aaaaacagg 
      gcaaaaagg gttaaattc gtggtgaat tttcatatt 
      aaacttccc tcaattgct agtgcgcaa acgcagtag 
      cgatgtttg tggtgaaaa caatgtcgg gcattacca 
      acactgact gctacgatc gatatcaga gaatgtgac 
      gtaaattta tctgtcatt gtgcaaggc gattattta 
      ccgatgtaa aaatgttgt actaagtgt tgtggacgt 
      ggcaagtgc ggcaagaaa taaataatt gtgaaaaat 
      gcggtaaca gacatgttt ttctgttaa atgtgtaac 
      atacacgca ggaggcccg tcttccgca ccgcgggta 
      aatggtttt gataatgaa tacgtctaa tggcaatca 
      ggccaatag tgaagaaat cgcaatggt tgttggttc 
      cccgatgtc aatccagtc cgacgatga cgacgataa 
      ttgtcagtc gtcggcggg ctccgcctg cgaggaggc 
      ctccgattt catgacggc ggcgatgga cgatgaagt 
      caccgccca gttagctgc tgccggtcc tgttggagt 
      tgccgccgc cgccgcaat agtctcggc gaaaaagcg 
      caaaaggcc gcactcatt cgagacaaa cccttcgat 
      acggaagcg acagcaaaa tcgtctgct ccggaagct 
      gaggcaaac catagacga atttgcgac gcgtgtcgg 
      ccagcaagc tatagtatt agtggcgac gccgggcaa 
      gcccaacgt tagttataa ggtgtttgg cgcgaagcc 
      gctcgaaga tgtaataaa aaatctccg aggcatgat 
      aatggagga gttagagaa cgcattggc ccaccaagc 
      ccctccgcc tgtgcagga cgatccgtc tctgttcga 
      actgccgcc actgatcat cgacggcat cccaacccc 
      cgttgaaaa aatgacaca agcacagct gcgtgcgtt 
      tatcccgct tatgctgaa gtactccac tgggagggg 
      caaaccggg gtgggggcg cgagtcgac aaggccccc 
      gtggtggcc gaaggagct cccgtgggc gaacgtaag 
      gatggacgc taggagcga agacgaaaa acaaaaaat 
      atcttggac gcacgcctt gcggcagat agtcattaa 
      ttgttacaa atttcacgg cagggaaga tctgttacc 
      ggcgtttac ggaagaaga cgagaaggc caatgccac 
      ggcgacagc caacgcgaa tgtcagtac atcggttgg 
      tgttttaaa aatgcattc ctcagggat tgtacctac 
      tcataagat acagatcca agctaacgg ctcgcccca 
      aattattgc cgcctcgcc tgatggctt gtcggggtc 
      ttctgcgca gattacaac ccaatacgc cccgacggt 
      actccacac aattacaaa ccccgacgg cactgtcag 
      tattgttca agtggatcc gaacaatcc catcattac 
      tttaccaga tggcaccac agctcacgt tcaaggaat 
      ggccactat ccagacggg gcagggcga cggcacgcc 
      cgtgcacac gatccaagc aatatccga gagtgggtc 
      tcagggtga agtcgttga tttgaattc ggtgactga 
      agcgacgct gaattcgga agggcagat tatattaac 
      gggcgagga cgggcacgt aagtggcgt gataaccgt 
      acctgtaac cgcgtcgat gtaccacac catggtagc 
      aaatataca gcacctaca tacaaatag cgacggtac 
      agtttgtgt gactccggt cgtcccagt accaaaggt 
      cgagcccaa taatggaga caacatgga gacactggc 
      ggtgactcc tagcgggtt gactactca ttctatgat 
      gattcaaag ttcaggtac ggagggtcc acaagtttt 
      acaagttct ctcgttgaa ggatgcaac tgttttaac 
      aaaagcgat gcaaggaat tgccgatgt taaaacaga 
      agaaacaat attaagtga ccagtagag ggttcacac 
      gatcttaaa gaaattttt attgcgaaa tcattaatt 
      tttatcaag aagtattta taattttac tttttttgt 
      atagttgtc tgtattatt tatatttag aaatgaaaa 
      ttaggtttt attttttgt tccaattat tttgactgt 
      taacaatgt ggtggtcta aatcttaac catttcaac 
      acagattgt gtttctaga taagtggaa cacttataa 
      ctacacaaa aaacgtcta cttttttac aatttgcaa 
      tcacagtac ttaggtgaa tgaacacct ccacgcata 
      aacgctaac aacgatgat tgtacagta cttacaaat 
      tcattgtat gtatggccg caaagagaa accttgtat 
      gtgatcatg taacgcaaa tatatattg ttaactttt 
      tatttaccg tttattttg ttatgaaaa aatctccaa 
      attatttcc tttttacaa ccagtcgga caacattgg 
      gtgtttcat taactaatt gtttggtct tgaaatatc 
      gtgaccaag taatcaaaa aacctgccc taagccttg 
      tgtgttaac caatgatct cagttctta tcgtgcaaa 
      ttttatatt tgtcaatta tctgagaca ctactgcca 
      tcgtggact ttttatggc aagatttga cgagtgtgt 
      gatgttgca ataatttgg ctcgaaata ttgttttgt 
      tttgttatc ccacttatc taagacata acacaaaat 
      actacgtac atttactag tcaattttt ggcatagta 
      atgatgtta gttttctaa tgttgaaga gaagggtgt 
      tgtcgtgtc tgattatat tatattaga aaacgtctt 
      tttttctta tttgtcaat ctgtgttaa gttatatct 
      ttttataca tgtgcatag tatgtgtaa gtttatctt 
      gtaaattgt aataattaa tttagaata atttaatac 
      attgttaat tatttttag ttaatactg ggtatttta 
      attgttagt acacaagca ctagaaagg ttttatgtg 
      tgtataatg gtggttgtt ttggtgtgt attgtatgg 
      ttcctttgt tatttgtgc catacattt gcattgctt 
      atttcacca cttttttac ttttgctga atttccata 
      ccttgttaa tttttgtat tatataaag aaccaaata 
      ttaattagg cttggttta gaatttcgt gccaaaggc 
      atattaaag taccttcat tacaataat ttaatatta 
      ggttacttt acacaatta tttactgga ttctacgct 
      agtaatgta ataggtcaa gtctagatt aaataaccg 
      ttcatttct catattgta aaattataa acactgtac 
      attgtatat tttacaaat ctcactgta actaataac 
      tttttgaca aaataaatg taatagttt gtttcaatg 
      tggtgtgac atgttaact tttaaatgt tatttttat 
      ggtatgcat gtatattag aatttagat ctatatggc 
      caactacct gtagtggta acgaattgt agcaccata 
      taaacatca catacacta aagatatct tcctgttat 
      cactcccag ttacacggt tttgaagat atctttctt 
      ctgtttttg tttatattt aatgtgctg gcacaagag 
      gcagatgtt tgtccataa aattttcta aaaaattgt 
      acattacac ctaccaact ggccttatg ttacttata 
      aaaatgcct ttggttcta cgcactgta acgaaattt 
      taatgatct tggcctgat cattaattt cagttgtaa 
      attttacta gaattctgt aaaaccaaa taaagctat 
      gaattatgt
    • >TC001888 RC
      atgtgtgac tgaaatcta tataaaatc gttacgtaa 
      tgctggtta aatatataa attgtggtc ggtctcttt 
      aatcttatt atttgataa taaatataa agccgtaca 
      ttggtggga agtgagatt ttgactaat ttttagatg 
      cgtcatctc aaaccttga cgcgcgcca ctgattatt 
      cgagaattt tccacactg gaagaacca agctgttgg 
      tgctttaaa gttgtaata actactgtt tacttattg 
      aaaacgatg tttgtatcg ttttcgaac catttacaa 
      ctaatcgat tggaataaa ataaataaa taattctag 
      tggagtttt aattttcct ctaaaatgt ggtgtgcta 
      ctggcgaat aggtgtcgc cacaagaca agcttgctc 
      gactaattc aaaatgata caaaacact ctaaaagtg 
      agcttgtgg ggatgcaag gtgagtaat tacgttaag 
      ggaaggttt tggagtaac gtggtggtg aaaaacagg 
      gcaaaaagg gttaaattc gtggtgaat tttcatatt 
      aaacttccc tcaattgct agtgcgcaa acgcagtag 
      cgatgtttg tggtgaaaa caatgtcgg gcattacca 
      acactgact gctacgatc gatatcaga gaatgtgac 
      gtaaattta tctgtcatt gtgcaaggc gattattta 
      ccgatgtaa aaatgttgt actaagtgt tgtggacgt 
      ggcaagtgc ggcaagaaa taaataatt gtgaaaaat 
      gcggtaaca gacatgttt ttctgttaa atgtgtaac 
      atacacgca ggaggcccg tcttccgca ccgcgggta 
      aatggtttt gataatgaa tacgtctaa tggcaatca 
      ggccaatag tgaagaaat cgcaatggt tgttggttc 
      cccgatgtc aatccagtc cgacgatga cgacgataa 
      ttgtcagtc gtcggcggg ctccgcctg cgaggaggc 
      ctccgattt catgacggc ggcgatgga cgatgaagt 
      caccgccca gttagctgc tgccggtcc tgttggagt 
      tgccgccgc cgccgcaat agtctcggc gaaaaagcg 
      caaaaggcc gcactcatt cgagacaaa cccttcgat 
      acggaagcg acagcaaaa tcgtctgct ccggaagct 
      gaggcaaac catagacga atttgcgac gcgtgtcgg 
      ccagcaagc tatagtatt agtggcgac gccgggcaa 
      gcccaacgt tagttataa ggtgtttgg cgcgaagcc 
      gctcgaaga tgtaataaa aaatctccg aggcatgat 
      aatggagga gttagagaa cgcattggc ccaccaagc 
      ccctccgcc tgtgcagga cgatccgtc tctgttcga 
      actgccgcc actgatcat cgacggcat cccaacccc 
      cgttgaaaa aatgacaca agcacagct gcgtgcgtt 
      tatcccgct tatgctgaa gtactccac tgggagggg 
      caaaccggg gtgggggcg cgagtcgac aaggccccc 
      gtggtggcc gaaggagct cccgtgggc gaacgtaag 
      gatggacgc taggagcga agacgaaaa acaaaaaat 
      atcttggac gcacgcctt gcggcagat agtcattaa 
      ttgttacaa atttcacgg cagggaaga tctgttacc 
      ggcgtttac ggaagaaga cgagaaggc caatgccac 
      ggcgacagc caacgcgaa tgtcagtac atcggttgg 
      tgttttaaa aatgcattc ctcagggat tgtacctac 
      tcataagat acagatcca agctaacgg ctcgcccca 
      aattattgc cgcctcgcc tgatggctt gtcggggtc 
      ttctgcgca gattacaac ccaatacgc cccgacggt 
      actccacac aattacaaa ccccgacgg cactgtcag 
      tattgttca agtggatcc gaacaatcc catcattac 
      tttaccaga tggcaccac agctcacgt tcaaggaat 
      ggccactat ccagacggg gcagggcga cggcacgcc 
      cgtgcacac gatccaagc aatatccga gagtgggtc 
      tcagggtga agtcgttga tttgaattc ggtgactga 
      agcgacgct gaattcgga agggcagat tatattaac 
      gggcgagga cgggcacgg ttacccaat ttcagtaag 
      tggcgtgat aaccgtacc tgtaaccgc gtcgatgta 
      ccacaccat ggtagcaaa tatacagca cctacatac 
      aaatagcga cggtacagt ttgtgtgac tccggtcgt 
      cccagtacc aaaggtcga gcccaataa tggagacaa 
      catggagac actggcggt gactcctag cgggttgac 
      tactcattc tatgatgat tcaaagttc aggtacgga 
      gggtccaca agttttaca agttctctc gttgaagga 
      tgcaactgt tttaacaaa agcgatgca aggaattgc 
      cgatgttaa aacagaaga aacaatatt aagtgacca 
      gtagagggt tcacacgat cttaaagaa atttttatt 
      gcgaaatca ttaattttt atcaagaag tatttataa 
      ttttacttt ttttgtata gttgtctgt attatttat 
      atttagaaa tgaaaatta ggttttatt ttttgttcc 
      aattatttt gactgttaa caatgtggt ggtctaaat 
      cttaaccat ttcaacaca gattgtgtt tctagataa 
      gtggaacac ttataacta cacaaaaaa cgtctactt 
      ttttacaat ttgcaatca cagtactta ggtgaatga 
      acacctcca cgcataaac gctaacaac gatgattgt 
      acagtactt acaaattca ttgtatgta tggccgcaa 
      agagaaacc ttgtatgtg atcatgtaa cgcaaatat 
      atattgtta actttttat ttaccgttt attttgtta 
      tgaaaaaat ctccaaatt atttccttt ttacaacca 
      gtcggacaa cattgggtg tttcattaa ctaattgtt 
      tggtcttga aatatcgtg accaagtaa tcaaaaaac 
      ctgccctaa gccttgtgt gttaaccaa tgatctcag 
      ttcttatcg tgcaaattt tatatttgt caattatct 
      gagacacta ctgccatcg tggactttt tatggcaag 
      atttgacga gtgtgtgat gttgcaata atttggctc 
      gaaatattg ttttgtttt gttatccca cttatctaa 
      gacataaca caaaatact acgtacatt tactagtca 
      atttttggc atagtaatg atgttagtt ttctaatgt 
      tgaagagaa gggtgttgt cgtgtctga ttatattat 
      attagaaaa cgtcttttt ttcttattt gtcaatctg 
      tgttaagtt atatctttt tatacatgt gcatagtat 
      gtgtaagtt tatcttgta aattgtaat aattaattt 
      agaataatt taatacatt gttaattat ttttagtta 
      atactgggt attttaatt gttagtaca caagcacta 
      gaaaggttt tatgtgtgt ataatggtg gttgttttg 
      gtgtgtatt gtatggttc ctttgttat ttgtgccat 
      acatttgca ttgcttatt tcaccactt ttttacttt 
      tgctgaatt tccatacct tgttaattt ttgtattat 
      ataaagaac caaatatta attaggctt ggtttagaa 
      tttcgtgcc aaaggcata ttaaagtac cttcattac 
      aataattta atattaggt tactttaca caattattt 
      actggattc tacgctagt aatgtaata ggtcaagtc 
      tagattaaa taaccgttc atttctcat attgtaaaa 
      ttataaaca ctgtacatt gtatatttt acaaatctc 
      actgtaact aataacttt ttgacaaaa taaatgtaa 
      tagtttgtt tcaatgtgg tgtgacatg ttaactttt 
      aaatgttat ttttatggt atgcatgta tattagaat 
      ttagatcta tatggccaa ctacctgta gtggtaacg 
      aattgtagc accatataa acatcacat acactaaag 
      atatcttcc tgttatcac tcccagtta cacggtttt 
      gaagatatc tttcttctg tttttgttt atatttaat 
      gtgctggca caagaggca gatgtttgt ccataaaat 
      tttctaaaa aattgtaca ttacaccta ccaactggc 
      cttatgtta cttataaaa atgcctttg gttctacgc 
      actgtaacg aaattttaa tgatcttgg cctgatcat 
      taatttcag ttgtaaatt ttactagaa ttctgtaaa 
      accaaataa agctatgaa ttatgt
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