Status
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Gene information
General information
TC002280 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 202960..218423
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002280
    iBeetle screen iB_06087

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002280 RA
      MGKKFNTFL LLMWKNWKL QYRRPLQTT IEITAPIIF 
      SILLVMLRS LVEPVDNPT REYQPFHPI PAEFFAQKK 
      VKNQTVQSY LENLSIVYS PSPHKIIDQ AMGFLKTTF 
      RNIKGYKNA KLLEAHFLT DEGRKTLAG IQFDDNLYN 
      QTELPQKLQ ISIRFPGEL RSVGGYLFK NQKQGTNVS 
      VNNSHNESE SIVDKLNKL FQIHDWKTN LLYPVFQSP 
      GPREPEKNS GATPSYHLE GFLALQILI TYFLLLTQN 
      EVYSLLQRE TALNIQNPV ILLQRFPHA AWREDPLLP 
      ALISFIGIL IMLSFVYTC INTVKVITV EKEKQLKEV 
      MKIMGLSNW IHWTAWFTK CFLFLLISI SFMTALLTI 
      SWYPQTTFC IFTYSNPII LFLFLLFYV CATITFCFS 
      VSVFFSKAN TAATVAGLL WFLSYAPYL FLQSQYEQL 
      SLSTKMLVS LGSNTAMAY GFQLFLMFE STTEGIQWH 
      NMWQPITQD DSLTLGLIL VMLAIDTLI YLFIALYIE 
      AVFPGDFGT PQPWYFPLT CSYWCGYPK GAHVHPFDD 
      SKQDEEFFE KNENLNPKI EIRNLRKVF GIIPPTSGI 
      AMILGRDIQ TNMKNIRAN IGLCPQHNI LFDELTVKE 
      HLYFFCKLK GLSSVEIEK EIGKYVNLL ELQPKINKK 
      STTLSGGMK RKLCVAIAL CGGSKIVLL DEPTAGMDP 
      SSRRALWDL LQVQKNERS ILLTTHYMD EADLLGDRI 
      AIMANGELK CCGTSFFLK KKYGAGYHL ILEKLPNCN 
      PKKITELLK KYIPDIQIH GNVGSELSY LLKENYVAY 
      FENLLYDLE INTENLGIR SYGISLTTL EDVFLKVGA 
      DPSGNDLCK NISSTKTMG LQGRISPKN ITLSTGLEL 
      IRNQFIAML MKKTLTFMR TWLLQLVQI IIPVAFLII 
      AIVVSRNLN KNKFEDLPK LLLTPHSYK NPIFLVEDH 
      FKNQFSQNY FKNLEGFEV KSVTDLSTA ILNLSRTIP 
      AIVKKKYIV GASFAYDRN KSPVLTAWF NNNPYHTPA 
      LSFGLVLNS MYQVIMGPN HSIVFANHP LPFTMNTKV 
      DRLLKGNSM GFQVAFNIG FSMSFVSSF YILFYVKER 
      VCKAKHLQF LSGVKGYIF WTTSFLCDL TTFAVTILA 
      VIITLLCFG EEGFSNVDE LGRLSFLLF YFGFAMLPM 
      MYFASYYFE VPSTGFTRM TLFNVCFGV TAFLVVQIL 
      LTPGLFLKY VADALHWCF LLIPHYSLA TGIRDTYIT 
      FATSKMCNF LVTNCMEYV ANTTRDKCW DLACHNYSA 
      SLSHYCCEK VESFFKWEK PGIGRNITY SLLTGIMLF 
      IFVILCEYK IVAKMRCFR NYGIRTFLK VPYEDQDVL 
      DEKIKIRNS PESKLLKEY DLLLKDLTK YYRTFLAVN 
      GLCLGVKQY ECFGLLGIN GAGMDPVTK RNLWNALCT 
      VRDNGKCIL LTSHSMEEC EALCTRLAI MVNGDFKCL 
      GSVQHLKNK FAAGFTLTI KVKREESSE TIYNFTKID 
      KFVAENFPG IVQQEKHQE LATYYIIDH NESWSRMFG 
      ILESARSRL NIEDYFLGQ ASLEQIFLT LTQLQR
    CDS sequence
    • >TC002280 RA
      atggggaaa aaatttaat acgttcctt ttactaatg 
      tggaaaaat tggaaactg cagtatcga agacctcta 
      caaaccacg atcgaaata actgcacct atcattttc 
      tcgattctt ctagttatg ctcagaagt ttagtagaa 
      ccggtggac aacccaaca cgcgaatac cagcctttc 
      catccaatc cccgcagaa ttttttgcc caaaagaag 
      gtcaaaaat caaacagtg cagtcatac cttgaaaat 
      ctaagtata gtatattca ccatctcca cacaaaatt 
      atagaccaa gcaatgggt tttttaaaa acaactttc 
      cgaaacatt aaaggctac aaaaacgcc aaactttta 
      gaagcccac tttctcacc gacgaaggt cgtaagacc 
      ttagctggg atccagttc gacgacaac ttgtataac 
      caaacggaa cttccgcaa aaattgcaa atttcgata 
      cgttttcca ggtgaattg cgcagtgtt ggtggttat 
      cttttcaaa aatcaaaaa caaggaaca aatgtttct 
      gtaaataat tcccacaat gagtctgaa tctatagtt 
      gacaaatta aacaaactg tttcaaatt cacgattgg 
      aaaacaaac ttgttgtac cccgttttc caaagtcca 
      ggacccaga gaaccggag aaaaacagt ggtgctacc 
      ccaagttat catttggag gggtttttg gctctacaa 
      atactaata acctacttc cttctcctt acacaaaat 
      gaagtttac tcgctactt caacgagaa acagcttta 
      aatatccaa aatccagtt attctcttg caaagattc 
      ccacatgct gcttggaga gaagatccc ttattacca 
      gctttgatc agcttcatc ggtattctc ataatgcta 
      agtttcgtc tacacatgc attaacaca gtaaaggta 
      attactgtc gaaaaagag aagcaattg aaggaagta 
      atgaagata atgggtttg tcaaactgg atacactgg 
      actgcttgg ttcacaaag tgttttcta tttcttttg 
      atttcaata agtttcatg accgcattg ctaacaatt 
      tcgtggtat ccgcaaaca acattttgc atcttcact 
      tattctaat cccataatc ttatttctg tttttgttg 
      ttttacgtt tgtgcgacc atcactttt tgtttttcg 
      gtcagtgtt tttttctcg aaagctaac actgctgcc 
      accgttgct gggctgttg tggtttctt tcttacgcc 
      ccatattta ttccttcag tcacaatac gaacaacta 
      agtttatcg actaaaatg ttagtaagt ttgggatct 
      aacacggca atggcttac ggatttcaa ctcttcttg 
      atgtttgaa agtaccact gaaggaatc cagtggcac 
      aacatgtgg caaccgatc actcaggac gattctctc 
      actcttgga ctgatattg gtcatgttg gctatcgat 
      accttgatt tatttattt atcgctctg tatatcgag 
      gctgttttt cctggcgat tttgggaca ccacaaccg 
      tggtatttc ccattgact tgttcgtat tggtgcggt 
      tatccaaaa ggtgctcat gttcaccct tttgacgac 
      tcgaaacaa gatgaagaa tttttcgag aaaaatgaa 
      aatttaaat ccaaaaata gagatcaga aacttgcga 
      aaagttttc ggaatcatt ccaccgacg agtggaata 
      gcaatgatc ttaggacgt gatattcaa acgaacatg 
      aaaaatatc agagccaat ataggactc tgccctcag 
      cacaatata cttttcgat gagttgaca gtaaaggaa 
      catttgtat ttcttctgt aaactcaaa ggtctcagt 
      agcgtcgag atcgagaaa gaaattggg aagtatgtc 
      aatcttttg gaactgcaa ccaaaaatc aataaaaaa 
      tccacaacc ttgtcggga ggcatgaaa cgcaaactc 
      tgcgtcgct attgcactg tgtggtgga tcgaaaatt 
      gttcttctc gatgaacca acagctgga atggatcct 
      tcctccaga agagcacta tgggacttg cttcaagtt 
      caaaagaat gaaaggagt atactatta acaacgcac 
      tacatggac gaagctgat ttgttgggg gacaggatc 
      gcaatcatg gcaaatgga gaattgaaa tgttgtggt 
      acaagcttt ttcctaaag aagaaatat ggtgctggc 
      tatcacttg atactagaa aagcttcca aactgcaac 
      ccgaaaaaa atcaccgaa cttttgaaa aaatacatt 
      ccagacatt caaatacat ggaaatgta ggctccgaa 
      ctcagttat ttgttgaaa gaaaattat gtcgcgtat 
      ttcgaaaat ctgctctat gatttagaa ataaataca 
      gaaaattta ggcattcgc agctacgga atatctcta 
      acgacttta gaggatgta tttttgaaa gtcggagct 
      gatccaagt ggcaatgat ttatgtaaa aatatttct 
      tcaactaaa acgatgggg ctgcaggga aggatttcg 
      ccaaaaaac ataacactt tcgactggt ctggaactg 
      attcgaaat cagttcatc gctatgtta atgaaaaaa 
      actttaact tttatgcga acgtggtta ctacaactc 
      gttcaaata atcatcccg gtggctttt ctgataatt 
      gccatagtt gttagtcgg aatttaaac aagaataaa 
      tttgaagat ttacccaaa ttgcttctc actccacat 
      tcctacaaa aatccgata tttcttgta gaggatcat 
      ttcaagaac cagtttagt caaaattat tttaagaat 
      ttggagggt tttgaggtc aaatctgtg accgatctt 
      agcacagca atactgaat ttgtcaaga acaattcca 
      gccattgtg aagaaaaag tacattgtt ggtgcctct 
      tttgcatat gatcggaac aaaagtcct gttttaaca 
      gcctggttc aacaataat ccgtatcac acacctgct 
      ttgtctttc ggacttgtg cttaactcc atgtatcaa 
      gtgatcatg ggcccgaat cacagcatt gtcttcgca 
      aatcaccca ctgcctttt acaatgaac accaaggtc 
      gatcggttg ctcaaaggc aacagtatg ggtttccag 
      gttgcgttc aatataggc ttcagcatg tctttcgtt 
      tcgtcgttc tacattttg ttttacgta aaagaacgg 
      gtttgtaaa gccaaacat ctgcaattt ttgtcgggc 
      gtaaaaggt tacattttc tggacgact tcattcctg 
      tgtgatttg acgactttt gctgtaact atactagca 
      gtcatcatc actttgttg tgttttggg gaagaaggg 
      ttcagtaat gtcgatgaa ttaggtagg ctctccttc 
      ctcttgttt tactttggt tttgcgatg ttgccaatg 
      atgtacttt gcatcgtac tattttgaa gtaccatca 
      actggattc actcgaatg acacttttt aacgtgtgt 
      tttggtgtt actgccttc ttggtggtg caaattcta 
      ctaacaccg ggtttattc ttaaaatat gtagcagat 
      gctctccat tggtgcttc cttttgatc ccccattac 
      tccttagcc actggtatc agagacacg tacattaca 
      tttgcaact tcaaaaatg tgcaatttt cttgttacc 
      aattgtatg gaatatgta gcgaatacg acccgtgat 
      aaatgttgg gatttggcg tgtcataac tattcagct 
      agtttatca cactattgt tgtgaaaaa gttgaaagt 
      tttttcaag tgggaaaaa ccgggaatc ggccgtaac 
      atcacctat tcactccta acaggaata atgttattt 
      atatttgta attttatgt gaatacaag atcgtagcg 
      aaaatgaga tgttttcgt aattatggc attagaaca 
      tttctaaag gtgccatac gaagaccaa gacgtcttg 
      gacgaaaaa attaagatt cgaaatagc cccgaatca 
      aaattgctc aaagaatac gatctgttg ttgaaagac 
      ttgacgaaa tattataga accttccta gcggtcaat 
      ggtttatgt cttggggta aaacagtac gagtgtttt 
      gggcttttg gggataaac ggtgctggt atggatcct 
      gtgactaaa agaaacttg tggaatgcc ctttgcaca 
      gtccgggat aatggaaaa tgtatctta cttacgtcc 
      cacagtatg gaagaatgt gaagctctg tgcacaaga 
      ttggccatt atggtaaac ggagacttc aagtgtttg 
      ggttctgtt caacatttg aaaaataaa tttgcggct 
      ggatttact ctcactatt aaagtaaag cgggaagaa 
      agcagcgaa acgatttat aattttact aaaatcgac 
      aaatttgtg gctgagaat tttccgggg atagtgcag 
      caagaaaaa catcaagaa ttggctact tattacata 
      atcgatcat aatgaaagt tggtctaga atgtttgga 
      attctcgaa agtgccagg agtagactc aacattgaa 
      gattatttt ctgggacaa gctagcttg gaacagata 
      ttcctgaca ctcacccaa ctccagcgt tga
    mRNA sequence
    • >TC002280 RA
      agctcaaac ttgtcttaa attacagag taaatcctg 
      agctgaagt gcaaaacat tgactgtcg ctttacaca 
      acaatgggg aaaaaattt aatacgttc cttttacta 
      atgtggaaa aattggaaa ctgcagtat cgaagacct 
      ctacaaacc acgatcgaa ataactgca cctatcatt 
      ttctcgatt cttctagtt atgctcaga agtttagta 
      gaaccggtg gacaaccca acacgcgaa taccagcct 
      ttccatcca atccccgca gaatttttt gcccaaaag 
      aaggtcaaa aatcaaaca gtgcagtca taccttgaa 
      aatctaagt atagtatat tcaccatct ccacacaaa 
      attatagac caagcaatg ggtttttta aaaacaact 
      ttccgaaac attaaaggc tacaaaaac gccaaactt 
      ttagaagcc cactttctc accgacgaa ggtcgtaag 
      accttagct gggatccag ttcgacgac aacttgtat 
      aaccaaacg gaacttccg caaaaattg caaatttcg 
      atacgtttt ccaggtgaa ttgcgcagt gttggtggt 
      tatcttttc aaaaatcaa aaacaagga acaaatgtt 
      tctgtaaat aattcccac aatgagtct gaatctata 
      gttgacaaa ttaaacaaa ctgtttcaa attcacgat 
      tggaaaaca aacttgttg taccccgtt ttccaaagt 
      ccaggaccc agagaaccg gagaaaaac agtggtgct 
      accccaagt tatcatttg gaggggttt ttggctcta 
      caaatacta ataacctac ttccttctc cttacacaa 
      aatgaagtt tactcgcta cttcaacga gaaacagct 
      ttaaatatc caaaatcca gttattctc ttgcaaaga 
      ttcccacat gctgcttgg agagaagat cccttatta 
      ccagctttg atcagcttc atcggtatt ctcataatg 
      ctaagtttc gtctacaca tgcattaac acagtaaag 
      gtaattact gtcgaaaaa gagaagcaa ttgaaggaa 
      gtaatgaag ataatgggt ttgtcaaac tggatacac 
      tggactgct tggttcaca aagtgtttt ctatttctt 
      ttgatttca ataagtttc atgaccgca ttgctaaca 
      atttcgtgg tatccgcaa acaacattt tgcatcttc 
      acttattct aatcccata atcttattt ctgtttttg 
      ttgttttac gtttgtgcg accatcact ttttgtttt 
      tcggtcagt gtttttttc tcgaaagct aacactgct 
      gccaccgtt gctgggctg ttgtggttt ctttcttac 
      gccccatat ttattcctt cagtcacaa tacgaacaa 
      ctaagttta tcgactaaa atgttagta agtttggga 
      tctaacacg gcaatggct tacggattt caactcttc 
      ttgatgttt gaaagtacc actgaagga atccagtgg 
      cacaacatg tggcaaccg atcactcag gacgattct 
      ctcactctt ggactgata ttggtcatg ttggctatc 
      gataccttg atttattta tttatcgct ctgtatatc 
      gaggctgtt tttcctggc gattttggg acaccacaa 
      ccgtggtat ttcccattg acttgttcg tattggtgc 
      ggttatcca aaaggtgct catgttcac ccttttgac 
      gactcgaaa caagatgaa gaatttttc gagaaaaat 
      gaaaattta aatccaaaa atagagatc agaaacttg 
      cgaaaagtt ttcggaatc attccaccg acgagtgga 
      atagcaatg atcttagga cgtgatatt caaacgaac 
      atgaaaaat atcagagcc aatatagga ctctgccct 
      cagcacaat atacttttc gatgagttg acagtaaag 
      gaacatttg tatttcttc tgtaaactc aaaggtctc 
      agtagcgtc gagatcgag aaagaaatt gggaagtat 
      gtcaatctt ttggaactg caaccaaaa atcaataaa 
      aaatccaca accttgtcg ggaggcatg aaacgcaaa 
      ctctgcgtc gctattgca ctgtgtggt ggatcgaaa 
      attgttctt ctcgatgaa ccaacagct ggaatggat 
      ccttcctcc agaagagca ctatgggac ttgcttcaa 
      gttcaaaag aatgaaagg agtatacta ttaacaacg 
      cactacatg gacgaagct gatttgttg ggggacagg 
      atcgcaatc atggcaaat ggagaattg aaatgttgt 
      ggtacaagc tttttccta aagaagaaa tatggtgct 
      ggctatcac ttgatacta gaaaagctt ccaaactgc 
      aacccgaaa aaaatcacc gaacttttg aaaaaatac 
      attccagac attcaaata catggaaat gtaggctcc 
      gaactcagt tatttgttg aaagaaaat tatgtcgcg 
      tatttcgaa aatctgctc tatgattta gaaataaat 
      acagaaaat ttaggcatt cgcagctac ggaatatct 
      ctaacgact ttagaggat gtatttttg aaagtcgga 
      gctgatcca agtggcaat gatttatgt aaaaatatt 
      tcttcaact aaaacgatg gggctgcag ggaaggatt 
      tcgccaaaa aacataaca ctttcgact ggtctggaa 
      ctgattcga aatcagttc atcgctatg ttaatgaaa 
      aaaacttta acttttatg cgaacgtgg ttactacaa 
      ctcgttcaa ataatcatc ccggtggct tttctgata 
      attgccata gttgttagt cggaattta aacaagaat 
      aaatttgaa gatttaccc aaattgctt ctcactcca 
      cattcctac aaaaatccg atatttctt gtagaggat 
      catttcaag aaccagttt agtcaaaat tattttaag 
      aatttggag ggttttgag gtcaaatct gtgaccgat 
      cttagcaca gcaatactg aatttgtca agaacaatt 
      ccagccatt gtgaagaaa aagtacatt gttggtgcc 
      tcttttgca tatgatcgg aacaaaagt cctgtttta 
      acagcctgg ttcaacaat aatccgtat cacacacct 
      gctttgtct ttcggactt gtgcttaac tccatgtat 
      caagtgatc atgggcccg aatcacagc attgtcttc 
      gcaaatcac ccactgcct tttacaatg aacaccaag 
      gtcgatcgg ttgctcaaa ggcaacagt atgggtttc 
      caggttgcg ttcaatata ggcttcagc atgtctttc 
      gtttcgtcg ttctacatt ttgttttac gtaaaagaa 
      cgggtttgt aaagccaaa catctgcaa tttttgtcg 
      ggcgtaaaa ggttacatt ttctggacg acttcattc 
      ctgtgtgat ttgacgact tttgctgta actatacta 
      gcagtcatc atcactttg ttgtgtttt ggggaagaa 
      gggttcagt aatgtcgat gaattaggt aggctctcc 
      ttcctcttg ttttacttt ggttttgcg atgttgcca 
      atgatgtac tttgcatcg tactatttt gaagtacca 
      tcaactgga ttcactcga atgacactt tttaacgtg 
      tgttttggt gttactgcc ttcttggtg gtgcaaatt 
      ctactaaca ccgggttta ttcttaaaa tatgtagca 
      gatgctctc cattggtgc ttccttttg atcccccat 
      tactcctta gccactggt atcagagac acgtacatt 
      acatttgca acttcaaaa atgtgcaat tttcttgtt 
      accaattgt atggaatat gtagcgaat acgacccgt 
      gataaatgt tgggatttg gcgtgtcat aactattca 
      gctagttta tcacactat tgttgtgaa aaagttgaa 
      agttttttc aagtgggaa aaaccggga atcggccgt 
      aacatcacc tattcactc ctaacagga ataatgtta 
      tttatattt gtaatttta tgtgaatac aagatcgta 
      gcgaaaatg agatgtttt cgtaattat ggcattaga 
      acatttcta aaggtgcca tacgaagac caagacgtc 
      ttggacgaa aaaattaag attcgaaat agccccgaa 
      tcaaaattg ctcaaagaa tacgatctg ttgttgaaa 
      gacttgacg aaatattat agaaccttc ctagcggtc 
      aatggttta tgtcttggg gtaaaacag tacgagtgt 
      tttgggctt ttggggata aacggtgct ggtatggat 
      cctgtgact aaaagaaac ttgtggaat gccctttgc 
      acagtccgg gataatgga aaatgtatc ttacttacg 
      tcccacagt atggaagaa tgtgaagct ctgtgcaca 
      agattggcc attatggta aacggagac ttcaagtgt 
      ttgggttct gttcaacat ttgaaaaat aaatttgcg 
      gctggattt actctcact attaaagta aagcgggaa 
      gaaagcagc gaaacgatt tataatttt actaaaatc 
      gacaaattt gtggctgag aattttccg gggatagtg 
      cagcaagaa aaacatcaa gaattggct acttattac 
      ataatcgat cataatgaa agttggtct agaatgttt 
      ggaattctc gaaagtgcc aggagtaga ctcaacatt 
      gaagattat tttctggga caagctagc ttggaacag 
      atattcctg acactcacc caactccag cgttgattc 
      aagtaatgt aatgcagaa attctggtc ttgtaataa 
      ataaaactc caaatt
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