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Gene information
General information
TC034520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3834591..3873534
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034520
    iBeetle screen iB_06083

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034520 RA
      MPVGTSKYN QMQRNCYLH PITIFPTEI EAKALELKV 
      LHRDIENHG KIVSSVVKL CKRPDASCN TVNVRRIAN 
      GLERRWHLL FLRSLEWQC YLETLSKKE NCRNTSTSS 
      DETDSDCYD EPVNKYPRL SSRDHSGIE SRTSSENEC 
      GHNQEISNS SLIFQDVMN KNAATLFTP TSEDTVDGK 
      CFRFGEDTS HFTRSNRKA PNLGTYYFK HEDTDSDLE 
      KMEQHSVLS KPEVQSTVE ETSEEEWTY TKNTDEAEV 
      KSKPVPEET IRKLVQKAE ELVSPDKCR KLDRINSIN 
      KMSRVKKWL SMEKPDDSC DASGEDDER ESQISEDFD 
      ESTITCRHN SQNTSFTEL NHSESTPKI NLRQRKYTA 
      NRPWSVSCI SQLDQTSST KIIQNDITN FSISESALH 
      KLAFTSKNL ERADAVSAN SIGVNGNNS TSSTVEETC 
      NLQEKTSPN RRRRLKFKK KCGKIDLHT PYSPIGPKN 
      RNLLIKSGS FSGSSNKFN FTERHTSSD PPAVDLYQG 
      KYETSTTSG ADSEEDLSK RIPSFKLGS KTHYLNSNL 
      RNSSPGKSN TTEEQSSSL SEQAAWDSY QEKYLSEAY 
      SETHDSDAA RKLLEFGED YRNFLDSQS DWSTNPDFS 
      PTFRRRTLV PLSAPESDS DTESLRQLL NSSRDQLNY 
      TKSIYKRQA SLGINEYLV ANEIDEMMS TCDRHFDLL 
      TNVEKKSDE LAISKIDKG IVAELLTQW TNLRNDVAK 
      MQEYRNLQK DISFMKNLL LKMETFDSD CMFNLESVD 
      DLPKEVDYC EKKLEDIYE HKKKLLALN VAVHRFVVE 
      NKEYGGSPL KTDISEMYR MWENLLTNT NERLAQLRP 
      LLQQWKTLD NRLEQLQVD LRSDEKTLH LLDTALQGG 
      VLSDQTASY VRDVAKLLS ETTVVQGCK IPELFTEGG 
      SLSDSGISD EGSEHEIGE RERRLAAIR RLVRQLEVV 
      LAPDCKARL KMAERLNAA EEELKALQK RCRSLIACT 
      AAYSLPVRP CDEQREHEA AKDPDDDDP DEPATNSSW 
      FRRVMRASV PFQVVIITL LCVACLLEP QCCDNMNNF 
      AMSLSPQLR YIKGPPPI
    • >TC034520 RB
      MRFFKLTEE ESDVSVLHR DIENHGKIV SSVVKLCKR 
      PDASCNTVN VRRIANGLE RRWHLLFLR SLEWQCYLE 
      TLSKKENCR NTSTSSDET DSDCYDEPV NKYPRLSSR 
      DHSGIESRT SSENECGHN QEISNSSLI FQDVMNKNA 
      ATLFTPTSE DTVDGKCFR FGEDTSHFT RSNRKAPNL 
      GTYYFKHED TDSDLEKME QHSVLSKPE VQSTVEETS 
      EEEWTYTKN TDEAEVKSK PVPEETIRK LVQKAEELV 
      SPDKCRKLD RINSINKMS RVKKWLSME KPDDSCDAS 
      GEDDERESQ ISEDFDEST ITCRHNSQN TSFTELNHS 
      ESTPKINLR QRKYTANRP WSVSCISQL DQTSSTKII 
      QNDITNFSI SESALHKLA FTSKNLERA DAVSANSIG 
      VNGNNSTSS TVEETCNLQ EKTSPNRRR RLKFKKKCG 
      KIDLHTPYS PIGPKNRNL LIKSGSFSG SSNKFNFTE 
      RHTSSDPPA VDLYQGKYE TSTTSGADS EEDLSKRIP 
      SFKLGSKTH YLNSNLRNS SPGKSNTTE EQSSSLSEQ 
      AAWDSYQEK YLSEAYSET HDSDAARKL LEFGEDYRN 
      FLDSQSDWS TNPDFSPTF RRRTLVPLS APESDSDTE 
      SLRQLLNSS RDQLNYTKS IYKRQASLG INEYLVANE 
      IDEMMSTCD RHFDLLTNV EKKSDELAI SKIDKGIVA 
      ELLTQWTNL RNDVAKMQE YRNLQKDIS FMKNLLLKM 
      ETFDSDCMF NLESVDDLP KEVDYCEKK LEDIYEHKK 
      KLLALNVAV HRFVVENKE YGGSPLKTD ISEMYRMWE 
      NLLTNTNER LAQLRPLLQ QWKTLDNRL EQLQVDLRS 
      DEKTLHLLD TALQGGVLS DQTASYVRD VAKLLSETT 
      VVQGCKIPE LFTEGGSLS DSGISDEGS EHEIGERER 
      RLAAIRRLV RQLEVVLAP DCKARLKMA ERLNAAEEE 
      LKALQKRCR SLIACTAAY SLPVRPCDE QREHEAAKD 
      PDDDDPDEP ATNSSWFRR VMRASVPFQ VVIITLLCV 
      ACLLEPQCC DNMNNFAMS LSPQLRYIK GPPPI
    • >TC034520 RC
      MPVGTTTNL LWKPSVLYS YSVSLLYPL IPIDSRLVF 
      YDTMQPVGD
    CDS sequence
    • >TC034520 RA
      atgcctgtc ggaacgagt aagtacaac cagatgcaa 
      agaaattgt tacctccat cccatcact atatttcct 
      actgaaatt gaagcgaaa gcgttagaa ctaaaggtg 
      ctgcaccgg gatattgaa aatcacggt aaaattgtg 
      agttcagta gtaaagttg tgcaagaga cccgatgca 
      agttgcaac actgtcaac gtccggcga attgcgaac 
      ggcctcgaa aggcgttgg catcttctc ttcttgcga 
      tctttagaa tggcagtgc tacttggaa actttatct 
      aaaaaagaa aattgcagg aatacatcg acctcatct 
      gatgaaact gacagtgac tgttatgac gaaccagtg 
      aataaatat ccgcgattg agcagcagg gatcattct 
      ggcattgaa agcaggaca tcgtctgag aatgaatgt 
      ggccataac caggaaata tcgaactct tccttaata 
      tttcaagac gtaatgaac aagaacgcc gcaacactg 
      tttacccca acaagcgaa gatacagta gatggtaaa 
      tgttttagg tttggcgaa gatacatca catttcaca 
      agatcgaac cgcaaggcc ccgaattta ggaacatac 
      tatttcaag cacgaagac acggactca gatttggaa 
      aaaatggaa cagcattcg gtactgtca aagcccgaa 
      gtccaaagc actgtcgag gaaacatcg gaggaagaa 
      tggacatac acaaaaaac actgacgaa gctgaagtt 
      aaatcgaaa ccagtgccg gaagaaaca atacgaaag 
      ctcgtacaa aaggctgaa gaattagta agtcccgac 
      aagtgccga aaattggac cgaattaat tcgatcaac 
      aaaatgtca agagttaaa aaatggcta agtatggaa 
      aaacctgac gacagttgc gacgctagc ggagaggac 
      gatgaacga gaatctcaa atatccgaa gattttgac 
      gaaagcaca atcacatgc cggcacaac tcgcaaaac 
      acgtccttt acggaactg aaccattct gaatccacg 
      ccgaaaatc aacctgcga caaaggaaa tacactgcc 
      aatcggcct tggagcgtc tcctgcatt tcacaactc 
      gaccagaca tcctcaacg aaaataatt caaaatgat 
      ataacgaac ttttcaata tccgaatct gcgctacac 
      aagcttgcc tttacgtcg aaaaacttg gaaagagcg 
      gacgcagtc agcgccaac agtattggg gttaatggg 
      aacaattca acatcgtca acggttgaa gaaacgtgt 
      aatttgcaa gagaaaaca tccccaaat aggcgaaga 
      cggctcaaa ttcaagaag aagtgtggg aaaatcgat 
      ttgcacacc ccatacagt ccaatcggt ccgaaaaac 
      cgaaacttg ctcataaaa tcaggctca ttctctggc 
      tctagcaac aagtttaac ttcactgaa cgccacact 
      tcgagtgat ccacccgcc gttgatttg tatcaaggg 
      aagtacgaa acttcgacc acaagtggc gctgacagt 
      gaggaggat cttagcaag aggatacca tcgttcaag 
      ttgggttcg aaaacgcac tacctcaat tctaatctt 
      aggaactct agtccgggg aaaagcaac acgaccgag 
      gaacagagc agctctttg tcggagcag gcagcttgg 
      gacagctac caggagaag tatttatct gaggcgtat 
      agcgagacg cacgattcg gacgcggct cgaaaattg 
      ctggagttt ggcgaagat tatcggaat tttctggac 
      agtcagtcc gactggtcg acgaatccc gacttttcg 
      ccgaccttc cgacgccga acgttggtg cctttgtcg 
      gcgcctgag tccgacagc gacacggaa tcgctccgg 
      cagctgctg aacagctca cgagaccaa ctaaactac 
      actaaaagt atttacaag agacaggcg tcgttgggt 
      attaacgaa tacttggta gctaacgaa attgacgaa 
      atgatgtcg acgtgcgac cgtcatttc gacttgcta 
      acaaacgtg gagaagaaa tcggacgaa cttgccatc 
      tcgaagatt gataagggg attgtcgca gaattattg 
      acccagtgg acgaattta cgaaacgac gtcgccaag 
      atgcaagag taccgaaat ttgcaaaaa gatatctcg 
      tttatgaaa aatttgctt ttgaagatg gaaactttc 
      gactctgat tgcatgttc aacttggag tcggtcgac 
      gatcttccc aaagaagtg gattattgc gagaaaaaa 
      ttggaggac atctatgag cacaagaag aagcttttg 
      gcgctgaac gtggccgtt catcgcttc gtggtggaa 
      aataaggag tacggtggg tcgcctttg aagacggac 
      atctcggaa atgtaccgc atgtgggag aatttgcta 
      acaaacact aacgaacgt ttagctcaa ctcagaccc 
      ttgctccaa cagtggaaa acgctcgac aatagactc 
      gagcagtta caagtggat ttacggagc gatgagaaa 
      acattgcat cttcttgat accgctttg cagggcggt 
      gttctatct gatcaaacg gcgagctac gttcgagat 
      gtcgccaaa ttgctctcc gaaaccacc gtcgtacag 
      ggttgcaag attccggag ctgttcacg gaaggaggc 
      tcgctgtcg gacagcggc atttcggac gagggttcg 
      gagcacgag atcggggag cgtgagcgt cggctggcg 
      gctatcaga cgcctggtg cgtcaactg gaagtcgtc 
      ctggccccg gactgcaaa gctcgcctg aaaatggcg 
      gagagactg aacgccgct gaggaagaa ctcaaagct 
      ttgcagaaa cgttgtcga tcgctgatc gcttgcacc 
      gctgcctat tctctgcct gttcggccg tgtgatgaa 
      cagagagaa cacgaagcg gctaaggat cccgacgac 
      gacgatccc gacgagcct gcgacgaat tcctcgtgg 
      tttaggcgc gtaatgcgc gcctcagtt cccttccaa 
      gtggtgatc atcaccctc ttgtgcgtc gcctgcctg 
      ctggagccg cagtgctgc gacaacatg aataatttt 
      gcaatgtcg ctgagcccg caattgcgc tacatcaag 
      ggacccccg ccgatttga
    • >TC034520 RB
      atgagattt tttaagttg accgaggag gaatcggat 
      gtttcagtg ctgcaccgg gatattgaa aatcacggt 
      aaaattgtg agttcagta gtaaagttg tgcaagaga 
      cccgatgca agttgcaac actgtcaac gtccggcga 
      attgcgaac ggcctcgaa aggcgttgg catcttctc 
      ttcttgcga tctttagaa tggcagtgc tacttggaa 
      actttatct aaaaaagaa aattgcagg aatacatcg 
      acctcatct gatgaaact gacagtgac tgttatgac 
      gaaccagtg aataaatat ccgcgattg agcagcagg 
      gatcattct ggcattgaa agcaggaca tcgtctgag 
      aatgaatgt ggccataac caggaaata tcgaactct 
      tccttaata tttcaagac gtaatgaac aagaacgcc 
      gcaacactg tttacccca acaagcgaa gatacagta 
      gatggtaaa tgttttagg tttggcgaa gatacatca 
      catttcaca agatcgaac cgcaaggcc ccgaattta 
      ggaacatac tatttcaag cacgaagac acggactca 
      gatttggaa aaaatggaa cagcattcg gtactgtca 
      aagcccgaa gtccaaagc actgtcgag gaaacatcg 
      gaggaagaa tggacatac acaaaaaac actgacgaa 
      gctgaagtt aaatcgaaa ccagtgccg gaagaaaca 
      atacgaaag ctcgtacaa aaggctgaa gaattagta 
      agtcccgac aagtgccga aaattggac cgaattaat 
      tcgatcaac aaaatgtca agagttaaa aaatggcta 
      agtatggaa aaacctgac gacagttgc gacgctagc 
      ggagaggac gatgaacga gaatctcaa atatccgaa 
      gattttgac gaaagcaca atcacatgc cggcacaac 
      tcgcaaaac acgtccttt acggaactg aaccattct 
      gaatccacg ccgaaaatc aacctgcga caaaggaaa 
      tacactgcc aatcggcct tggagcgtc tcctgcatt 
      tcacaactc gaccagaca tcctcaacg aaaataatt 
      caaaatgat ataacgaac ttttcaata tccgaatct 
      gcgctacac aagcttgcc tttacgtcg aaaaacttg 
      gaaagagcg gacgcagtc agcgccaac agtattggg 
      gttaatggg aacaattca acatcgtca acggttgaa 
      gaaacgtgt aatttgcaa gagaaaaca tccccaaat 
      aggcgaaga cggctcaaa ttcaagaag aagtgtggg 
      aaaatcgat ttgcacacc ccatacagt ccaatcggt 
      ccgaaaaac cgaaacttg ctcataaaa tcaggctca 
      ttctctggc tctagcaac aagtttaac ttcactgaa 
      cgccacact tcgagtgat ccacccgcc gttgatttg 
      tatcaaggg aagtacgaa acttcgacc acaagtggc 
      gctgacagt gaggaggat cttagcaag aggatacca 
      tcgttcaag ttgggttcg aaaacgcac tacctcaat 
      tctaatctt aggaactct agtccgggg aaaagcaac 
      acgaccgag gaacagagc agctctttg tcggagcag 
      gcagcttgg gacagctac caggagaag tatttatct 
      gaggcgtat agcgagacg cacgattcg gacgcggct 
      cgaaaattg ctggagttt ggcgaagat tatcggaat 
      tttctggac agtcagtcc gactggtcg acgaatccc 
      gacttttcg ccgaccttc cgacgccga acgttggtg 
      cctttgtcg gcgcctgag tccgacagc gacacggaa 
      tcgctccgg cagctgctg aacagctca cgagaccaa 
      ctaaactac actaaaagt atttacaag agacaggcg 
      tcgttgggt attaacgaa tacttggta gctaacgaa 
      attgacgaa atgatgtcg acgtgcgac cgtcatttc 
      gacttgcta acaaacgtg gagaagaaa tcggacgaa 
      cttgccatc tcgaagatt gataagggg attgtcgca 
      gaattattg acccagtgg acgaattta cgaaacgac 
      gtcgccaag atgcaagag taccgaaat ttgcaaaaa 
      gatatctcg tttatgaaa aatttgctt ttgaagatg 
      gaaactttc gactctgat tgcatgttc aacttggag 
      tcggtcgac gatcttccc aaagaagtg gattattgc 
      gagaaaaaa ttggaggac atctatgag cacaagaag 
      aagcttttg gcgctgaac gtggccgtt catcgcttc 
      gtggtggaa aataaggag tacggtggg tcgcctttg 
      aagacggac atctcggaa atgtaccgc atgtgggag 
      aatttgcta acaaacact aacgaacgt ttagctcaa 
      ctcagaccc ttgctccaa cagtggaaa acgctcgac 
      aatagactc gagcagtta caagtggat ttacggagc 
      gatgagaaa acattgcat cttcttgat accgctttg 
      cagggcggt gttctatct gatcaaacg gcgagctac 
      gttcgagat gtcgccaaa ttgctctcc gaaaccacc 
      gtcgtacag ggttgcaag attccggag ctgttcacg 
      gaaggaggc tcgctgtcg gacagcggc atttcggac 
      gagggttcg gagcacgag atcggggag cgtgagcgt 
      cggctggcg gctatcaga cgcctggtg cgtcaactg 
      gaagtcgtc ctggccccg gactgcaaa gctcgcctg 
      aaaatggcg gagagactg aacgccgct gaggaagaa 
      ctcaaagct ttgcagaaa cgttgtcga tcgctgatc 
      gcttgcacc gctgcctat tctctgcct gttcggccg 
      tgtgatgaa cagagagaa cacgaagcg gctaaggat 
      cccgacgac gacgatccc gacgagcct gcgacgaat 
      tcctcgtgg tttaggcgc gtaatgcgc gcctcagtt 
      cccttccaa gtggtgatc atcaccctc ttgtgcgtc 
      gcctgcctg ctggagccg cagtgctgc gacaacatg 
      aataatttt gcaatgtcg ctgagcccg caattgcgc 
      tacatcaag ggacccccg ccgatttga
    • >TC034520 RC
      atgcctgtc ggaacgacc accaactta ttgtggaaa 
      ccatctgta ctgtattcg tactctgta agtctgctg 
      tatccactc attccaatc gactctcga ctcgtgttt 
      tacgacaca atgcaacca gttggtgac tga
    mRNA sequence
    • >TC034520 RA
      caaggaaga gaacgaacc gaaacagca gaaagaaca 
      gcagaatgg agtttgaca aggttttgt aaaaagtgt 
      aacgtaaca agttttaaa taattaagt caatagcac 
      gtgcaaaca ctacgctga ggtgcgaag tcaagaaga 
      atctatttt gtgaagtgt cacaggttt cggaataaa 
      gtcatgaac agtgtctac tattacaac ataaccaaa 
      ctttggtct tcagcttca ttattattc ctgtttaac 
      aagtgattc acaagtaca ggtgattcg taattttga 
      tatgttcag tggaacgga gatgcgagg aaatcctag 
      cgtcttgag aacataaaa ctgcctaat gttaaaaaa 
      gcaacaata gccgcaaac atgcctgtc ggaacgagt 
      aagtacaac cagatgcaa agaaattgt tacctccat 
      cccatcact atatttcct actgaaatt gaagcgaaa 
      gcgttagaa ctaaaggtg ctgcaccgg gatattgaa 
      aatcacggt aaaattgtg agttcagta gtaaagttg 
      tgcaagaga cccgatgca agttgcaac actgtcaac 
      gtccggcga attgcgaac ggcctcgaa aggcgttgg 
      catcttctc ttcttgcga tctttagaa tggcagtgc 
      tacttggaa actttatct aaaaaagaa aattgcagg 
      aatacatcg acctcatct gatgaaact gacagtgac 
      tgttatgac gaaccagtg aataaatat ccgcgattg 
      agcagcagg gatcattct ggcattgaa agcaggaca 
      tcgtctgag aatgaatgt ggccataac caggaaata 
      tcgaactct tccttaata tttcaagac gtaatgaac 
      aagaacgcc gcaacactg tttacccca acaagcgaa 
      gatacagta gatggtaaa tgttttagg tttggcgaa 
      gatacatca catttcaca agatcgaac cgcaaggcc 
      ccgaattta ggaacatac tatttcaag cacgaagac 
      acggactca gatttggaa aaaatggaa cagcattcg 
      gtactgtca aagcccgaa gtccaaagc actgtcgag 
      gaaacatcg gaggaagaa tggacatac acaaaaaac 
      actgacgaa gctgaagtt aaatcgaaa ccagtgccg 
      gaagaaaca atacgaaag ctcgtacaa aaggctgaa 
      gaattagta agtcccgac aagtgccga aaattggac 
      cgaattaat tcgatcaac aaaatgtca agagttaaa 
      aaatggcta agtatggaa aaacctgac gacagttgc 
      gacgctagc ggagaggac gatgaacga gaatctcaa 
      atatccgaa gattttgac gaaagcaca atcacatgc 
      cggcacaac tcgcaaaac acgtccttt acggaactg 
      aaccattct gaatccacg ccgaaaatc aacctgcga 
      caaaggaaa tacactgcc aatcggcct tggagcgtc 
      tcctgcatt tcacaactc gaccagaca tcctcaacg 
      aaaataatt caaaatgat ataacgaac ttttcaata 
      tccgaatct gcgctacac aagcttgcc tttacgtcg 
      aaaaacttg gaaagagcg gacgcagtc agcgccaac 
      agtattggg gttaatggg aacaattca acatcgtca 
      acggttgaa gaaacgtgt aatttgcaa gagaaaaca 
      tccccaaat aggcgaaga cggctcaaa ttcaagaag 
      aagtgtggg aaaatcgat ttgcacacc ccatacagt 
      ccaatcggt ccgaaaaac cgaaacttg ctcataaaa 
      tcaggctca ttctctggc tctagcaac aagtttaac 
      ttcactgaa cgccacact tcgagtgat ccacccgcc 
      gttgatttg tatcaaggg aagtacgaa acttcgacc 
      acaagtggc gctgacagt gaggaggat cttagcaag 
      aggatacca tcgttcaag ttgggttcg aaaacgcac 
      tacctcaat tctaatctt aggaactct agtccgggg 
      aaaagcaac acgaccgag gaacagagc agctctttg 
      tcggagcag gcagcttgg gacagctac caggagaag 
      tatttatct gaggcgtat agcgagacg cacgattcg 
      gacgcggct cgaaaattg ctggagttt ggcgaagat 
      tatcggaat tttctggac agtcagtcc gactggtcg 
      acgaatccc gacttttcg ccgaccttc cgacgccga 
      acgttggtg cctttgtcg gcgcctgag tccgacagc 
      gacacggaa tcgctccgg cagctgctg aacagctca 
      cgagaccaa ctaaactac actaaaagt atttacaag 
      agacaggcg tcgttgggt attaacgaa tacttggta 
      gctaacgaa attgacgaa atgatgtcg acgtgcgac 
      cgtcatttc gacttgcta acaaacgtg gagaagaaa 
      tcggacgaa cttgccatc tcgaagatt gataagggg 
      attgtcgca gaattattg acccagtgg acgaattta 
      cgaaacgac gtcgccaag atgcaagag taccgaaat 
      ttgcaaaaa gatatctcg tttatgaaa aatttgctt 
      ttgaagatg gaaactttc gactctgat tgcatgttc 
      aacttggag tcggtcgac gatcttccc aaagaagtg 
      gattattgc gagaaaaaa ttggaggac atctatgag 
      cacaagaag aagcttttg gcgctgaac gtggccgtt 
      catcgcttc gtggtggaa aataaggag tacggtggg 
      tcgcctttg aagacggac atctcggaa atgtaccgc 
      atgtgggag aatttgcta acaaacact aacgaacgt 
      ttagctcaa ctcagaccc ttgctccaa cagtggaaa 
      acgctcgac aatagactc gagcagtta caagtggat 
      ttacggagc gatgagaaa acattgcat cttcttgat 
      accgctttg cagggcggt gttctatct gatcaaacg 
      gcgagctac gttcgagat gtcgccaaa ttgctctcc 
      gaaaccacc gtcgtacag ggttgcaag attccggag 
      ctgttcacg gaaggaggc tcgctgtcg gacagcggc 
      atttcggac gagggttcg gagcacgag atcggggag 
      cgtgagcgt cggctggcg gctatcaga cgcctggtg 
      cgtcaactg gaagtcgtc ctggccccg gactgcaaa 
      gctcgcctg aaaatggcg gagagactg aacgccgct 
      gaggaagaa ctcaaagct ttgcagaaa cgttgtcga 
      tcgctgatc gcttgcacc gctgcctat tctctgcct 
      gttcggccg tgtgatgaa cagagagaa cacgaagcg 
      gctaaggat cccgacgac gacgatccc gacgagcct 
      gcgacgaat tcctcgtgg tttaggcgc gtaatgcgc 
      gcctcagtt cccttccaa gtggtgatc atcaccctc 
      ttgtgcgtc gcctgcctg ctggagccg cagtgctgc 
      gacaacatg aataatttt gcaatgtcg ctgagcccg 
      caattgcgc tacatcaag ggacccccg ccgatttga 
      ggtacttag cgatcttga aacgattga cactgcctt 
      cggtactta tacacttgc acaagacgg gctatttaa 
      gttattgaa ccgacgtag catattgtt attgtgtac 
      atatgattt tttataata aaactgacg gaagtccta 
      tttgcagtt agcaaatga tacgcgaat tcatatcat 
      tttggataa attccagcg agtgttttc tgcctctac 
      tacctattt attattatt attattttg acagtaggg 
      cgaaacatt ttctatggg gagtaaagt attatatac 
      agggtggat gactgaggt catccgcga gtaggtccc 
      gattcggtg cacctgaat attccattg atacgtcac 
      gtgccttaa taaagtttg ctcatattt ttacatcga 
      cgagcacgt aatatttta ttaaactat tgcatgtta 
      gtgttaatt agtatgcat gtgtttatt gattttcgg 
      caacatcgt gtttgcggt tttgtttgg ttcagacat 
      cgatgttgt tgtctaacc gtttggtta gatttattt 
      tatccattt atgattaaa ggttttgcg ttagtaact 
      ggttacgtt actgacact ttaatttta tttgcgaga 
      cgttcggtg atttggttg cgacttttt aagggtgag 
      tcggaaagt tccgacttt atcattgga cgtttcact 
      tatttaata acataaatt atttatgac aatcttggg 
      catttgcgt ttttataag gttatttct tcgatactt 
      tagttttta cagtttcgt agcttgtat cacttatct 
      ataacatac tggatttaa acttaagtt taatattaa 
      gtttatttc atttatccg tcacgtaac tacagtaat 
      agaaacaat tgtcacgca tttgcctat ttttatatt 
      ttttataat actttatat tgcgcatga tgtcataat 
      gtttgcgtt gtaggtctt gattgctta cactgtttt 
      tatgtaaac gaggcgcaa caatcaaaa taaaggatt 
      tttcaaatt
    • >TC034520 RB
      gagttcgca gaaatattt tcttatgat gagattttt 
      taagttgac cgaggagga atcggatgt ttcagtgct 
      gcaccggga tattgaaaa tcacggtaa aattgtgag 
      ttcagtagt aaagttgtg caagagacc cgatgcaag 
      ttgcaacac tgtcaacgt ccggcgaat tgcgaacgg 
      cctcgaaag gcgttggca tcttctctt cttgcgatc 
      tttagaatg gcagtgcta cttggaaac tttatctaa 
      aaaagaaaa ttgcaggaa tacatcgac ctcatctga 
      tgaaactga cagtgactg ttatgacga accagtgaa 
      taaatatcc gcgattgag cagcaggga tcattctgg 
      cattgaaag caggacatc gtctgagaa tgaatgtgg 
      ccataacca ggaaatatc gaactcttc cttaatatt 
      tcaagacgt aatgaacaa gaacgccgc aacactgtt 
      taccccaac aagcgaaga tacagtaga tggtaaatg 
      ttttaggtt tggcgaaga tacatcaca tttcacaag 
      atcgaaccg caaggcccc gaatttagg aacatacta 
      tttcaagca cgaagacac ggactcaga tttggaaaa 
      aatggaaca gcattcggt actgtcaaa gcccgaagt 
      ccaaagcac tgtcgagga aacatcgga ggaagaatg 
      gacatacac aaaaaacac tgacgaagc tgaagttaa 
      atcgaaacc agtgccgga agaaacaat acgaaagct 
      cgtacaaaa ggctgaaga attagtaag tcccgacaa 
      gtgccgaaa attggaccg aattaattc gatcaacaa 
      aatgtcaag agttaaaaa atggctaag tatggaaaa 
      acctgacga cagttgcga cgctagcgg agaggacga 
      tgaacgaga atctcaaat atccgaaga ttttgacga 
      aagcacaat cacatgccg gcacaactc gcaaaacac 
      gtcctttac ggaactgaa ccattctga atccacgcc 
      gaaaatcaa cctgcgaca aaggaaata cactgccaa 
      tcggccttg gagcgtctc ctgcatttc acaactcga 
      ccagacatc ctcaacgaa aataattca aaatgatat 
      aacgaactt ttcaatatc cgaatctgc gctacacaa 
      gcttgcctt tacgtcgaa aaacttgga aagagcgga 
      cgcagtcag cgccaacag tattggggt taatgggaa 
      caattcaac atcgtcaac ggttgaaga aacgtgtaa 
      tttgcaaga gaaaacatc cccaaatag gcgaagacg 
      gctcaaatt caagaagaa gtgtgggaa aatcgattt 
      gcacacccc atacagtcc aatcggtcc gaaaaaccg 
      aaacttgct cataaaatc aggctcatt ctctggctc 
      tagcaacaa gtttaactt cactgaacg ccacacttc 
      gagtgatcc acccgccgt tgatttgta tcaagggaa 
      gtacgaaac ttcgaccac aagtggcgc tgacagtga 
      ggaggatct tagcaagag gataccatc gttcaagtt 
      gggttcgaa aacgcacta cctcaattc taatcttag 
      gaactctag tccggggaa aagcaacac gaccgagga 
      acagagcag ctctttgtc ggagcaggc agcttggga 
      cagctacca ggagaagta tttatctga ggcgtatag 
      cgagacgca cgattcgga cgcggctcg aaaattgct 
      ggagtttgg cgaagatta tcggaattt tctggacag 
      tcagtccga ctggtcgac gaatcccga cttttcgcc 
      gaccttccg acgccgaac gttggtgcc tttgtcggc 
      gcctgagtc cgacagcga cacggaatc gctccggca 
      gctgctgaa cagctcacg agaccaact aaactacac 
      taaaagtat ttacaagag acaggcgtc gttgggtat 
      taacgaata cttggtagc taacgaaat tgacgaaat 
      gatgtcgac gtgcgaccg tcatttcga cttgctaac 
      aaacgtgga gaagaaatc ggacgaact tgccatctc 
      gaagattga taaggggat tgtcgcaga attattgac 
      ccagtggac gaatttacg aaacgacgt cgccaagat 
      gcaagagta ccgaaattt gcaaaaaga tatctcgtt 
      tatgaaaaa tttgctttt gaagatgga aactttcga 
      ctctgattg catgttcaa cttggagtc ggtcgacga 
      tcttcccaa agaagtgga ttattgcga gaaaaaatt 
      ggaggacat ctatgagca caagaagaa gcttttggc 
      gctgaacgt ggccgttca tcgcttcgt ggtggaaaa 
      taaggagta cggtgggtc gcctttgaa gacggacat 
      ctcggaaat gtaccgcat gtgggagaa tttgctaac 
      aaacactaa cgaacgttt agctcaact cagaccctt 
      gctccaaca gtggaaaac gctcgacaa tagactcga 
      gcagttaca agtggattt acggagcga tgagaaaac 
      attgcatct tcttgatac cgctttgca gggcggtgt 
      tctatctga tcaaacggc gagctacgt tcgagatgt 
      cgccaaatt gctctccga aaccaccgt cgtacaggg 
      ttgcaagat tccggagct gttcacgga aggaggctc 
      gctgtcgga cagcggcat ttcggacga gggttcgga 
      gcacgagat cggggagcg tgagcgtcg gctggcggc 
      tatcagacg cctggtgcg tcaactgga agtcgtcct 
      ggccccgga ctgcaaagc tcgcctgaa aatggcgga 
      gagactgaa cgccgctga ggaagaact caaagcttt 
      gcagaaacg ttgtcgatc gctgatcgc ttgcaccgc 
      tgcctattc tctgcctgt tcggccgtg tgatgaaca 
      gagagaaca cgaagcggc taaggatcc cgacgacga 
      cgatcccga cgagcctgc gacgaattc ctcgtggtt 
      taggcgcgt aatgcgcgc ctcagttcc cttccaagt 
      ggtgatcat caccctctt gtgcgtcgc ctgcctgct 
      ggagccgca gtgctgcga caacatgaa taattttgc 
      aatgtcgct gagcccgca attgcgcta catcaaggg 
      acccccgcc gatttgagg tacttagcg atcttgaaa 
      cgattgaca ctgccttcg gtacttata cacttgcac 
      aagacgggc tatttaagt tattgaacc gacgtagca 
      tattgttat tgtgtacat atgattttt tataataaa 
      actgacgga agtcctatt tgcagttag caaatgata 
      cgcgaattc atatcattt tggataaat tccagcgag 
      tgttttctg cctctacta cctatttat tattattat 
      tattttgac agtagggcg aaacatttt ctatgggga 
      gtaaagtat tatatacag ggtggatga ctgaggtca 
      tccgcgagt aggtcccga ttcggtgca cctgaatat 
      tccattgat acgtcacgt gccttaata aagtttgct 
      catattttt acatcgacg agcacgtaa tattttatt 
      aaactattg catgttagt gttaattag tatgcatgt 
      gtttattga ttttcggca acatcgtgt ttgcggttt 
      tgtttggtt cagacatcg atgttgttg tctaaccgt 
      ttggttaga tttatttta tccatttat gattaaagg 
      ttttgcgtt agtaactgg ttacgttac tgacacttt 
      aattttatt tgcgagacg ttcggtgat ttggttgcg 
      actttttaa gggtgagtc ggaaagttc cgactttat 
      cattggacg tttcactta tttaataac ataaattat 
      ttatgacaa tcttgggca tttgcgttt ttataaggt 
      tatttcttc gatacttta gtttttaca gtttcgtag 
      cttgtatca cttatctat aacatactg gatttaaac 
      ttaagttta atattaagt ttatttcat ttatccgtc 
      acgtaacta cagtaatag aaacaattg tcacgcatt 
      tgcctattt ttatatttt ttataatac tttatattg 
      cgcatgatg tcataatgt ttgcgttgt aggtcttga 
      ttgcttaca ctgttttta tgtaaacga ggcgcaaca 
      atcaaaata aaggatttt tcaaatt
    • >TC034520 RC
      caaggaaga gaacgaacc gaaacagca gaaagaaca 
      gcagaatgg agtttgaca aggttttgt aaaaagtgt 
      aacgtaaca agttttaaa taattaagt caatagcac 
      gtgcaaaca ctacgctga ggtgcgaag tcaagaaga 
      atctatttt gtgaagtgt cacaggttt cggaataaa 
      gtcatgaac agtgtctac tattacaac ataaccaaa 
      ctttggtct tcagcttca ttattattc ctgtttaac 
      aagtgattc acaagtaca ggtgattcg taattttga 
      tatgttcag tggaacgga gatgcgagg aaatcctag 
      cgtcttgag aacataaaa ctgcctaat gttaaaaaa 
      gcaacaata gccgcaaac atgcctgtc ggaacgacc 
      accaactta ttgtggaaa ccatctgta ctgtattcg 
      tactctgta agtctgctg tatccactc attccaatc 
      gactctcga ctcgtgttt tacgacaca atgcaacca 
      gttggtgac tgagcttgt ttgtgtgtg cgttgtggc 
      gttttcgaa gtggtgttg ttcaggtgc ttggtgcag 
      gtgcgatat cgtacaggc catggctga aatttgcag 
      atggtactc ttggggact ttgctactg agctctata 
      acgttacac ggctggaaa tataagtta gtacggctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY