| General information | |||
| TC002280 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 202960..218423 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002280 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06087 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC002280 RA
MGKKFNTFL LLMWKNWKL QYRRPLQTT IEITAPIIF SILLVMLRS LVEPVDNPT REYQPFHPI PAEFFAQKK VKNQTVQSY LENLSIVYS PSPHKIIDQ AMGFLKTTF RNIKGYKNA KLLEAHFLT DEGRKTLAG IQFDDNLYN QTELPQKLQ ISIRFPGEL RSVGGYLFK NQKQGTNVS VNNSHNESE SIVDKLNKL FQIHDWKTN LLYPVFQSP GPREPEKNS GATPSYHLE GFLALQILI TYFLLLTQN EVYSLLQRE TALNIQNPV ILLQRFPHA AWREDPLLP ALISFIGIL IMLSFVYTC INTVKVITV EKEKQLKEV MKIMGLSNW IHWTAWFTK CFLFLLISI SFMTALLTI SWYPQTTFC IFTYSNPII LFLFLLFYV CATITFCFS VSVFFSKAN TAATVAGLL WFLSYAPYL FLQSQYEQL SLSTKMLVS LGSNTAMAY GFQLFLMFE STTEGIQWH NMWQPITQD DSLTLGLIL VMLAIDTLI YLFIALYIE AVFPGDFGT PQPWYFPLT CSYWCGYPK GAHVHPFDD SKQDEEFFE KNENLNPKI EIRNLRKVF GIIPPTSGI AMILGRDIQ TNMKNIRAN IGLCPQHNI LFDELTVKE HLYFFCKLK GLSSVEIEK EIGKYVNLL ELQPKINKK STTLSGGMK RKLCVAIAL CGGSKIVLL DEPTAGMDP SSRRALWDL LQVQKNERS ILLTTHYMD EADLLGDRI AIMANGELK CCGTSFFLK KKYGAGYHL ILEKLPNCN PKKITELLK KYIPDIQIH GNVGSELSY LLKENYVAY FENLLYDLE INTENLGIR SYGISLTTL EDVFLKVGA DPSGNDLCK NISSTKTMG LQGRISPKN ITLSTGLEL IRNQFIAML MKKTLTFMR TWLLQLVQI IIPVAFLII AIVVSRNLN KNKFEDLPK LLLTPHSYK NPIFLVEDH FKNQFSQNY FKNLEGFEV KSVTDLSTA ILNLSRTIP AIVKKKYIV GASFAYDRN KSPVLTAWF NNNPYHTPA LSFGLVLNS MYQVIMGPN HSIVFANHP LPFTMNTKV DRLLKGNSM GFQVAFNIG FSMSFVSSF YILFYVKER VCKAKHLQF LSGVKGYIF WTTSFLCDL TTFAVTILA VIITLLCFG EEGFSNVDE LGRLSFLLF YFGFAMLPM MYFASYYFE VPSTGFTRM TLFNVCFGV TAFLVVQIL LTPGLFLKY VADALHWCF LLIPHYSLA TGIRDTYIT FATSKMCNF LVTNCMEYV ANTTRDKCW DLACHNYSA SLSHYCCEK VESFFKWEK PGIGRNITY SLLTGIMLF IFVILCEYK IVAKMRCFR NYGIRTFLK VPYEDQDVL DEKIKIRNS PESKLLKEY DLLLKDLTK YYRTFLAVN GLCLGVKQY ECFGLLGIN GAGMDPVTK RNLWNALCT VRDNGKCIL LTSHSMEEC EALCTRLAI MVNGDFKCL GSVQHLKNK FAAGFTLTI KVKREESSE TIYNFTKID KFVAENFPG IVQQEKHQE LATYYIIDH NESWSRMFG ILESARSRL NIEDYFLGQ ASLEQIFLT LTQLQR
- >TC002280 RA
atggggaaa aaatttaat acgttcctt ttactaatg tggaaaaat tggaaactg cagtatcga agacctcta caaaccacg atcgaaata actgcacct atcattttc tcgattctt ctagttatg ctcagaagt ttagtagaa ccggtggac aacccaaca cgcgaatac cagcctttc catccaatc cccgcagaa ttttttgcc caaaagaag gtcaaaaat caaacagtg cagtcatac cttgaaaat ctaagtata gtatattca ccatctcca cacaaaatt atagaccaa gcaatgggt tttttaaaa acaactttc cgaaacatt aaaggctac aaaaacgcc aaactttta gaagcccac tttctcacc gacgaaggt cgtaagacc ttagctggg atccagttc gacgacaac ttgtataac caaacggaa cttccgcaa aaattgcaa atttcgata cgttttcca ggtgaattg cgcagtgtt ggtggttat cttttcaaa aatcaaaaa caaggaaca aatgtttct gtaaataat tcccacaat gagtctgaa tctatagtt gacaaatta aacaaactg tttcaaatt cacgattgg aaaacaaac ttgttgtac cccgttttc caaagtcca ggacccaga gaaccggag aaaaacagt ggtgctacc ccaagttat catttggag gggtttttg gctctacaa atactaata acctacttc cttctcctt acacaaaat gaagtttac tcgctactt caacgagaa acagcttta aatatccaa aatccagtt attctcttg caaagattc ccacatgct gcttggaga gaagatccc ttattacca gctttgatc agcttcatc ggtattctc ataatgcta agtttcgtc tacacatgc attaacaca gtaaaggta attactgtc gaaaaagag aagcaattg aaggaagta atgaagata atgggtttg tcaaactgg atacactgg actgcttgg ttcacaaag tgttttcta tttcttttg atttcaata agtttcatg accgcattg ctaacaatt tcgtggtat ccgcaaaca acattttgc atcttcact tattctaat cccataatc ttatttctg tttttgttg ttttacgtt tgtgcgacc atcactttt tgtttttcg gtcagtgtt tttttctcg aaagctaac actgctgcc accgttgct gggctgttg tggtttctt tcttacgcc ccatattta ttccttcag tcacaatac gaacaacta agtttatcg actaaaatg ttagtaagt ttgggatct aacacggca atggcttac ggatttcaa ctcttcttg atgtttgaa agtaccact gaaggaatc cagtggcac aacatgtgg caaccgatc actcaggac gattctctc actcttgga ctgatattg gtcatgttg gctatcgat accttgatt tatttattt atcgctctg tatatcgag gctgttttt cctggcgat tttgggaca ccacaaccg tggtatttc ccattgact tgttcgtat tggtgcggt tatccaaaa ggtgctcat gttcaccct tttgacgac tcgaaacaa gatgaagaa tttttcgag aaaaatgaa aatttaaat ccaaaaata gagatcaga aacttgcga aaagttttc ggaatcatt ccaccgacg agtggaata gcaatgatc ttaggacgt gatattcaa acgaacatg aaaaatatc agagccaat ataggactc tgccctcag cacaatata cttttcgat gagttgaca gtaaaggaa catttgtat ttcttctgt aaactcaaa ggtctcagt agcgtcgag atcgagaaa gaaattggg aagtatgtc aatcttttg gaactgcaa ccaaaaatc aataaaaaa tccacaacc ttgtcggga ggcatgaaa cgcaaactc tgcgtcgct attgcactg tgtggtgga tcgaaaatt gttcttctc gatgaacca acagctgga atggatcct tcctccaga agagcacta tgggacttg cttcaagtt caaaagaat gaaaggagt atactatta acaacgcac tacatggac gaagctgat ttgttgggg gacaggatc gcaatcatg gcaaatgga gaattgaaa tgttgtggt acaagcttt ttcctaaag aagaaatat ggtgctggc tatcacttg atactagaa aagcttcca aactgcaac ccgaaaaaa atcaccgaa cttttgaaa aaatacatt ccagacatt caaatacat ggaaatgta ggctccgaa ctcagttat ttgttgaaa gaaaattat gtcgcgtat ttcgaaaat ctgctctat gatttagaa ataaataca gaaaattta ggcattcgc agctacgga atatctcta acgacttta gaggatgta tttttgaaa gtcggagct gatccaagt ggcaatgat ttatgtaaa aatatttct tcaactaaa acgatgggg ctgcaggga aggatttcg ccaaaaaac ataacactt tcgactggt ctggaactg attcgaaat cagttcatc gctatgtta atgaaaaaa actttaact tttatgcga acgtggtta ctacaactc gttcaaata atcatcccg gtggctttt ctgataatt gccatagtt gttagtcgg aatttaaac aagaataaa tttgaagat ttacccaaa ttgcttctc actccacat tcctacaaa aatccgata tttcttgta gaggatcat ttcaagaac cagtttagt caaaattat tttaagaat ttggagggt tttgaggtc aaatctgtg accgatctt agcacagca atactgaat ttgtcaaga acaattcca gccattgtg aagaaaaag tacattgtt ggtgcctct tttgcatat gatcggaac aaaagtcct gttttaaca gcctggttc aacaataat ccgtatcac acacctgct ttgtctttc ggacttgtg cttaactcc atgtatcaa gtgatcatg ggcccgaat cacagcatt gtcttcgca aatcaccca ctgcctttt acaatgaac accaaggtc gatcggttg ctcaaaggc aacagtatg ggtttccag gttgcgttc aatataggc ttcagcatg tctttcgtt tcgtcgttc tacattttg ttttacgta aaagaacgg gtttgtaaa gccaaacat ctgcaattt ttgtcgggc gtaaaaggt tacattttc tggacgact tcattcctg tgtgatttg acgactttt gctgtaact atactagca gtcatcatc actttgttg tgttttggg gaagaaggg ttcagtaat gtcgatgaa ttaggtagg ctctccttc ctcttgttt tactttggt tttgcgatg ttgccaatg atgtacttt gcatcgtac tattttgaa gtaccatca actggattc actcgaatg acacttttt aacgtgtgt tttggtgtt actgccttc ttggtggtg caaattcta ctaacaccg ggtttattc ttaaaatat gtagcagat gctctccat tggtgcttc cttttgatc ccccattac tccttagcc actggtatc agagacacg tacattaca tttgcaact tcaaaaatg tgcaatttt cttgttacc aattgtatg gaatatgta gcgaatacg acccgtgat aaatgttgg gatttggcg tgtcataac tattcagct agtttatca cactattgt tgtgaaaaa gttgaaagt tttttcaag tgggaaaaa ccgggaatc ggccgtaac atcacctat tcactccta acaggaata atgttattt atatttgta attttatgt gaatacaag atcgtagcg aaaatgaga tgttttcgt aattatggc attagaaca tttctaaag gtgccatac gaagaccaa gacgtcttg gacgaaaaa attaagatt cgaaatagc cccgaatca aaattgctc aaagaatac gatctgttg ttgaaagac ttgacgaaa tattataga accttccta gcggtcaat ggtttatgt cttggggta aaacagtac gagtgtttt gggcttttg gggataaac ggtgctggt atggatcct gtgactaaa agaaacttg tggaatgcc ctttgcaca gtccgggat aatggaaaa tgtatctta cttacgtcc cacagtatg gaagaatgt gaagctctg tgcacaaga ttggccatt atggtaaac ggagacttc aagtgtttg ggttctgtt caacatttg aaaaataaa tttgcggct ggatttact ctcactatt aaagtaaag cgggaagaa agcagcgaa acgatttat aattttact aaaatcgac aaatttgtg gctgagaat tttccgggg atagtgcag caagaaaaa catcaagaa ttggctact tattacata atcgatcat aatgaaagt tggtctaga atgtttgga attctcgaa agtgccagg agtagactc aacattgaa gattatttt ctgggacaa gctagcttg gaacagata ttcctgaca ctcacccaa ctccagcgt tga
- >TC002280 RA
agctcaaac ttgtcttaa attacagag taaatcctg agctgaagt gcaaaacat tgactgtcg ctttacaca acaatgggg aaaaaattt aatacgttc cttttacta atgtggaaa aattggaaa ctgcagtat cgaagacct ctacaaacc acgatcgaa ataactgca cctatcatt ttctcgatt cttctagtt atgctcaga agtttagta gaaccggtg gacaaccca acacgcgaa taccagcct ttccatcca atccccgca gaatttttt gcccaaaag aaggtcaaa aatcaaaca gtgcagtca taccttgaa aatctaagt atagtatat tcaccatct ccacacaaa attatagac caagcaatg ggtttttta aaaacaact ttccgaaac attaaaggc tacaaaaac gccaaactt ttagaagcc cactttctc accgacgaa ggtcgtaag accttagct gggatccag ttcgacgac aacttgtat aaccaaacg gaacttccg caaaaattg caaatttcg atacgtttt ccaggtgaa ttgcgcagt gttggtggt tatcttttc aaaaatcaa aaacaagga acaaatgtt tctgtaaat aattcccac aatgagtct gaatctata gttgacaaa ttaaacaaa ctgtttcaa attcacgat tggaaaaca aacttgttg taccccgtt ttccaaagt ccaggaccc agagaaccg gagaaaaac agtggtgct accccaagt tatcatttg gaggggttt ttggctcta caaatacta ataacctac ttccttctc cttacacaa aatgaagtt tactcgcta cttcaacga gaaacagct ttaaatatc caaaatcca gttattctc ttgcaaaga ttcccacat gctgcttgg agagaagat cccttatta ccagctttg atcagcttc atcggtatt ctcataatg ctaagtttc gtctacaca tgcattaac acagtaaag gtaattact gtcgaaaaa gagaagcaa ttgaaggaa gtaatgaag ataatgggt ttgtcaaac tggatacac tggactgct tggttcaca aagtgtttt ctatttctt ttgatttca ataagtttc atgaccgca ttgctaaca atttcgtgg tatccgcaa acaacattt tgcatcttc acttattct aatcccata atcttattt ctgtttttg ttgttttac gtttgtgcg accatcact ttttgtttt tcggtcagt gtttttttc tcgaaagct aacactgct gccaccgtt gctgggctg ttgtggttt ctttcttac gccccatat ttattcctt cagtcacaa tacgaacaa ctaagttta tcgactaaa atgttagta agtttggga tctaacacg gcaatggct tacggattt caactcttc ttgatgttt gaaagtacc actgaagga atccagtgg cacaacatg tggcaaccg atcactcag gacgattct ctcactctt ggactgata ttggtcatg ttggctatc gataccttg atttattta tttatcgct ctgtatatc gaggctgtt tttcctggc gattttggg acaccacaa ccgtggtat ttcccattg acttgttcg tattggtgc ggttatcca aaaggtgct catgttcac ccttttgac gactcgaaa caagatgaa gaatttttc gagaaaaat gaaaattta aatccaaaa atagagatc agaaacttg cgaaaagtt ttcggaatc attccaccg acgagtgga atagcaatg atcttagga cgtgatatt caaacgaac atgaaaaat atcagagcc aatatagga ctctgccct cagcacaat atacttttc gatgagttg acagtaaag gaacatttg tatttcttc tgtaaactc aaaggtctc agtagcgtc gagatcgag aaagaaatt gggaagtat gtcaatctt ttggaactg caaccaaaa atcaataaa aaatccaca accttgtcg ggaggcatg aaacgcaaa ctctgcgtc gctattgca ctgtgtggt ggatcgaaa attgttctt ctcgatgaa ccaacagct ggaatggat ccttcctcc agaagagca ctatgggac ttgcttcaa gttcaaaag aatgaaagg agtatacta ttaacaacg cactacatg gacgaagct gatttgttg ggggacagg atcgcaatc atggcaaat ggagaattg aaatgttgt ggtacaagc tttttccta aagaagaaa tatggtgct ggctatcac ttgatacta gaaaagctt ccaaactgc aacccgaaa aaaatcacc gaacttttg aaaaaatac attccagac attcaaata catggaaat gtaggctcc gaactcagt tatttgttg aaagaaaat tatgtcgcg tatttcgaa aatctgctc tatgattta gaaataaat acagaaaat ttaggcatt cgcagctac ggaatatct ctaacgact ttagaggat gtatttttg aaagtcgga gctgatcca agtggcaat gatttatgt aaaaatatt tcttcaact aaaacgatg gggctgcag ggaaggatt tcgccaaaa aacataaca ctttcgact ggtctggaa ctgattcga aatcagttc atcgctatg ttaatgaaa aaaacttta acttttatg cgaacgtgg ttactacaa ctcgttcaa ataatcatc ccggtggct tttctgata attgccata gttgttagt cggaattta aacaagaat aaatttgaa gatttaccc aaattgctt ctcactcca cattcctac aaaaatccg atatttctt gtagaggat catttcaag aaccagttt agtcaaaat tattttaag aatttggag ggttttgag gtcaaatct gtgaccgat cttagcaca gcaatactg aatttgtca agaacaatt ccagccatt gtgaagaaa aagtacatt gttggtgcc tcttttgca tatgatcgg aacaaaagt cctgtttta acagcctgg ttcaacaat aatccgtat cacacacct gctttgtct ttcggactt gtgcttaac tccatgtat caagtgatc atgggcccg aatcacagc attgtcttc gcaaatcac ccactgcct tttacaatg aacaccaag gtcgatcgg ttgctcaaa ggcaacagt atgggtttc caggttgcg ttcaatata ggcttcagc atgtctttc gtttcgtcg ttctacatt ttgttttac gtaaaagaa cgggtttgt aaagccaaa catctgcaa tttttgtcg ggcgtaaaa ggttacatt ttctggacg acttcattc ctgtgtgat ttgacgact tttgctgta actatacta gcagtcatc atcactttg ttgtgtttt ggggaagaa gggttcagt aatgtcgat gaattaggt aggctctcc ttcctcttg ttttacttt ggttttgcg atgttgcca atgatgtac tttgcatcg tactatttt gaagtacca tcaactgga ttcactcga atgacactt tttaacgtg tgttttggt gttactgcc ttcttggtg gtgcaaatt ctactaaca ccgggttta ttcttaaaa tatgtagca gatgctctc cattggtgc ttccttttg atcccccat tactcctta gccactggt atcagagac acgtacatt acatttgca acttcaaaa atgtgcaat tttcttgtt accaattgt atggaatat gtagcgaat acgacccgt gataaatgt tgggatttg gcgtgtcat aactattca gctagttta tcacactat tgttgtgaa aaagttgaa agttttttc aagtgggaa aaaccggga atcggccgt aacatcacc tattcactc ctaacagga ataatgtta tttatattt gtaatttta tgtgaatac aagatcgta gcgaaaatg agatgtttt cgtaattat ggcattaga acatttcta aaggtgcca tacgaagac caagacgtc ttggacgaa aaaattaag attcgaaat agccccgaa tcaaaattg ctcaaagaa tacgatctg ttgttgaaa gacttgacg aaatattat agaaccttc ctagcggtc aatggttta tgtcttggg gtaaaacag tacgagtgt tttgggctt ttggggata aacggtgct ggtatggat cctgtgact aaaagaaac ttgtggaat gccctttgc acagtccgg gataatgga aaatgtatc ttacttacg tcccacagt atggaagaa tgtgaagct ctgtgcaca agattggcc attatggta aacggagac ttcaagtgt ttgggttct gttcaacat ttgaaaaat aaatttgcg gctggattt actctcact attaaagta aagcgggaa gaaagcagc gaaacgatt tataatttt actaaaatc gacaaattt gtggctgag aattttccg gggatagtg cagcaagaa aaacatcaa gaattggct acttattac ataatcgat cataatgaa agttggtct agaatgttt ggaattctc gaaagtgcc aggagtaga ctcaacatt gaagattat tttctggga caagctagc ttggaacag atattcctg acactcacc caactccag cgttgattc aagtaatgt aatgcagaa attctggtc ttgtaataa ataaaactc caaatt
