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Gene information
General information
TC002262 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 691018..700135
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002262
    iBeetle screen iB_06090

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002262 RA
      MIVYHAYLK YVAFAVATV TFPIIGLIV LKIIQKFTT 
      GRCKSLVCL NGKTAIVTG GGSGIGFET ALQLASRGC 
      RVIIADRED ATKTKNKII DLTNNTNIE TKHLDLTSF 
      QSIRRFAKE INETEDRLD ILINNAGAG SVGNKHTDD 
      GLHITMQVN HFGPFLLTH LLADLLKKS APSRIIFVS 
      SATAFFWNN LTVKNLNYP SDHPMTFFR SSFIYGNSK 
      LCNIIAANG FAERLSQFG VTSNSLHPG LVNTEIHLK 
      SARILGIET FGKLFANTI LYLYGKSPK EGAQTTIAL 
      AVSNHLQNV TGKHFWDCR TFIQPPKAW NRKFCTEIW 
      EKSEELTKL KPEEKL
    CDS sequence
    • >TC002262 RA
      atgattgtc tatcacgcg tatctcaaa tatgtcgct 
      tttgcggta gctacagtc acttttccc atcatagga 
      ttgattgtt ttgaagatt attcaaaag tttacgacc 
      ggcaggtgc aagagtttg gtttgtttg aatggaaag 
      acggcgatt gttacggga ggtggttca ggaattggc 
      tttgaaacg gctctccaa ctggcctcc cgcggctgt 
      cgagtaata atcgcagac cgagaagac gccaccaaa 
      actaaaaac aaaatcatt gaccttacc aacaacaca 
      aacatcgaa acaaaacat ctcgaccta acttccttc 
      cagtcaatt cgacgcttt gctaaagaa attaacgaa 
      actgaagat cgactcgac attttaata aacaatgct 
      ggagcagga tcagttgga aacaaacac actgacgat 
      ggcctgcac atcaccatg caagtcaac cactttggt 
      ccattcctt ttaacacat ttattggcc gatttgttg 
      aaaaaatcc gctccaagt agaattatt ttcgtgagc 
      tcggccaca gctttcttc tggaacaat ctaacagta 
      aagaatttg aattaccca agtgaccat ccgatgact 
      ttcttccga tcatctttc atttatggc aactcaaaa 
      ttgtgcaac atcattgcg gccaatggc tttgccgaa 
      aggttgagc caatttggt gttacgtct aactcactc 
      catccgggt cttgtcaac actgaaatt cacctcaaa 
      tcggccagg attttggga attgaaacg tttggaaaa 
      ttattcgca aatacgatt ttgtatttg tacggcaaa 
      agccccaaa gaaggtgcc caaaccacc atagccctg 
      gccgtctca aaccatttg caaaacgtg accgggaaa 
      cacttctgg gattgccgt actttcatc caacctccg 
      aaagcttgg aacaggaag ttttgtacc gaaatttgg 
      gaaaaaagc gaagagttg accaaactt aagcccgag 
      gagaagctt taa
    mRNA sequence
    • >TC002262 RA
      tcagatttt cgaagatga ttgtctatc acgcgtatc 
      tcaaatatg tcgcttttg cggtagcta cagtcactt 
      ttcccatca taggattga ttgttttga agattattc 
      aaaagttta cgaccggca ggtgcaaga gtttggttt 
      gtttgaatg gaaagacgg cgattgtta cgggaggtg 
      gttcaggaa ttggctttg aaacggctc tccaactgg 
      cctcccgcg gctgtcgag taataatcg cagaccgag 
      aagacgcca ccaaaacta aaaacaaaa tcattgacc 
      ttaccaaca acacaaaca tcgaaacaa aacatctcg 
      acctaactt ccttccagt caattcgac gctttgcta 
      aagaaatta acgaaactg aagatcgac tcgacattt 
      taataaaca atgctggag caggatcag ttggaaaca 
      aacacactg acgatggcc tgcacatca ccatgcaag 
      tcaaccact ttggtccat tccttttaa cacatttat 
      tggccgatt tgttgaaaa aatccgctc caagtagaa 
      ttattttcg tgagctcgg ccacagctt tcttctgga 
      acaatctaa cagtaaaga atttgaatt acccaagtg 
      accatccga tgactttct tccgatcat ctttcattt 
      atggcaact caaaattgt gcaacatca ttgcggcca 
      atggctttg ccgaaaggt tgagccaat ttggtgtta 
      cgtctaact cactccatc cgggtcttg tcaacactg 
      aaattcacc tcaaatcgg ccaggattt tgggaattg 
      aaacgtttg gaaaattat tcgcaaata cgattttgt 
      atttgtacg gcaaaagcc ccaaagaag gtgcccaaa 
      ccaccatag ccctggccg tctcaaacc atttgcaaa 
      acgtgaccg ggaaacact tctgggatt gccgtactt 
      tcatccaac ctccgaaag cttggaaca ggaagtttt 
      gtaccgaaa tttgggaaa aaagcgaag agttgacca 
      aacttaagc ccgaggaga agctttaat aataatttt 
      cgtcaaggc tataacaac tttttcttt ttcgatgta 
      ttgtttgtt aatttgatc ccttggtga ggcattaaa 
      atatcgcaa agatgacac agacagcgc ggctaatgg 
      tctcaaaca tcgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY