Status
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Gene information
General information
TC032986
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002239 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 656356..666013
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032986
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    elytra surface irregular - Number of animals showing the phenotype: 9


    hindwing necrotic - Number of animals showing the phenotype: 5


    elytra dorsal not closed - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032986 RA
      MLVNNRRGR FIVLGSSGE CLPVTRKPV DSPPKSVYS 
SCESSEEEF LSAKTSLDD GSEDENFHK RYSIDLETP 
ERRHTFAQR LRDALRREV SDTFSSSSE SSYAVGISV 
AGSDVQDKQ IKVRRGGST GFALKEDSL DENFLQDSL 
QQEKQWIDK FKNKQSVLS RKESNKSSE YSFSTEYSS 
ELEEVYEQF SRWLQNPIL ETEKGTKKE LEGRDLAVV 
QFAGSLLKR TLSESFAGV PVPLAESAD ATRNLDFNP 
VKKNKLVLS AKSLSLELA RQKHKLAAQ LISQLTQRS 
ENGLKPVAT GNWGCGSSK CGDVQLKVI IQWLAASVA 
GIPCLKYYT CGHQKLTKL DTVCRILVD RKWTVKDLA 
QATLRYANQ VLHGREVSG TLFEELIGI ERTV
    • >TC032986 RB
      MLVNNRRGR FIVLGSSGE CLPVTRKPV DSPPKSVYS 
SCESSEEEF LSAKTSLDD GSEDENFHK RYSIDLETP 
ERRHTFAQR LRDALRREV SDTFSSSSE SSYAVGISV 
AGSDVQDKQ IKVRRGGST GFALKEDSL DENFLQDSL 
QQEKQWIDK FKNKQSVLS RKESNKSSE YSFSTEYSS 
ELEEVYEQF SRWLQNPIL ETEKGTKKE LEGRDLAVV 
QFAGSLLKR TLSESFAGV PVPLAESAD ATRNLDFNP 
VKKNKLVLS AKSLSLELA RQKHKLAAQ LMSKEPLTK 
EPVLSECNL TSKKRRAWF ISCVNEAIV QTLEEISCS 
VSLPKEKQI SQLTQRSEN GLKPVATGN WGCGSSKCG 
DVQLKVIIQ WLAASVAGI PCLKYYTCG HQKLTKLDT 
VCRILVDRK WTVKDLAQA TLRYANQVL HGREVSGTL 
FEELIGIER TV
    CDS sequence
    • >TC032986 RA
      atgctagtg aacaaccga cggggtagg ttcattgtg 
ttggggtcc tccggggaa tgtcttcca gtgacgaga 
aaaccggtg gattcgcct ccgaaaagt gtttattcc 
agctgtgaa tccagcgaa gaggagttt cttagtgcc 
aagacgagt ctagacgat ggaagcgaa gacgaaaat 
tttcacaag aggtacagt atcgatctg gagactccc 
gagaggcgt cacactttt gcgcagaga ctcagagat 
gcgttaagg agagaggtt tcagacact ttcagcagc 
tcatctgag tcgagttat gctgtgggg atttcagtg 
gccggctca gacgtgcaa gataagcaa attaaggtc 
cgacgtgga ggttccacc gggtttgcg ctcaaggag 
gactccctg gatgaaaat ttccttcaa gactcgttg 
caacaagaa aagcaatgg atcgacaaa ttcaaaaac 
aaacaatcc gttctgagc cgaaaagag tcaaacaag 
tcgtccgaa tacagtttt agtaccgaa tacagttcc 
gaattggaa gaagtttac gaacagttc tcgcgttgg 
ctccaaaac ccgatcttg gaaacggaa aaaggaact 
aagaaggag ttggaaggt cgcgatctg gctgtggtg 
caattcgct ggatcgtta ttaaaacgt actctcagt 
gaatccttc gctggggtt ccagtacca cttgcagaa 
agcgctgat gcgacacga aacctcgat ttcaaccca 
gttaagaaa aataagcta gtgctcagt gcaaaatcg 
ctcagtttg gagctggcg cgacaaaaa cacaaacta 
gcggctcaa ttaatttct cagctaacg cagaggtcg 
gaaaatggg ctaaaacca gtcgcgaca ggaaattgg 
ggttgtggc tcctccaag tgtggagat gtgcaacta 
aaagtgata atccaatgg ttggcggca agtgtggcg 
ggaatcccc tgccttaaa tactacact tgtggtcat 
cagaaattg acaaaattg gatacagtg tgccgaata 
ctcgttgat aggaaatgg actgttaag gatcttgct 
caagctaca cttaggtac gcaaatcaa gtgctccac 
ggacgtgaa gtcagtgga acacttttt gaggagtta 
atcggtata gaacggacg gtttga
    • >TC032986 RB
      atgctagtg aacaaccga cggggtagg ttcattgtg 
ttggggtcc tccggggaa tgtcttcca gtgacgaga 
aaaccggtg gattcgcct ccgaaaagt gtttattcc 
agctgtgaa tccagcgaa gaggagttt cttagtgcc 
aagacgagt ctagacgat ggaagcgaa gacgaaaat 
tttcacaag aggtacagt atcgatctg gagactccc 
gagaggcgt cacactttt gcgcagaga ctcagagat 
gcgttaagg agagaggtt tcagacact ttcagcagc 
tcatctgag tcgagttat gctgtgggg atttcagtg 
gccggctca gacgtgcaa gataagcaa attaaggtc 
cgacgtgga ggttccacc gggtttgcg ctcaaggag 
gactccctg gatgaaaat ttccttcaa gactcgttg 
caacaagaa aagcaatgg atcgacaaa ttcaaaaac 
aaacaatcc gttctgagc cgaaaagag tcaaacaag 
tcgtccgaa tacagtttt agtaccgaa tacagttcc 
gaattggaa gaagtttac gaacagttc tcgcgttgg 
ctccaaaac ccgatcttg gaaacggaa aaaggaact 
aagaaggag ttggaaggt cgcgatctg gctgtggtg 
caattcgct ggatcgtta ttaaaacgt actctcagt 
gaatccttc gctggggtt ccagtacca cttgcagaa 
agcgctgat gcgacacga aacctcgat ttcaaccca 
gttaagaaa aataagcta gtgctcagt gcaaaatcg 
ctcagtttg gagctggcg cgacaaaaa cacaaacta 
gcggctcaa ttaatgagt aaagagccc ttaacaaaa 
gaaccggtt ttgagtgaa tgtaattta acaagtaaa 
aagcgtaga gcttggttt atatcttgc gtaaatgaa 
gccatcgtc caaacttta gaagagatt tcctgtagc 
gttagttta cccaaagaa aaacagatt tctcagcta 
acgcagagg tcggaaaat gggctaaaa ccagtcgcg 
acaggaaat tggggttgt ggctcctcc aagtgtgga 
gatgtgcaa ctaaaagtg ataatccaa tggttggcg 
gcaagtgtg gcgggaatc ccctgcctt aaatactac 
acttgtggt catcagaaa ttgacaaaa ttggataca 
gtgtgccga atactcgtt gataggaaa tggactgtt 
aaggatctt gctcaagct acacttagg tacgcaaat 
caagtgctc cacggacgt gaagtcagt ggaacactt 
tttgaggag ttaatcggt atagaacgg acggtttga
    mRNA sequence
    • >TC032986 RA
      ttaattaaa cactgatca ttcatcatt ttataccga 
tcaaaatcg tattgatta ctgattgct ttattacaa 
ttctttcca aaaccagct tcgagtcca gctcgaagt 
cgagcgaaa tttgcagaa aaagggaca aattaacaa 
cgaaatgct agtgaacaa ccgacgggg taggttcat 
tgtgttggg gtcctccgg ggaatgtct tccagtgac 
gagaaaacc ggtggattc gcctccgaa aagtgttta 
ttccagctg tgaatccag cgaagagga gtttcttag 
tgccaagac gagtctaga cgatggaag cgaagacga 
aaattttca caagaggta cagtatcga tctggagac 
tcccgagag gcgtcacac ttttgcgca gagactcag 
agatgcgtt aaggagaga ggtttcaga cactttcag 
cagctcatc tgagtcgag ttatgctgt ggggatttc 
agtggccgg ctcagacgt gcaagataa gcaaattaa 
ggtccgacg tggaggttc caccgggtt tgcgctcaa 
ggaggactc cctggatga aaatttcct tcaagactc 
gttgcaaca agaaaagca atggatcga caaattcaa 
aaacaaaca atccgttct gagccgaaa agagtcaaa 
caagtcgtc cgaatacag ttttagtac cgaatacag 
ttccgaatt ggaagaagt ttacgaaca gttctcgcg 
ttggctcca aaacccgat cttggaaac ggaaaaagg 
aactaagaa ggagttgga aggtcgcga tctggctgt 
ggtgcaatt cgctggatc gttattaaa acgtactct 
cagtgaatc cttcgctgg ggttccagt accacttgc 
agaaagcgc tgatgcgac acgaaacct cgatttcaa 
cccagttaa gaaaaataa gctagtgct cagtgcaaa 
atcgctcag tttggagct ggcgcgaca aaaacacaa 
actagcggc tcaattaat ttctcagct aacgcagag 
gtcggaaaa tgggctaaa accagtcgc gacaggaaa 
ttggggttg tggctcctc caagtgtgg agatgtgca 
actaaaagt gataatcca atggttggc ggcaagtgt 
ggcgggaat cccctgcct taaatacta cacttgtgg 
tcatcagaa attgacaaa attggatac agtgtgccg 
aatactcgt tgataggaa atggactgt taaggatct 
tgctcaagc tacacttag gtacgcaaa tcaagtgct 
ccacggacg tgaagtcag tggaacact ttttgagga 
gttaatcgg tatagaacg gacggtttg aggatgtct 
agtcacact tcagttatt agcaaacac attcttata 
actttttta ctttagata aagtgagct tccgcttgt 
ttcaatgta cttgttttg ataaaaatg tgtaaggct 
gttctgctc aaaccccac attagaata ttgtgagtt 
gtaataacg aaaatatat gaaaatttg tgta
    • >TC032986 RB
      ttaattaaa cactgatca ttcatcatt ttataccga 
tcaaaatcg tattgatta ctgattgct ttattacaa 
ttctttcca aaaccagct tcgagtcca gctcgaagt 
cgagcgaaa tttgcagaa aaagggaca aattaacaa 
cgaaatgct agtgaacaa ccgacgggg taggttcat 
tgtgttggg gtcctccgg ggaatgtct tccagtgac 
gagaaaacc ggtggattc gcctccgaa aagtgttta 
ttccagctg tgaatccag cgaagagga gtttcttag 
tgccaagac gagtctaga cgatggaag cgaagacga 
aaattttca caagaggta cagtatcga tctggagac 
tcccgagag gcgtcacac ttttgcgca gagactcag 
agatgcgtt aaggagaga ggtttcaga cactttcag 
cagctcatc tgagtcgag ttatgctgt ggggatttc 
agtggccgg ctcagacgt gcaagataa gcaaattaa 
ggtccgacg tggaggttc caccgggtt tgcgctcaa 
ggaggactc cctggatga aaatttcct tcaagactc 
gttgcaaca agaaaagca atggatcga caaattcaa 
aaacaaaca atccgttct gagccgaaa agagtcaaa 
caagtcgtc cgaatacag ttttagtac cgaatacag 
ttccgaatt ggaagaagt ttacgaaca gttctcgcg 
ttggctcca aaacccgat cttggaaac ggaaaaagg 
aactaagaa ggagttgga aggtcgcga tctggctgt 
ggtgcaatt cgctggatc gttattaaa acgtactct 
cagtgaatc cttcgctgg ggttccagt accacttgc 
agaaagcgc tgatgcgac acgaaacct cgatttcaa 
cccagttaa gaaaaataa gctagtgct cagtgcaaa 
atcgctcag tttggagct ggcgcgaca aaaacacaa 
actagcggc tcaattaat gagtaaaga gcccttaac 
aaaagaacc ggttttgag tgaatgtaa tttaacaag 
taaaaagcg tagagcttg gtttatatc ttgcgtaaa 
tgaagccat cgtccaaac tttagaaga gatttcctg 
tagcgttag tttacccaa agaaaaaca gatttctca 
gctaacgca gaggtcgga aaatgggct aaaaccagt 
cgcgacagg aaattgggg ttgtggctc ctccaagtg 
tggagatgt gcaactaaa agtgataat ccaatggtt 
ggcggcaag tgtggcggg aatcccctg ccttaaata 
ctacacttg tggtcatca gaaattgac aaaattgga 
tacagtgtg ccgaatact cgttgatag gaaatggac 
tgttaagga tcttgctca agctacact taggtacgc 
aaatcaagt gctccacgg acgtgaagt cagtggaac 
actttttga ggagttaat cggtataga acggacggt 
ttgaggatg tctagtcac acttcagtt attagcaaa 
cacattctt ataactttt ttactttag ataaagtga 
gcttccgct tgtttcaat gtacttgtt ttgataaaa 
atgtgtaag gctgttctg ctcaaaccc cacattaga 
atattgtga gttgtaata acgaaaata tatgaaaat 
ttgtgta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY