Status
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Gene information
General information
TC032986
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002239
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 656356..666013
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032986
    iBeetle screen iB_06100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    elytra surface irregular - Number of animals showing the phenotype: 9


    hindwing necrotic - Number of animals showing the phenotype: 5


    elytra dorsal not closed - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032986 RA
      MLVNNRRGR FIVLGSSGE CLPVTRKPV DSPPKSVYS 
      SCESSEEEF LSAKTSLDD GSEDENFHK RYSIDLETP 
      ERRHTFAQR LRDALRREV SDTFSSSSE SSYAVGISV 
      AGSDVQDKQ IKVRRGGST GFALKEDSL DENFLQDSL 
      QQEKQWIDK FKNKQSVLS RKESNKSSE YSFSTEYSS 
      ELEEVYEQF SRWLQNPIL ETEKGTKKE LEGRDLAVV 
      QFAGSLLKR TLSESFAGV PVPLAESAD ATRNLDFNP 
      VKKNKLVLS AKSLSLELA RQKHKLAAQ LISQLTQRS 
      ENGLKPVAT GNWGCGSSK CGDVQLKVI IQWLAASVA 
      GIPCLKYYT CGHQKLTKL DTVCRILVD RKWTVKDLA 
      QATLRYANQ VLHGREVSG TLFEELIGI ERTV
    • >TC032986 RB
      MLVNNRRGR FIVLGSSGE CLPVTRKPV DSPPKSVYS 
      SCESSEEEF LSAKTSLDD GSEDENFHK RYSIDLETP 
      ERRHTFAQR LRDALRREV SDTFSSSSE SSYAVGISV 
      AGSDVQDKQ IKVRRGGST GFALKEDSL DENFLQDSL 
      QQEKQWIDK FKNKQSVLS RKESNKSSE YSFSTEYSS 
      ELEEVYEQF SRWLQNPIL ETEKGTKKE LEGRDLAVV 
      QFAGSLLKR TLSESFAGV PVPLAESAD ATRNLDFNP 
      VKKNKLVLS AKSLSLELA RQKHKLAAQ LMSKEPLTK 
      EPVLSECNL TSKKRRAWF ISCVNEAIV QTLEEISCS 
      VSLPKEKQI SQLTQRSEN GLKPVATGN WGCGSSKCG 
      DVQLKVIIQ WLAASVAGI PCLKYYTCG HQKLTKLDT 
      VCRILVDRK WTVKDLAQA TLRYANQVL HGREVSGTL 
      FEELIGIER TV
    CDS sequence
    • >TC032986 RA
      atgctagtg aacaaccga cggggtagg ttcattgtg 
      ttggggtcc tccggggaa tgtcttcca gtgacgaga 
      aaaccggtg gattcgcct ccgaaaagt gtttattcc 
      agctgtgaa tccagcgaa gaggagttt cttagtgcc 
      aagacgagt ctagacgat ggaagcgaa gacgaaaat 
      tttcacaag aggtacagt atcgatctg gagactccc 
      gagaggcgt cacactttt gcgcagaga ctcagagat 
      gcgttaagg agagaggtt tcagacact ttcagcagc 
      tcatctgag tcgagttat gctgtgggg atttcagtg 
      gccggctca gacgtgcaa gataagcaa attaaggtc 
      cgacgtgga ggttccacc gggtttgcg ctcaaggag 
      gactccctg gatgaaaat ttccttcaa gactcgttg 
      caacaagaa aagcaatgg atcgacaaa ttcaaaaac 
      aaacaatcc gttctgagc cgaaaagag tcaaacaag 
      tcgtccgaa tacagtttt agtaccgaa tacagttcc 
      gaattggaa gaagtttac gaacagttc tcgcgttgg 
      ctccaaaac ccgatcttg gaaacggaa aaaggaact 
      aagaaggag ttggaaggt cgcgatctg gctgtggtg 
      caattcgct ggatcgtta ttaaaacgt actctcagt 
      gaatccttc gctggggtt ccagtacca cttgcagaa 
      agcgctgat gcgacacga aacctcgat ttcaaccca 
      gttaagaaa aataagcta gtgctcagt gcaaaatcg 
      ctcagtttg gagctggcg cgacaaaaa cacaaacta 
      gcggctcaa ttaatttct cagctaacg cagaggtcg 
      gaaaatggg ctaaaacca gtcgcgaca ggaaattgg 
      ggttgtggc tcctccaag tgtggagat gtgcaacta 
      aaagtgata atccaatgg ttggcggca agtgtggcg 
      ggaatcccc tgccttaaa tactacact tgtggtcat 
      cagaaattg acaaaattg gatacagtg tgccgaata 
      ctcgttgat aggaaatgg actgttaag gatcttgct 
      caagctaca cttaggtac gcaaatcaa gtgctccac 
      ggacgtgaa gtcagtgga acacttttt gaggagtta 
      atcggtata gaacggacg gtttga
    • >TC032986 RB
      atgctagtg aacaaccga cggggtagg ttcattgtg 
      ttggggtcc tccggggaa tgtcttcca gtgacgaga 
      aaaccggtg gattcgcct ccgaaaagt gtttattcc 
      agctgtgaa tccagcgaa gaggagttt cttagtgcc 
      aagacgagt ctagacgat ggaagcgaa gacgaaaat 
      tttcacaag aggtacagt atcgatctg gagactccc 
      gagaggcgt cacactttt gcgcagaga ctcagagat 
      gcgttaagg agagaggtt tcagacact ttcagcagc 
      tcatctgag tcgagttat gctgtgggg atttcagtg 
      gccggctca gacgtgcaa gataagcaa attaaggtc 
      cgacgtgga ggttccacc gggtttgcg ctcaaggag 
      gactccctg gatgaaaat ttccttcaa gactcgttg 
      caacaagaa aagcaatgg atcgacaaa ttcaaaaac 
      aaacaatcc gttctgagc cgaaaagag tcaaacaag 
      tcgtccgaa tacagtttt agtaccgaa tacagttcc 
      gaattggaa gaagtttac gaacagttc tcgcgttgg 
      ctccaaaac ccgatcttg gaaacggaa aaaggaact 
      aagaaggag ttggaaggt cgcgatctg gctgtggtg 
      caattcgct ggatcgtta ttaaaacgt actctcagt 
      gaatccttc gctggggtt ccagtacca cttgcagaa 
      agcgctgat gcgacacga aacctcgat ttcaaccca 
      gttaagaaa aataagcta gtgctcagt gcaaaatcg 
      ctcagtttg gagctggcg cgacaaaaa cacaaacta 
      gcggctcaa ttaatgagt aaagagccc ttaacaaaa 
      gaaccggtt ttgagtgaa tgtaattta acaagtaaa 
      aagcgtaga gcttggttt atatcttgc gtaaatgaa 
      gccatcgtc caaacttta gaagagatt tcctgtagc 
      gttagttta cccaaagaa aaacagatt tctcagcta 
      acgcagagg tcggaaaat gggctaaaa ccagtcgcg 
      acaggaaat tggggttgt ggctcctcc aagtgtgga 
      gatgtgcaa ctaaaagtg ataatccaa tggttggcg 
      gcaagtgtg gcgggaatc ccctgcctt aaatactac 
      acttgtggt catcagaaa ttgacaaaa ttggataca 
      gtgtgccga atactcgtt gataggaaa tggactgtt 
      aaggatctt gctcaagct acacttagg tacgcaaat 
      caagtgctc cacggacgt gaagtcagt ggaacactt 
      tttgaggag ttaatcggt atagaacgg acggtttga
    mRNA sequence
    • >TC032986 RA
      ttaattaaa cactgatca ttcatcatt ttataccga 
      tcaaaatcg tattgatta ctgattgct ttattacaa 
      ttctttcca aaaccagct tcgagtcca gctcgaagt 
      cgagcgaaa tttgcagaa aaagggaca aattaacaa 
      cgaaatgct agtgaacaa ccgacgggg taggttcat 
      tgtgttggg gtcctccgg ggaatgtct tccagtgac 
      gagaaaacc ggtggattc gcctccgaa aagtgttta 
      ttccagctg tgaatccag cgaagagga gtttcttag 
      tgccaagac gagtctaga cgatggaag cgaagacga 
      aaattttca caagaggta cagtatcga tctggagac 
      tcccgagag gcgtcacac ttttgcgca gagactcag 
      agatgcgtt aaggagaga ggtttcaga cactttcag 
      cagctcatc tgagtcgag ttatgctgt ggggatttc 
      agtggccgg ctcagacgt gcaagataa gcaaattaa 
      ggtccgacg tggaggttc caccgggtt tgcgctcaa 
      ggaggactc cctggatga aaatttcct tcaagactc 
      gttgcaaca agaaaagca atggatcga caaattcaa 
      aaacaaaca atccgttct gagccgaaa agagtcaaa 
      caagtcgtc cgaatacag ttttagtac cgaatacag 
      ttccgaatt ggaagaagt ttacgaaca gttctcgcg 
      ttggctcca aaacccgat cttggaaac ggaaaaagg 
      aactaagaa ggagttgga aggtcgcga tctggctgt 
      ggtgcaatt cgctggatc gttattaaa acgtactct 
      cagtgaatc cttcgctgg ggttccagt accacttgc 
      agaaagcgc tgatgcgac acgaaacct cgatttcaa 
      cccagttaa gaaaaataa gctagtgct cagtgcaaa 
      atcgctcag tttggagct ggcgcgaca aaaacacaa 
      actagcggc tcaattaat ttctcagct aacgcagag 
      gtcggaaaa tgggctaaa accagtcgc gacaggaaa 
      ttggggttg tggctcctc caagtgtgg agatgtgca 
      actaaaagt gataatcca atggttggc ggcaagtgt 
      ggcgggaat cccctgcct taaatacta cacttgtgg 
      tcatcagaa attgacaaa attggatac agtgtgccg 
      aatactcgt tgataggaa atggactgt taaggatct 
      tgctcaagc tacacttag gtacgcaaa tcaagtgct 
      ccacggacg tgaagtcag tggaacact ttttgagga 
      gttaatcgg tatagaacg gacggtttg aggatgtct 
      agtcacact tcagttatt agcaaacac attcttata 
      actttttta ctttagata aagtgagct tccgcttgt 
      ttcaatgta cttgttttg ataaaaatg tgtaaggct 
      gttctgctc aaaccccac attagaata ttgtgagtt 
      gtaataacg aaaatatat gaaaatttg tgta
    • >TC032986 RB
      ttaattaaa cactgatca ttcatcatt ttataccga 
      tcaaaatcg tattgatta ctgattgct ttattacaa 
      ttctttcca aaaccagct tcgagtcca gctcgaagt 
      cgagcgaaa tttgcagaa aaagggaca aattaacaa 
      cgaaatgct agtgaacaa ccgacgggg taggttcat 
      tgtgttggg gtcctccgg ggaatgtct tccagtgac 
      gagaaaacc ggtggattc gcctccgaa aagtgttta 
      ttccagctg tgaatccag cgaagagga gtttcttag 
      tgccaagac gagtctaga cgatggaag cgaagacga 
      aaattttca caagaggta cagtatcga tctggagac 
      tcccgagag gcgtcacac ttttgcgca gagactcag 
      agatgcgtt aaggagaga ggtttcaga cactttcag 
      cagctcatc tgagtcgag ttatgctgt ggggatttc 
      agtggccgg ctcagacgt gcaagataa gcaaattaa 
      ggtccgacg tggaggttc caccgggtt tgcgctcaa 
      ggaggactc cctggatga aaatttcct tcaagactc 
      gttgcaaca agaaaagca atggatcga caaattcaa 
      aaacaaaca atccgttct gagccgaaa agagtcaaa 
      caagtcgtc cgaatacag ttttagtac cgaatacag 
      ttccgaatt ggaagaagt ttacgaaca gttctcgcg 
      ttggctcca aaacccgat cttggaaac ggaaaaagg 
      aactaagaa ggagttgga aggtcgcga tctggctgt 
      ggtgcaatt cgctggatc gttattaaa acgtactct 
      cagtgaatc cttcgctgg ggttccagt accacttgc 
      agaaagcgc tgatgcgac acgaaacct cgatttcaa 
      cccagttaa gaaaaataa gctagtgct cagtgcaaa 
      atcgctcag tttggagct ggcgcgaca aaaacacaa 
      actagcggc tcaattaat gagtaaaga gcccttaac 
      aaaagaacc ggttttgag tgaatgtaa tttaacaag 
      taaaaagcg tagagcttg gtttatatc ttgcgtaaa 
      tgaagccat cgtccaaac tttagaaga gatttcctg 
      tagcgttag tttacccaa agaaaaaca gatttctca 
      gctaacgca gaggtcgga aaatgggct aaaaccagt 
      cgcgacagg aaattgggg ttgtggctc ctccaagtg 
      tggagatgt gcaactaaa agtgataat ccaatggtt 
      ggcggcaag tgtggcggg aatcccctg ccttaaata 
      ctacacttg tggtcatca gaaattgac aaaattgga 
      tacagtgtg ccgaatact cgttgatag gaaatggac 
      tgttaagga tcttgctca agctacact taggtacgc 
      aaatcaagt gctccacgg acgtgaagt cagtggaac 
      actttttga ggagttaat cggtataga acggacggt 
      ttgaggatg tctagtcac acttcagtt attagcaaa 
      cacattctt ataactttt ttactttag ataaagtga 
      gcttccgct tgtttcaat gtacttgtt ttgataaaa 
      atgtgtaag gctgttctg ctcaaaccc cacattaga 
      atattgtga gttgtaata acgaaaata tatgaaaat 
      ttgtgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY