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Gene information
General information
TC001437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3910729..3912436
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001437
    iBeetle screen iB_06085

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001437 RA
      MSSSAIYIL DVKGKVLIS RNYRGDIDL GVIEKFMPL 
      LMEKEEEGL LTPLLQTGD CTFAYIKTN NLYIVSTTK 
      KNANIALVF VFLHKIVQV MTEYFKELE EESIRDNFV 
      VIYELLDEL LDFGYPQTT DSKILQEYI TQEGHKLEI 
      QPRIPVAVT NAVSWRSEG IKYRKNEVF LDVIESVNL 
      LANANGNVL RSEIVGAIK MRVYLSGMP ELRLGLNDK 
      VLFESTGRG KSKSVELED VKFHQCVRL SRFEIDRTI 
      SFIPPDGEF ELMSYRLNT HVKPLIWIE SVIERHAHS 
      RVEYMIKAK SQFKRRSTA NNVEIVIPV PHDADSPKF 
      KTTIGSVKY APEQNAITW TIKSFPGGK EYLMRAHFG 
      LPSVECEDT EGKPPIQVK FEIPYFTTS GIQVRYLKI 
      IEKSGYQAL PWVRYITQN GDYQLRTN
    CDS sequence
    • >TC001437 RA
      atgtcgtcg tcagctatt tacattctc gacgttaag 
      ggcaaggtc cttatttca cggaactat cggggcgat 
      atagacttg ggggtgata gagaagttc atgccttta 
      cttatggaa aaggaggag gaggggctg ctcacccct 
      ctgctccaa actggcgat tgcaccttt gcctacatc 
      aagacaaac aacctgtac attgtgagc accaccaag 
      aagaacgcc aacattgcg ctagttttc gttttcttg 
      cacaagatt gtgcaagtc atgactgag tatttcaag 
      gagcttgag gaggagagt attagggac aacttcgtc 
      gttatttat gaactactg gacgaattg ctcgatttc 
      ggttacccc caaaccact gacagtaag attttacaa 
      gagtatatc acacaagag ggccacaag ttggaaata 
      cagccacgc attcccgtt gctgtaact aatgcggtt 
      tcgtggcgt tcggaaggc atcaagtat aggaaaaac 
      gaagtattt ttagacgtg atagagtcc gtcaatctc 
      ttagctaac gcgaacggt aacgtccta cgcagcgag 
      atcgtgggg gccatcaaa atgagggtc tatctctcc 
      ggcatgcct gaactgcgt ctgggtctg aacgataaa 
      gtattattc gaaagcacc ggtcgcgga aaatcaaaa 
      tcggtcgag ctagaggac gtcaaattc caccaatgt 
      gtcagacta tcgcggttt gaaatcgac aggaccata 
      tccttcatc ccgccggac ggcgaattc gaactcatg 
      tcctaccgt ttgaacaca catgtgaag ccgctaatt 
      tggatcgaa tctgtcatc gaacggcac gcacacagc 
      cgtgtcgag tacatgatc aaggcgaag tcgcagttc 
      aagcgccgc tcgacggcc aacaacgtc gaaatcgtc 
      attccggtg ccccacgac gcggactcg cccaagttc 
      aaaactacg atcgggagc gtcaaatac gcccctgag 
      caaaacgcc atcacctgg accattaag tcgttcccg 
      ggtgggaag gagtattta atgcgggcc cacttcggg 
      ctgcccagc gtcgaatgc gaagacacc gaaggcaaa 
      ccgccaatt caagtcaaa ttcgaaatt ccctacttc 
      accacgtcg ggaattcaa gtgcgctat ttgaaaatt 
      atcgaaaag agcggctac caggccttg ccgtgggtg 
      cgctacatt acgcagaac ggcgactac caactgcgc 
      accaactga
    mRNA sequence
    • >TC001437 RA
      ggcgctaac aatcaccga aatttgcaa aattaatca 
      atttaaaat cgcggtttg aatagcgcg ctacaggta 
      caccaaccg ctttaatga caggttgta gcgtaacca 
      agaaatgtg atgacggtg gttagtggc tcaaaatcg 
      tgttttatt caattttaa caaatctaa cgtctcgtt 
      agacatgat tgcgatctg acggcccga ttgaagaat 
      tttctggca ctaatcatc ctccacact aataatgtc 
      gtcgtcagc tatttacat tctcgacgt taagggcaa 
      ggtccttat ttcacggaa ctatcgggg cgatataga 
      cttgggggt gatagagaa gttcatgcc tttacttat 
      ggaaaagga ggaggaggg gctgctcac ccctctgct 
      ccaaactgg cgattgcac ctttgccta catcaagac 
      aaacaacct gtacattgt gagcaccac caagaagaa 
      cgccaacat tgcgctagt tttcgtttt cttgcacaa 
      gattgtgca agtcatgac tgagtattt caaggagct 
      tgaggagga gagtattag ggacaactt cgtcgttat 
      ttatgaact actggacga attgctcga tttcggtta 
      cccccaaac cactgacag taagatttt acaagagta 
      tatcacaca agagggcca caagttgga aatacagcc 
      acgcattcc cgttgctgt aactaatgc ggtttcgtg 
      gcgttcgga aggcatcaa gtataggaa aaacgaagt 
      atttttaga cgtgataga gtccgtcaa tctcttagc 
      taacgcgaa cggtaacgt cctacgcag cgagatcgt 
      gggggccat caaaatgag ggtctatct ctccggcat 
      gcctgaact gcgtctggg tctgaacga taaagtatt 
      attcgaaag caccggtcg cggaaaatc aaaatcggt 
      cgagctaga ggacgtcaa attccacca atgtgtcag 
      actatcgcg gtttgaaat cgacaggac catatcctt 
      catcccgcc ggacggcga attcgaact catgtccta 
      ccgtttgaa cacacatgt gaagccgct aatttggat 
      cgaatctgt catcgaacg gcacgcaca cagccgtgt 
      cgagtacat gatcaaggc gaagtcgca gttcaagcg 
      ccgctcgac ggccaacaa cgtcgaaat cgtcattcc 
      ggtgcccca cgacgcgga ctcgcccaa gttcaaaac 
      tacgatcgg gagcgtcaa atacgcccc tgagcaaaa 
      cgccatcac ctggaccat taagtcgtt cccgggtgg 
      gaaggagta tttaatgcg ggcccactt cgggctgcc 
      cagcgtcga atgcgaaga caccgaagg caaaccgcc 
      aattcaagt caaattcga aattcccta cttcaccac 
      gtcgggaat tcaagtgcg ctatttgaa aattatcga 
      aaagagcgg ctaccaggc cttgccgtg ggtgcgcta 
      cattacgca gaacggcga ctaccaact gcgcaccaa 
      ctgatgaaa actaacatc atgtacgat gattttttg 
      caaatatat tttaaaaca atca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY