Status
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Gene information
General information
TC002351 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 25061..30003
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002351
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06101

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002351 RA
      MEIDEDSLC EDSGNETSG DDVDFNVEV ESTAKDPQP 
      YDDEYQYEV LSTSDIVQY MSDIIDEVN NVVQLPPTT 
      TRILLNYFN WDKEKLMER FFDGDQDEL FKEANVINP 
      FKSLPHATR PKFSLKIKG TEECDICFM VCAPAHMTG 
      LECGHRFCY QCWNEYLTT KVVEEGVGQ TIACPALNC 
      PILVDDETV MRLVKDSRV KIKYQHLIT NSFIECNRL 
      LRWCPSPDC NYAVKVSYV DAKPVTCIC THVFCFSCG 
      ENWHDPVKC SLLKKWIKK CDDDSETSN WISANTKEC 
      PKCGATIEK DGGCNHMVC KNQNCKADF CWVCLGSWE 
      PHGSSWYNC NKYDEDEAK AARDAQERS RAALQRYLF 
      YCNRYMNHM QSLKFEHKL YSSVKDKME EMQQHNMSW 
      IEVQFLKKA VDILCKCRQ TLMYTYVFA YYLKKNNQS 
      VIFEDNQKD LERATELLS EYLERDITQ ENLVDIKQK 
      VQDKYRYCD GRRKALLDH VHEGYEKDW WVCKEA
    CDS sequence
    • >TC002351 RA
      atggagatc gacgaagac agcttgtgc gaagactcg 
      ggtaacgag accagcggc gacgacgtc gatttcaac 
      gtggaggtg gagagcacc gccaaggac ccgcagccc 
      tacgacgac gagtaccag tacgaggtg ctctccacc 
      agcgacatc gtccagtac atgtcggac atcatcgac 
      gaggtgaac aacgtggtg cagctgccg ccgacgacg 
      acgcgcatc ctcctcaac tacttcaac tgggacaag 
      gagaagctg atggagcgc ttcttcgac ggggaccaa 
      gacgagctg ttcaaggag gccaacgtg ataaacccc 
      ttcaagagc ttaccgcac gccacgcgc cccaagttc 
      tcgctgaag atcaaaggg acggaggag tgcgacatt 
      tgcttcatg gtgtgcgcc cccgcgcac atgacgggc 
      ttggagtgc gggcaccgg ttttgctac cagtgttgg 
      aacgagtac ctcaccacg aaagttgta gaggagggc 
      gtggggcaa accattgct tgtcccgcc ctcaattgc 
      cccattctg gtcgacgat gagactgtt atgagacta 
      gtcaaggat tccagggtt aaaattaaa taccaacat 
      ttgattact aatagtttc atcgaatgc aacaggctg 
      ctacggtgg tgtcccagt cccgactgc aattacgcc 
      gtcaaggtg tcttacgtg gatgcgaaa ccagtgacg 
      tgtatctgc acccacgtg ttctgtttt tcatgcggt 
      gagaactgg cacgaccct gtcaagtgc agtttgctg 
      aagaaatgg atcaaaaag tgcgacgat gattccgaa 
      acgtctaac tggatctca gcaaatacg aaagagtgc 
      cccaaatgc ggggctact atcgagaaa gacggcggt 
      tgtaaccac atggtgtgc aaaaaccag aactgcaag 
      gccgatttc tgttgggtt tgtttgggt tcgtgggag 
      cctcacggc agttcgtgg tacaattgt aataagtat 
      gatgaagat gaggcgaag gcggcgcgc gatgcccag 
      gaaaggtca cgtgcggcc ctgcagcgc tatctgttt 
      tactgtaac cgatacatg aaccatatg cagtcgttg 
      aagttcgag cacaagctt tattcgtcg gttaaggat 
      aagatggag gagatgcag cagcacaat atgagctgg 
      attgaggtg caattcttg aagaaggcg gtggatatt 
      ttgtgtaaa tgcaggcag acgttgatg tatacgtat 
      gtctttgcg tattatttg aaaaagaat aatcagtct 
      gtgatattt gaagacaac caaaaagat ttagaacgt 
      gccacggag ctgctgagt gagtatctc gagagggat 
      ataactcag gagaatctt gttgatatt aaacaaaaa 
      gtacaggat aagtatagg tattgtgac gggaggagg 
      aaagctctg ttggatcac gtgcatgaa gggtatgaa 
      aaagattgg tgggtctgc aaggaggcg tag
    mRNA sequence
    • >TC002351 RA
      tgctacgta ttgatcggt ttaataagt gattttatt 
      taagtctgg gtttttatt gcgcggtat ttgtgccga 
      atgttttcg cccgtccct caccttttg ttacgtcgt 
      tggttggtg tttttgttc tctacgaaa ttatttccc 
      tcaccgttc aagaaatac aatttttgc taagtgtta 
      gtcgcagtt gagtgaatt atgtttagg ttatcatta 
      gtacagtaa attgtgtgt ttgttggta ttttgtcgt 
      cgtccaccg ccccaaaat gcccccatg cgccccttt 
      ctcgtagtt aatgtttgt ggtgacctg ctgtcaacg 
      agggcactc aatttcggc acgttgtca aaatcagac 
      agtgacaca acagcacaa cacttccag gagtcaaaa 
      aatggagat cgacgaaga cagcttgtg cgaagactc 
      gggtaacga gaccagcgg cgacgacgt cgatttcaa 
      cgtggaggt ggagagcac cgccaagga cccgcagcc 
      ctacgacga cgagtacca gtacgaggt gctctccac 
      cagcgacat cgtccagta catgtcgga catcatcga 
      cgaggtgaa caacgtggt gcagctgcc gccgacgac 
      gacgcgcat cctcctcaa ctacttcaa ctgggacaa 
      ggagaagct gatggagcg cttcttcga cggggacca 
      agacgagct gttcaagga ggccaacgt gataaaccc 
      cttcaagag cttaccgca cgccacgcg ccccaagtt 
      ctcgctgaa gatcaaagg gacggagga gtgcgacat 
      ttgcttcat ggtgtgcgc ccccgcgca catgacggg 
      cttggagtg cgggcaccg gttttgcta ccagtgttg 
      gaacgagta cctcaccac gaaagttgt agaggaggg 
      cgtggggca aaccattgc ttgtcccgc cctcaattg 
      ccccattct ggtcgacga tgagactgt tatgagact 
      agtcaagga ttccagggt taaaattaa ataccaaca 
      tttgattac taatagttt catcgaatg caacaggct 
      gctacggtg gtgtcccag tcccgactg caattacgc 
      cgtcaaggt gtcttacgt ggatgcgaa accagtgac 
      gtgtatctg cacccacgt gttctgttt ttcatgcgg 
      tgagaactg gcacgaccc tgtcaagtg cagtttgct 
      gaagaaatg gatcaaaaa gtgcgacga tgattccga 
      aacgtctaa ctggatctc agcaaatac gaaagagtg 
      ccccaaatg cggggctac tatcgagaa agacggcgg 
      ttgtaacca catggtgtg caaaaacca gaactgcaa 
      ggccgattt ctgttgggt ttgtttggg ttcgtggga 
      gcctcacgg cagttcgtg gtacaattg taataagta 
      tgatgaaga tgaggcgaa ggcggcgcg cgatgccca 
      ggaaaggtc acgtgcggc cctgcagcg ctatctgtt 
      ttactgtaa ccgatacat gaaccatat gcagtcgtt 
      gaagttcga gcacaagct ttattcgtc ggttaagga 
      taagatgga ggagatgca gcagcacaa tatgagctg 
      gattgaggt gcaattctt gaagaaggc ggtggatat 
      tttgtgtaa atgcaggca gacgttgat gtatacgta 
      tgtctttgc gtattattt gaaaaagaa taatcagtc 
      tgtgatatt tgaagacaa ccaaaaaga tttagaacg 
      tgccacgga gctgctgag tgagtatct cgagaggga 
      tataactca ggagaatct tgttgatat taaacaaaa 
      agtacagga taagtatag gtattgtga cgggaggag 
      gaaagctct gttggatca cgtgcatga agggtatga 
      aaaagattg gtgggtctg caaggaggc gtaggataa 
      cgttgggaa ttattggga tttttaaac aataaagtt 
      ttatttctt aaaagagtt tctcactaa ccggtgata 
      tgtatgcac ttttgggaa atattcatt gttccgagc 
      gaccaactg aactgaaga gtgcggaaa tgctatttt 
      tgtaatcat tctccaaac gctgaaata cattttatg 
      ccacaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY