Status
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Gene information
General information
TC031677
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002274
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 269321..277932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031677
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06088

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031677 RA
      MGELCSWSF QDGHRTNIT MAYSPTSPL LEKVFENLC 
      LNVTPPPNL ILKSYRTAA EMNADIPDN RFNFALQLD 
      DSLFGLDDS SKLPENVDV TLRFPSEDS IVSRREWYT 
      NLIYPLLQT PGPRSSQDN WGGEPGYVN RGFLYAQAI 
      ITKIFINNG ENIPLNTEV VMQRFPYPE WLNDAFYLD 
      QMETMVSLM IMMSFMFNY VNAIRAITT EKEKQLKES 
      MKVMGLPGW LHWMAWFLR SFVILLIAI ILITIVLKV 
      NFQKNAVFV HSDGTVLFV FFILFACST ITFTFLISV 
      FFSKANTAT AVGSLVFFL TYLPYTLQQ QQGDIVSLG 
      SALSSSLLA NSGLSFAIS VILKFEAIE EGSQWSNLF 
      STVSPDDNL TLGAFFLMF ILDTFLYLC IALYIEAVF 
      PGDFGVPQP WYFPFTRAY WCNNIQAMG DEQSSENKG 
      EFYEDFTEK LPVGIQLKN LSKTFGGNK AVINMNLDM 
      YEGHITVLL GHNGAGKTT TMSMITGMF PPTSGTAIV 
      SGYDVRTSI QNVRDSMGL CLQHNVLFD NLTVWEHLY 
      FFGKLKGLR NDEINEEID RYLKLLELE DKRDAHSST 
      LSGGMKRKL SVGMALCGK SKVVMLDEP TAGMDPSAR 
      RAIWNLLQK QKSGRTILL TTHYMDEAD LLGDRIAIM 
      TGGELQCCG SSFFLKKKY GAGYYLIMD VTPNCQPSK 
      ITQLLQNYI PNVQIHSHV GSELTYQLP ENESWKFEK 
      MLGQLENES TSLGVQSYG VSLTTLEEV FMKVGADHD 
      NKLSKKING NSESTITVE ESGLSSLNI AHLSGFNLL 
      RNQFIAIVL KKNLSLLRS WYLFLLQVI LPVALLIIT 
      VLNARGYAP KTSFPALKM SLESYNEPI TLVQNDGSS 
      LANTYINML NGYPAKTVN NLESEIINL TKESPSTVK 
      RHYTVAASF NSTVATAWF NGDPYHSTS LSLSLVLNT 
      VYKSKFGEK KTLNFINHP LPLSKEAEL NNMQFNLMV 
      YQIAMELGY GMAFVASFY ILFYIRERV SKSKHLQFV 
      SGVNAFVFW GTAFLCDLI TYLITVIFV LITFAVLQE 
      DGFKTTDEI GRITLVLVY FGFFVLPFV YLTSYLFSI 
      PSSGFSRMM LLGAVSGIV GITAMQVLE IEALGVLYV 
      ARPLHWVLL FVPFYTVAK GAYDVGSIS TLRDVCLKN 
      GASYEQACA ANSMCCNID NYYSFDTPG IGRNIVISF 
      LMSFILFAV LLINEYGLF SYVMNKIIN YNKPPLQNV 
      SLESDVQEE NEKIRNTSE YDLTKTYSL VLRDVTKYY 
      KNFLAVNGL CLGVKPYEC FGLLGVNGA GKTTTFKMM 
      TGDEQISYG EAWVLGHNI KTEQKQVQK LIGYCPQFD 
      ALLDDLTVK ETLLIFGLI RGIPYKKCI PLAVNLAHE 
      FDFYKHIHK KVKELSGGN KRKLSTALA LIGDPPIIY 
      LDEPTAGMD PATKRFLWT ALAKLRDRG KCIVLTSHS 
      MEECEALCT RIAIMVNGT FQCLGSTQR LKNKFAQGY 
      ALTIKVKKH SDNVSLDDE ITVIDRFIQ THFPGAELK 
      EKYQELVSY QLVNNSSLT WSKMFGILE RGKKDLNIE 
      DYSLGQCSL EQVFLSFTK YQN
    CDS sequence
    • >TC031677 RA
      atgggagaa ttatgttct tggagtttt caagacggt 
      cataggact aatattacc atggcgtat tcgccaacc 
      agcccacta ctagaaaaa gtgttcgaa aatctttgt 
      ctcaacgtt acaccacca cccaattta attcttaaa 
      agttataga actgcagct gaaatgaac gctgatata 
      ccagataat agatttaat tttgcttta caattggac 
      gattcttta tttggactt gatgacagc agcaaactg 
      cctgaaaat gtggatgtc actttacgg tttccatca 
      gaagattca attgtttct agacgagag tggtacact 
      aatttaata tacccgctt ttacaaaca ccaggccca 
      agatcgagt caggacaac tggggtgga gaaccaggg 
      tatgtcaac cgaggattt ctctatgcc caagctatt 
      attactaaa atatttatt aataacggc gaaaatatt 
      cctttgaat acagaagtg gttatgcag cgattccct 
      taccctgaa tggttaaac gatgccttt taccttgat 
      caaatggaa acgatggta tcgctgatg ataatgatg 
      agttttatg tttaactat gttaacgct ataagggcc 
      ataacaaca gaaaaagaa aagcaactc aaggaatct 
      atgaaagtt atgggcctc cctgggtgg ttacattgg 
      atggcatgg ttcctacgt agctttgtt attctccta 
      atagcaata attctaatc acaattgtc ttaaaagta 
      aatttccaa aagaatgca gtctttgtg cactccgac 
      ggcactgtc ttatttgtg ttttttata ctttttgca 
      tgcagcacc atcacgttc acttttcta ataagcgtg 
      tttttttcg aaagcaaat actgcaaca gccgttggc 
      agtctggta ttctttctt acatatctg ccatacaca 
      ctgcaacaa cagcaaggt gatatagtg tctttaggt 
      tctgcacta agttcaagt cttttggcc aactctgga 
      ttgtctttt gccatctct gttattcta aaatttgaa 
      gcaattgaa gaaggaagt caatggagt aatttgttt 
      tcaactgta tcccccgat gataactta acacttggt 
      gcattcttc ttaatgttt atcctcgac acattcctc 
      tatttgtgt atagcttta tacatagaa gcagtgttt 
      cccggtgac tttggagtg ccacaacct tggtacttc 
      ccttttacg agagcttac tggtgtaac aatatccag 
      gctatgggt gatgaacag tcgtctgag aataaggga 
      gaattctat gaagatttc accgaaaaa ctacctgtt 
      ggtatacaa ttgaagaac ctttcaaaa acatttgga 
      ggcaataaa gcagttata aatatgaat ttggatatg 
      tatgaaggc catatcact gttttgctt ggccacaat 
      ggtgcaggc aaaaccaca acaatgtct atgataacc 
      ggaatgttt ccacctact agtggaacc gctatagtt 
      agcggatat gatgtaaga accagtata caaaatgtt 
      cgggacagt atgggtttg tgccttcaa cacaacgtc 
      ttgtttgat aacttaact gtttgggaa cacttgtat 
      ttctttggt aaattaaaa ggtttaaga aacgacgaa 
      attaatgag gaaattgat cgttacctg aagctactt 
      gagctggaa gataagaga gatgcacat tcaagtact 
      ctgtctgga ggtatgaag cgaaagtta agtgtagga 
      atggcgctt tgtggaaaa tctaaagta gtgatgctt 
      gatgaacca acagcaggg atggaccct tcagcaaga 
      agagcgatt tggaattta cttcaaaag caaaagagc 
      ggacgaaca attctacta acaacacac tacatggat 
      gaagcagat ctattagga gataggatt gctataatg 
      acaggtggc gaattacaa tgttgcgga tcaagtttc 
      ttcttaaag aagaaatac ggcgctggt tattaccta 
      atcatggat gtaacacca aattgccaa ccgtccaaa 
      attacacag ctgctgcaa aattatata ccaaacgtg 
      caaatacat tctcacgta ggttctgag ctgacttat 
      caactaccc gaaaatgaa tcatggaaa tttgaaaaa 
      atgctaggc cagttagaa aatgaaagt accagtctg 
      ggtgtgcaa agttacgga gtatcgcta acaacactt 
      gaagaagtt tttatgaaa gtcggtgcc gaccatgat 
      aataaactt tcgaaaaaa ataaatgga aacagtgaa 
      agtacaata actgtggaa gaatctggt ttaagtagt 
      ttaaacatc gcccatctg agtggtttc aacttactg 
      aggaatcag ttcatagcc attgtcctg aagaaaaac 
      ttatctctt ctaagatct tggtacttg ttcctactc 
      caagttatt ttacctgtg gcacttttg ataatcaca 
      gttctaaat gcccgaggt tacgcaccc aaaacttcg 
      tttcccgcc ttaaaaatg tctctagaa tcgtacaat 
      gaaccaata acactagtc caaaatgat ggcagttca 
      ctggccaat acatacata aatatgcta aatgggtac 
      ccagcaaaa acagttaat aatcttgaa tcggaaatc 
      ataaatttg acaaaagag tcaccatca actgtaaaa 
      aggcactat actgttgcg gcttctttc aacagtaca 
      gttgccacc gcttggttt aacggtgat ccttatcac 
      tcaacatca ctatccctc agtcttgtg ttgaatact 
      gtttacaag agcaaattt ggagaaaaa aaaaccctt 
      aattttatt aatcacccg cttcctctg agcaaggaa 
      gctgaactc aacaatatg caatttaac cttatggtg 
      taccaaata gcaatggaa ctaggatac ggaatggct 
      ttcgttgct agtttctac attttattt tatatccga 
      gaacgagtt agtaaatcc aaacacctg cagttcgtt 
      tctggtgta aatgctttc gttttctgg ggaactgct 
      ttcctttgc gatctaatc acatatctg ataacagtc 
      atttttgtt ctcatcaca tttgcagtt ctgcaggaa 
      gacggtttt aagacaacg gacgaaata ggtcgcata 
      actttagtg cttgtttat tttggcttc tttgtctta 
      ccatttgtt tatttaaca tcgtacttg ttcagtatt 
      ccatcttcc ggattttct agaatgatg ttgttggga 
      gcagtttct ggcattgtt ggaattacg gccatgcaa 
      gttttggaa attgaagct ctaggtgtt ttatatgtg 
      gcacgacct ctccattgg gtacttctt tttgttcct 
      ttctacact gtagctaaa ggagcttat gatgtgggt 
      tcaataagt actttgaga gatgtttgt ctaaaaaat 
      ggtgcaagt tatgaacaa gcttgtgcc gccaactca 
      atgtgttgc aatattgac aactactat tcttttgat 
      acacccggt attgggcgc aatatagtt atttccttt 
      cttatgtcg tttatcctc tttgctgta ctcttaatc 
      aacgaatat ggtttgttc agttacgtt atgaacaaa 
      ataattaat tacaataaa ccgccactt caaaacgta 
      tctctcgaa agtgatgtg caagaagaa aacgaaaaa 
      attcgaaat acatctgaa tacgacctc acgaaaacc 
      tactctctc gttctccga gatgtcaca aagtattac 
      aagaacttc cttgcggtt aatggcctt tgcttgggt 
      gtaaaaccc tacgaatgc ttcgggtta ttgggagtt 
      aacggtgct ggtaaaact accactttc aaaatgatg 
      accggcgat gaacaaata tcgtatggt gaggcctgg 
      gtgctagga cacaacatt aaaacggaa caaaagcaa 
      gtccaaaaa ctgatcggt tattgtccc cagtttgat 
      gccttgtta gatgacctc acggtcaaa gaaacactg 
      ctgattttt ggcctcatc cgaggaatt ccttacaag 
      aaatgcatc ccgttggcc gtaaatcta gcccatgaa 
      ttcgacttc tataaacac atccacaaa aaagtgaag 
      gaactcagc ggaggaaat aagcgaaaa ctgagcact 
      gctcttgcc ttgatcggg gatcccccg attatttat 
      ctcgatgag cccaccgca ggaatggac ccagcaaca 
      aaacggttt ctatggaca gcgctagct aagttgagg 
      gatcgagga aagtgcatc gttttgacg tcacacagt 
      atggaagag tgcgaagcg ttgtgcaca agaatcgcc 
      atcatggta aatggcaca ttccagtgt ctgggatcc 
      acccaacga ttgaaaaat aaatttgcg caaggctat 
      gccctcacg ataaaagtg aagaagcac agcgataac 
      gtctctctt gatgatgaa attaccgtc atcgatagg 
      ttcattcag acacacttt ccgggagct gaactaaaa 
      gaaaagtat caagagttg gtgtcgtat cagttggtt 
      aacaatagt tcgttaacg tggtcgaag atgtttggg 
      attttggag aggggcaag aaagatctc aacatcgag 
      gactattcg ttgggtcag tgcagtcta gaacaagtc 
      tttttgagt tttacaaag taccaaaac tag
    mRNA sequence
    • >TC031677 RA
      tgtgtgtga tactgactt aagactgtg cagaaatga 
      gccaaatat aaacttaag gttgattga aagttatta 
      gatattttt tttattttt cgcttctaa aagtgaaaa 
      aaatggcta accgtttaa ataaattta tattgttga 
      tgtggaaaa attggacgc ttttgcgac gacgtccga 
      ttcaagcag catttgaga ttatttatc ctgtattga 
      tatgtttaa ttcttgttg ctgtaagag gcttaatta 
      atgttagta aggaggggc caatcagtt atgacccat 
      ttataccgg catcgacaa tgggagaat tatgttctt 
      ggagttttc aagacggtc ataggacta atattacca 
      tggcgtatt cgccaacca gcccactac tagaaaaag 
      tgttcgaaa atctttgtc tcaacgtta caccaccac 
      ccaatttaa ttcttaaaa gttatagaa ctgcagctg 
      aaatgaacg ctgatatac cagataata gatttaatt 
      ttgctttac aattggacg attctttat ttggacttg 
      atgacagca gcaaactgc ctgaaaatg tggatgtca 
      ctttacggt ttccatcag aagattcaa ttgtttcta 
      gacgagagt ggtacacta atttaatat acccgcttt 
      tacaaacac caggcccaa gatcgagtc aggacaact 
      ggggtggag aaccagggt atgtcaacc gaggatttc 
      tctatgccc aagctatta ttactaaaa tatttatta 
      ataacggcg aaaatattc ctttgaata cagaagtgg 
      ttatgcagc gattccctt accctgaat ggttaaacg 
      atgcctttt accttgatc aaatggaaa cgatggtat 
      cgctgatga taatgatga gttttatgt ttaactatg 
      ttaacgcta taagggcca taacaacag aaaaagaaa 
      agcaactca aggaatcta tgaaagtta tgggcctcc 
      ctgggtggt tacattgga tggcatggt tcctacgta 
      gctttgtta ttctcctaa tagcaataa ttctaatca 
      caattgtct taaaagtaa atttccaaa agaatgcag 
      tctttgtgc actccgacg gcactgtct tatttgtgt 
      tttttatac tttttgcat gcagcacca tcacgttca 
      cttttctaa taagcgtgt ttttttcga aagcaaata 
      ctgcaacag ccgttggca gtctggtat tctttctta 
      catatctgc catacacac tgcaacaac agcaaggtg 
      atatagtgt ctttaggtt ctgcactaa gttcaagtc 
      ttttggcca actctggat tgtcttttg ccatctctg 
      ttattctaa aatttgaag caattgaag aaggaagtc 
      aatggagta atttgtttt caactgtat cccccgatg 
      ataacttaa cacttggtg cattcttct taatgttta 
      tcctcgaca cattcctct atttgtgta tagctttat 
      acatagaag cagtgtttc ccggtgact ttggagtgc 
      cacaacctt ggtacttcc cttttacga gagcttact 
      ggtgtaaca atatccagg ctatgggtg atgaacagt 
      cgtctgaga ataagggag aattctatg aagatttca 
      ccgaaaaac tacctgttg gtatacaat tgaagaacc 
      tttcaaaaa catttggag gcaataaag cagttataa 
      atatgaatt tggatatgt atgaaggcc atatcactg 
      ttttgcttg gccacaatg gtgcaggca aaaccacaa 
      caatgtcta tgataaccg gaatgtttc cacctacta 
      gtggaaccg ctatagtta gcggatatg atgtaagaa 
      ccagtatac aaaatgttc gggacagta tgggtttgt 
      gccttcaac acaacgtct tgtttgata acttaactg 
      tttgggaac acttgtatt tctttggta aattaaaag 
      gtttaagaa acgacgaaa ttaatgagg aaattgatc 
      gttacctga agctacttg agctggaag ataagagag 
      atgcacatt caagtactc tgtctggag gtatgaagc 
      gaaagttaa gtgtaggaa tggcgcttt gtggaaaat 
      ctaaagtag tgatgcttg atgaaccaa cagcaggga 
      tggaccctt cagcaagaa gagcgattt ggaatttac 
      ttcaaaagc aaaagagcg gacgaacaa ttctactaa 
      caacacact acatggatg aagcagatc tattaggag 
      ataggattg ctataatga caggtggcg aattacaat 
      gttgcggat caagtttct tcttaaaga agaaatacg 
      gcgctggtt attacctaa tcatggatg taacaccaa 
      attgccaac cgtccaaaa ttacacagc tgctgcaaa 
      attatatac caaacgtgc aaatacatt ctcacgtag 
      gttctgagc tgacttatc aactacccg aaaatgaat 
      catggaaat ttgaaaaaa tgctaggcc agttagaaa 
      atgaaagta ccagtctgg gtgtgcaaa gttacggag 
      tatcgctaa caacacttg aagaagttt ttatgaaag 
      tcggtgccg accatgata ataaacttt cgaaaaaaa 
      taaatggaa acagtgaaa gtacaataa ctgtggaag 
      aatctggtt taagtagtt taaacatcg cccatctga 
      gtggtttca acttactga ggaatcagt tcatagcca 
      ttgtcctga agaaaaact tatctcttc taagatctt 
      ggtacttgt tcctactcc aagttattt tacctgtgg 
      cacttttga taatcacag ttctaaatg cccgaggtt 
      acgcaccca aaacttcgt ttcccgcct taaaaatgt 
      ctctagaat cgtacaatg aaccaataa cactagtcc 
      aaaatgatg gcagttcac tggccaata catacataa 
      atatgctaa atgggtacc cagcaaaaa cagttaata 
      atcttgaat cggaaatca taaatttga caaaagagt 
      caccatcaa ctgtaaaaa ggcactata ctgttgcgg 
      cttctttca acagtacag ttgccaccg cttggttta 
      acggtgatc cttatcact caacatcac tatccctca 
      gtcttgtgt tgaatactg tttacaaga gcaaatttg 
      gagaaaaaa aaaccctta attttatta atcacccgc 
      ttcctctga gcaaggaag ctgaactca acaatatgc 
      aatttaacc ttatggtgt accaaatag caatggaac 
      taggatacg gaatggctt tcgttgcta gtttctaca 
      ttttatttt atatccgag aacgagtta gtaaatcca 
      aacacctgc agttcgttt ctggtgtaa atgctttcg 
      ttttctggg gaactgctt tcctttgcg atctaatca 
      catatctga taacagtca tttttgttc tcatcacat 
      ttgcagttc tgcaggaag acggtttta agacaacgg 
      acgaaatag gtcgcataa ctttagtgc ttgtttatt 
      ttggcttct ttgtcttac catttgttt atttaacat 
      cgtacttgt tcagtattc catcttccg gattttcta 
      gaatgatgt tgttgggag cagtttctg gcattgttg 
      gaattacgg ccatgcaag ttttggaaa ttgaagctc 
      taggtgttt tatatgtgg cacgacctc tccattggg 
      tacttcttt ttgttcctt tctacactg tagctaaag 
      gagcttatg atgtgggtt caataagta ctttgagag 
      atgtttgtc taaaaaatg gtgcaagtt atgaacaag 
      cttgtgccg ccaactcaa tgtgttgca atattgaca 
      actactatt cttttgata cacccggta ttgggcgca 
      atatagtta tttcctttc ttatgtcgt ttatcctct 
      ttgctgtac tcttaatca acgaatatg gtttgttca 
      gttacgtta tgaacaaaa taattaatt acaataaac 
      cgccacttc aaaacgtat ctctcgaaa gtgatgtgc 
      aagaagaaa acgaaaaaa ttcgaaata catctgaat 
      acgacctca cgaaaacct actctctcg ttctccgag 
      atgtcacaa agtattaca agaacttcc ttgcggtta 
      atggccttt gcttgggtg taaaaccct acgaatgct 
      tcgggttat tgggagtta acggtgctg gtaaaacta 
      ccactttca aaatgatga ccggcgatg aacaaatat 
      cgtatggtg aggcctggg tgctaggac acaacatta 
      aaacggaac aaaagcaag tccaaaaac tgatcggtt 
      attgtcccc agtttgatg ccttgttag atgacctca 
      cggtcaaag aaacactgc tgatttttg gcctcatcc 
      gaggaattc cttacaaga aatgcatcc cgttggccg 
      taaatctag cccatgaat tcgacttct ataaacaca 
      tccacaaaa aagtgaagg aactcagcg gaggaaata 
      agcgaaaac tgagcactg ctcttgcct tgatcgggg 
      atcccccga ttatttatc tcgatgagc ccaccgcag 
      gaatggacc cagcaacaa aacggtttc tatggacag 
      cgctagcta agttgaggg atcgaggaa agtgcatcg 
      ttttgacgt cacacagta tggaagagt gcgaagcgt 
      tgtgcacaa gaatcgcca tcatggtaa atggcacat 
      tccagtgtc tgggatcca cccaacgat tgaaaaata 
      aatttgcgc aaggctatg ccctcacga taaaagtga 
      agaagcaca gcgataacg tctctcttg atgatgaaa 
      ttaccgtca tcgataggt tcattcaga cacactttc 
      cgggagctg aactaaaag aaaagtatc aagagttgg 
      tgtcgtatc agttggtta acaatagtt cgttaacgt 
      ggtcgaaga tgtttggga ttttggaga ggggcaaga 
      aagatctca acatcgagg actattcgt tgggtcagt 
      gcagtctag aacaagtct ttttgagtt ttacaaagt 
      accaaaact agtacgatt atttgtcat ttgacaaga 
      aagaacgta tttaggtaa ttttcaaat ttatattta 
      ttacaaaca taacttact gttgctgta acgtacaaa 
      atacatagt atttatttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY