Status
Please wait ...
Gene information
General information
TC010229 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450254.1: 1475..4130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010229
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06097

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    pupal molt not completely molted - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010229 RA
      MDKHIQTII KGGKEAAES LQCFVEEAS QVFEFPELN 
      KNNQRKELW EALFTLAKT ANSEITLMN CFIAIRILS 
      REKMGLNEL ITDEWFKLM QKHSGLDDP QYKFDASKL 
      LLAIEVEKC LCNVIFNSP VAAGLCCKN GVLEQLTER 
      IKNPQENKI PEEIRFYDV KLIFLLTAL CSEVRSKVK 
      EELKGVESL LKMLDEMLK EAAQASPEK IILDDQKVD 
      LACEILKAL FNVTLSYGI YDDKEENEQ CDHLVTLLR 
      MYLLVPTQD KEKGLALKN NVINLLTNM PDSTFKNLI 
      TPVQPGQNL KPKFQFEGQ NMTAFYEIL MVLKSKFNN 
      VEGVTKQYE ILCPVVTIL LKSANSERA IRKYLRLQI 
      LPPLKDVHS RPEQGSTLR NHLCRLLTT PITQLRDLV 
      ADLLFILCK KSVKRMIKY TGYGNAAGL FAKRGLLGG 
      RSDRGEADY SSGSEGSET EEYAEFKHG INPVIGCYE 
      EPHPNAMEN MTEEQKEYE AMKLVELMD SLTRSGTIQ 
      PCRVGEDGK PHPIKHVLQ LQEGIKAQQ VNNDSDSDD
    CDS sequence
    • >TC010229 RA
      atggataaa catatccaa actataata aaaggagga 
      aaagaagca gcagaatcg ctacaatgt ttcgttgaa 
      gaggcttca caagtattc gagtttcca gaactcaat 
      aaaaataac caacggaaa gaattatgg gaggcatta 
      tttacattg gccaaaaca gccaatagt gagattaca 
      ctaatgaat tgctttatc gcaattcgt atattaagt 
      cgggaaaaa atgggtctg aacgagtta ataacagac 
      gaatggttc aaactcatg caaaaacac tccggtttg 
      gacgatccc cagtacaaa tttgatgct agtaaatta 
      ttattggca attgaagtg gaaaaatgt ttatgcaat 
      gtgattttc aacagtcct gttgctgca ggactgtgt 
      tgcaaaaat ggtgttttg gaacagtta actgaacgt 
      atcaaaaat ccccaagag aataaaata ccggaagaa 
      atcagattt tacgatgtt aagctcatt ttcctacta 
      acagcactg tgttctgaa gttcgctca aaagtaaag 
      gaagaatta aaaggggtc gaatctttg cttaaaatg 
      ctagatgag atgctgaaa gaagcggcc caagcatca 
      cccgaaaaa attattttg gatgaccaa aaagtggac 
      ctggcttgc gaaattctg aaagcccta tttaacgta 
      acattaagt tacggcatc tatgacgat aaagaagaa 
      aacgaacag tgtgaccat ttggtcact ctcttaaga 
      atgtatctt cttgtacca acacaagat aaagaaaaa 
      ggcctggca ttaaaaaac aacgtaatc aatttacta 
      acaaacatg cctgattca actttcaaa aatctgata 
      acacctgtc caacctggc caaaacctc aaacccaaa 
      tttcaattc gaaggccag aatatgacg gctttttac 
      gaaatcctg atggtgcta aaaagcaaa tttaacaac 
      gttgaaggt gtcacaaaa caatacgaa attttgtgt 
      cctgtcgta acaatttta ttgaaaagt gcaaacagt 
      gaacgtgct attcgaaaa tatctccgt ctccaaatt 
      cttccacct ttgaaagat gtgcacagt cgacctgaa 
      caaggctcc acacttcgt aatcacttg tgtcggctt 
      ttaaccacg ccgataaca cagttgagg gatttggtg 
      gccgatttg ttgttcatt ttgtgcaaa aaaagcgtc 
      aaaaggatg attaagtat acgggttat ggcaatgct 
      gcgggtctc tttgccaaa agggggctc ttagggggg 
      cgctcagat agaggcgag gctgattat agttcaggt 
      agtgaggga agcgaaact gaagaatat gccgaattt 
      aaacatggg attaatcca gtgattggg tgttacgaa 
      gaaccgcat cccaacgca atggaaaat atgacagag 
      gaacagaaa gagtatgag gccatgaag ttggtcgaa 
      ttaatggac agtttgacc agatctggg accatccaa 
      ccatgtaga gtaggcgag gatgggaaa cctcatccc 
      atcaaacat gtgttacaa ttgcaggaa ggtataaaa 
      gcgcaacaa gtcaacaat gacagcgat agcgatgat 
      taa
    mRNA sequence
    • >TC010229 RA
      caggtgtca aaactgtca atttctcta attttatct 
      gtggtgttt agcagaaaa acataattt gtgtgtaaa 
      caacaagaa aacattcaa taaaaccgt aacgaaata 
      ttcaggtgt tgaataata atggataaa catatccaa 
      actataata aaaggagga aaagaagca gcagaatcg 
      ctacaatgt ttcgttgaa gaggcttca caagtattc 
      gagtttcca gaactcaat aaaaataac caacggaaa 
      gaattatgg gaggcatta tttacattg gccaaaaca 
      gccaatagt gagattaca ctaatgaat tgctttatc 
      gcaattcgt atattaagt cgggaaaaa atgggtctg 
      aacgagtta ataacagac gaatggttc aaactcatg 
      caaaaacac tccggtttg gacgatccc cagtacaaa 
      tttgatgct agtaaatta ttattggca attgaagtg 
      gaaaaatgt ttatgcaat gtgattttc aacagtcct 
      gttgctgca ggactgtgt tgcaaaaat ggtgttttg 
      gaacagtta actgaacgt atcaaaaat ccccaagag 
      aataaaata ccggaagaa atcagattt tacgatgtt 
      aagctcatt ttcctacta acagcactg tgttctgaa 
      gttcgctca aaagtaaag gaagaatta aaaggggtc 
      gaatctttg cttaaaatg ctagatgag atgctgaaa 
      gaagcggcc caagcatca cccgaaaaa attattttg 
      gatgaccaa aaagtggac ctggcttgc gaaattctg 
      aaagcccta tttaacgta acattaagt tacggcatc 
      tatgacgat aaagaagaa aacgaacag tgtgaccat 
      ttggtcact ctcttaaga atgtatctt cttgtacca 
      acacaagat aaagaaaaa ggcctggca ttaaaaaac 
      aacgtaatc aatttacta acaaacatg cctgattca 
      actttcaaa aatctgata acacctgtc caacctggc 
      caaaacctc aaacccaaa tttcaattc gaaggccag 
      aatatgacg gctttttac gaaatcctg atggtgcta 
      aaaagcaaa tttaacaac gttgaaggt gtcacaaaa 
      caatacgaa attttgtgt cctgtcgta acaatttta 
      ttgaaaagt gcaaacagt gaacgtgct attcgaaaa 
      tatctccgt ctccaaatt cttccacct ttgaaagat 
      gtgcacagt cgacctgaa caaggctcc acacttcgt 
      aatcacttg tgtcggctt ttaaccacg ccgataaca 
      cagttgagg gatttggtg gccgatttg ttgttcatt 
      ttgtgcaaa aaaagcgtc aaaaggatg attaagtat 
      acgggttat ggcaatgct gcgggtctc tttgccaaa 
      agggggctc ttagggggg cgctcagat agaggcgag 
      gctgattat agttcaggt agtgaggga agcgaaact 
      gaagaatat gccgaattt aaacatggg attaatcca 
      gtgattggg tgttacgaa gaaccgcat cccaacgca 
      atggaaaat atgacagag gaacagaaa gagtatgag 
      gccatgaag ttggtcgaa ttaatggac agtttgacc 
      agatctggg accatccaa ccatgtaga gtaggcgag 
      gatgggaaa cctcatccc atcaaacat gtgttacaa 
      ttgcaggaa ggtataaaa gcgcaacaa gtcaacaat 
      gacagcgat agcgatgat taatttatt tttaataaa 
      taataaatg tcgtaaacc tagtctgca ttttaaaat 
      gattgaatc aaatcgttt actattcta ttgtttgta 
      tttttaacg aaacgatgt taggaaaaa tagtgagat 
      tatttcttt atgtacgta ttttattta atattattt 
      atgttgtga tacttccgt tttaatgat cgaataaag 
      tcgcactga atgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY