| General information | |||
| TC031683 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 412454..442797 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031683 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06089 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax fused - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC031683 RA
MYRVILLLW AHVIVQGSS EVMLEPNMT TFMRTVDGF SNQLLGVTS GKIVITGNN TLHLTNFSF DGKVSDAHF IVGKGTVSE NGTFVPFSE SSDNLPPLD NEDIDLTLP NNLTVIDID WLAIFSPTT QENLGFVLL TKTVQSSPQ TFSFDNDVI SGPVLLLDK KTLFIPNFR YKGDHKNVR FWVGNGSEP SSDGIALSD EKNSSESLG PYQGQNVKL HLPESLSTD NIDYLAVWS IDEAVSLGY VPIKGVSEA PEVEQETKR LSEGNFEVI VPKCCSSSA VMTSRGCEE KGRLQIFVP NFNIYEHNA THFRNESLD LNKVDFKIY DSTMKCPAG KFPLDQSSE EFALLINGS LLIKGAAGK GIWSQENFC LDNVVDESN VTQMRIFVC VPEVQIQKA MCYTYITFT IISMLCLAA TFFIYFFLS HIEDAHRIL FTAYVFDMA MVFISLTVV QVGHVEGSG CSVCGFIFQ FFMVSSFVM LAILCVDIC IIIKYIDNN IKPRKRKVW SLGVGFLVP LFFLIISLI RNLTPDVPN SFFKPQYQT NACYFEAED QRVSIFYVP IIFSMIVSG ISLGVAKYI IVRYDRLRL NDFFWIENG TKYKFMWRK HLLVLIITS TLRIFDLVL YLSYGHDSM LLVVDIFEA TQGIFILLL FVLNEQTRK EIRKSIEKR RRRRRKHHL NHSKEEELV MSKPT
- >TC031683 RB
MYRVILLLW AHVIVQGSS EVMLEPNMT TFMRTVDGF SNQLLGVTS GKIVITGNN TLHLTNFSF DGKVSDAHF IVGKGTVSE NGTFVPFSE SSDNLPPLD NEDIDLTLP NNLTVIDID WLAIFSPTT QENLGFVLL TKTVQSSPQ TFSFDNDVI SGPVLLLDK KTLFIPNFR YKGDHKNVR FWVGNGSEP SSDGIALSD EKNSSESLG PYQGQNVKL HLPESLSTD NIDYLAVWS IDEAVSLGY VPIKGVSEA PEVEQETKR LSEGNFEVI VPKCCSSSA VMTSRGCEE KGRLQIFVP NFNIYEHNA THFRNESLD LNKVDFKIY DSTMKCPAG KFPLDQSSE EFALLINGS LLIKGAAGK GIWSQENFC LDNVVDESN VTQMRIFVC VPEVQIQKA MCYTYITFT IISMLCLAA TFFIYFFLS HIEDAHRIL FTAYVFDMA MVFISLTVV QVGHVEGSG CSVCGFIFQ FFMVSSFVM LAILCVDIC IIIKYIDNN IKPRKRKVW SLGVGFLVP LFFLIISLI RNLTPDVPN SFFKPQYQT NACYFEAED QRVSIFYVP IIFSMIVSG ISLGVAKYI IVRYDRLRL NDFFWIENG TKYKFMFRQ CFITSVIMT ISWISNVLM SFLDESSGI LKAAVFFEA LQGVLIVAV FTMHHYGTA YLETRRQRT EPPVEMNPL SQNNHE
- >TC031683 RA
atgtatcga gtaattcta cttttgtgg gcacatgtt attgtgcaa ggatctagc gaggttatg cttgaaccg aacatgacg acatttatg cgaacagtg gacggtttt tctaatcag ttattaggt gtgacttcc ggaaaaatc gtgataacc ggaaacaac actcttcac ctaaccaat ttctccttc gatggtaaa gtgtccgac gcccatttt atagtcggc aaaggcaca gtttccgaa aacggaact ttcgtacct ttctctgaa agttccgac aatcttccg ccattagac aacgaagac atcgactta actctgcct aataatcta acagtcatt gatatcgat tggttggca attttttct ccaacgacc caggaaaac ctaggcttt gtattgcta acaaaaact gtacagtca tcacctcaa actttcagc ttcgacaat gatgttatt tccggtcct gtattatta ctcgataaa aagacactc tttatcccg aattttcgc tacaaaggc gatcacaaa aatgtgcgc ttttgggtg ggcaatggc agcgaaccg tcctcggac ggaatcgct ctgtcagac gagaaaaac tcttcggaa agtcttggg ccatatcag ggacaaaat gtgaagctt catttacca gaaagtctg tcaactgac aatatcgac tatttggct gtttggtca atcgacgag gcagttagt ctaggctat gttcccatc aaaggagtt agcgaagcg ccagaagtt gaacaggaa acaaaaaga ttaagtgaa gggaacttc gaagtgata gtaccaaag tgttgctcc tcttcagct gtaatgacc agtcgcgga tgtgaggaa aagggtcgc ttacaaata tttgtacca aactttaac atctacgaa cacaatgca acacatttc aggaatgaa tctctggac ctaaacaag gtggatttt aaaatatac gattccaca atgaagtgt cctgctgga aaatttcct ttagaccag tcaagtgaa gaattcgct ctactgatt aatggctct ttacttatt aagggggca gccgggaaa ggcatatgg tcacaggaa aacttttgt ttagacaac gtcgtagat gagagtaat gtaacacaa atgagaatt tttgtatgc gtcccggaa gtccaaata cagaaagca atgtgctac acttacata actttcaca attatctcg atgctttgc cttgctgct actttcttc atatacttc ttcttgtca catatagaa gatgcccac agaatcctg tttactgct tatgttttt gatatggct atggttttt atttccttg actgtagtt caagtggga catgtggaa ggcagtgga tgttcagtt tgtggtttt attttccag ttcttcatg gtttcgtct tttgtaatg ttggccatt ttatgtgta gacatttgc atcattatt aagtacatt gacaacaat attaaacca cgaaaacgg aaagtttgg tcacttggt gttggcttt ttggttccg ttatttttc ttaatcatc tcattaatt cgaaatctc acaccagat gtaccaaac tcgtttttc aaacctcaa tatcaaact aatgcctgc tactttgaa gctgaagac caaagagtt tcaattttt tatgttcct attattttc tcaatgatc gtaagtggc atttccctg ggtgtggca aaatacata atcgtgcga tacgaccga ttaagatta aacgatttc ttttggatc gaaaacgga acgaaatac aaattcatg tggcgaaaa catttattg gttcttatt ataacgtca acgttacgg atttttgat ctagtgctt tatttaagt tatggacat gatagtatg ttattagta gttgatata tttgaggcg actcaagga atttttatt ctattacta ttcgttctg aatgaacaa acaagaaaa gagattcga aaaagtatc gaaaaaagg agaagaaga agacggaag catcatcta aaccattca aaagaagag gaacttgtg atgtcaaag ccgacctga
- >TC031683 RB
atgtatcga gtaattcta cttttgtgg gcacatgtt attgtgcaa ggatctagc gaggttatg cttgaaccg aacatgacg acatttatg cgaacagtg gacggtttt tctaatcag ttattaggt gtgacttcc ggaaaaatc gtgataacc ggaaacaac actcttcac ctaaccaat ttctccttc gatggtaaa gtgtccgac gcccatttt atagtcggc aaaggcaca gtttccgaa aacggaact ttcgtacct ttctctgaa agttccgac aatcttccg ccattagac aacgaagac atcgactta actctgcct aataatcta acagtcatt gatatcgat tggttggca attttttct ccaacgacc caggaaaac ctaggcttt gtattgcta acaaaaact gtacagtca tcacctcaa actttcagc ttcgacaat gatgttatt tccggtcct gtattatta ctcgataaa aagacactc tttatcccg aattttcgc tacaaaggc gatcacaaa aatgtgcgc ttttgggtg ggcaatggc agcgaaccg tcctcggac ggaatcgct ctgtcagac gagaaaaac tcttcggaa agtcttggg ccatatcag ggacaaaat gtgaagctt catttacca gaaagtctg tcaactgac aatatcgac tatttggct gtttggtca atcgacgag gcagttagt ctaggctat gttcccatc aaaggagtt agcgaagcg ccagaagtt gaacaggaa acaaaaaga ttaagtgaa gggaacttc gaagtgata gtaccaaag tgttgctcc tcttcagct gtaatgacc agtcgcgga tgtgaggaa aagggtcgc ttacaaata tttgtacca aactttaac atctacgaa cacaatgca acacatttc aggaatgaa tctctggac ctaaacaag gtggatttt aaaatatac gattccaca atgaagtgt cctgctgga aaatttcct ttagaccag tcaagtgaa gaattcgct ctactgatt aatggctct ttacttatt aagggggca gccgggaaa ggcatatgg tcacaggaa aacttttgt ttagacaac gtcgtagat gagagtaat gtaacacaa atgagaatt tttgtatgc gtcccggaa gtccaaata cagaaagca atgtgctac acttacata actttcaca attatctcg atgctttgc cttgctgct actttcttc atatacttc ttcttgtca catatagaa gatgcccac agaatcctg tttactgct tatgttttt gatatggct atggttttt atttccttg actgtagtt caagtggga catgtggaa ggcagtgga tgttcagtt tgtggtttt attttccag ttcttcatg gtttcgtct tttgtaatg ttggccatt ttatgtgta gacatttgc atcattatt aagtacatt gacaacaat attaaacca cgaaaacgg aaagtttgg tcacttggt gttggcttt ttggttccg ttatttttc ttaatcatc tcattaatt cgaaatctc acaccagat gtaccaaac tcgtttttc aaacctcaa tatcaaact aatgcctgc tactttgaa gctgaagac caaagagtt tcaattttt tatgttcct attattttc tcaatgatc gtaagtggc atttccctg ggtgtggca aaatacata atcgtgcga tacgaccga ttaagatta aacgatttc ttttggatc gaaaacgga acgaaatac aaattcatg tttaggcaa tgctttatt acatcagtg attatgact atttcttgg atctcgaat gttttaatg agtttctta gatgagtct agtggcatc cttaaagct gccgtcttt ttcgaagct ttacagggt gttctaatt gttgctgtt ttcactatg catcattac ggtaccgct tatttggag acacgacgg caaagaaca gaaccacct gtcgaaatg aacccttta agccaaaat aatcacgaa tag
- >TC031683 RA
taagctaaa cacaaattg gatgaaact ccatctcga aacatatgg atgtgacag aattagctt gaaaacatt acgcagtta ggtcacgta aaaagttgt ctttttgca caacactaa tccaacaag aatgtaaac tatcttcag tgtaaataa ttgcacgtt ccagtctgc tacttaacg tcgataaac gggttgatt attgatgca gaaatgatc ctgtaaacg cgatcactt tataattgg acagccggc aacgatgca ttcgattcg gtacatcac ccattcaga acaccccca tgttttgac gcttagccc taattatcc cattcctgt tcgattcaa ctcccctaa tttatacga attccaatc gtaaaaatc gtcgacatt gcagaaatg ttatgtaaa tgtcgtcgt taatatgaa agaatgttg tacaggaat ttatttaaa agagatgtt tatttccac attctgtac cttcccctg atgatgatg acgctgatg aaaaccacc gctgcattg aggcgccac atacgcaac aaagaggtc gcgtcggat tgctacgtt cgactgttt ttcgggctt tctttacaa tagtccata gtgttataa ataaaataa tatgaaccg tgcatttca ataagtgtt cgtcatcta aaattagtg ccgtagtga ttgtgatgg ccagaagtg atatatgtt aatgtaata aatgattgt gttttaatt tcgacacta gaatgtatc gagtaattc tacttttgt gggcacatg ttattgtgc aaggatcta gcgaggtta tgcttgaac cgaacatga cgacattta tgcgaacag tggacggtt tttctaatc agttattag gtgtgactt ccggaaaaa tcgtgataa ccggaaaca acactcttc acctaacca atttctcct tcgatggta aagtgtccg acgcccatt ttatagtcg gcaaaggca cagtttccg aaaacggaa ctttcgtac ctttctctg aaagttccg acaatcttc cgccattag acaacgaag acatcgact taactctgc ctaataatc taacagtca ttgatatcg attggttgg caatttttt ctccaacga cccaggaaa acctaggct ttgtattgc taacaaaaa ctgtacagt catcacctc aaactttca gcttcgaca atgatgtta tttccggtc ctgtattat tactcgata aaaagacac tctttatcc cgaattttc gctacaaag gcgatcaca aaaatgtgc gcttttggg tgggcaatg gcagcgaac cgtcctcgg acggaatcg ctctgtcag acgagaaaa actcttcgg aaagtcttg ggccatatc agggacaaa atgtgaagc ttcatttac cagaaagtc tgtcaactg acaatatcg actatttgg ctgtttggt caatcgacg aggcagtta gtctaggct atgttccca tcaaaggag ttagcgaag cgccagaag ttgaacagg aaacaaaaa gattaagtg aagggaact tcgaagtga tagtaccaa agtgttgct cctcttcag ctgtaatga ccagtcgcg gatgtgagg aaaagggtc gcttacaaa tatttgtac caaacttta acatctacg aacacaatg caacacatt tcaggaatg aatctctgg acctaaaca aggtggatt ttaaaatat acgattcca caatgaagt gtcctgctg gaaaatttc ctttagacc agtcaagtg aagaattcg ctctactga ttaatggct ctttactta ttaaggggg cagccggga aaggcatat ggtcacagg aaaactttt gtttagaca acgtcgtag atgagagta atgtaacac aaatgagaa tttttgtat gcgtcccgg aagtccaaa tacagaaag caatgtgct acacttaca taactttca caattatct cgatgcttt gccttgctg ctactttct tcatatact tcttcttgt cacatatag aagatgccc acagaatcc tgtttactg cttatgttt ttgatatgg ctatggttt ttatttcct tgactgtag ttcaagtgg gacatgtgg aaggcagtg gatgttcag tttgtggtt ttattttcc agttcttca tggtttcgt cttttgtaa tgttggcca ttttatgtg tagacattt gcatcatta ttaagtaca ttgacaaca atattaaac cacgaaaac ggaaagttt ggtcacttg gtgttggct ttttggttc cgttatttt tcttaatca tctcattaa ttcgaaatc tcacaccag atgtaccaa actcgtttt tcaaacctc aatatcaaa ctaatgcct gctactttg aagctgaag accaaagag tttcaattt tttatgttc ctattattt tctcaatga tcgtaagtg gcatttccc tgggtgtgg caaaataca taatcgtgc gatacgacc gattaagat taaacgatt tcttttgga tcgaaaacg gaacgaaat acaaattca tgtggcgaa aacatttat tggttctta ttataacgt caacgttac ggatttttg atctagtgc tttatttaa gttatggac atgatagta tgttattag tagttgata tatttgagg cgactcaag gaattttta ttctattac tattcgttc tgaatgaac aaacaagaa aagagattc gaaaaagta tcgaaaaaa ggagaagaa gaagacgga agcatcatc taaaccatt caaaagaag aggaacttg tgatgtcaa agccgacct gagtgcttg aaatcatgg acatttttt ttaacgtaa tttttgctc tgacccgaa tggtcaatt agttgctaa caagttaag atggatgct tatatttat tttttgatt atagtttac atgttcaga tatcgatag tatatcttt tttatcgga ttctgtcag aaaaatagt ctgataaat aagtagggc ataaccata taatttgct gtaaatatg
- >TC031683 RB
taagctaaa cacaaattg gatgaaact ccatctcga aacatatgg atgtgacag aattagctt gaaaacatt acgcagtta ggtcacgta aaaagttgt ctttttgca caacactaa tccaacaag aatgtaaac tatcttcag tgtaaataa ttgcacgtt ccagtctgc tacttaacg tcgataaac gggttgatt attgatgca gaaatgatc ctgtaaacg cgatcactt tataattgg acagccggc aacgatgca ttcgattcg gtacatcac ccattcaga acaccccca tgttttgac gcttagccc taattatcc cattcctgt tcgattcaa ctcccctaa tttatacga attccaatc gtaaaaatc gtcgacatt gcagaaatg ttatgtaaa tgtcgtcgt taatatgaa agaatgttg tacaggaat ttatttaaa agagatgtt tatttccac attctgtac cttcccctg atgatgatg acgctgatg aaaaccacc gctgcattg aggcgccac atacgcaac aaagaggtc gcgtcggat tgctacgtt cgactgttt ttcgggctt tctttacaa tagtccata gtgttataa ataaaataa tatgaaccg tgcatttca ataagtgtt cgtcatcta aaattagtg ccgtagtga ttgtgatgg ccagaagtg atatatgtt aatgtaata aatgattgt gttttaatt tcgacacta gaatgtatc gagtaattc tacttttgt gggcacatg ttattgtgc aaggatcta gcgaggtta tgcttgaac cgaacatga cgacattta tgcgaacag tggacggtt tttctaatc agttattag gtgtgactt ccggaaaaa tcgtgataa ccggaaaca acactcttc acctaacca atttctcct tcgatggta aagtgtccg acgcccatt ttatagtcg gcaaaggca cagtttccg aaaacggaa ctttcgtac ctttctctg aaagttccg acaatcttc cgccattag acaacgaag