| General information | |||
| TC002360 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452276.1: 115283..124092 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002360 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06103 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (16 dpi)
Days post injection
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 10 (19 dpi)
Days post injection
- >TC002360 RA
MKNKPIRHK DSNTFKFQP FAERIANID IDIFHRVGH SNEADDETS ECFFHQAME NWSILNLTE GFDNFKKEI KAHTLVTLP QLILDKERV VEVLLKHLR LRNFLSLQP LLDLVVAVA KDLRKDFYQ YYKPVLEVL ISLLNTKNT EQLEWTFTC LAYILKFVW RPLLKDITT VFDTFLPLL SNSQPDYIH NFAASSFAF IARKVKDWP TFLSHVLKS VKNRKDGVD GCGKLFFEV VSGVAGQFH SCAETILPL FLESLSNAK FPQNILFEI LEKVVENIV ENIHPQKGQ LWWDCLIGR LDSLLNNPE KSAKEIELV LRLLGQSIE YRNGKFLQN NSPVIKLIF DIFNCDLPD FVLQTATKI AILLLLAKN IKLTQEQAS GITRKVLNL PQEQILLYF VSNVSEFSA FEALVLPTF LQHCVKTGL NNDSLKVLT ELVLKKAPL SNCGINLPK WGKYPLDFR NQTIETKFL NHLNFSDFH NYVAALICV PHLNLTDVS QVTGILSEN INKFLTQIE TGQSNITNV LFLLNSTVE CLIHLNEIS YLSDNFDRI FDTLAPISS LLSLKTIDF LITVLKDRP HVINMATLR KITANLVSG FSSPHHEIR LVTAHLYSL FEDLPEFDL RHSSDPDTP KEKFQIFTI CYKIESIES QVQTYRDQL QNLEKLNFD KPQMIMCLK TEFRTIPLR YLCGTLYIN FKLLWEPVV QIIEGHAHG LEIGEFWEV FGAELRTVV GRVRDEGEK GEEFDAKWE VINELWKKS GEFKSGPDY ANYRLLLWK ALGLFPDIA EAKTRDVSD LLLDFIEKE YTKNNSAVA TNWNIRQNT SQETETDEG LEDEKQETK PKKGKSNLQ VLLTCLPVF TKIRSPKSI YREPELAKL YFDFLQHKN SQVQKLALD CIMTYKHKF LTPYKDHLY NLVDDKNFK NELAAFQID KESGTIQEE HRDDLVPLI IRIVFSKMS TKTGLRTGG KSSGQLRRN LVLRFLAGC QDKEMLAFV KMAFQYYSK FLDDDPQVM VKGLESITL ESYIPPKRL QSAINLLNV ILDQFGGLM GNELLTFIL QIVLIIGGV LKIAFGQIG NVHAGYLAI LRTLRTGAI KLVERFFGQ FERYQWTNK QINAIFHIF VWPYLDKLN VEGIHSPTT LLKLFVQWG SNPRYFSLL VKHEDGRQD QYVLPHVIR LLLNPKSHI TVVNAIEEM VEKLLTLQA DEEDLQLVI PVDNLLPVP QHILDRIKL DDKLNYGSC ILLPFVSSI LEKIQRRLD GKNKNLNQR VLFILSWIS ELVWEADIS DTTLKLLLP LALKRCSTP IGEEIVVQL LTTISNLVG NVANPCAHL KQITPLFGE VSCASGRKI LVKVLKICL KEFGPDLVD GLNAFDTKW LDQPDFQRR HDAFKEVHK AIDEGRIDL DLGVLLAYN CYYLLKSEK DLSMKENST HCLKQLCPY LINKYPKQV DYILNDTIF NLVRNGMKS RNDDFRNDC ILLLGHLAK ECPDSHVVL RDLNKYTNK SDPEVDFFE NITHLQLHR HARALLKFC SITKDLTNL PNIRTLTQF ILPLASHYL CNEKYVGKN SVIDASIEC LGTICRILP WHQYEGLLK FYLRKLGRS VEYQKQLVR VTVAILDAF HYDLSKAQA GEDVALTAP KPEVEDLGP ETGLEHEDR GGDEEGEPQ NEDVAPSEE KNQSEDLDE EPLLDDGDA LKDEEEDAD DNQSVKLCE KVTVLCKST ATRVTRSIQ IVLLPQLHR SLAELTHYD TSHKVNRKK TSAEREEED LLRVPISLA LVKLLQRLP KAILEANLP GIFMKLCTF LKSHLESVR RVTRETLQK IMQTLGPDY LGLLLGEMT PLLNRGFQV HVLIFTIHA VLNCLKPMF EAYHIDKVL LTVLQLCQA DLFGSLSEE KEVAKIAVK TSEAKSTKS FDTFQILAQ YITDKCLMD LILPIKNVL QASHSYKTV YKAEECLRH VALGLVDNE FIEIKSLLI FAYGTASES IPELVAQTK PQLSDKEKQ KKQRERVDC YIIPKAPTG RSGVRNVKS SLKTNAYLL VEFGLRLCY VLLKREKLK DEAFNPFLD PFIPVFLNC LTSKHVKLS TLTLQCLTW VMKYDLPSL KDKIATISS SIFEILHKY ASAGLSKGD NFDLVVAAF KTMAVLVRD VKYHTIDKE QLKVLLLYA EQDMHDHDR QATAFNLLK AIIARKLVV PEIHEVMAK VAELSITSE LSHVRVQAR VVFHQYLLD YPLGHQVEN HVSFYLNQM SYEMQYGRE SAIEMISTL ISSFPKKNL IKYSGTLFV TLGARLVND EAPECRKQV SECLSSLLS RLGRKDCEA LYDIVLLWL KDKKISHRR LAAQLCGIF VTVEKALFE SRLPSLLPL VLKQFGLKE EEDEEAERI KDHHLFQLL QMLLKICNN CPNFFKREE IEDLSKHIQ GLLGYPHDW VRLAAAQFL GFVLAQTEI EELARLVAN GESGGSGYL TREPITAIK ALTLDLCDQ LQPGNIKAD LAEQVIKNL VFVARVLQK VTSDKINLL WLVKRMRKI VNSEIVEGS GTVLRTEVF KWIAAVVTV LDLDTITPI VTHLLAPLV REMVTVEES NAPLRHLAK EVGTLLKKR IGVETYTQT LSQLQQNLS VKRAERKRS RTQLAVTDP ESFAKKKIK RHEKKKEAK KRKLEQLKG AKRQFKRRK AVDLDPESE VM
- >TC002360 RA
atgaagaat aaaccgatt cgtcacaag gattcaaac acatttaag ttccaaccc tttgcggaa cgcattgct aacatcgac atagacatt ttccaccga gtggggcac agcaatgag gccgacgac gagacctcc gaatgcttt ttccaccaa gcgatggaa aattggagc attcttaac ctaactgag ggtttcgat aatttcaag aaggaaatc aaagctcac actttagtc acccttccc caacttatt ctggacaaa gaacgcgtc gttgaagtc cttttgaag cacttaaga ttgcgaaat ttcttatcg ttacaaccg ctacttgat cttgtcgta gcagttgcg aaagactta cgcaaagac ttttaccag tattacaag cccgtcctt gaggtttta atcagttta ttgaacaca aaaaacacg gaacaactc gaatggacg tttacttgt ctcgcttat attttaaaa tttgtgtgg cggcccctt cttaaggac atcacaacg gtgtttgac acctttctc ccactatta tcaaactct cagcctgac tatattcac aattttgcg gcaagtagt ttcgctttt attgcacga aaagtcaaa gactggccc acttttctc tcccacgtc ctcaaatcg gtgaaaaat cgaaaagac ggagtcgac ggctgcgga aaattattt tttgaggtg gtttcgggc gtcgcaggc cagtttcac agttgcgct gagacaatc ctaccacta tttctggaa agcctctca aatgcgaaa ttcccccaa aatatactt ttcgaaatt ttggaaaaa gtcgtggag aatatcgtc gaaaatatt cacccgcaa aaggggcaa ctctggtgg gattgtctg atcggccga cttgattcg ttattaaac aatccggaa aaaagcgcg aaagaaatt gagctcgtt ttgagatta ttgggtcaa agtatcgag taccgaaac ggcaaattt ttacaaaac aattccccc gtaattaaa ctaattttt gatattttt aattgtgat ttgcccgat tttgtactt cagacagca acaaaaatc gcaattcta ttattacta gcgaaaaac ataaaattg acacaagaa caagcgtcg gggattacc cgaaaagtc ttaaattta ccacaagag caaattttg ttatatttt gtgtcaaac gtttccgaa ttttcggcg tttgaagct ttggtactt cccacattt ttgcaacat tgcgtcaag actggttta aataatgac agtttgaaa gttttaacc gagttagtt ttgaaaaag gcgccactt tctaattgc ggtattaat ttacctaaa tgggggaaa taccctctc gatttccgg aatcaaacg attgaaaca aaatttctc aaccatttg aattttagt gattttcat aattatgtc gccgctttg atttgcgtg ccacatttg aatttgaca gacgtgtca caggtgact gggatttta agcgaaaat ataaataag tttttaaca caaattgaa accggacaa tcaaacata actaatgtt ttatttctg ttaaatagc acagtcgag tgtctcatt catttgaac gaaattagt tacttgagt gacaatttc gaccgcatt tttgacaca ttagcgcca atttcgagt ttactgtca ttaaaaacc atcgatttt ctcattacg gtgctcaag gaccgccca cacgtcata aacatggca acgttgcgt aaaatcacg gccaatctc gtgtcaggg tttagttcc ccccaccac gaaattcgc ctagtcacc gcccacctc tattccctc ttcgaggat ttgccagaa ttcgattta agacactcc tccgatccc gacacgccc aaagaaaag tttcaaatt tttacgatt tgttacaaa atcgaatca attgaatcg caagttcaa acatacaga gaccaattg caaaatttg gagaaacta aattttgat aaacctcaa atgattatg tgcctcaag accgaattt cgaacgatt ccgttgcgg tacttgtgc ggtacgctt tatattaat ttcaagttg ttgtgggag ccggtggtt caaataatt gagggtcat gcacacgga ttggagatt ggggagttt tgggaggtg tttggggcg gagctaagg acggtcgtg ggccgggtt agggacgag ggagagaaa ggggaggag tttgacgcg aaatgggag gtaattaat gagttgtgg aaaaagagt ggggagttc aagtcaggg cccgattat gccaattat agacttttg ttatggaag gctttgggt ttgtttccg gatatcgcg gaagccaaa actagggac gtgtcagac ttgctgcta gattttatc gaaaaggag tacactaaa aataattca gcggtggcc acgaattgg aatattaga caaaatacg agtcaggaa accgaaaca gatgaaggg ttagaggac gaaaaacag gaaactaag ccaaaaaaa gggaaatcg aacttgcag gttctgctc acgtgtcta ccagttttt actaaaatt cgaagtcca aagtcaata tatcgtgag cctgaactc gccaaactc tattttgac ttcctccaa cacaaaaat tcgcaagtc caaaaactg gccttggac tgcatcatg acgtacaaa cacaaattt ttgacaccg tataaggac cacttgtat aacttggtc gatgataaa aatttcaaa aatgagttg gcggctttc caaatcgat aaagagagt ggtactatc caagaggaa catcgtgac gatttagta ccgctgatt attcgtatt gtcttcagt aaaatgagt actaaaacc ggtttaagg accggcgga aagtcctca ggacagttg aggcgaaat ttagtgctc cggtttttg gccggttgt caagacaaa gaaatgttg gctttcgtc aaaatggcc tttcagtac tattcgaaa tttttggac gatgatcct caagttatg gttaaagga ctcgagtcc ataacttta gaaagctac attcccccg aaaagacta caaagtgca ataaacctc ctcaatgtt attctcgac cagtttggt ggtttgatg ggaaatgaa ctcctaacg ttcatactc caaatagta ttaataatt ggaggcgtc ctcaaaatc gcttttggc caaatcggt aacgtccat gcaggctat ttggccatt ttgaggact ttgaggact ggagcaatt aaactagtg gaacgtttc ttcggacaa ttcgagcgt taccaatgg actaacaaa caaattaat gcgattttc cacattttt gtttggcct tatttggac aaattgaac gtcgaggga atacatagt cccacaact ttactaaaa ctgttcgtt caatggggt tccaacccg cgatatttc tctcttttg gtcaaacat gaggatggt agacaagac cagtatgtt cttccgcac gttattcgg ttgttactc aatccaaag agtcacata actgtggtt aacgcaatt gaggagatg gtcgagaag ttgttgacg ctacaagcc gacgaagag gatttgcag cttgtaatc ccagtggat aacctccta ccagtccct caacatatc ctcgatcgg attaaatta gacgacaag ttgaactac ggaagttgc atactttta ccttttgtt tcgtcgatt ttggaaaag atccagaga cgtctagat gggaaaaac aagaatttg aatcagagg gtactcttc atcttgtcg tggatatca gagttggtg tgggaagct gatattagt gataccacc ctcaaatta ttgcttcca ctcgctttg aaacgttgc tcgactccg attggtgaa gaaatcgta gttcagtta ttgacaacc atcagcaat ctagttggc aatgttgcc aacccttgt gcacattta aaacaaata acgcctttg tttggcgaa gtcagttgt gcgtccggt cgcaaaatt ctagtcaaa gtgcttaaa atatgtttg aaagagttt ggacctgat ctggttgac ggtttgaac gcttttgac accaaatgg ttggaccaa cccgatttc caacgccgc catgatgcg tttaaagag gttcacaaa gcgatagat gagggacga attgactta gatcttggt gttttactc gcttataat tgctactat ttgctcaaa agcgagaag gacctgtcg atgaaagag aattcgacc cattgtttg aaacaactg tgcccctac ttgattaac aagtatcca aaacaggtg gattacatc ctaaatgac actattttt aatttggtt cggaacgga atgaagagc cgaaatgac gatttccgg aacgattgt atcctgtta ctagggcat ttggcaaag gaatgtccc gatagtcac gtggtgttg cgcgatttg aacaaatac accaataaa agtgacccc gaggttgat tttttcgaa aatatcacg catctgcag cttcacaga cacgcaagg gctttactc aaattttgc tcgataacg aaagatttg accaacttg cccaatatt cgcactttg actcaattt attttaccg ctggcttcg cactatttg tgtaatgag aaatacgtg ggtaaaaac agtgtcatt gacgcgtcg attgagtgc ttaggaacg atctgtagg atcttgcca tggcaccaa tacgaaggt ctcttaaaa ttctatttg cggaagctt gggagaagc gtcgagtat caaaaacag ttggttcgg gtgactgtg gccattttg gatgcgttc cattatgat ttgagcaag gcgcaagct ggggaggat gtagccttg accgcgccg aaaccggaa gttgaagat ttaggaccg gaaactggt ctagaacat gaggatagg ggtggcgat gaggaaggg gaaccacag aatgaggat gtagccccc agtgaggag aaaaatcaa agtgaagat ttagacgag gaaccactt ctagatgat ggagatgct ctaaaggat gaggaagag gacgctgat gataatcaa agtgtgaaa ctttgtgag aaagtgaca gttttgtgc aaatcaaca gctacaaga gtgactcga agcatccaa atcgtgctt ttgcctcaa ttgcataga tccttggcc gagttgact cactatgac acttcgcat aaagtcaac cgcaagaaa acaagtgcc gaacgcgag gaggaagat ttgctccga gtgcccata tctttggct ttggttaag ttgttgcaa agattaccg aaagcaata ctcgaagct aatttgcct ggtattttc atgaagctt tgtacgttt ttgaagtca cacttggag agtgtccgg cgcgttaca cgagagacg ttacaaaaa ataatgcag accttaggt cccgattat ttggggttg cttttgggc gaaatgact cctctttta aaccgtggt tttcaagtc catgtcctc atatttaca attcacgca gtcctcaat tgcctcaaa cccatgttt gaggcttac cacatcgat aaagttctt ctgacagtt ttgcagttg tgtcaagcc gacttgttt gggagcctt tccgaggag aaagaagtc gccaagatt gcagtcaag acttctgaa gccaaaagc accaaaagt ttcgacacg ttccaaatt ttggcacaa tacatcacc gacaagtgc ctcatggac ctcattcta ccaatcaaa aacgtccta caagcctca cactcgtac aaaacggtg tacaaggcc gaggagtgc ctccgtcac gtggctcta ggtctggtc gataatgag tttatcgaa atcaagtca cttctgatt tttgcttac ggtaccgca tccgaaagc attcccgaa ctagtcgcc caaaccaag ccccagttg agcgacaaa gaaaaacaa aagaagcaa cgcgagcgt gttgattgc tacatcatt cccaaagca ccgactggt cgttcgggc gttcgcaac gtcaagtcg tcactaaaa acaaacgcg tatctactg gtcgagttt ggtttgcgt ctctgctac gtcctcctg aaacgcgag aaattaaaa gacgaggcc tttaacccg ttccttgac ccctttatc ccagtcttc ctcaactgt ctcacctcc aaacacgtc aagttgagc actttgacc ctccaatgc ctcacgtgg gtcatgaag tacgactta ccctctctc aaagacaaa atcgcaacg atctcgtca agtattttc gagattttg cacaaatac gcctctgct ggactcagc aaaggcgat aatttcgac ctagtggtt gccgcgttt aagaccatg gcggtgctt gtgcgtgac gtcaagtat catactatt gataaggaa caattgaag gttttgtta ctctatgcc gagcaggat atgcacgat catgaccga caagctaca gcttttaac cttctcaag gccattatc gccaggaag ctagtcgtg ccggagata cacgaagtg atggcgaaa gtggccgag ttgagtatc acgtcggag ctgagtcac gtgagggtg caggcgagg gttgtcttt catcagtat ttgttggat tatcctttg ggccaccaa gtggaaaat catgtttcg ttctatttg aatcaaatg agctatgag atgcagtat gggcgagag tcggccatt gagatgatc agtactttg atttcttcg tttcctaag aaaaatttg attaaatac tcggggaca cttttcgtg accttaggg gcgcgtctg gtcaatgac gaggcaccg gagtgtcgc aaacaagtc tccgagtgt ctttcttca ctcttgtct cgattagga agaaaagac tgtgaggca ttatacgat attgtgctt ctctggctc aaggataag aagataagt catcgccga cttgccgct caattatgc ggaattttt gtaacagtt gagaaggct ttattcgag tcaagacta ccaagtctt cttcctcta gtcttgaaa cagttcgga ttaaaggaa gaagaagat gaagaagcg gaacgaatc aaggatcac cacctattc caattgcta caaatgttg ctcaaaatt tgtaacaac tgtccaaac ttcttcaaa cgtgaagaa atcgaagat ttgagtaaa cacatccag ggcttgctg ggatatcca cacgattgg gtgagatta gctgcggct caattcctc ggtttcgta ttggctcag accgagatt gaggagttg gcgcgttta gtagccaat ggtgagtca ggggggagt ggctacttg acgcgtgag ccaatcaca gccatcaaa gcactgacc ttggatttg tgcgaccaa ttacaaccc ggcaatatc aaggcggac ttggcggaa caagtaatc aagaatctc gttttcgta gcgcgggtt ttacaaaaa gtgacaagt gacaaaatt aatcttttg tggttggtg aagcgtatg cggaaaatc gtaaatagt gaaattgtt gagggttcg ggtaccgtt ttacgaacc gaagttttc aaatggatt gctgccgta gtgactgtg ctagatcta gacaccatt accccaatt gtgactcat ttgttggcg cctctagtg cgcgaaatg gtcacagtg gaggaaagc aacgccccc ttgagacac ctcgccaaa gaagtgggt actttactc aaaaaacgc atcggtgtc gagacctac acccaaacc ctgtcccag ctccaacag aacttgtcc gtcaagcga gccgaacgt aagcgttcg cgaacgcag ttggccgtg accgatccg gagagcttc gctaaaaag aagatcaag agacacgag aagaaaaag gaggcgaag aaacgcaaa ctggagcag ttgaaaggc gcgaaaaga cagttcaaa cggcggaaa gccgtcgat ttggatccg gaatcggaa gtcatgtga
- >TC002360 RA
taggttatg tttgaccgc agcatgttt ttattttgt tgaatatca agactagtt taatttaat ccaaccaca atgaagaat aaaccgatt cgtcacaag gattcaaac acatttaag ttccaaccc tttgcggaa cgcattgct aacatcgac atagacatt ttccaccga gtggggcac agcaatgag gccgacgac gagacctcc gaatgcttt ttccaccaa gcgatggaa aattggagc attcttaac ctaactgag ggtttcgat aatttcaag aaggaaatc aaagctcac actttagtc acccttccc caacttatt ctggacaaa gaacgcgtc gttgaagtc cttttgaag cacttaaga ttgcgaaat ttcttatcg ttacaaccg ctacttgat cttgtcgta gcagttgcg aaagactta cgcaaagac ttttaccag tattacaag cccgtcctt gaggtttta atcagttta ttgaacaca aaaaacacg gaacaactc gaatggacg tttacttgt ctcgcttat attttaaaa tttgtgtgg cggcccctt cttaaggac atcacaacg gtgtttgac acctttctc ccactatta tcaaactct cagcctgac tatattcac aattttgcg gcaagtagt ttcgctttt attgcacga aaagtcaaa gactggccc acttttctc tcccacgtc ctcaaatcg gtgaaaaat cgaaaagac ggagtcgac ggctgcgga aaattattt tttgaggtg gtttcgggc gtcgcaggc cagtttcac agttgcgct gagacaatc ctaccacta tttctggaa agcctctca aatgcgaaa ttcccccaa aatatactt ttcgaaatt ttggaaaaa gtcgtggag aatatcgtc gaaaatatt cacccgcaa aaggggcaa ctctggtgg gattgtctg atcggccga cttgattcg ttattaaac aatccggaa aaaagcgcg aaagaaatt gagctcgtt ttgagatta ttgggtcaa agtatcgag taccgaaac ggcaaattt ttacaaaac aattccccc gtaattaaa ctaattttt gatattttt aattgtgat ttgcccgat tttgtactt cagacagca acaaaaatc gcaattcta ttattacta gcgaaaaac ataaaattg acacaagaa caagcgtcg gggattacc cgaaaagtc ttaaattta ccacaagag caaattttg ttatatttt gtgtcaaac gtttccgaa ttttcggcg tttgaagct ttggtactt cccacattt ttgcaacat tgcgtcaag actggttta aataatgac agtttgaaa gttttaacc gagttagtt ttgaaaaag gcgccactt tctaattgc ggtattaat ttacctaaa tgggggaaa taccctctc gatttccgg aatcaaacg attgaaaca aaatttctc aaccatttg aattttagt gattttcat aattatgtc gccgctttg atttgcgtg ccacatttg aatttgaca gacgtgtca caggtgact gggatttta agcgaaaat ataaataag tttttaaca caaattgaa accggacaa tcaaacata actaatgtt ttatttctg ttaaatagc acagtcgag tgtctcatt catttgaac gaaattagt tacttgagt gacaatttc gaccgcatt tttgacaca ttagcgcca atttcgagt ttactgtca ttaaaaacc atcgatttt ctcattacg gtgctcaag gaccgccca cacgtcata aacatggca acgttgcgt aaaatcacg gccaatctc gtgtcaggg tttagttcc ccccaccac gaaattcgc ctagtcacc gcccacctc tattccctc ttcgaggat ttgccagaa ttcgattta agacactcc tccgatccc gacacgccc aaagaaaag tttcaaatt tttacgatt tgttacaaa atcgaatca attgaatcg caagttcaa acatacaga gaccaattg caaaatttg gagaaacta aattttgat aaacctcaa atgattatg tgcctcaag accgaattt cgaacgatt ccgttgcgg tacttgtgc ggtacgctt tatattaat ttcaagttg ttgtgggag ccggtggtt caaataatt gagggtcat gcacacgga ttggagatt ggggagttt tgggaggtg tttggggcg gagctaagg acggtcgtg ggccgggtt agggacgag ggagagaaa ggggaggag tttgacgcg aaatgggag gtaattaat gagttgtgg aaaaagagt ggggagttc aagtcaggg cccgattat gccaattat agacttttg ttatggaag gctttgggt ttgtttccg gatatcgcg gaagccaaa actagggac gtgtcagac ttgctgcta gattttatc gaaaaggag tacactaaa aataattca gcggtggcc acgaattgg aatattaga caaaatacg agtcaggaa accgaaaca gatgaaggg ttagaggac gaaaaacag gaaactaag ccaaaaaaa gggaaatcg aacttgcag gttctgctc acgtgtcta ccagttttt actaaaatt cgaagtcca aagtcaata tatcgtgag cctgaactc gccaaactc tattttgac ttcctccaa cacaaaaat tcgcaagtc caaaaactg gccttggac tgcatcatg acgtacaaa cacaaattt ttgacaccg tataaggac cacttgtat aacttggtc gatgataaa aatttcaaa aatgagttg gcggctttc caaatcgat aaagagagt ggtactatc caagaggaa catcgtgac gatttagta ccgctgatt attcgtatt gtcttcagt aaaatgagt actaaaacc ggtttaagg accggcgga aagtcctca ggacagttg aggcgaaat ttagtgctc cggtttttg gccggttgt caagacaaa gaaatgttg gctttcgtc aaaatggcc tttcagtac tattcgaaa tttttggac gatgatcct caagttatg gttaaagga ctcgagtcc ataacttta gaaagctac attcccccg aaaagacta caaagtgca ataaacctc ctcaatgtt attctcgac cagtttggt ggtttgatg ggaaatgaa ctcctaacg ttcatactc caaatagta ttaataatt ggaggcgtc ctcaaaatc gcttttggc caaatcggt aacgtccat gcaggctat ttggccatt ttgaggact ttgaggact ggagcaatt aaactagtg gaacgtttc ttcggacaa ttcgagcgt taccaatgg actaacaaa caaattaat gcgattttc cacattttt gtttggcct tatttggac aaattgaac gtcgaggga atacatagt cccacaact ttactaaaa ctgttcgtt caatggggt tccaacccg cgatatttc tctcttttg gtcaaacat gaggatggt agacaagac cagtatgtt cttccgcac gttattcgg ttgttactc aatccaaag agtcacata actgtggtt aacgcaatt gaggagatg gtcgagaag ttgttgacg ctacaagcc gacgaagag gatttgcag cttgtaatc ccagtggat aacctccta ccagtccct caacatatc ctcgatcgg attaaatta gacgacaag ttgaactac ggaagttgc atactttta ccttttgtt tcgtcgatt ttggaaaag atccagaga cgtctagat gggaaaaac aagaatttg aatcagagg gtactcttc atcttgtcg tggatatca gagttggtg tgggaagct gatattagt gataccacc ctcaaatta ttgcttcca ctcgctttg aaacgttgc tcgactccg attggtgaa gaaatcgta gttcagtta ttgacaacc atcagcaat ctagttggc aatgttgcc aacccttgt gcacattta aaacaaata acgcctttg tttggcgaa gtcagttgt gcgtccggt cgcaaaatt ctagtcaaa gtgcttaaa atatgtttg aaagagttt ggacctgat ctggttgac ggtttgaac gcttttgac accaaatgg ttggaccaa cccgatttc caacgccgc catgatgcg tttaaagag gttcacaaa gcgatagat gagggacga attgactta gatcttggt gttttactc gcttataat tgctactat ttgctcaaa agcgagaag gacctgtcg atgaaagag aattcgacc cattgtttg aaacaactg tgcccctac ttgattaac aagtatcca aaacaggtg gattacatc ctaaatgac actattttt aatttggtt cggaacgga atgaagagc cgaaatgac gatttccgg aacgattgt atcctgtta ctagggcat ttggcaaag gaatgtccc gatagtcac gtggtgttg cgcgatttg aacaaatac accaataaa agtgacccc gaggttgat tttttcgaa aatatcacg catctgcag cttcacaga cacgcaagg gctttactc aaattttgc tcgataacg aaagatttg accaacttg cccaatatt cgcactttg actcaattt attttaccg ctggcttcg cactatttg tgtaatgag aaatacgtg ggtaaaaac agtgtcatt gacgcgtcg attgagtgc ttaggaacg atctgtagg atcttgcca tggcaccaa tacgaaggt ctcttaaaa ttctatttg cggaagctt gggagaagc gtcgagtat caaaaacag ttggttcgg gtgactgtg gccattttg gatgcgttc cattatgat ttgagcaag gcgcaagct ggggaggat gtagccttg accgcgccg aaaccggaa gttgaagat ttaggaccg gaaactggt ctagaacat gaggatagg ggtggcgat gaggaaggg gaaccacag aatgaggat gtagccccc agtgaggag aaaaatcaa agtgaagat ttagacgag gaaccactt ctagatgat ggagatgct ctaaaggat gaggaagag gacgctgat gataatcaa agtgtgaaa ctttgtgag aaagtgaca gttttgtgc aaatcaaca gctacaaga gtgactcga agcatccaa atcgtgctt ttgcctcaa ttgcataga tccttggcc gagttgact cactatgac acttcgcat aaagtcaac cgcaagaaa acaagtgcc gaacgcgag gaggaagat ttgctccga gtgcccata tctttggct ttggttaag ttgttgcaa agattaccg aaagcaata ctcgaagct aatttgcct ggtattttc atgaagctt tgtacgttt ttgaagtca cacttggag agtgtccgg cgcgttaca cgagagacg ttacaaaaa ataatgcag accttaggt cccgattat ttggggttg cttttgggc gaaatgact cctctttta aaccgtggt tttcaagtc catgtcctc atatttaca attcacgca gtcctcaat tgcctcaaa cccatgttt gaggcttac cacatcgat aaagttctt ctgacagtt ttgcagttg tgtcaagcc gacttgttt gggagcctt tccgaggag aaagaagtc gccaagatt gcagtcaag acttctgaa gccaaaagc accaaaagt ttcgacacg ttccaaatt ttggcacaa tacatcacc gacaagtgc ctcatggac ctcattcta ccaatcaaa aacgtccta caagcctca cactcgtac aaaacggtg tacaaggcc gaggagtgc ctccgtcac gtggctcta ggtctggtc gataatgag tttatcgaa atcaagtca cttctgatt tttgcttac ggtaccgca tccgaaagc attcccgaa ctagtcgcc caaaccaag ccccagttg agcgacaaa gaaaaacaa aagaagcaa cgcgagcgt gttgattgc tacatcatt cccaaagca ccgactggt cgttcgggc gttcgcaac gtcaagtcg tcactaaaa acaaacgcg tatctactg gtcgagttt ggtttgcgt ctctgctac gtcctcctg aaacgcgag aaattaaaa gacgaggcc tttaacccg ttccttgac ccctttatc ccagtcttc ctcaactgt ctcacctcc aaacacgtc aagttgagc actttgacc ctccaatgc ctcacgtgg gtcatgaag tacgactta ccctctctc aaagacaaa atcgcaacg atctcgtca agtattttc gagattttg cacaaatac gcctctgct ggactcagc aaaggcgat aatttcgac ctagtggtt gccgcgttt aagaccatg gcggtgctt gtgcgtgac gtcaagtat catactatt gataaggaa caattgaag gttttgtta ctctatgcc gagcaggat atgcacgat catgaccga caagctaca gcttttaac cttctcaag gccattatc gccaggaag ctagtcgtg ccggagata cacgaagtg atggcgaaa gtggccgag ttgagtatc acgtcggag ctgagtcac gtgagggtg caggcgagg gttgtcttt catcagtat ttgttggat tatcctttg ggccaccaa gtggaaaat catgtttcg ttctatttg aatcaaatg agctatgag atgcagtat gggcgagag tcggccatt gagatgatc agtactttg atttcttcg tttcctaag aaaaatttg attaaatac tcggggaca cttttcgtg accttaggg gcgcgtctg gtcaatgac gaggcaccg gagtgtcgc aaacaagtc tccgagtgt ctttcttca ctcttgtct cgattagga agaaaagac tgtgaggca ttatacgat attgtgctt ctctggctc aaggataag aagataagt catcgccga cttgccgct caattatgc ggaattttt gtaacagtt gagaaggct ttattcgag tcaagacta ccaagtctt cttcctcta gtcttgaaa cagttcgga ttaaaggaa gaagaagat gaagaagcg gaacgaatc aaggatcac cacctattc caattgcta caaatgttg ctcaaaatt tgtaacaac tgtccaaac ttcttcaaa cgtgaagaa atcgaagat ttgagtaaa cacatccag ggcttgctg ggatatcca cacgattgg gtgagatta gctgcggct caattcctc ggtttcgta ttggctcag accgagatt gaggagttg gcgcgttta gtagccaat ggtgagtca ggggggagt ggctacttg acgcgtgag ccaatcaca gccatcaaa gcactgacc ttggatttg tgcgaccaa ttacaaccc ggcaatatc aaggcggac ttggcggaa caagtaatc aagaatctc gttttcgta gcgcgggtt ttacaaaaa gtgacaagt gacaaaatt aatcttttg tggttggtg aagcgtatg cggaaaatc gtaaatagt gaaattgtt gagggttcg ggtaccgtt ttacgaacc gaagttttc aaatggatt gctgccgta gtgactgtg ctagatcta gacaccatt accccaatt gtgactcat ttgttggcg cctctagtg cgcgaaatg gtcacagtg gaggaaagc aacgccccc ttgagacac ctcgccaaa gaagtgggt actttactc aaaaaacgc atcggtgtc gagacctac acccaaacc ctgtcccag ctccaacag aacttgtcc gtcaagcga gccgaacgt aagcgttcg cgaacgcag ttggccgtg accgatccg gagagcttc gctaaaaag aagatcaag agacacgag aagaaaaag gaggcgaag aaacgcaaa ctggagcag ttgaaaggc gcgaaaaga cagttcaaa cggcggaaa gccgtcgat ttggatccg gaatcggaa gtcatgtga cgtagccac acctctgga gaaagtttc catggccgg ctgtttagt gaacatgtt gatagtttt tttgggttt tttgctgct tgtggaggc gtacgatcc gacggatcc ggaagttag ttccgtttt gcccccgaa ggggacgtt cgaggcatt ctatccaag gggagagga agggggaag ctcaagggc ggcacattc gcacgggag tttctacaa gttcaggaa tccggcgct cgttgatgc caaaaatgc agcggcgta cgggttcag gttcgatgg gggcaggag gtttaatta cgactaaag cagcggtca ttttttgta tatattctg tataaagtt ggttaataa acttatgta atgcaa
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