| General information | |||
| TC002345 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452276.1: 101794..112371 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002345 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06102 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002345 RA
MPKCDVKTR YIPATFAWT LLLVATTLF FYYPARYYL EDYPWVPAY QGVITFFVL ANFTLATFM DPGVIPKAP PDEDREDDF RAPLYKNVE INGITVRMK CFVLMGLVA LLAIPIFGL TGFHMVLVS RGRTTNEQV TGKFKGGYN PFSRGCWDN CCYTQFGPQ FPSLIKPHK YNVKRKHCP HGPIATITN DSQVKTYMD NSNGIRNIN SNAYNKLSP GRDGCELDM EPSASQSQD CEPTPPLQR HGSKSNFFL PPMEGESPR HPRGVHYPR SSPHPRPRG LDPNRSGTP ESLGPQRPS PTMQQRIKA LGVPTPLAM SSPVRRSNP STPTQPRRP DFIGVSANH QPGNYYDFQ AHPNSNQRP AMSQGGYGS PQRRFMSEG ELVRQEAQL SYPRSNNTV DNIRELANS PQRGVYMWK DTSPGYPPP QHPDFYRSN PTSPTQQAY APRPYHPAV RGGVAVYPP QSPQEFGGL QVHRKGGVG TPTVPDNRR RPMSFVRAL EMSDSVELT APNSQCSHL PQRPSTPDR TSVYDMNYE ISV
- >TC002345 RB
MPKCDVKTR YIPATFAWT LLLVATTLF FYYPARYYL EDYPWVPAY QGVITFFVL ANFTLATFM DPGVIPKAP PDEDREDDF RAPLYKNVE INGITVRMK CVCNHCIET FDHHCPWVN NCIGRRNYR FFFFFLISL SLHMISIFT LSLIYILKY GDTFSNAEP IIAFVLMGL VALLAIPIF GLTGFHMVL VSRGRTTNE QVTGKFKGG YNPFSRGCW DNCCYTQFG PQFPSLIKP HKYNVKRKH CPHGPIATI TNDSQVKTY MDNSNGIRN INSNAYNKL SPGRDGCEL DMEPSASQS QDCEPTPPL QRHGSKSNF FLPPMEGES PRHPRGVHY PRSSPHPRP RGLDPNRSG TPESLGPQR PSPTMQQRI KALGVPTPL AMSSPVRRS NPSTPTQPR RPDFIGVSA NHQPGNYYD FQAHPNSNQ RPAMSQGGY GSPQRRFMS EGELVRQEA QLSYPRSNN TVDNIRELA NSPQRGVYM WKDTSPGYP PPQHPDFYR SNPTSPTQQ AYAPRPYHP AVRGGVAVY PPQSPQVHR KGGVGTPTV PDNRRRPMS FVRALEMSD SVELTAPNS QCSHLPQRP STPDRTSVY DMNYEISV
- >TC002345 RC
MPKCDVKTR YIPATFAWT LLLVATTLF FYYPARYYL EDYPWVPAY QGVITFFVL ANFTLATFM DPGVIPKAP PDEDREDDF RAPLYKNVE INGITVRMK CVCNHCIET FDHHCPWVN NCIGRRNYR FFFFFLISL SLHMISIFT LSLIYILKY GDTFSNAEP IIAFVLMGL VALLAIPIF GLTGFHMVL VSRGRTTNE QVTGKFKGG YNPFSRGCW DNCCYTQFG PQFPSLIKP HKYNVKRKH CPHGPIATI TNDSQVKTY MDNSNGIRN INSNAYNKL SPGRDGCEL DMEPSASQS QDCEPTPPL QRHGSKSNF FLPPMEGES PRHPRGVHY PRSSPHPRP RGLDPNRSG TPESLGPQR PSPTMQQRI KALGVPTPL AMSSPVRRS NPSTPTQPR RPDFIGVSA NHQPGNYYD FQAHPNSNQ RPAMSQGGY GSPQRRFMS EGELVRQEA QLSYPRSNN TVDNIRELA NSPQRGVYM WKDTSPGYP PPQHPDFYR SNPTSPTQQ AYAPRPYHP AVRGGVAVY PPQSPQEFG GLQVHRKGG VGTPTVPDN RRRPMSFVR ALEMSDSVE LTAPNSQCS HLPQRPSTP DRTSVYDMN YEISV
- >TC002345 RA
atgcccaag tgcgatgtt aaaactaga tacatacca gcgactttt gcttggact ttactgttg gtagccaca acgttgttc ttctactac ccagcccga tactactta gaggattat ccatgggtg cccgcgtac caaggagtg atcacgttc ttcgtcctc gccaatttc accctcgct acgttcatg gacccgggg gtcatacca aaagcccct cctgatgaa gaccgcgag gacgatttc agggccccc ttgtacaag aacgtggag atcaacggg attacggtg cggatgaaa tgttttgtc ttaatgggt ttggtggct ttgttagct ataccaatt tttggctta acagggttt catatggtt ttggtctcg agaggaagg accaccaat gaacaagtc acggggaaa tttaagggc ggttacaac cccttctcg aggggctgc tgggacaac tgctgctac acgcagttt gggccccaa ttcccaagt ctcataaag ccccacaag tacaacgtg aagcgcaag cactgcccc catggcccc atcgcgacc ataacaaac gacagtcag gtcaagacc tacatggat aacagcaac ggcatccgc aacatcaac tccaacgcc tacaacaag ttgtcccct ggtcgagac ggctgtgaa ctggacatg gagccgtcg gcgtcacaa tcgcaagat tgcgagccg acgccccct cttcagcga cacggctcc aaaagcaac ttttttctg ccgccgatg gagggcgaa tcgccgaga cacccccgt ggcgttcac tacccgcga agcagcccc catcctcgg ccgcggggc ttagacccg aatcggagc ggaacgccg gagagtttg gggccccaa aggccgtcg ccgacgatg cagcagagg atcaaggcg ttgggagtg cccacgcct ttagcaatg tctagtcct gtgaggaga tccaacccg agcaccccg acccagcca cggcggccg gatttcatc ggcgtctct gccaaccac cagccgggc aactactat gacttccaa gcacatccc aactccaac cagcgccct gccatgtct caggggggc tacggcagc cctcagcgc cgtttcatg tccgagggc gagctcgtc cgccaggag gcgcaactg tcctacccc cgctccaac aacacggtg gataacatc cgcgagttg gccaactca ccgcagcgc ggcgtctac atgtggaag gacacctcg ccggggtat ccgcccccg cagcacccg gacttctac cgctccaac cccaccagc cccacgcag caagcgtac gcccccagg ccctaccat ccggccgtg cggggcggc gtcgccgtc tacccacca cagagtccg caggagttt ggtgggttg caggtgcac cggaagggg ggcgtgggg accccgaca gtgccggac aaccggcgg aggcccatg tcgttcgtg cgggccttg gagatgtcc gattcggtc gagttgaca gcccccaat tcgcagtgt tcgcacttg ccccagcgg ccctccacc cccgacagg accagtgtc tatgatatg aactatgag atatcggta tag
- >TC002345 RB
atgcccaag tgcgatgtt aaaactaga tacatacca gcgactttt gcttggact ttactgttg gtagccaca acgttgttc ttctactac ccagcccga tactactta gaggattat ccatgggtg cccgcgtac caaggagtg atcacgttc ttcgtcctc gccaatttc accctcgct acgttcatg gacccgggg gtcatacca aaagcccct cctgatgaa gaccgcgag gacgatttc agggccccc ttgtacaag aacgtggag atcaacggg attacggtg cggatgaaa tgtgtttgt aaccactgt atagaaact tttgaccac cactgtcca tgggtcaat aattgcatt gggagaaga aattaccga tttttcttt ttctttctc atctcactt agcttacac atgatcagt atatttact ttaagccta atttacatc ctcaaatat ggtgatact ttctccaac gctgaacca attattgct tttgtctta atgggtttg gtggctttg ttagctata ccaattttt ggcttaaca gggtttcat atggttttg gtctcgaga ggaaggacc accaatgaa caagtcacg gggaaattt aagggcggt tacaacccc ttctcgagg ggctgctgg gacaactgc tgctacacg cagtttggg ccccaattc ccaagtctc ataaagccc cacaagtac aacgtgaag cgcaagcac tgcccccat ggccccatc gcgaccata acaaacgac agtcaggtc aagacctac atggataac agcaacggc atccgcaac atcaactcc aacgcctac aacaagttg tcccctggt cgagacggc tgtgaactg gacatggag ccgtcggcg tcacaatcg caagattgc gagccgacg ccccctctt cagcgacac ggctccaaa agcaacttt tttctgccg ccgatggag ggcgaatcg ccgagacac ccccgtggc gttcactac ccgcgaagc agcccccat cctcggccg cggggctta gacccgaat cggagcgga acgccggag agtttgggg ccccaaagg ccgtcgccg acgatgcag cagaggatc aaggcgttg ggagtgccc acgccttta gcaatgtct agtcctgtg aggagatcc aacccgagc accccgacc cagccacgg cggccggat ttcatcggc gtctctgcc aaccaccag ccgggcaac tactatgac ttccaagca catcccaac tccaaccag cgccctgcc atgtctcag gggggctac ggcagccct cagcgccgt ttcatgtcc gagggcgag ctcgtccgc caggaggcg caactgtcc tacccccgc tccaacaac acggtggat aacatccgc gagttggcc aactcaccg cagcgcggc gtctacatg tggaaggac acctcgccg gggtatccg cccccgcag cacccggac ttctaccgc tccaacccc accagcccc acgcagcaa gcgtacgcc cccaggccc taccatccg gccgtgcgg ggcggcgtc gccgtctac ccaccacag agtccgcag gtgcaccgg aaggggggc gtggggacc ccgacagtg ccggacaac cggcggagg cccatgtcg ttcgtgcgg gccttggag atgtccgat tcggtcgag ttgacagcc cccaattcg cagtgttcg cacttgccc cagcggccc tccaccccc gacaggacc agtgtctat gatatgaac tatgagata tcggtatag
- >TC002345 RC
atgcccaag tgcgatgtt aaaactaga tacatacca gcgactttt gcttggact ttactgttg gtagccaca acgttgttc ttctactac ccagcccga tactactta gaggattat ccatgggtg cccgcgtac caaggagtg atcacgttc ttcgtcctc gccaatttc accctcgct acgttcatg gacccgggg gtcatacca aaagcccct cctgatgaa gaccgcgag gacgatttc agggccccc ttgtacaag aacgtggag atcaacggg attacggtg cggatgaaa tgtgtttgt aaccactgt atagaaact tttgaccac cactgtcca tgggtcaat aattgcatt gggagaaga aattaccga tttttcttt ttctttctc atctcactt agcttacac atgatcagt atatttact ttaagccta atttacatc ctcaaatat ggtgatact ttctccaac gctgaacca attattgct tttgtctta atgggtttg gtggctttg ttagctata ccaattttt ggcttaaca gggtttcat atggttttg gtctcgaga ggaaggacc accaatgaa caagtcacg gggaaattt aagggcggt tacaacccc ttctcgagg ggctgctgg gacaactgc tgctacacg cagtttggg ccccaattc ccaagtctc ataaagccc cacaagtac aacgtgaag cgcaagcac tgcccccat ggccccatc gcgaccata acaaacgac agtcaggtc aagacctac atggataac agcaacggc atccgcaac atcaactcc aacgcctac aacaagttg tcccctggt cgagacggc tgtgaactg gacatggag ccgtcggcg tcacaatcg caagattgc gagccgacg ccccctctt cagcgacac ggctccaaa agcaacttt tttctgccg ccgatggag ggcgaatcg ccgagacac ccccgtggc gttcactac ccgcgaagc agcccccat cctcggccg cggggctta gacccgaat cggagcgga acgccggag agtttgggg ccccaaagg ccgtcgccg acgatgcag cagaggatc aaggcgttg ggagtgccc acgccttta gcaatgtct agtcctgtg aggagatcc aacccgagc accccgacc cagccacgg cggccggat ttcatcggc gtctctgcc aaccaccag ccgggcaac tactatgac ttccaagca catcccaac tccaaccag cgccctgcc atgtctcag gggggctac ggcagccct cagcgccgt ttcatgtcc gagggcgag ctcgtccgc caggaggcg caactgtcc tacccccgc tccaacaac acggtggat aacatccgc gagttggcc aactcaccg cagcgcggc gtctacatg tggaaggac acctcgccg gggtatccg cccccgcag cacccggac ttctaccgc tccaacccc accagcccc acgcagcaa gcgtacgcc cccaggccc taccatccg gccgtgcgg ggcggcgtc gccgtctac ccaccacag agtccgcag gagtttggt gggttgcag gtgcaccgg aaggggggc gtggggacc ccgacagtg ccggacaac cggcggagg cccatgtcg ttcgtgcgg gccttggag atgtccgat tcggtcgag ttgacagcc cccaattcg cagtgttcg cacttgccc cagcggccc tccaccccc gacaggacc agtgtctat gatatgaac tatgagata tcggtatag
- >TC002345 RA
agcactgcc gtccgtttc aattcaagt aattaatgt gaaaaatcg atataaaag tgtgcgact tggggaatt tcttttccc ccgattttg tcaagtgtc agatgagtt tgacagggg caacgaacg ataacttct cccgtttgt ctcagccat gttggcctg tctgaggcg aaaattcga gttttgcgc gagatcttg agtaaaaaa acattatgc ccaagtgcg atgttaaaa ctagataca taccagcga cttttgctt ggactttac tgttggtag ccacaacgt tgttcttct actacccag cccgatact acttagagg attatccat gggtgcccg cgtaccaag gagtgatca cgttcttcg tcctcgcca atttcaccc tcgctacgt tcatggacc cgggggtca taccaaaag cccctcctg atgaagacc gcgaggacg atttcaggg cccccttgt acaagaacg tggagatca acgggatta cggtgcgga tgaaatgtt ttgtcttaa tgggtttgg tggctttgt tagctatac caatttttg gcttaacag ggtttcata tggttttgg tctcgagag gaaggacca ccaatgaac aagtcacgg ggaaattta agggcggtt acaacccct tctcgaggg gctgctggg acaactgct gctacacgc agtttgggc cccaattcc caagtctca taaagcccc acaagtaca acgtgaagc gcaagcact gcccccatg gccccatcg cgaccataa caaacgaca gtcaggtca agacctaca tggataaca gcaacggca tccgcaaca tcaactcca acgcctaca acaagttgt cccctggtc gagacggct gtgaactgg acatggagc cgtcggcgt cacaatcgc aagattgcg agccgacgc cccctcttc agcgacacg gctccaaaa gcaactttt ttctgccgc cgatggagg gcgaatcgc cgagacacc cccgtggcg ttcactacc cgcgaagca gcccccatc ctcggccgc ggggcttag acccgaatc ggagcggaa cgccggaga gtttggggc cccaaaggc cgtcgccga cgatgcagc agaggatca aggcgttgg gagtgccca cgcctttag caatgtcta gtcctgtga ggagatcca acccgagca ccccgaccc agccacggc ggccggatt tcatcggcg tctctgcca accaccagc cgggcaact actatgact tccaagcac atcccaact ccaaccagc gccctgcca tgtctcagg ggggctacg gcagccctc agcgccgtt tcatgtccg agggcgagc tcgtccgcc aggaggcgc aactgtcct acccccgct ccaacaaca cggtggata acatccgcg agttggcca actcaccgc agcgcggcg tctacatgt ggaaggaca cctcgccgg ggtatccgc ccccgcagc acccggact tctaccgct ccaacccca ccagcccca cgcagcaag cgtacgccc ccaggccct accatccgg ccgtgcggg gcggcgtcg ccgtctacc caccacaga gtccgcagg agtttggtg ggttgcagg tgcaccgga aggggggcg tggggaccc cgacagtgc cggacaacc ggcggaggc ccatgtcgt tcgtgcggg ccttggaga tgtccgatt cggtcgagt tgacagccc ccaattcgc agtgttcgc acttgcccc agcggccct ccacccccg acaggacca gtgtctatg atatgaact atgagatat cggtatagg gcaggtgtt ggcactcac gcgccgccg aatgagtgc atttagttt ctctgcatc agagaccct cactattaa gtaaacatt ccgtttact actcatccc tgtgtcatg gacctaaga ccaaccgta gtgtgtgta atataatct atatgttat ttgttgttt acaatgttt aaaaagaat tgtttaatt ttgtgtatc aagaaaccg tggcttgtg ttgttgttt taccgttga tttgacacg tttttattg ttttaacct gactgtaac tccactaac cgaaagcag cggctcagg catcttttc tacatgagt tgttgagtg tattaatgg tgtaaatat atttaagtt ttagtagat tcgtccttc aatcaaacc cgaatctca gcgaaagtt ttattaaac acactaaac ctacattta taaaaaagg atgtaataa ataaattaa tgaatcaaa gccgtctta aactgttct tgtagcctc gacatcgcc tcacgagct cgaacttcc tgtatctca tgcagtgcc actgcggga tctgcgcca taagttcaa tcgcctgca aaaataaca ataataatt aataattac aaaaaccca aactgaaat acacgaacc gtttcagga ttgccaacc ggcaccgcc ccacacccc cgaaagcag gcgacaacg accgccagc acgacaaac aacttaacg agcttcatt ttatgcaac tgaaccgat gcactgaac ccccttatt tatatgatg caaaataga tcaacctac ttttcgatc gcttcaaat attactcag taacctact tattaaatc ggtaatcac ccgaaaatt aggtaattt tcccgcaga acttgtcgg cgctaccac tgaactaat tcaattca
- >TC002345 RB
agcactgcc gtccgtttc aattcaagt aattaatgt gaaaaatcg atataaaag tgtgcgact tggggaatt tcttttccc ccgattttg tcaagtgtc agatgagtt tgacagggg caacgaacg ataacttct cccgtttgt ctcagccat gttggcctg tctgaggcg aaaattcga gttttgcgc gagatcttg agtaaaaaa acattatgc ccaagtgcg atgttaaaa ctagataca taccagcga cttttgctt ggactttac tgttggtag ccacaacgt tgttcttct actacccag cccgatact acttagagg attatccat gggtgcccg cgtaccaag gagtgatca cgttcttcg tcctcgcca atttcaccc tcgctacgt tcatggacc cgggggtca taccaaaag cccctcctg atgaagacc gcgaggacg atttcaggg cccccttgt acaagaacg tggagatca acgggatta cggtgcgga tgaaatgtg tttgtaacc actgtatag aaacttttg accaccact gtccatggg tcaataatt gcattggga gaagaaatt accgatttt tctttttct ttctcatct cacttagct tacacatga tcagtatat ttactttaa gcctaattt acatcctca aatatggtg atactttct ccaacgctg aaccaatta ttgcttttg tcttaatgg gtttggtgg ctttgttag ctataccaa tttttggct taacagggt ttcatatgg ttttggtct cgagaggaa ggaccacca atgaacaag tcacgggga aatttaagg gcggttaca accccttct cgaggggct gctgggaca actgctgct acacgcagt ttgggcccc aattcccaa gtctcataa agccccaca agtacaacg tgaagcgca agcactgcc cccatggcc ccatcgcga ccataacaa acgacagtc aggtcaaga cctacatgg ataacagca acggcatcc gcaacatca actccaacg cctacaaca agttgtccc ctggtcgag acggctgtg aactggaca tggagccgt cggcgtcac aatcgcaag attgcgagc cgacgcccc ctcttcagc gacacggct ccaaaagca acttttttc tgccgccga tggagggcg aatcgccga gacaccccc gtggcgttc actacccgc gaagcagcc cccatcctc ggccgcggg gcttagacc cgaatcgga gcggaacgc cggagagtt tggggcccc aaaggccgt cgccgacga tgcagcaga ggatcaagg cgttgggag tgcccacgc ctttagcaa tgtctagtc ctgtgagga gatccaacc cgagcaccc cgacccagc cacggcggc cggatttca tcggcgtct ctgccaacc accagccgg gcaactact atgacttcc aagcacatc ccaactcca accagcgcc ctgccatgt ctcaggggg gctacggca gccctcagc gccgtttca tgtccgagg gcgagctcg tccgccagg aggcgcaac tgtcctacc cccgctcca acaacacgg tggataaca tccgcgagt tggccaact caccgcagc gcggcgtct acatgtgga aggacacct cgccggggt atccgcccc cgcagcacc cggacttct accgctcca accccacca gccccacgc agcaagcgt acgccccca ggccctacc atccggccg tgcggggcg gcgtcgccg tctacccac cacagagtc cgcaggtgc accggaagg ggggcgtgg ggaccccga cagtgccgg acaaccggc ggaggccca tgtcgttcg tgcgggcct tggagatgt ccgattcgg tcgagttga cagccccca attcgcagt gttcgcact tgccccagc ggccctcca cccccgaca ggaccagtg tctatgata tgaactatg agatatcgg tatagggca ggtgttggc actcacgcg ccgccgaat gagtgcatt tagtttctc tgcatcaga gaccctcac tattaagta aacattccg tttactact catccctgt gtcatggac ctaagacca accgtagtg tgtgtaata taatctata tgttatttg ttgtttaca atgtttaaa aagaattgt ttaattttg tgtatcaag aaaccgtgg cttgtgttg ttgttttac cgttgattt gacacgttt ttattgttt taacctgac tgtaactcc actaaccga aagcagcgg ctcaggcat cttttctac atgagttgt tgagtgtat taatggtgt aaatatatt taagtttta gtagattcg tccttcaat caaacccga atctcagcg aaagtttta ttaaacaca ctaaaccta catttataa aaaaggatg taataaata aattaatga atcaaagcc gtcttaaac tgttcttgt agcctcgac atcgcctca cgagctcga acttcctgt atctcatgc agtgccact gcgggatct gcgccataa gttcaatcg cctgcaaaa ataacaata ataattaat aattacaaa aacccaaac tgaaataca cgaaccgtt tcaggattg ccaaccggc accgcccca cacccccga aagcaggcg acaacgacc gccagcacg acaaacaac ttaacgagc ttcatttta tgcaactga accgatgca ctgaacccc cttatttat atgatgcaa aatagatca acctacttt tcgatcgct tcaaatatt actcagtaa cctacttat taaatcggt aatcacccg aaaattagg taattttcc cgcagaact tgtcggcgc taccactga actaattca attca
- >TC002345 RC
agcactgcc gtccgtttc aattcaagt aattaatgt gaaaaatcg atataaaag tgtgcgact tggggaatt tcttttccc ccgattttg tcaagtgtc agatgagtt tgacagggg caacgaacg ataacttct cccgtttgt ctcagccat gttggcctg tctgaggcg aaaattcga gttttgcgc gagatcttg agtaaaaaa acattatgc ccaagtgcg atgttaaaa ctagataca taccagcga cttttgctt ggactttac tgttggtag ccacaacgt tgttcttct actacccag cccgatact acttagagg attatccat gggtgcccg cgtaccaag gagtgatca cgttcttcg tcctcgcca atttcaccc tcgctacgt tcatggacc cgggggtca taccaaaag cccctcctg atgaagacc gcgaggacg atttcaggg cccccttgt acaagaacg tggagatca acgggatta cggtgcgga tgaaatgtg tttgtaacc actgtatag aaacttttg accaccact gtccatggg tcaataatt gcattggga gaagaaatt accgatttt tctttttct ttctcatct cacttagct tacacatga tcagtatat ttactttaa gcctaattt acatcctca aatatggtg atactttct ccaacgctg aaccaatta ttgcttttg tcttaatgg gtttggtgg ctttgttag ctataccaa tttttggct taacagggt ttcatatgg ttttggtct cgagaggaa ggaccacca atgaacaag tcacgggga aatttaagg gcggttaca accccttct cgaggggct gctgggaca actgctgct acacgcagt ttgggcccc aattcccaa gtctcataa agccccaca agtacaacg tgaagcgca agcactgcc cccatggcc ccatcgcga ccataacaa acgacagtc aggtcaaga cctacatgg ataacagca acggcatcc gcaacatca actccaacg cctacaaca agttgtccc ctggtcgag acggctgtg aactggaca tggagccgt cggcgtcac aatcgcaag attgcgagc cgacgcccc ctcttcagc gacacggct ccaaaagca acttttttc tgccgccga tggagggcg aatcgccga gacaccccc gtggcgttc actacccgc gaagcagcc cccatcctc ggccgcggg gcttagacc cgaatcgga gcggaacgc cggagagtt tggggcccc aaaggccgt cgccgacga tgcagcaga ggatcaagg cgttgggag tgcccacgc ctttagcaa tgtctagtc ctgtgagga gatccaacc cgagcaccc cgacccagc cacggcggc cggatttca tcggcgtct ctgccaacc accagccgg gcaactact atgacttcc aagcacatc ccaactcca accagcgcc ctgccatgt ctcaggggg gctacggca gccctcagc gccgtttca tgtccgagg gcgagctcg tccgccagg aggcgcaac tgtcctacc cccgctcca acaacacgg tggataaca tccgcgagt tggccaact caccgcagc gcggcgtct acatgtgga aggacacct cgccggggt atccgcccc cgcagcacc cggacttct accgctcca accccacca gccccacgc agcaagcgt acgccccca ggccctacc atccggccg tgcggggcg gcgtcgccg tctacccac cacagagtc cgcaggagt ttggtgggt tgcaggtgc accggaagg ggggcgtgg ggaccccga cagtgccgg acaaccggc ggaggccca tgtcgttcg tgcgggcct tggagatgt ccgattcgg tcgagttga cagccccca attcgcagt gttcgcact tgccccagc ggccctcca cccccgaca ggaccagtg tctatgata tgaactatg agatatcgg tatagggca ggtgttggc actcacgcg ccgccgaat gagtgcatt tagtttctc tgcatcaga gaccctcac tattaagta aacattccg tttactact catccctgt gtcatggac ctaagacca accgtagtg tgtgtaata taatctata tgttatttg ttgtttaca atgtttaaa aagaattgt ttaattttg tgtatcaag aaaccgtgg cttgtgttg ttgttttac cgttgattt gacacgttt ttattgttt taacctgac tgtaactcc actaaccga aagcagcgg ctcaggcat cttttctac atgagttgt tgagtgtat taatggtgt aaatatatt taagtttta gtagattcg tccttcaat caaacccg
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