acatcgact taactctgc ctaataatc taacagtca ttgatatcg attggttgg caatttttt ctccaacga cccaggaaa acctaggct ttgtattgc taacaaaaa ctgtacagt catcacctc aaactttca gcttcgaca atgatgtta tttccggtc ctgtattat tactcgata aaaagacac tctttatcc cgaattttc gctacaaag gcgatcaca aaaatgtgc gcttttggg tgggcaatg gcagcgaac cgtcctcgg acggaatcg ctctgtcag acgagaaaa actcttcgg aaagtcttg ggccatatc agggacaaa atgtgaagc ttcatttac cagaaagtc tgtcaactg acaatatcg actatttgg ctgtttggt caatcgacg aggcagtta gtctaggct atgttccca tcaaaggag ttagcgaag cgccagaag ttgaacagg aaacaaaaa gattaagtg aagggaact tcgaagtga tagtaccaa agtgttgct cctcttcag ctgtaatga ccagtcgcg gatgtgagg aaaagggtc gcttacaaa tatttgtac caaacttta acatctacg aacacaatg caacacatt tcaggaatg aatctctgg acctaaaca aggtggatt ttaaaatat acgattcca caatgaagt gtcctgctg gaaaatttc ctttagacc agtcaagtg aagaattcg ctctactga ttaatggct ctttactta ttaaggggg cagccggga aaggcatat ggtcacagg aaaactttt gtttagaca acgtcgtag atgagagta atgtaacac aaatgagaa tttttgtat gcgtcccgg aagtccaaa tacagaaag caatgtgct acacttaca taactttca caattatct cgatgcttt gccttgctg ctactttct tcatatact tcttcttgt cacatatag aagatgccc acagaatcc tgtttactg cttatgttt ttgatatgg ctatggttt ttatttcct tgactgtag ttcaagtgg gacatgtgg aaggcagtg gatgttcag tttgtggtt ttattttcc agttcttca tggtttcgt cttttgtaa tgttggcca ttttatgtg tagacattt gcatcatta ttaagtaca ttgacaaca atattaaac cacgaaaac ggaaagttt ggtcacttg gtgttggct ttttggttc cgttatttt tcttaatca tctcattaa ttcgaaatc tcacaccag atgtaccaa actcgtttt tcaaacctc aatatcaaa ctaatgcct gctactttg aagctgaag accaaagag tttcaattt tttatgttc ctattattt tctcaatga tcgtaagtg gcatttccc tgggtgtgg caaaataca taatcgtgc gatacgacc gattaagat taaacgatt tcttttgga tcgaaaacg gaacgaaat acaaattca tgtttaggc aatgcttta ttacatcag tgattatga ctatttctt ggatctcga atgttttaa tgagtttct tagatgagt ctagtggca tccttaaag ctgccgtct ttttcgaag ctttacagg gtgttctaa ttgttgctg ttttcacta tgcatcatt acggtaccg cttatttgg agacacgac ggcaaagaa cagaaccac ctgtcgaaa tgaaccctt taagccaaa ataatcacg aatagggta aatattact tgtgttcgt gttatcacc aatacatca aaaatcaaa cgacaggca ctttatcgt gtgcaaaaa aatcaataa tgactagag tttatcctg atataaaaa tgggttgaa ccgagttgc ctaaactcg atgacaaat cataaacta aatacttca ttccagtct cttcctacc tctatttac taacttgca caacactga acactcatt tatctctat ttttgtgat tgcactaac tttcgaatt ttttaggtg gcgaaaaca tttattggt tcttattat aacgtcaac gttacggat ttttgatct agtgcttta tttaagtta tggacatga tagtatgtt attagtagt tgatatatt tgaggcgac tcaaggaat ttttattct attactatt cgttctgaa tgaacaaac aagaaaaga gattcgaaa aagtatcga aaaaaggag aagaagaag acggaagca tcatctaaa ccattcaaa agaagagga acttgtgat gtcaaagcc gacctgagt gcttgaaat catggacat tttttttaa cgtaatttt tgctctgac ccgaatggt caattagtt gctaacaag ttaagatgg atgcttata tttattttt tgattatag tttacatgt tcagatatc gatagtata tctttttta tcggattct gtcagaaaa atagtctga taaataagt agggcataa ccatataat ttgctgtaa atatg
