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Gene information
General information
TC031683 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 412454..442797
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031683
    iBeetle screen iB_06089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031683 RA
      MYRVILLLW AHVIVQGSS EVMLEPNMT TFMRTVDGF 
      SNQLLGVTS GKIVITGNN TLHLTNFSF DGKVSDAHF 
      IVGKGTVSE NGTFVPFSE SSDNLPPLD NEDIDLTLP 
      NNLTVIDID WLAIFSPTT QENLGFVLL TKTVQSSPQ 
      TFSFDNDVI SGPVLLLDK KTLFIPNFR YKGDHKNVR 
      FWVGNGSEP SSDGIALSD EKNSSESLG PYQGQNVKL 
      HLPESLSTD NIDYLAVWS IDEAVSLGY VPIKGVSEA 
      PEVEQETKR LSEGNFEVI VPKCCSSSA VMTSRGCEE 
      KGRLQIFVP NFNIYEHNA THFRNESLD LNKVDFKIY 
      DSTMKCPAG KFPLDQSSE EFALLINGS LLIKGAAGK 
      GIWSQENFC LDNVVDESN VTQMRIFVC VPEVQIQKA 
      MCYTYITFT IISMLCLAA TFFIYFFLS HIEDAHRIL 
      FTAYVFDMA MVFISLTVV QVGHVEGSG CSVCGFIFQ 
      FFMVSSFVM LAILCVDIC IIIKYIDNN IKPRKRKVW 
      SLGVGFLVP LFFLIISLI RNLTPDVPN SFFKPQYQT 
      NACYFEAED QRVSIFYVP IIFSMIVSG ISLGVAKYI 
      IVRYDRLRL NDFFWIENG TKYKFMWRK HLLVLIITS 
      TLRIFDLVL YLSYGHDSM LLVVDIFEA TQGIFILLL 
      FVLNEQTRK EIRKSIEKR RRRRRKHHL NHSKEEELV 
      MSKPT
    • >TC031683 RB
      MYRVILLLW AHVIVQGSS EVMLEPNMT TFMRTVDGF 
      SNQLLGVTS GKIVITGNN TLHLTNFSF DGKVSDAHF 
      IVGKGTVSE NGTFVPFSE SSDNLPPLD NEDIDLTLP 
      NNLTVIDID WLAIFSPTT QENLGFVLL TKTVQSSPQ 
      TFSFDNDVI SGPVLLLDK KTLFIPNFR YKGDHKNVR 
      FWVGNGSEP SSDGIALSD EKNSSESLG PYQGQNVKL 
      HLPESLSTD NIDYLAVWS IDEAVSLGY VPIKGVSEA 
      PEVEQETKR LSEGNFEVI VPKCCSSSA VMTSRGCEE 
      KGRLQIFVP NFNIYEHNA THFRNESLD LNKVDFKIY 
      DSTMKCPAG KFPLDQSSE EFALLINGS LLIKGAAGK 
      GIWSQENFC LDNVVDESN VTQMRIFVC VPEVQIQKA 
      MCYTYITFT IISMLCLAA TFFIYFFLS HIEDAHRIL 
      FTAYVFDMA MVFISLTVV QVGHVEGSG CSVCGFIFQ 
      FFMVSSFVM LAILCVDIC IIIKYIDNN IKPRKRKVW 
      SLGVGFLVP LFFLIISLI RNLTPDVPN SFFKPQYQT 
      NACYFEAED QRVSIFYVP IIFSMIVSG ISLGVAKYI 
      IVRYDRLRL NDFFWIENG TKYKFMFRQ CFITSVIMT 
      ISWISNVLM SFLDESSGI LKAAVFFEA LQGVLIVAV 
      FTMHHYGTA YLETRRQRT EPPVEMNPL SQNNHE
    CDS sequence
    • >TC031683 RA
      atgtatcga gtaattcta cttttgtgg gcacatgtt 
      attgtgcaa ggatctagc gaggttatg cttgaaccg 
      aacatgacg acatttatg cgaacagtg gacggtttt 
      tctaatcag ttattaggt gtgacttcc ggaaaaatc 
      gtgataacc ggaaacaac actcttcac ctaaccaat 
      ttctccttc gatggtaaa gtgtccgac gcccatttt 
      atagtcggc aaaggcaca gtttccgaa aacggaact 
      ttcgtacct ttctctgaa agttccgac aatcttccg 
      ccattagac aacgaagac atcgactta actctgcct 
      aataatcta acagtcatt gatatcgat tggttggca 
      attttttct ccaacgacc caggaaaac ctaggcttt 
      gtattgcta acaaaaact gtacagtca tcacctcaa 
      actttcagc ttcgacaat gatgttatt tccggtcct 
      gtattatta ctcgataaa aagacactc tttatcccg 
      aattttcgc tacaaaggc gatcacaaa aatgtgcgc 
      ttttgggtg ggcaatggc agcgaaccg tcctcggac 
      ggaatcgct ctgtcagac gagaaaaac tcttcggaa 
      agtcttggg ccatatcag ggacaaaat gtgaagctt 
      catttacca gaaagtctg tcaactgac aatatcgac 
      tatttggct gtttggtca atcgacgag gcagttagt 
      ctaggctat gttcccatc aaaggagtt agcgaagcg 
      ccagaagtt gaacaggaa acaaaaaga ttaagtgaa 
      gggaacttc gaagtgata gtaccaaag tgttgctcc 
      tcttcagct gtaatgacc agtcgcgga tgtgaggaa 
      aagggtcgc ttacaaata tttgtacca aactttaac 
      atctacgaa cacaatgca acacatttc aggaatgaa 
      tctctggac ctaaacaag gtggatttt aaaatatac 
      gattccaca atgaagtgt cctgctgga aaatttcct 
      ttagaccag tcaagtgaa gaattcgct ctactgatt 
      aatggctct ttacttatt aagggggca gccgggaaa 
      ggcatatgg tcacaggaa aacttttgt ttagacaac 
      gtcgtagat gagagtaat gtaacacaa atgagaatt 
      tttgtatgc gtcccggaa gtccaaata cagaaagca 
      atgtgctac acttacata actttcaca attatctcg 
      atgctttgc cttgctgct actttcttc atatacttc 
      ttcttgtca catatagaa gatgcccac agaatcctg 
      tttactgct tatgttttt gatatggct atggttttt 
      atttccttg actgtagtt caagtggga catgtggaa 
      ggcagtgga tgttcagtt tgtggtttt attttccag 
      ttcttcatg gtttcgtct tttgtaatg ttggccatt 
      ttatgtgta gacatttgc atcattatt aagtacatt 
      gacaacaat attaaacca cgaaaacgg aaagtttgg 
      tcacttggt gttggcttt ttggttccg ttatttttc 
      ttaatcatc tcattaatt cgaaatctc acaccagat 
      gtaccaaac tcgtttttc aaacctcaa tatcaaact 
      aatgcctgc tactttgaa gctgaagac caaagagtt 
      tcaattttt tatgttcct attattttc tcaatgatc 
      gtaagtggc atttccctg ggtgtggca aaatacata 
      atcgtgcga tacgaccga ttaagatta aacgatttc 
      ttttggatc gaaaacgga acgaaatac aaattcatg 
      tggcgaaaa catttattg gttcttatt ataacgtca 
      acgttacgg atttttgat ctagtgctt tatttaagt 
      tatggacat gatagtatg ttattagta gttgatata 
      tttgaggcg actcaagga atttttatt ctattacta 
      ttcgttctg aatgaacaa acaagaaaa gagattcga 
      aaaagtatc gaaaaaagg agaagaaga agacggaag 
      catcatcta aaccattca aaagaagag gaacttgtg 
      atgtcaaag ccgacctga
    • >TC031683 RB
      atgtatcga gtaattcta cttttgtgg gcacatgtt 
      attgtgcaa ggatctagc gaggttatg cttgaaccg 
      aacatgacg acatttatg cgaacagtg gacggtttt 
      tctaatcag ttattaggt gtgacttcc ggaaaaatc 
      gtgataacc ggaaacaac actcttcac ctaaccaat 
      ttctccttc gatggtaaa gtgtccgac gcccatttt 
      atagtcggc aaaggcaca gtttccgaa aacggaact 
      ttcgtacct ttctctgaa agttccgac aatcttccg 
      ccattagac aacgaagac atcgactta actctgcct 
      aataatcta acagtcatt gatatcgat tggttggca 
      attttttct ccaacgacc caggaaaac ctaggcttt 
      gtattgcta acaaaaact gtacagtca tcacctcaa 
      actttcagc ttcgacaat gatgttatt tccggtcct 
      gtattatta ctcgataaa aagacactc tttatcccg 
      aattttcgc tacaaaggc gatcacaaa aatgtgcgc 
      ttttgggtg ggcaatggc agcgaaccg tcctcggac 
      ggaatcgct ctgtcagac gagaaaaac tcttcggaa 
      agtcttggg ccatatcag ggacaaaat gtgaagctt 
      catttacca gaaagtctg tcaactgac aatatcgac 
      tatttggct gtttggtca atcgacgag gcagttagt 
      ctaggctat gttcccatc aaaggagtt agcgaagcg 
      ccagaagtt gaacaggaa acaaaaaga ttaagtgaa 
      gggaacttc gaagtgata gtaccaaag tgttgctcc 
      tcttcagct gtaatgacc agtcgcgga tgtgaggaa 
      aagggtcgc ttacaaata tttgtacca aactttaac 
      atctacgaa cacaatgca acacatttc aggaatgaa 
      tctctggac ctaaacaag gtggatttt aaaatatac 
      gattccaca atgaagtgt cctgctgga aaatttcct 
      ttagaccag tcaagtgaa gaattcgct ctactgatt 
      aatggctct ttacttatt aagggggca gccgggaaa 
      ggcatatgg tcacaggaa aacttttgt ttagacaac 
      gtcgtagat gagagtaat gtaacacaa atgagaatt 
      tttgtatgc gtcccggaa gtccaaata cagaaagca 
      atgtgctac acttacata actttcaca attatctcg 
      atgctttgc cttgctgct actttcttc atatacttc 
      ttcttgtca catatagaa gatgcccac agaatcctg 
      tttactgct tatgttttt gatatggct atggttttt 
      atttccttg actgtagtt caagtggga catgtggaa 
      ggcagtgga tgttcagtt tgtggtttt attttccag 
      ttcttcatg gtttcgtct tttgtaatg ttggccatt 
      ttatgtgta gacatttgc atcattatt aagtacatt 
      gacaacaat attaaacca cgaaaacgg aaagtttgg 
      tcacttggt gttggcttt ttggttccg ttatttttc 
      ttaatcatc tcattaatt cgaaatctc acaccagat 
      gtaccaaac tcgtttttc aaacctcaa tatcaaact 
      aatgcctgc tactttgaa gctgaagac caaagagtt 
      tcaattttt tatgttcct attattttc tcaatgatc 
      gtaagtggc atttccctg ggtgtggca aaatacata 
      atcgtgcga tacgaccga ttaagatta aacgatttc 
      ttttggatc gaaaacgga acgaaatac aaattcatg 
      tttaggcaa tgctttatt acatcagtg attatgact 
      atttcttgg atctcgaat gttttaatg agtttctta 
      gatgagtct agtggcatc cttaaagct gccgtcttt 
      ttcgaagct ttacagggt gttctaatt gttgctgtt 
      ttcactatg catcattac ggtaccgct tatttggag 
      acacgacgg caaagaaca gaaccacct gtcgaaatg 
      aacccttta agccaaaat aatcacgaa tag
    mRNA sequence
    • >TC031683 RA
      taagctaaa cacaaattg gatgaaact ccatctcga 
      aacatatgg atgtgacag aattagctt gaaaacatt 
      acgcagtta ggtcacgta aaaagttgt ctttttgca 
      caacactaa tccaacaag aatgtaaac tatcttcag 
      tgtaaataa ttgcacgtt ccagtctgc tacttaacg 
      tcgataaac gggttgatt attgatgca gaaatgatc 
      ctgtaaacg cgatcactt tataattgg acagccggc 
      aacgatgca ttcgattcg gtacatcac ccattcaga 
      acaccccca tgttttgac gcttagccc taattatcc 
      cattcctgt tcgattcaa ctcccctaa tttatacga 
      attccaatc gtaaaaatc gtcgacatt gcagaaatg 
      ttatgtaaa tgtcgtcgt taatatgaa agaatgttg 
      tacaggaat ttatttaaa agagatgtt tatttccac 
      attctgtac cttcccctg atgatgatg acgctgatg 
      aaaaccacc gctgcattg aggcgccac atacgcaac 
      aaagaggtc gcgtcggat tgctacgtt cgactgttt 
      ttcgggctt tctttacaa tagtccata gtgttataa 
      ataaaataa tatgaaccg tgcatttca ataagtgtt 
      cgtcatcta aaattagtg ccgtagtga ttgtgatgg 
      ccagaagtg atatatgtt aatgtaata aatgattgt 
      gttttaatt tcgacacta gaatgtatc gagtaattc 
      tacttttgt gggcacatg ttattgtgc aaggatcta 
      gcgaggtta tgcttgaac cgaacatga cgacattta 
      tgcgaacag tggacggtt tttctaatc agttattag 
      gtgtgactt ccggaaaaa tcgtgataa ccggaaaca 
      acactcttc acctaacca atttctcct tcgatggta 
      aagtgtccg acgcccatt ttatagtcg gcaaaggca 
      cagtttccg aaaacggaa ctttcgtac ctttctctg 
      aaagttccg acaatcttc cgccattag acaacgaag 
      acatcgact taactctgc ctaataatc taacagtca 
      ttgatatcg attggttgg caatttttt ctccaacga 
      cccaggaaa acctaggct ttgtattgc taacaaaaa 
      ctgtacagt catcacctc aaactttca gcttcgaca 
      atgatgtta tttccggtc ctgtattat tactcgata 
      aaaagacac tctttatcc cgaattttc gctacaaag 
      gcgatcaca aaaatgtgc gcttttggg tgggcaatg 
      gcagcgaac cgtcctcgg acggaatcg ctctgtcag 
      acgagaaaa actcttcgg aaagtcttg ggccatatc 
      agggacaaa atgtgaagc ttcatttac cagaaagtc 
      tgtcaactg acaatatcg actatttgg ctgtttggt 
      caatcgacg aggcagtta gtctaggct atgttccca 
      tcaaaggag ttagcgaag cgccagaag ttgaacagg 
      aaacaaaaa gattaagtg aagggaact tcgaagtga 
      tagtaccaa agtgttgct cctcttcag ctgtaatga 
      ccagtcgcg gatgtgagg aaaagggtc gcttacaaa 
      tatttgtac caaacttta acatctacg aacacaatg 
      caacacatt tcaggaatg aatctctgg acctaaaca 
      aggtggatt ttaaaatat acgattcca caatgaagt 
      gtcctgctg gaaaatttc ctttagacc agtcaagtg 
      aagaattcg ctctactga ttaatggct ctttactta 
      ttaaggggg cagccggga aaggcatat ggtcacagg 
      aaaactttt gtttagaca acgtcgtag atgagagta 
      atgtaacac aaatgagaa tttttgtat gcgtcccgg 
      aagtccaaa tacagaaag caatgtgct acacttaca 
      taactttca caattatct cgatgcttt gccttgctg 
      ctactttct tcatatact tcttcttgt cacatatag 
      aagatgccc acagaatcc tgtttactg cttatgttt 
      ttgatatgg ctatggttt ttatttcct tgactgtag 
      ttcaagtgg gacatgtgg aaggcagtg gatgttcag 
      tttgtggtt ttattttcc agttcttca tggtttcgt 
      cttttgtaa tgttggcca ttttatgtg tagacattt 
      gcatcatta ttaagtaca ttgacaaca atattaaac 
      cacgaaaac ggaaagttt ggtcacttg gtgttggct 
      ttttggttc cgttatttt tcttaatca tctcattaa 
      ttcgaaatc tcacaccag atgtaccaa actcgtttt 
      tcaaacctc aatatcaaa ctaatgcct gctactttg 
      aagctgaag accaaagag tttcaattt tttatgttc 
      ctattattt tctcaatga tcgtaagtg gcatttccc 
      tgggtgtgg caaaataca taatcgtgc gatacgacc 
      gattaagat taaacgatt tcttttgga tcgaaaacg 
      gaacgaaat acaaattca tgtggcgaa aacatttat 
      tggttctta ttataacgt caacgttac ggatttttg 
      atctagtgc tttatttaa gttatggac atgatagta 
      tgttattag tagttgata tatttgagg cgactcaag 
      gaattttta ttctattac tattcgttc tgaatgaac 
      aaacaagaa aagagattc gaaaaagta tcgaaaaaa 
      ggagaagaa gaagacgga agcatcatc taaaccatt 
      caaaagaag aggaacttg tgatgtcaa agccgacct 
      gagtgcttg aaatcatgg acatttttt ttaacgtaa 
      tttttgctc tgacccgaa tggtcaatt agttgctaa 
      caagttaag atggatgct tatatttat tttttgatt 
      atagtttac atgttcaga tatcgatag tatatcttt 
      tttatcgga ttctgtcag aaaaatagt ctgataaat 
      aagtagggc ataaccata taatttgct gtaaatatg
    • >TC031683 RB
      taagctaaa cacaaattg gatgaaact ccatctcga 
      aacatatgg atgtgacag aattagctt gaaaacatt 
      acgcagtta ggtcacgta aaaagttgt ctttttgca 
      caacactaa tccaacaag aatgtaaac tatcttcag 
      tgtaaataa ttgcacgtt ccagtctgc tacttaacg 
      tcgataaac gggttgatt attgatgca gaaatgatc 
      ctgtaaacg cgatcactt tataattgg acagccggc 
      aacgatgca ttcgattcg gtacatcac ccattcaga 
      acaccccca tgttttgac gcttagccc taattatcc 
      cattcctgt tcgattcaa ctcccctaa tttatacga 
      attccaatc gtaaaaatc gtcgacatt gcagaaatg 
      ttatgtaaa tgtcgtcgt taatatgaa agaatgttg 
      tacaggaat ttatttaaa agagatgtt tatttccac 
      attctgtac cttcccctg atgatgatg acgctgatg 
      aaaaccacc gctgcattg aggcgccac atacgcaac 
      aaagaggtc gcgtcggat tgctacgtt cgactgttt 
      ttcgggctt tctttacaa tagtccata gtgttataa 
      ataaaataa tatgaaccg tgcatttca ataagtgtt 
      cgtcatcta aaattagtg ccgtagtga ttgtgatgg 
      ccagaagtg atatatgtt aatgtaata aatgattgt 
      gttttaatt tcgacacta gaatgtatc gagtaattc 
      tacttttgt gggcacatg ttattgtgc aaggatcta 
      gcgaggtta tgcttgaac cgaacatga cgacattta 
      tgcgaacag tggacggtt tttctaatc agttattag 
      gtgtgactt ccggaaaaa tcgtgataa ccggaaaca 
      acactcttc acctaacca atttctcct tcgatggta 
      aagtgtccg acgcccatt ttatagtcg gcaaaggca 
      cagtttccg aaaacggaa ctttcgtac ctttctctg 
      aaagttccg acaatcttc cgccattag acaacgaag 
      acatcgact taactctgc ctaataatc taacagtca 
      ttgatatcg attggttgg caatttttt ctccaacga 
      cccaggaaa acctaggct ttgtattgc taacaaaaa 
      ctgtacagt catcacctc aaactttca gcttcgaca 
      atgatgtta tttccggtc ctgtattat tactcgata 
      aaaagacac tctttatcc cgaattttc gctacaaag 
      gcgatcaca aaaatgtgc gcttttggg tgggcaatg 
      gcagcgaac cgtcctcgg acggaatcg ctctgtcag 
      acgagaaaa actcttcgg aaagtcttg ggccatatc 
      agggacaaa atgtgaagc ttcatttac cagaaagtc 
      tgtcaactg acaatatcg actatttgg ctgtttggt 
      caatcgacg aggcagtta gtctaggct atgttccca 
      tcaaaggag ttagcgaag cgccagaag ttgaacagg 
      aaacaaaaa gattaagtg aagggaact tcgaagtga 
      tagtaccaa agtgttgct cctcttcag ctgtaatga 
      ccagtcgcg gatgtgagg aaaagggtc gcttacaaa 
      tatttgtac caaacttta acatctacg aacacaatg 
      caacacatt tcaggaatg aatctctgg acctaaaca 
      aggtggatt ttaaaatat acgattcca caatgaagt 
      gtcctgctg gaaaatttc ctttagacc agtcaagtg 
      aagaattcg ctctactga ttaatggct ctttactta 
      ttaaggggg cagccggga aaggcatat ggtcacagg 
      aaaactttt gtttagaca acgtcgtag atgagagta 
      atgtaacac aaatgagaa tttttgtat gcgtcccgg 
      aagtccaaa tacagaaag caatgtgct acacttaca 
      taactttca caattatct cgatgcttt gccttgctg 
      ctactttct tcatatact tcttcttgt cacatatag 
      aagatgccc acagaatcc tgtttactg cttatgttt 
      ttgatatgg ctatggttt ttatttcct tgactgtag 
      ttcaagtgg gacatgtgg aaggcagtg gatgttcag 
      tttgtggtt ttattttcc agttcttca tggtttcgt 
      cttttgtaa tgttggcca ttttatgtg tagacattt 
      gcatcatta ttaagtaca ttgacaaca atattaaac 
      cacgaaaac ggaaagttt ggtcacttg gtgttggct 
      ttttggttc cgttatttt tcttaatca tctcattaa 
      ttcgaaatc tcacaccag atgtaccaa actcgtttt 
      tcaaacctc aatatcaaa ctaatgcct gctactttg 
      aagctgaag accaaagag tttcaattt tttatgttc 
      ctattattt tctcaatga tcgtaagtg gcatttccc 
      tgggtgtgg caaaataca taatcgtgc gatacgacc 
      gattaagat taaacgatt tcttttgga tcgaaaacg 
      gaacgaaat acaaattca tgtttaggc aatgcttta 
      ttacatcag tgattatga ctatttctt ggatctcga 
      atgttttaa tgagtttct tagatgagt ctagtggca 
      tccttaaag ctgccgtct ttttcgaag ctttacagg 
      gtgttctaa ttgttgctg ttttcacta tgcatcatt 
      acggtaccg cttatttgg agacacgac ggcaaagaa 
      cagaaccac ctgtcgaaa tgaaccctt taagccaaa 
      ataatcacg aatagggta aatattact tgtgttcgt 
      gttatcacc aatacatca aaaatcaaa cgacaggca 
      ctttatcgt gtgcaaaaa aatcaataa tgactagag 
      tttatcctg atataaaaa tgggttgaa ccgagttgc 
      ctaaactcg atgacaaat cataaacta aatacttca 
      ttccagtct cttcctacc tctatttac taacttgca 
      caacactga acactcatt tatctctat ttttgtgat 
      tgcactaac tttcgaatt ttttaggtg gcgaaaaca 
      tttattggt tcttattat aacgtcaac gttacggat 
      ttttgatct agtgcttta tttaagtta tggacatga 
      tagtatgtt attagtagt tgatatatt tgaggcgac 
      tcaaggaat ttttattct attactatt cgttctgaa 
      tgaacaaac aagaaaaga gattcgaaa aagtatcga 
      aaaaaggag aagaagaag acggaagca tcatctaaa 
      ccattcaaa agaagagga acttgtgat gtcaaagcc 
      gacctgagt gcttgaaat catggacat tttttttaa 
      cgtaatttt tgctctgac ccgaatggt caattagtt 
      gctaacaag ttaagatgg atgcttata tttattttt 
      tgattatag tttacatgt tcagatatc gatagtata 
      tctttttta tcggattct gtcagaaaa atagtctga 
      taaataagt agggcataa ccatataat ttgctgtaa 
      atatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY