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Gene information
General information
TC005119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 96290..105023
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005119
    iBeetle screen iB_06086

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 44.44% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005119 RA
      MDLLRVTSD ERTQILKLY NVDNTRLKQ DIEMITTWK 
      SKQPHLQVD VSDEFLEKL LLKNKFSVE RCKEKLENY 
      YTLRGTNKD LLEGYEKIL PSQEALTFL PLPQLTQNY 
      ERIIVMKLI NTDPKIYDL VRVFKFAVS LNEMMLRHD 
      GSLGIRVLI DYTGFTVKH LATWNPVIL MRYYSLAEK 
      AYSLRIMEL EFINYPSFV NKIFSIFRM FLRPKIYER 
      IKLHENLES LYKVIPKKY LPEDYGGTN GKISDLLEK 
      WDKEVKTQE NFLLECANT VANEELRPP ESQENDLFG 
      TGEILKLYN IDNARLKED IEVLKIWKS KQPHLEKNL 
      SDNFFEKLL LKNKFSIER CKEKLENYY TLRGKNKDL 
      LEDYEKIVP SQKAVTFLP IPELTRNYE RIVVMKIIN 
      PDLKTYDLV PAFKLALAV NEMMLRHDG SLGIRLILD 
      YTGFTLKHL STWNPIILM RYYNLAEKA YSLRILGLE 
      CINCPSFIN KVFSIFKMF LRPKIYERI KLHENLESL 
      YGVIPKEQL PKDYGGTNS SISNLLEKW DEEVEKQQS 
      FLLECANTI ANEDLRPPE SQENDLFGT GGTFKTLSL 
      D
    CDS sequence
    • >TC005119 RA
      atggactta cttcgtgta acttccgac gaaagaact 
      caaattttg aaactttac aacgttgac aatacccga 
      ttaaaacaa gacattgaa atgatcaca acttggaaa 
      tcaaaacaa ccccatctt caagttgat gtaagtgac 
      gaatttctc gagaaattg ttacttaaa aataaattc 
      agtgttgaa agatgtaaa gaaaaatta gaaaattat 
      tacacgctt cgtgggaca aataaggat ttgttggaa 
      ggttatgag aaaattcta ccgtcacaa gaagcacta 
      actttttta ccacttcct caactcacc caaaattac 
      gaacgtatc atagtcatg aaactgata aacactgat 
      ccaaaaatc tacgatctt gtacgcgtt ttcaaattt 
      gcagtaagc ctaaatgaa atgatgtta cgacatgat 
      ggcagttta ggaattcga gttcttata gactacaca 
      ggtttcact gtaaaacat ttagctact tggaatcct 
      gtaattttg atgagatat tatagcctc gctgaaaaa 
      gcttactct ctgcgaatt atggaatta gaattcatt 
      aattaccca tcgtttgtc aacaaaatt ttctcaata 
      tttaggatg tttctacga ccaaaaatt tacgaacga 
      ataaaactt cacgaaaat ctagaatct ttatacaag 
      gtaatcccc aaaaaatat ttaccggag gactatgga 
      ggtactaat ggcaaaatc tctgatttg ttagaaaaa 
      tgggacaaa gaagtaaaa acacaagaa aattttcta 
      ctagagtgt gcaaacact gtcgcaaat gaggagcta 
      cgtcctcca gaatcacaa gaaaatgat ttatttgga 
      acgggtgaa attttgaaa ctttacaac atcgacaat 
      gcccgttta aaagaagac attgaagtt cttaaaatt 
      tggaagtcc aagcaaccc catcttgaa aaaaattta 
      agcgataat ttctttgag aaattgtta cttaaaaat 
      aaattcagt atcgaacga tgtaaagag aaattggaa 
      aattattac acgcttcgt gggaagaat aaagattta 
      ctggaagat tatgaaaaa attgtaccc tcacaaaaa 
      gcagtaact tttttaccc attcccgag ctaacacga 
      aattatgaa cgtatcgtt gtcatgaaa ataataaat 
      cctgatcta aaaacttac gatcttgta ccagcgttc 
      aaacttgcc ttagctgtg aatgaaatg atgttaaga 
      catgatgga agtttagga attcggctt attttagac 
      tacacaggt ttcacgtta aaacattta agcacttgg 
      aatcctata attttaatg agatattat aatctggct 
      gaaaaagct tactctctt cgaattttg ggcttggaa 
      tgtatcaac tgtccatcg tttatcaac aaagttttc 
      tcaatattt aagatgttt ctacggcca aaaatctac 
      gaaaggata aaactgcac gaaaatttg gaatcttta 
      tacggggta attcccaaa gaacaatta ccgaaggac 
      tatggaggt actaacagt agtatctct aatttatta 
      gaaaaatgg gacgaagaa gtagaaaaa cagcaatca 
      tttttatta gagtgtgca aacactatt gctaacgaa 
      gatctccgg ccaccagaa tcacaagaa aatgattta 
      tttggaact ggaggcaca ttcaagaca ctctccctc 
      gactaa
    mRNA sequence
    • >TC005119 RA
      cagtgttca gttaattta aacatttct cgaatagtc 
      ttctaatat cgaaatgga cttacttcg tgtaacttc 
      cgacgaaag aactcaaat tttgaaact ttacaacgt 
      tgacaatac ccgattaaa acaagacat tgaaatgat 
      cacaacttg gaaatcaaa acaacccca tcttcaagt 
      tgatgtaag tgacgaatt tctcgagaa attgttact 
      taaaaataa attcagtgt tgaaagatg taaagaaaa 
      attagaaaa ttattacac gcttcgtgg gacaaataa 
      ggatttgtt ggaaggtta tgagaaaat tctaccgtc 
      acaagaagc actaacttt tttaccact tcctcaact 
      cacccaaaa ttacgaacg tatcatagt catgaaact 
      gataaacac tgatccaaa aatctacga tcttgtacg 
      cgttttcaa atttgcagt aagcctaaa tgaaatgat 
      gttacgaca tgatggcag tttaggaat tcgagttct 
      tatagacta cacaggttt cactgtaaa acatttagc 
      tacttggaa tcctgtaat tttgatgag atattatag 
      cctcgctga aaaagctta ctctctgcg aattatgga 
      attagaatt cattaatta cccatcgtt tgtcaacaa 
      aattttctc aatatttag gatgtttct acgaccaaa 
      aatttacga acgaataaa acttcacga aaatctaga 
      atctttata caaggtaat ccccaaaaa atatttacc 
      ggaggacta tggaggtac taatggcaa aatctctga 
      tttgttaga aaaatggga caaagaagt aaaaacaca 
      agaaaattt tctactaga gtgtgcaaa cactgtcgc 
      aaatgagga gctacgtcc tccagaatc acaagaaaa 
      tgatttatt tggaacggg tgaaatttt gaaacttta 
      caacatcga caatgcccg tttaaaaga agacattga 
      agttcttaa aatttggaa gtccaagca accccatct 
      tgaaaaaaa tttaagcga taatttctt tgagaaatt 
      gttacttaa aaataaatt cagtatcga acgatgtaa 
      agagaaatt ggaaaatta ttacacgct tcgtgggaa 
      gaataaaga tttactgga agattatga aaaaattgt 
      accctcaca aaaagcagt aactttttt acccattcc 
      cgagctaac acgaaatta tgaacgtat cgttgtcat 
      gaaaataat aaatcctga tctaaaaac ttacgatct 
      tgtaccagc gttcaaact tgccttagc tgtgaatga 
      aatgatgtt aagacatga tggaagttt aggaattcg 
      gcttatttt agactacac aggtttcac gttaaaaca 
      tttaagcac ttggaatcc tataatttt aatgagata 
      ttataatct ggctgaaaa agcttactc tcttcgaat 
      tttgggctt ggaatgtat caactgtcc atcgtttat 
      caacaaagt tttctcaat atttaagat gtttctacg 
      gccaaaaat ctacgaaag gataaaact gcacgaaaa 
      tttggaatc tttatacgg ggtaattcc caaagaaca 
      attaccgaa ggactatgg aggtactaa cagtagtat 
      ctctaattt attagaaaa atgggacga agaagtaga 
      aaaacagca atcattttt attagagtg tgcaaacac 
      tattgctaa cgaagatct ccggccacc agaatcaca 
      agaaaatga tttatttgg aactggagg cacattcaa 
      gacactctc cctcgacta ataactgta ttttaaaaa 
      ttgatttaa atggtaact gattattaa taataataa 
      caaagacat tttggcaag caatcaaat aaactaata 
      tttccttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY