Status
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Gene information
General information
TC010229 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450254.1: 1475..4130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010229
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06097

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010229 RA
      MDKHIQTII KGGKEAAES LQCFVEEAS QVFEFPELN 
KNNQRKELW EALFTLAKT ANSEITLMN CFIAIRILS 
REKMGLNEL ITDEWFKLM QKHSGLDDP QYKFDASKL 
LLAIEVEKC LCNVIFNSP VAAGLCCKN GVLEQLTER 
IKNPQENKI PEEIRFYDV KLIFLLTAL CSEVRSKVK 
EELKGVESL LKMLDEMLK EAAQASPEK IILDDQKVD 
LACEILKAL FNVTLSYGI YDDKEENEQ CDHLVTLLR 
MYLLVPTQD KEKGLALKN NVINLLTNM PDSTFKNLI 
TPVQPGQNL KPKFQFEGQ NMTAFYEIL MVLKSKFNN 
VEGVTKQYE ILCPVVTIL LKSANSERA IRKYLRLQI 
LPPLKDVHS RPEQGSTLR NHLCRLLTT PITQLRDLV 
ADLLFILCK KSVKRMIKY TGYGNAAGL FAKRGLLGG 
RSDRGEADY SSGSEGSET EEYAEFKHG INPVIGCYE 
EPHPNAMEN MTEEQKEYE AMKLVELMD SLTRSGTIQ 
PCRVGEDGK PHPIKHVLQ LQEGIKAQQ VNNDSDSDD
    CDS sequence
    • >TC010229 RA
      atggataaa catatccaa actataata aaaggagga 
aaagaagca gcagaatcg ctacaatgt ttcgttgaa 
gaggcttca caagtattc gagtttcca gaactcaat 
aaaaataac caacggaaa gaattatgg gaggcatta 
tttacattg gccaaaaca gccaatagt gagattaca 
ctaatgaat tgctttatc gcaattcgt atattaagt 
cgggaaaaa atgggtctg aacgagtta ataacagac 
gaatggttc aaactcatg caaaaacac tccggtttg 
gacgatccc cagtacaaa tttgatgct agtaaatta 
ttattggca attgaagtg gaaaaatgt ttatgcaat 
gtgattttc aacagtcct gttgctgca ggactgtgt 
tgcaaaaat ggtgttttg gaacagtta actgaacgt 
atcaaaaat ccccaagag aataaaata ccggaagaa 
atcagattt tacgatgtt aagctcatt ttcctacta 
acagcactg tgttctgaa gttcgctca aaagtaaag 
gaagaatta aaaggggtc gaatctttg cttaaaatg 
ctagatgag atgctgaaa gaagcggcc caagcatca 
cccgaaaaa attattttg gatgaccaa aaagtggac 
ctggcttgc gaaattctg aaagcccta tttaacgta 
acattaagt tacggcatc tatgacgat aaagaagaa 
aacgaacag tgtgaccat ttggtcact ctcttaaga 
atgtatctt cttgtacca acacaagat aaagaaaaa 
ggcctggca ttaaaaaac aacgtaatc aatttacta 
acaaacatg cctgattca actttcaaa aatctgata 
acacctgtc caacctggc caaaacctc aaacccaaa 
tttcaattc gaaggccag aatatgacg gctttttac 
gaaatcctg atggtgcta aaaagcaaa tttaacaac 
gttgaaggt gtcacaaaa caatacgaa attttgtgt 
cctgtcgta acaatttta ttgaaaagt gcaaacagt 
gaacgtgct attcgaaaa tatctccgt ctccaaatt 
cttccacct ttgaaagat gtgcacagt cgacctgaa 
caaggctcc acacttcgt aatcacttg tgtcggctt 
ttaaccacg ccgataaca cagttgagg gatttggtg 
gccgatttg ttgttcatt ttgtgcaaa aaaagcgtc 
aaaaggatg attaagtat acgggttat ggcaatgct 
gcgggtctc tttgccaaa agggggctc ttagggggg 
cgctcagat agaggcgag gctgattat agttcaggt 
agtgaggga agcgaaact gaagaatat gccgaattt 
aaacatggg attaatcca gtgattggg tgttacgaa 
gaaccgcat cccaacgca atggaaaat atgacagag 
gaacagaaa gagtatgag gccatgaag ttggtcgaa 
ttaatggac agtttgacc agatctggg accatccaa 
ccatgtaga gtaggcgag gatgggaaa cctcatccc 
atcaaacat gtgttacaa ttgcaggaa ggtataaaa 
gcgcaacaa gtcaacaat gacagcgat agcgatgat 
taa
    mRNA sequence
    • >TC010229 RA
      caggtgtca aaactgtca atttctcta attttatct 
gtggtgttt agcagaaaa acataattt gtgtgtaaa 
caacaagaa aacattcaa taaaaccgt aacgaaata 
ttcaggtgt tgaataata atggataaa catatccaa 
actataata aaaggagga aaagaagca gcagaatcg 
ctacaatgt ttcgttgaa gaggcttca caagtattc 
gagtttcca gaactcaat aaaaataac caacggaaa 
gaattatgg gaggcatta tttacattg gccaaaaca 
gccaatagt gagattaca ctaatgaat tgctttatc 
gcaattcgt atattaagt cgggaaaaa atgggtctg 
aacgagtta ataacagac gaatggttc aaactcatg 
caaaaacac tccggtttg gacgatccc cagtacaaa 
tttgatgct agtaaatta ttattggca attgaagtg 
gaaaaatgt ttatgcaat gtgattttc aacagtcct 
gttgctgca ggactgtgt tgcaaaaat ggtgttttg 
gaacagtta actgaacgt atcaaaaat ccccaagag 
aataaaata ccggaagaa atcagattt tacgatgtt 
aagctcatt ttcctacta acagcactg tgttctgaa 
gttcgctca aaagtaaag gaagaatta aaaggggtc 
gaatctttg cttaaaatg ctagatgag atgctgaaa 
gaagcggcc caagcatca cccgaaaaa attattttg 
gatgaccaa aaagtggac ctggcttgc gaaattctg 
aaagcccta tttaacgta acattaagt tacggcatc 
tatgacgat aaagaagaa aacgaacag tgtgaccat 
ttggtcact ctcttaaga atgtatctt cttgtacca 
acacaagat aaagaaaaa ggcctggca ttaaaaaac 
aacgtaatc aatttacta acaaacatg cctgattca 
actttcaaa aatctgata acacctgtc caacctggc 
caaaacctc aaacccaaa tttcaattc gaaggccag 
aatatgacg gctttttac gaaatcctg atggtgcta 
aaaagcaaa tttaacaac gttgaaggt gtcacaaaa 
caatacgaa attttgtgt cctgtcgta acaatttta 
ttgaaaagt gcaaacagt gaacgtgct attcgaaaa 
tatctccgt ctccaaatt cttccacct ttgaaagat 
gtgcacagt cgacctgaa caaggctcc acacttcgt 
aatcacttg tgtcggctt ttaaccacg ccgataaca 
cagttgagg gatttggtg gccgatttg ttgttcatt 
ttgtgcaaa aaaagcgtc aaaaggatg attaagtat 
acgggttat ggcaatgct gcgggtctc tttgccaaa 
agggggctc ttagggggg cgctcagat agaggcgag 
gctgattat agttcaggt agtgaggga agcgaaact 
gaagaatat gccgaattt aaacatggg attaatcca 
gtgattggg tgttacgaa gaaccgcat cccaacgca 
atggaaaat atgacagag gaacagaaa gagtatgag 
gccatgaag ttggtcgaa ttaatggac agtttgacc 
agatctggg accatccaa ccatgtaga gtaggcgag 
gatgggaaa cctcatccc atcaaacat gtgttacaa 
ttgcaggaa ggtataaaa gcgcaacaa gtcaacaat 
gacagcgat agcgatgat taatttatt tttaataaa 
taataaatg tcgtaaacc tagtctgca ttttaaaat 
gattgaatc aaatcgttt actattcta ttgtttgta 
tttttaacg aaacgatgt taggaaaaa tagtgagat 
tatttcttt atgtacgta ttttattta atattattt 
atgttgtga tacttccgt tttaatgat cgaataaag 
tcgcactga atgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY