Status
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Gene information
General information
TC006857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 75861..94345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006857
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06099

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006857 RA
      MCDEAVLQN GALAPPKTA KQLEKEAKK QAKLDKLKQ 
      KLEKQQNAA PKKEAEKKE KKKEVKEAA VYNIATPEG 
      DKKDISGAL PDAYSPQFV EAAWYSWWE KQGFFKPEY 
      GRKSIAEPN PRGKFVIVI PPPNVTGSL HLGHALTNA 
      IQDSIVRWH RMRGETTLW NPGCDHAGI ATQVVVEKK 
      LWRNEKKTR HDIGREGFI SKVWEWKEE KGDRIYHQL 
      KKLGSSFDW DRAQFTMNP RLSRAVTEA FVRLHEEGY 
      IYRANRLVN WSCTLKSAI SDIEVDKIE IPGRTFLSV 
      PGYEEKIEF GVLVSFAYK IEGSDEKIV VATTRIETM 
      LGDTAVAVH PEDDRYKHL HGKRVIHPF CDRKLPIVL 
      DEFVERDFG TGAVKITPA HDPNDYEVG KRHSLPFIT 
      IFDDKGYIC GDYGKFTGM KRFEARKAV TEELKELGL 
      YVETKNNPM VVPVCSRSK DIIEPMLKP QWYVKCDEM 
      AAKAVEAVN SGELKIIPE MHIKTWHYW MNGIRDWCI 
      SRQLWWGHR IPAYYATLK SKGGKPGSD ADDNSHWIS 
      GRSESEARQ KAAKLYNTT PDDIILTQD EDVLDTWFS 
      SGLFPFSIF GWPEETEDL RIFYPTSLL ETGHDILFF 
      WVARMVFFG QKLLGKLPF KQVYLHPIV RDAHGRKMS 
      KSLGNVIDP MDVIKGIAL EDLHQQLYE SNLDPSEVE 
      KAKAGQKRD YPEGIPECG TDALRFALC AMCSGRDIN 
      LDILRVQGY RFFCNKIWN ATKFALTYF SDEFQAPVD 
      GGFVGNEKV MDLWMLSRL AAAVRDANH GFSHYEFPT 
      ATTAIYNVW LYELCDVYL EYLKPIFAS GDEATKKTA 
      QVTLYRALE VGLRLLSPF MPFITEELY QRLPRPKEI 
      RDKIPSICV CPYPEPEDC AWRNETLEH EVDFVQKVA 
      KAIRSARSD YNLPNKTKT EAYLQCSDS ATETTITKF 
      IQALQTLSY CSKIVVNEQ SPPGCAIIT VSDKCQVNL 
      LLKGLIEPA KEIAKIQKK LDFLQTTKS KLNQAISAP 
      DYATKVPPE VQQTNSEKL AQTAVELER LQSAIEALK 
      LME
    CDS sequence
    • >TC006857 RA
      atgtgtgac gaagcagtg ctccaaaat ggggctttg 
      gcccccccg aagacagcc aagcagttg gagaaagag 
      gccaaaaaa caggcaaag ttggacaag ttgaagcaa 
      aagttggag aagcagcaa aacgcggca ccgaagaag 
      gaggctgag aagaaagag aagaagaag gaggtgaag 
      gaggctgct gtttataat atagcgaca ccagagggt 
      gacaagaag gatatcagt ggggctttg cccgatgct 
      tatagcccc cagtttgtc gaggcggcg tggtactcc 
      tggtgggag aaacagggc tttttcaag ccggaatat 
      gggagaaag tcaatcgct gaacccaac cctagaggc 
      aaatttgta attgtaatt ccacctcca aacgtcacc 
      ggatcgctc catttaggt catgctttg accaatgcg 
      attcaagat tccattgtt cgatggcat agaatgaga 
      ggtgagaca actttgtgg aaccctggc tgtgatcat 
      gccggaatt gctactcaa gtggtagta gaaaaaaaa 
      ttatggcga aacgagaaa aaaacacga catgatatt 
      ggacgtgaa gggtttatt agcaaagtt tgggaatgg 
      aaagaggag aaaggcgac cggatttac caccagtta 
      aaaaaactg ggctcatcg ttcgattgg gaccgcgcc 
      caattcaca atgaacccc cgactgtcc cgtgccgta 
      accgaagcc ttcgtacgt ttacacgaa gaaggttac 
      atctaccgt gctaataga ctagtaaac tggtcttgc 
      actctcaaa tctgcaatt tccgacata gaagtcgac 
      aaaatcgaa attccagga cgcacattt ttatcagtc 
      cctggatac gaggagaaa atcgaattt ggggttttg 
      gtttcgttt gcttacaaa atcgaaggt tcggatgaa 
      aaaatcgta gtggccacg acccgaatc gagacgatg 
      ttgggcgat acggccgtg gcggtgcat cccgaagat 
      gaccgatat aagcatttg cacggcaaa cgggtgatt 
      cacccgttt tgcgaccgg aaattgccg attgtgctc 
      gatgagttt gttgagagg gatttcggg actggggct 
      gtgaaaatt acgccagcg catgatccg aatgattat 
      gaagtgggg aagagacac agtttgcct tttattacg 
      atttttgat gataaaggt tacatttgt ggggattac 
      gggaaattt acgggtatg aaacggttt gaggccagg 
      aaggctgta actgaagag ttgaaagag ttgggtttg 
      tatgttgag acgaagaat aatccaatg gttgtgcca 
      gtgtgtagt cgcagcaaa gatataatt gagccaatg 
      ttgaagcct cagtggtac gtcaagtgt gatgaaatg 
      gctgcgaaa gctgtagaa gcggtcaat tctggtgaa 
      ttgaagatt attcctgag atgcatatc aagacctgg 
      cattattgg atgaatggg atcagggat tggtgtatt 
      tccaggcag ttgtggtgg gggcatcga atccccgcc 
      tactatgcg acgttgaag agtaaaggg ggcaaaccg 
      ggatccgat gccgacgat aattcccat tggatttcc 
      ggccgcagt gaatccgaa gcccgtcaa aaagccgcc 
      aaactttac aacacgacc cctgacgac ataatcctc 
      actcaagac gaagacgtt cttgacacc tggttctcc 
      tccggtctc ttcccgttc tcaatcttc ggctggcct 
      gaagaaacg gaagactta cgcattttt taccccaca 
      tccctgctt gaaaccggc catgatatt ctcttcttc 
      tgggtggcc agaatggtg tttttcggc caaaaactc 
      ctcggaaaa cttcccttc aaacaggtg tacttacat 
      cccatcgtg cgtgacgca cacggacgc aaaatgagt 
      aaatcactg ggaaatgtg atcgatccg atggacgtc 
      atcaaggga atagccctt gaagactta catcaacag 
      ttgtatgaa agtaacttg gatccgagt gaagttgaa 
      aaagccaaa gctgggcaa aagcgcgac tatcctgag 
      ggaatcccc gaatgtggg acggacgca ctcagattc 
      gccttgtgt gccatgtgc tcagggcgt gatattaat 
      ttggatatt ttgcgagtg caaggatac agatttttc 
      tgtaataaa atctggaat gcgaccaag tttgcactg 
      acgtatttc agtgatgag tttcaagct ccggtagat 
      ggcgggttt gttggtaac gaaaaagtg atggatttg 
      tggatgttg agtcgattg gcggccgct gtcagggat 
      gccaaccac gggttttca cactatgag tttcccact 
      gccactacc gccatctac aatgtgtgg ttgtacgag 
      ctttgcgac gtctatttg gaatatttg aagccgata 
      tttgcgagt ggagacgaa gcgacgaag aaaactgcc 
      caagtcact ctgtatcgg gcgttggaa gtcggttta 
      cgccttctt tcacctttt atgcctttt attacggaa 
      gaactgtac cagaggttg cctcggcct aaagaaata 
      cgcgacaaa attccaagt atatgtgtg tgtccgtat 
      cccgagcct gaggattgt gcctggagg aacgaaacg 
      ttggaacat gaagttgat tttgtgcaa aaggtggcc 
      aaggcgatt aggtcggcg cggagtgat tataatctg 
      ccgaataag accaaaact gaagcttac ctccagtgc 
      agcgactca gcgaccgaa acaaccatc accaagttc 
      atccaagcg ctccaaacc ttgtcctac tgttcaaaa 
      attgtagtt aacgagcaa tcaccacca gggtgcgcc 
      atcatcacc gtttcggac aaatgtcaa gtcaatctg 
      ctcctcaaa ggattgatc gaaccggcc aaagaaatc 
      gccaagatc cagaagaaa ctcgacttt ttgcaaacc 
      acaaaatcc aaactgaac caggcgatt tctgcgccc 
      gattatgcg acaaaagtg ccaccagaa gtccaacaa 
      accaacagc gagaaattg gcgcagacg gctgtggaa 
      ctcgaacgg ttgcaaagt gcgatcgaa gctttaaaa 
      ttgatggaa tag
    mRNA sequence
    • >TC006857 RA
      gttggcttt tgacgtcac ttgcctgtc aaattacaa 
      tttcacaac gtgctctgt gttcacgaa accgtggac 
      gatgtgtga cgaagcagt gctccaaaa tggggcttt 
      ggccccccc gaagacagc caagcagtt ggagaaaga 
      ggccaaaaa acaggcaaa gttggacaa gttgaagca 
      aaagttgga gaagcagca aaacgcggc accgaagaa 
      ggaggctga gaagaaaga gaagaagaa ggaggtgaa 
      ggaggctgc tgtttataa tatagcgac accagaggg 
      tgacaagaa ggatatcag tggggcttt gcccgatgc 
      ttatagccc ccagtttgt cgaggcggc gtggtactc 
      ctggtggga gaaacaggg ctttttcaa gccggaata 
      tgggagaaa gtcaatcgc tgaacccaa ccctagagg 
      caaatttgt aattgtaat tccacctcc aaacgtcac 
      cggatcgct ccatttagg tcatgcttt gaccaatgc 
      gattcaaga ttccattgt tcgatggca tagaatgag 
      aggtgagac aactttgtg gaaccctgg ctgtgatca 
      tgccggaat tgctactca agtggtagt agaaaaaaa 
      attatggcg aaacgagaa aaaaacacg acatgatat 
      tggacgtga agggtttat tagcaaagt ttgggaatg 
      gaaagagga gaaaggcga ccggattta ccaccagtt 
      aaaaaaact gggctcatc gttcgattg ggaccgcgc 
      ccaattcac aatgaaccc ccgactgtc ccgtgccgt 
      aaccgaagc cttcgtacg tttacacga agaaggtta 
      catctaccg tgctaatag actagtaaa ctggtcttg 
      cactctcaa atctgcaat ttccgacat agaagtcga 
      caaaatcga aattccagg acgcacatt tttatcagt 
      ccctggata cgaggagaa aatcgaatt tggggtttt 
      ggtttcgtt tgcttacaa aatcgaagg ttcggatga 
      aaaaatcgt agtggccac gacccgaat cgagacgat 
      gttgggcga tacggccgt ggcggtgca tcccgaaga 
      tgaccgata taagcattt gcacggcaa acgggtgat 
      tcacccgtt ttgcgaccg gaaattgcc gattgtgct 
      cgatgagtt tgttgagag ggatttcgg gactggggc 
      tgtgaaaat tacgccagc gcatgatcc gaatgatta 
      tgaagtggg gaagagaca cagtttgcc ttttattac 
      gatttttga tgataaagg ttacatttg tggggatta 
      cgggaaatt tacgggtat gaaacggtt tgaggccag 
      gaaggctgt aactgaaga gttgaaaga gttgggttt 
      gtatgttga gacgaagaa taatccaat ggttgtgcc 
      agtgtgtag tcgcagcaa agatataat tgagccaat 
      gttgaagcc tcagtggta cgtcaagtg tgatgaaat 
      ggctgcgaa agctgtaga agcggtcaa ttctggtga 
      attgaagat tattcctga gatgcatat caagacctg 
      gcattattg gatgaatgg gatcaggga ttggtgtat 
      ttccaggca gttgtggtg ggggcatcg aatccccgc 
      ctactatgc gacgttgaa gagtaaagg gggcaaacc 
      gggatccga tgccgacga taattccca ttggatttc 
      cggccgcag tgaatccga agcccgtca aaaagccgc 
      caaacttta caacacgac ccctgacga cataatcct 
      cactcaaga cgaagacgt tcttgacac ctggttctc 
      ctccggtct cttcccgtt ctcaatctt cggctggcc 
      tgaagaaac ggaagactt acgcatttt ttaccccac 
      atccctgct tgaaaccgg ccatgatat tctcttctt 
      ctgggtggc cagaatggt gtttttcgg ccaaaaact 
      cctcggaaa acttccctt caaacaggt gtacttaca 
      tcccatcgt gcgtgacgc acacggacg caaaatgag 
      taaatcact gggaaatgt gatcgatcc gatggacgt 
      catcaaggg aatagccct tgaagactt acatcaaca 
      gttgtatga aagtaactt ggatccgag tgaagttga 
      aaaagccaa agctgggca aaagcgcga ctatcctga 
      gggaatccc cgaatgtgg gacggacgc actcagatt 
      cgccttgtg tgccatgtg ctcagggcg tgatattaa 
      tttggatat tttgcgagt gcaaggata cagattttt 
      ctgtaataa aatctggaa tgcgaccaa gtttgcact 
      gacgtattt cagtgatga gtttcaagc tccggtaga 
      tggcgggtt tgttggtaa cgaaaaagt gatggattt 
      gtggatgtt gagtcgatt ggcggccgc tgtcaggga 
      tgccaacca cgggttttc acactatga gtttcccac 
      tgccactac cgccatcta caatgtgtg gttgtacga 
      gctttgcga cgtctattt ggaatattt gaagccgat 
      atttgcgag tggagacga agcgacgaa gaaaactgc 
      ccaagtcac tctgtatcg ggcgttgga agtcggttt 
      acgccttct ttcaccttt tatgccttt tattacgga 
      agaactgta ccagaggtt gcctcggcc taaagaaat 
      acgcgacaa aattccaag tatatgtgt gtgtccgta 
      tcccgagcc tgaggattg tgcctggag gaacgaaac 
      gttggaaca tgaagttga ttttgtgca aaaggtggc 
      caaggcgat taggtcggc gcggagtga ttataatct 
      gccgaataa gaccaaaac tgaagctta cctccagtg 
      cagcgactc agcgaccga aacaaccat caccaagtt 
      catccaagc gctccaaac cttgtccta ctgttcaaa 
      aattgtagt taacgagca atcaccacc agggtgcgc 
      catcatcac cgtttcgga caaatgtca agtcaatct 
      gctcctcaa aggattgat cgaaccggc caaagaaat 
      cgccaagat ccagaagaa actcgactt tttgcaaac 
      cacaaaatc caaactgaa ccaggcgat ttctgcgcc 
      cgattatgc gacaaaagt gccaccaga agtccaaca 
      aaccaacag cgagaaatt ggcgcagac ggctgtgga 
      actcgaacg gttgcaaag tgcgatcga agctttaaa 
      attgatgga atagaagtg atttaattt atttcggtt 
      tatttattg tagctttta ggcggtgat tgtaaaata 
      cggtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY