Status
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Gene information
General information
TC002345 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 101794..112371
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002345
    iBeetle screen iB_06102

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002345 RA
      MPKCDVKTR YIPATFAWT LLLVATTLF FYYPARYYL 
      EDYPWVPAY QGVITFFVL ANFTLATFM DPGVIPKAP 
      PDEDREDDF RAPLYKNVE INGITVRMK CFVLMGLVA 
      LLAIPIFGL TGFHMVLVS RGRTTNEQV TGKFKGGYN 
      PFSRGCWDN CCYTQFGPQ FPSLIKPHK YNVKRKHCP 
      HGPIATITN DSQVKTYMD NSNGIRNIN SNAYNKLSP 
      GRDGCELDM EPSASQSQD CEPTPPLQR HGSKSNFFL 
      PPMEGESPR HPRGVHYPR SSPHPRPRG LDPNRSGTP 
      ESLGPQRPS PTMQQRIKA LGVPTPLAM SSPVRRSNP 
      STPTQPRRP DFIGVSANH QPGNYYDFQ AHPNSNQRP 
      AMSQGGYGS PQRRFMSEG ELVRQEAQL SYPRSNNTV 
      DNIRELANS PQRGVYMWK DTSPGYPPP QHPDFYRSN 
      PTSPTQQAY APRPYHPAV RGGVAVYPP QSPQEFGGL 
      QVHRKGGVG TPTVPDNRR RPMSFVRAL EMSDSVELT 
      APNSQCSHL PQRPSTPDR TSVYDMNYE ISV
    • >TC002345 RB
      MPKCDVKTR YIPATFAWT LLLVATTLF FYYPARYYL 
      EDYPWVPAY QGVITFFVL ANFTLATFM DPGVIPKAP 
      PDEDREDDF RAPLYKNVE INGITVRMK CVCNHCIET 
      FDHHCPWVN NCIGRRNYR FFFFFLISL SLHMISIFT 
      LSLIYILKY GDTFSNAEP IIAFVLMGL VALLAIPIF 
      GLTGFHMVL VSRGRTTNE QVTGKFKGG YNPFSRGCW 
      DNCCYTQFG PQFPSLIKP HKYNVKRKH CPHGPIATI 
      TNDSQVKTY MDNSNGIRN INSNAYNKL SPGRDGCEL 
      DMEPSASQS QDCEPTPPL QRHGSKSNF FLPPMEGES 
      PRHPRGVHY PRSSPHPRP RGLDPNRSG TPESLGPQR 
      PSPTMQQRI KALGVPTPL AMSSPVRRS NPSTPTQPR 
      RPDFIGVSA NHQPGNYYD FQAHPNSNQ RPAMSQGGY 
      GSPQRRFMS EGELVRQEA QLSYPRSNN TVDNIRELA 
      NSPQRGVYM WKDTSPGYP PPQHPDFYR SNPTSPTQQ 
      AYAPRPYHP AVRGGVAVY PPQSPQVHR KGGVGTPTV 
      PDNRRRPMS FVRALEMSD SVELTAPNS QCSHLPQRP 
      STPDRTSVY DMNYEISV
    • >TC002345 RC
      MPKCDVKTR YIPATFAWT LLLVATTLF FYYPARYYL 
      EDYPWVPAY QGVITFFVL ANFTLATFM DPGVIPKAP 
      PDEDREDDF RAPLYKNVE INGITVRMK CVCNHCIET 
      FDHHCPWVN NCIGRRNYR FFFFFLISL SLHMISIFT 
      LSLIYILKY GDTFSNAEP IIAFVLMGL VALLAIPIF 
      GLTGFHMVL VSRGRTTNE QVTGKFKGG YNPFSRGCW 
      DNCCYTQFG PQFPSLIKP HKYNVKRKH CPHGPIATI 
      TNDSQVKTY MDNSNGIRN INSNAYNKL SPGRDGCEL 
      DMEPSASQS QDCEPTPPL QRHGSKSNF FLPPMEGES 
      PRHPRGVHY PRSSPHPRP RGLDPNRSG TPESLGPQR 
      PSPTMQQRI KALGVPTPL AMSSPVRRS NPSTPTQPR 
      RPDFIGVSA NHQPGNYYD FQAHPNSNQ RPAMSQGGY 
      GSPQRRFMS EGELVRQEA QLSYPRSNN TVDNIRELA 
      NSPQRGVYM WKDTSPGYP PPQHPDFYR SNPTSPTQQ 
      AYAPRPYHP AVRGGVAVY PPQSPQEFG GLQVHRKGG 
      VGTPTVPDN RRRPMSFVR ALEMSDSVE LTAPNSQCS 
      HLPQRPSTP DRTSVYDMN YEISV
    CDS sequence
    • >TC002345 RA
      atgcccaag tgcgatgtt aaaactaga tacatacca 
      gcgactttt gcttggact ttactgttg gtagccaca 
      acgttgttc ttctactac ccagcccga tactactta 
      gaggattat ccatgggtg cccgcgtac caaggagtg 
      atcacgttc ttcgtcctc gccaatttc accctcgct 
      acgttcatg gacccgggg gtcatacca aaagcccct 
      cctgatgaa gaccgcgag gacgatttc agggccccc 
      ttgtacaag aacgtggag atcaacggg attacggtg 
      cggatgaaa tgttttgtc ttaatgggt ttggtggct 
      ttgttagct ataccaatt tttggctta acagggttt 
      catatggtt ttggtctcg agaggaagg accaccaat 
      gaacaagtc acggggaaa tttaagggc ggttacaac 
      cccttctcg aggggctgc tgggacaac tgctgctac 
      acgcagttt gggccccaa ttcccaagt ctcataaag 
      ccccacaag tacaacgtg aagcgcaag cactgcccc 
      catggcccc atcgcgacc ataacaaac gacagtcag 
      gtcaagacc tacatggat aacagcaac ggcatccgc 
      aacatcaac tccaacgcc tacaacaag ttgtcccct 
      ggtcgagac ggctgtgaa ctggacatg gagccgtcg 
      gcgtcacaa tcgcaagat tgcgagccg acgccccct 
      cttcagcga cacggctcc aaaagcaac ttttttctg 
      ccgccgatg gagggcgaa tcgccgaga cacccccgt 
      ggcgttcac tacccgcga agcagcccc catcctcgg 
      ccgcggggc ttagacccg aatcggagc ggaacgccg 
      gagagtttg gggccccaa aggccgtcg ccgacgatg 
      cagcagagg atcaaggcg ttgggagtg cccacgcct 
      ttagcaatg tctagtcct gtgaggaga tccaacccg 
      agcaccccg acccagcca cggcggccg gatttcatc 
      ggcgtctct gccaaccac cagccgggc aactactat 
      gacttccaa gcacatccc aactccaac cagcgccct 
      gccatgtct caggggggc tacggcagc cctcagcgc 
      cgtttcatg tccgagggc gagctcgtc cgccaggag 
      gcgcaactg tcctacccc cgctccaac aacacggtg 
      gataacatc cgcgagttg gccaactca ccgcagcgc 
      ggcgtctac atgtggaag gacacctcg ccggggtat 
      ccgcccccg cagcacccg gacttctac cgctccaac 
      cccaccagc cccacgcag caagcgtac gcccccagg 
      ccctaccat ccggccgtg cggggcggc gtcgccgtc 
      tacccacca cagagtccg caggagttt ggtgggttg 
      caggtgcac cggaagggg ggcgtgggg accccgaca 
      gtgccggac aaccggcgg aggcccatg tcgttcgtg 
      cgggccttg gagatgtcc gattcggtc gagttgaca 
      gcccccaat tcgcagtgt tcgcacttg ccccagcgg 
      ccctccacc cccgacagg accagtgtc tatgatatg 
      aactatgag atatcggta tag
    • >TC002345 RB
      atgcccaag tgcgatgtt aaaactaga tacatacca 
      gcgactttt gcttggact ttactgttg gtagccaca 
      acgttgttc ttctactac ccagcccga tactactta 
      gaggattat ccatgggtg cccgcgtac caaggagtg 
      atcacgttc ttcgtcctc gccaatttc accctcgct 
      acgttcatg gacccgggg gtcatacca aaagcccct 
      cctgatgaa gaccgcgag gacgatttc agggccccc 
      ttgtacaag aacgtggag atcaacggg attacggtg 
      cggatgaaa tgtgtttgt aaccactgt atagaaact 
      tttgaccac cactgtcca tgggtcaat aattgcatt 
      gggagaaga aattaccga tttttcttt ttctttctc 
      atctcactt agcttacac atgatcagt atatttact 
      ttaagccta atttacatc ctcaaatat ggtgatact 
      ttctccaac gctgaacca attattgct tttgtctta 
      atgggtttg gtggctttg ttagctata ccaattttt 
      ggcttaaca gggtttcat atggttttg gtctcgaga 
      ggaaggacc accaatgaa caagtcacg gggaaattt 
      aagggcggt tacaacccc ttctcgagg ggctgctgg 
      gacaactgc tgctacacg cagtttggg ccccaattc 
      ccaagtctc ataaagccc cacaagtac aacgtgaag 
      cgcaagcac tgcccccat ggccccatc gcgaccata 
      acaaacgac agtcaggtc aagacctac atggataac 
      agcaacggc atccgcaac atcaactcc aacgcctac 
      aacaagttg tcccctggt cgagacggc tgtgaactg 
      gacatggag ccgtcggcg tcacaatcg caagattgc 
      gagccgacg ccccctctt cagcgacac ggctccaaa 
      agcaacttt tttctgccg ccgatggag ggcgaatcg 
      ccgagacac ccccgtggc gttcactac ccgcgaagc 
      agcccccat cctcggccg cggggctta gacccgaat 
      cggagcgga acgccggag agtttgggg ccccaaagg 
      ccgtcgccg acgatgcag cagaggatc aaggcgttg 
      ggagtgccc acgccttta gcaatgtct agtcctgtg 
      aggagatcc aacccgagc accccgacc cagccacgg 
      cggccggat ttcatcggc gtctctgcc aaccaccag 
      ccgggcaac tactatgac ttccaagca catcccaac 
      tccaaccag cgccctgcc atgtctcag gggggctac 
      ggcagccct cagcgccgt ttcatgtcc gagggcgag 
      ctcgtccgc caggaggcg caactgtcc tacccccgc 
      tccaacaac acggtggat aacatccgc gagttggcc 
      aactcaccg cagcgcggc gtctacatg tggaaggac 
      acctcgccg gggtatccg cccccgcag cacccggac 
      ttctaccgc tccaacccc accagcccc acgcagcaa 
      gcgtacgcc cccaggccc taccatccg gccgtgcgg 
      ggcggcgtc gccgtctac ccaccacag agtccgcag 
      gtgcaccgg aaggggggc gtggggacc ccgacagtg 
      ccggacaac cggcggagg cccatgtcg ttcgtgcgg 
      gccttggag atgtccgat tcggtcgag ttgacagcc 
      cccaattcg cagtgttcg cacttgccc cagcggccc 
      tccaccccc gacaggacc agtgtctat gatatgaac 
      tatgagata tcggtatag
    • >TC002345 RC
      atgcccaag tgcgatgtt aaaactaga tacatacca 
      gcgactttt gcttggact ttactgttg gtagccaca 
      acgttgttc ttctactac ccagcccga tactactta 
      gaggattat ccatgggtg cccgcgtac caaggagtg 
      atcacgttc ttcgtcctc gccaatttc accctcgct 
      acgttcatg gacccgggg gtcatacca aaagcccct 
      cctgatgaa gaccgcgag gacgatttc agggccccc 
      ttgtacaag aacgtggag atcaacggg attacggtg 
      cggatgaaa tgtgtttgt aaccactgt atagaaact 
      tttgaccac cactgtcca tgggtcaat aattgcatt 
      gggagaaga aattaccga tttttcttt ttctttctc 
      atctcactt agcttacac atgatcagt atatttact 
      ttaagccta atttacatc ctcaaatat ggtgatact 
      ttctccaac gctgaacca attattgct tttgtctta 
      atgggtttg gtggctttg ttagctata ccaattttt 
      ggcttaaca gggtttcat atggttttg gtctcgaga 
      ggaaggacc accaatgaa caagtcacg gggaaattt 
      aagggcggt tacaacccc ttctcgagg ggctgctgg 
      gacaactgc tgctacacg cagtttggg ccccaattc 
      ccaagtctc ataaagccc cacaagtac aacgtgaag 
      cgcaagcac tgcccccat ggccccatc gcgaccata 
      acaaacgac agtcaggtc aagacctac atggataac 
      agcaacggc atccgcaac atcaactcc aacgcctac 
      aacaagttg tcccctggt cgagacggc tgtgaactg 
      gacatggag ccgtcggcg tcacaatcg caagattgc 
      gagccgacg ccccctctt cagcgacac ggctccaaa 
      agcaacttt tttctgccg ccgatggag ggcgaatcg 
      ccgagacac ccccgtggc gttcactac ccgcgaagc 
      agcccccat cctcggccg cggggctta gacccgaat 
      cggagcgga acgccggag agtttgggg ccccaaagg 
      ccgtcgccg acgatgcag cagaggatc aaggcgttg 
      ggagtgccc acgccttta gcaatgtct agtcctgtg 
      aggagatcc aacccgagc accccgacc cagccacgg 
      cggccggat ttcatcggc gtctctgcc aaccaccag 
      ccgggcaac tactatgac ttccaagca catcccaac 
      tccaaccag cgccctgcc atgtctcag gggggctac 
      ggcagccct cagcgccgt ttcatgtcc gagggcgag 
      ctcgtccgc caggaggcg caactgtcc tacccccgc 
      tccaacaac acggtggat aacatccgc gagttggcc 
      aactcaccg cagcgcggc gtctacatg tggaaggac 
      acctcgccg gggtatccg cccccgcag cacccggac 
      ttctaccgc tccaacccc accagcccc acgcagcaa 
      gcgtacgcc cccaggccc taccatccg gccgtgcgg 
      ggcggcgtc gccgtctac ccaccacag agtccgcag 
      gagtttggt gggttgcag gtgcaccgg aaggggggc 
      gtggggacc ccgacagtg ccggacaac cggcggagg 
      cccatgtcg ttcgtgcgg gccttggag atgtccgat 
      tcggtcgag ttgacagcc cccaattcg cagtgttcg 
      cacttgccc cagcggccc tccaccccc gacaggacc 
      agtgtctat gatatgaac tatgagata tcggtatag
    mRNA sequence
    • >TC002345 RA
      agcactgcc gtccgtttc aattcaagt aattaatgt 
      gaaaaatcg atataaaag tgtgcgact tggggaatt 
      tcttttccc ccgattttg tcaagtgtc agatgagtt 
      tgacagggg caacgaacg ataacttct cccgtttgt 
      ctcagccat gttggcctg tctgaggcg aaaattcga 
      gttttgcgc gagatcttg agtaaaaaa acattatgc 
      ccaagtgcg atgttaaaa ctagataca taccagcga 
      cttttgctt ggactttac tgttggtag ccacaacgt 
      tgttcttct actacccag cccgatact acttagagg 
      attatccat gggtgcccg cgtaccaag gagtgatca 
      cgttcttcg tcctcgcca atttcaccc tcgctacgt 
      tcatggacc cgggggtca taccaaaag cccctcctg 
      atgaagacc gcgaggacg atttcaggg cccccttgt 
      acaagaacg tggagatca acgggatta cggtgcgga 
      tgaaatgtt ttgtcttaa tgggtttgg tggctttgt 
      tagctatac caatttttg gcttaacag ggtttcata 
      tggttttgg tctcgagag gaaggacca ccaatgaac 
      aagtcacgg ggaaattta agggcggtt acaacccct 
      tctcgaggg gctgctggg acaactgct gctacacgc 
      agtttgggc cccaattcc caagtctca taaagcccc 
      acaagtaca acgtgaagc gcaagcact gcccccatg 
      gccccatcg cgaccataa caaacgaca gtcaggtca 
      agacctaca tggataaca gcaacggca tccgcaaca 
      tcaactcca acgcctaca acaagttgt cccctggtc 
      gagacggct gtgaactgg acatggagc cgtcggcgt 
      cacaatcgc aagattgcg agccgacgc cccctcttc 
      agcgacacg gctccaaaa gcaactttt ttctgccgc 
      cgatggagg gcgaatcgc cgagacacc cccgtggcg 
      ttcactacc cgcgaagca gcccccatc ctcggccgc 
      ggggcttag acccgaatc ggagcggaa cgccggaga 
      gtttggggc cccaaaggc cgtcgccga cgatgcagc 
      agaggatca aggcgttgg gagtgccca cgcctttag 
      caatgtcta gtcctgtga ggagatcca acccgagca 
      ccccgaccc agccacggc ggccggatt tcatcggcg 
      tctctgcca accaccagc cgggcaact actatgact 
      tccaagcac atcccaact ccaaccagc gccctgcca 
      tgtctcagg ggggctacg gcagccctc agcgccgtt 
      tcatgtccg agggcgagc tcgtccgcc aggaggcgc 
      aactgtcct acccccgct ccaacaaca cggtggata 
      acatccgcg agttggcca actcaccgc agcgcggcg 
      tctacatgt ggaaggaca cctcgccgg ggtatccgc 
      ccccgcagc acccggact tctaccgct ccaacccca 
      ccagcccca cgcagcaag cgtacgccc ccaggccct 
      accatccgg ccgtgcggg gcggcgtcg ccgtctacc 
      caccacaga gtccgcagg agtttggtg ggttgcagg 
      tgcaccgga aggggggcg tggggaccc cgacagtgc 
      cggacaacc ggcggaggc ccatgtcgt tcgtgcggg 
      ccttggaga tgtccgatt cggtcgagt tgacagccc 
      ccaattcgc agtgttcgc acttgcccc agcggccct 
      ccacccccg acaggacca gtgtctatg atatgaact 
      atgagatat cggtatagg gcaggtgtt ggcactcac 
      gcgccgccg aatgagtgc atttagttt ctctgcatc 
      agagaccct cactattaa gtaaacatt ccgtttact 
      actcatccc tgtgtcatg gacctaaga ccaaccgta 
      gtgtgtgta atataatct atatgttat ttgttgttt 
      acaatgttt aaaaagaat tgtttaatt ttgtgtatc 
      aagaaaccg tggcttgtg ttgttgttt taccgttga 
      tttgacacg tttttattg ttttaacct gactgtaac 
      tccactaac cgaaagcag cggctcagg catcttttc 
      tacatgagt tgttgagtg tattaatgg tgtaaatat 
      atttaagtt ttagtagat tcgtccttc aatcaaacc 
      cgaatctca gcgaaagtt ttattaaac acactaaac 
      ctacattta taaaaaagg atgtaataa ataaattaa 
      tgaatcaaa gccgtctta aactgttct tgtagcctc 
      gacatcgcc tcacgagct cgaacttcc tgtatctca 
      tgcagtgcc actgcggga tctgcgcca taagttcaa 
      tcgcctgca aaaataaca ataataatt aataattac 
      aaaaaccca aactgaaat acacgaacc gtttcagga 
      ttgccaacc ggcaccgcc ccacacccc cgaaagcag 
      gcgacaacg accgccagc acgacaaac aacttaacg 
      agcttcatt ttatgcaac tgaaccgat gcactgaac 
      ccccttatt tatatgatg caaaataga tcaacctac 
      ttttcgatc gcttcaaat attactcag taacctact 
      tattaaatc ggtaatcac ccgaaaatt aggtaattt 
      tcccgcaga acttgtcgg cgctaccac tgaactaat 
      tcaattca
    • >TC002345 RB
      agcactgcc gtccgtttc aattcaagt aattaatgt 
      gaaaaatcg atataaaag tgtgcgact tggggaatt 
      tcttttccc ccgattttg tcaagtgtc agatgagtt 
      tgacagggg caacgaacg ataacttct cccgtttgt 
      ctcagccat gttggcctg tctgaggcg aaaattcga 
      gttttgcgc gagatcttg agtaaaaaa acattatgc 
      ccaagtgcg atgttaaaa ctagataca taccagcga 
      cttttgctt ggactttac tgttggtag ccacaacgt 
      tgttcttct actacccag cccgatact acttagagg 
      attatccat gggtgcccg cgtaccaag gagtgatca 
      cgttcttcg tcctcgcca atttcaccc tcgctacgt 
      tcatggacc cgggggtca taccaaaag cccctcctg 
      atgaagacc gcgaggacg atttcaggg cccccttgt 
      acaagaacg tggagatca acgggatta cggtgcgga 
      tgaaatgtg tttgtaacc actgtatag aaacttttg 
      accaccact gtccatggg tcaataatt gcattggga 
      gaagaaatt accgatttt tctttttct ttctcatct 
      cacttagct tacacatga tcagtatat ttactttaa 
      gcctaattt acatcctca aatatggtg atactttct 
      ccaacgctg aaccaatta ttgcttttg tcttaatgg 
      gtttggtgg ctttgttag ctataccaa tttttggct 
      taacagggt ttcatatgg ttttggtct cgagaggaa 
      ggaccacca atgaacaag tcacgggga aatttaagg 
      gcggttaca accccttct cgaggggct gctgggaca 
      actgctgct acacgcagt ttgggcccc aattcccaa 
      gtctcataa agccccaca agtacaacg tgaagcgca 
      agcactgcc cccatggcc ccatcgcga ccataacaa 
      acgacagtc aggtcaaga cctacatgg ataacagca 
      acggcatcc gcaacatca actccaacg cctacaaca 
      agttgtccc ctggtcgag acggctgtg aactggaca 
      tggagccgt cggcgtcac aatcgcaag attgcgagc 
      cgacgcccc ctcttcagc gacacggct ccaaaagca 
      acttttttc tgccgccga tggagggcg aatcgccga 
      gacaccccc gtggcgttc actacccgc gaagcagcc 
      cccatcctc ggccgcggg gcttagacc cgaatcgga 
      gcggaacgc cggagagtt tggggcccc aaaggccgt 
      cgccgacga tgcagcaga ggatcaagg cgttgggag 
      tgcccacgc ctttagcaa tgtctagtc ctgtgagga 
      gatccaacc cgagcaccc cgacccagc cacggcggc 
      cggatttca tcggcgtct ctgccaacc accagccgg 
      gcaactact atgacttcc aagcacatc ccaactcca 
      accagcgcc ctgccatgt ctcaggggg gctacggca 
      gccctcagc gccgtttca tgtccgagg gcgagctcg 
      tccgccagg aggcgcaac tgtcctacc cccgctcca 
      acaacacgg tggataaca tccgcgagt tggccaact 
      caccgcagc gcggcgtct acatgtgga aggacacct 
      cgccggggt atccgcccc cgcagcacc cggacttct 
      accgctcca accccacca gccccacgc agcaagcgt 
      acgccccca ggccctacc atccggccg tgcggggcg 
      gcgtcgccg tctacccac cacagagtc cgcaggtgc 
      accggaagg ggggcgtgg ggaccccga cagtgccgg 
      acaaccggc ggaggccca tgtcgttcg tgcgggcct 
      tggagatgt ccgattcgg tcgagttga cagccccca 
      attcgcagt gttcgcact tgccccagc ggccctcca 
      cccccgaca ggaccagtg tctatgata tgaactatg 
      agatatcgg tatagggca ggtgttggc actcacgcg 
      ccgccgaat gagtgcatt tagtttctc tgcatcaga 
      gaccctcac tattaagta aacattccg tttactact 
      catccctgt gtcatggac ctaagacca accgtagtg 
      tgtgtaata taatctata tgttatttg ttgtttaca 
      atgtttaaa aagaattgt ttaattttg tgtatcaag 
      aaaccgtgg cttgtgttg ttgttttac cgttgattt 
      gacacgttt ttattgttt taacctgac tgtaactcc 
      actaaccga aagcagcgg ctcaggcat cttttctac 
      atgagttgt tgagtgtat taatggtgt aaatatatt 
      taagtttta gtagattcg tccttcaat caaacccga 
      atctcagcg aaagtttta ttaaacaca ctaaaccta 
      catttataa aaaaggatg taataaata aattaatga 
      atcaaagcc gtcttaaac tgttcttgt agcctcgac 
      atcgcctca cgagctcga acttcctgt atctcatgc 
      agtgccact gcgggatct gcgccataa gttcaatcg 
      cctgcaaaa ataacaata ataattaat aattacaaa 
      aacccaaac tgaaataca cgaaccgtt tcaggattg 
      ccaaccggc accgcccca cacccccga aagcaggcg 
      acaacgacc gccagcacg acaaacaac ttaacgagc 
      ttcatttta tgcaactga accgatgca ctgaacccc 
      cttatttat atgatgcaa aatagatca acctacttt 
      tcgatcgct tcaaatatt actcagtaa cctacttat 
      taaatcggt aatcacccg aaaattagg taattttcc 
      cgcagaact tgtcggcgc taccactga actaattca 
      attca
    • >TC002345 RC
      agcactgcc gtccgtttc aattcaagt aattaatgt 
      gaaaaatcg atataaaag tgtgcgact tggggaatt 
      tcttttccc ccgattttg tcaagtgtc agatgagtt 
      tgacagggg caacgaacg ataacttct cccgtttgt 
      ctcagccat gttggcctg tctgaggcg aaaattcga 
      gttttgcgc gagatcttg agtaaaaaa acattatgc 
      ccaagtgcg atgttaaaa ctagataca taccagcga 
      cttttgctt ggactttac tgttggtag ccacaacgt 
      tgttcttct actacccag cccgatact acttagagg 
      attatccat gggtgcccg cgtaccaag gagtgatca 
      cgttcttcg tcctcgcca atttcaccc tcgctacgt 
      tcatggacc cgggggtca taccaaaag cccctcctg 
      atgaagacc gcgaggacg atttcaggg cccccttgt 
      acaagaacg tggagatca acgggatta cggtgcgga 
      tgaaatgtg tttgtaacc actgtatag aaacttttg 
      accaccact gtccatggg tcaataatt gcattggga 
      gaagaaatt accgatttt tctttttct ttctcatct 
      cacttagct tacacatga tcagtatat ttactttaa 
      gcctaattt acatcctca aatatggtg atactttct 
      ccaacgctg aaccaatta ttgcttttg tcttaatgg 
      gtttggtgg ctttgttag ctataccaa tttttggct 
      taacagggt ttcatatgg ttttggtct cgagaggaa 
      ggaccacca atgaacaag tcacgggga aatttaagg 
      gcggttaca accccttct cgaggggct gctgggaca 
      actgctgct acacgcagt ttgggcccc aattcccaa 
      gtctcataa agccccaca agtacaacg tgaagcgca 
      agcactgcc cccatggcc ccatcgcga ccataacaa 
      acgacagtc aggtcaaga cctacatgg ataacagca 
      acggcatcc gcaacatca actccaacg cctacaaca 
      agttgtccc ctggtcgag acggctgtg aactggaca 
      tggagccgt cggcgtcac aatcgcaag attgcgagc 
      cgacgcccc ctcttcagc gacacggct ccaaaagca 
      acttttttc tgccgccga tggagggcg aatcgccga 
      gacaccccc gtggcgttc actacccgc gaagcagcc 
      cccatcctc ggccgcggg gcttagacc cgaatcgga 
      gcggaacgc cggagagtt tggggcccc aaaggccgt 
      cgccgacga tgcagcaga ggatcaagg cgttgggag 
      tgcccacgc ctttagcaa tgtctagtc ctgtgagga 
      gatccaacc cgagcaccc cgacccagc cacggcggc 
      cggatttca tcggcgtct ctgccaacc accagccgg 
      gcaactact atgacttcc aagcacatc ccaactcca 
      accagcgcc ctgccatgt ctcaggggg gctacggca 
      gccctcagc gccgtttca tgtccgagg gcgagctcg 
      tccgccagg aggcgcaac tgtcctacc cccgctcca 
      acaacacgg tggataaca tccgcgagt tggccaact 
      caccgcagc gcggcgtct acatgtgga aggacacct 
      cgccggggt atccgcccc cgcagcacc cggacttct 
      accgctcca accccacca gccccacgc agcaagcgt 
      acgccccca ggccctacc atccggccg tgcggggcg 
      gcgtcgccg tctacccac cacagagtc cgcaggagt 
      ttggtgggt tgcaggtgc accggaagg ggggcgtgg 
      ggaccccga cagtgccgg acaaccggc ggaggccca 
      tgtcgttcg tgcgggcct tggagatgt ccgattcgg 
      tcgagttga cagccccca attcgcagt gttcgcact 
      tgccccagc ggccctcca cccccgaca ggaccagtg 
      tctatgata tgaactatg agatatcgg tatagggca 
      ggtgttggc actcacgcg ccgccgaat gagtgcatt 
      tagtttctc tgcatcaga gaccctcac tattaagta 
      aacattccg tttactact catccctgt gtcatggac 
      ctaagacca accgtagtg tgtgtaata taatctata 
      tgttatttg ttgtttaca atgtttaaa aagaattgt 
      ttaattttg tgtatcaag aaaccgtgg cttgtgttg 
      ttgttttac cgttgattt gacacgttt ttattgttt 
      taacctgac tgtaactcc actaaccga aagcagcgg 
      ctcaggcat cttttctac atgagttgt tgagtgtat 
      taatggtgt aaatatatt taagtttta gtagattcg 
      tccttcaat caaacccg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY