Status
Please wait ...
Gene information
General information
TC005190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451085.1: 6593..8455
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005190
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06081

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentiallydegenerated - Number of animals showing the phenotype: 4

    previtellogenic egg chamber structure irregular - Number of animals showing the phenotype: 4

    follicular epithelium sometimes structure irregular - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005190 RA
      MLVLFVTLA LVSAVVASE HPVVTLPNL GQVQGGTKQ 
      SLNGRTFYS FEGVPYAQA PIGENRFKE PVPLTPWKG 
      VWEAKTIYK CMQYNQFTP PGQDYVIGD EDCLYLNIY 
      TPKLDTKAK LDVIVYIHG GAFMFNWAG LQGPEYLLD 
      KDVVLVTLN YRLGPLGFL STEDQVVPG NNGMRDQIF 
      ALQFIKNHV KHFGGNPDS ITIMGMSAG GASTHFHYL 
      SPKSRGLFH RGWSMSGTT LEPWVLMEQ PLAKTRKLA 
      TIVGCSTGK IDEMVTCLK TRPARQIVG AVKEYQPWL 
      YTPFSPFGL VVDSWAADP VLPTHPYQI IKNKQVYDV 
      PWIASYTNS EGLYPAADF YHDEYLQDL DERWNDLVP 
      HLLDYNYTV ERTKMDEVS QKIRKYYLG NKQVTKSTY 
      MDFIPVVSD RIFVIGIQK TARWQAAVT KSPIYSYVF 
      SYRGAHSWS EFCSGGSTE NFGASHGDD TAYIYKYDV 
      VKTATTDQD KKMIKFFVD LLTTYARTG KPKVGVEWP 
      QVPKNLKDE FTFLKIDSP DKLSVGRGI AESTKFWDS 
      LPIKENEKL FADVKDEL
    CDS sequence
    • >TC005190 RA
      atgttggta ctatttgta actcttgcg cttgtttcc 
      gcggtggta gcgtcagaa cacccggtt gtaacttta 
      ccaaacttg gggcaggtc caaggtggt accaaacaa 
      tcgctaaat ggacgcact ttctactcc tttgaagga 
      gtaccatat gcccaggca ccaatcggc gaaaatcgc 
      ttcaaggaa ccggtacca ctcacaccc tggaagggg 
      gtatgggag gccaaaacc atctacaag tgcatgcag 
      tacaaccag ttcacgcca cctggacaa gactacgta 
      attggcgat gaggattgt ctctacttg aacatttac 
      accccaaaa ttggacact aaggccaaa ttggacgtc 
      atagtgtac atccatggg ggcgccttc atgttcaac 
      tgggcgggc ctccaaggg ccggagtac ctcctggac 
      aaggatgta gttttagtg actttaaac taccgtcta 
      ggtccgctc ggcttccta agtaccgaa gaccaggtg 
      gtacctggc aacaacggc atgcgggac caaatcttc 
      gccttgcaa tttatcaaa aaccacgtg aaacacttc 
      ggtgggaac ccagactcc atcaccatc atgggcatg 
      tcagctggt ggggctagt acccacttc cattacctg 
      tcgccaaaa tcgcgcgga ttgttccat cgtggatgg 
      tccatgagt ggtaccacg ctggaacca tgggtactc 
      atggaacaa ccattggcc aagaccagg aaactggcc 
      accattgtt gggtgtagt accgggaaa attgacgaa 
      atggttact tgtttgaaa acaaggcct gctagacag 
      attgtgggg gcggttaaa gagtaccaa ccatggttg 
      tacacaccg ttctcaccc tttggcttg gtggtggat 
      tcatgggct gctgacccg gtactacca acacacccc 
      taccagatt attaagaac aaacaagtg tatgacgtg 
      ccatggatt gcttcgtac acgaattcc gaagggttg 
      tacccagcg gctgatttc taccacgat gagtacctt 
      caagacctt gacgagagg tggaacgac ttggtacca 
      catctcttg gattataat tacacagtt gagaggaca 
      aaaatggac gaagtgagc caaaaaatc cgaaagtac 
      tacctgggc aacaaacaa gtcaccaaa agtacctac 
      atggacttc atccccgtg gtctcagac cggattttt 
      gtaatcggg attcagaaa acagcacga tggcaggcg 
      gctgtgacc aaatcaccc atttattcc tacgttttt 
      agctacaga ggggcccac agttggtcg gagttttgt 
      tctgggggt agtaccgaa aacttcggg gcttcacac 
      ggtgacgac accgcctat atttacaag tacgacgtt 
      gtcaaaacg gcaactact gaccaggac aagaaaatg 
      atcaagttt ttcgttgac ttgctcaca acctatgca 
      cgtaccggg aaacccaaa gttggggtg gagtggcca 
      caagtaccc aaaaatttg aaagatgag ttcactttt 
      ttgaaaatt gattctccg gacaagctt tcagtaggc 
      cgtgggata gccgaaagt accaagttt tgggattcg 
      ttgccaatc aaggagaac gagaagttg tttgcggat 
      gtgaaagat gagttgtaa
    mRNA sequence
    • >TC005190 RA
      attacaagt ccaaaatgt tggtactat ttgtaactc 
      ttgcgcttg tttccgcgg tggtagcgt cagaacacc 
      cggttgtaa ctttaccaa acttggggc aggtccaag 
      gtggtacca aacaatcgc taaatggac gcactttct 
      actcctttg aaggagtac catatgccc aggcaccaa 
      tcggcgaaa atcgcttca aggaaccgg taccactca 
      caccctgga agggggtat gggaggcca aaaccatct 
      acaagtgca tgcagtaca accagttca cgccacctg 
      gacaagact acgtaattg gcgatgagg attgtctct 
      acttgaaca tttacaccc caaaattgg acactaagg 
      ccaaattgg acgtcatag tgtacatcc atgggggcg 
      ccttcatgt tcaactggg cgggcctcc aagggccgg 
      agtacctcc tggacaagg atgtagttt tagtgactt 
      taaactacc gtctaggtc cgctcggct tcctaagta 
      ccgaagacc aggtggtac ctggcaaca acggcatgc 
      gggaccaaa tcttcgcct tgcaattta tcaaaaacc 
      acgtgaaac acttcggtg ggaacccag actccatca 
      ccatcatgg gcatgtcag ctggtgggg ctagtaccc 
      acttccatt acctgtcgc caaaatcgc gcggattgt 
      tccatcgtg gatggtcca tgagtggta ccacgctgg 
      aaccatggg tactcatgg aacaaccat tggccaaga 
      ccaggaaac tggccacca ttgttgggt gtagtaccg 
      ggaaaattg acgaaatgg ttacttgtt tgaaaacaa 
      ggcctgcta gacagattg tgggggcgg ttaaagagt 
      accaaccat ggttgtaca caccgttct caccctttg 
      gcttggtgg tggattcat gggctgctg acccggtac 
      taccaacac acccctacc agattatta agaacaaac 
      aagtgtatg acgtgccat ggattgctt cgtacacga 
      attccgaag ggttgtacc cagcggctg atttctacc 
      acgatgagt accttcaag accttgacg agaggtgga 
      acgacttgg taccacatc tcttggatt ataattaca 
      cagttgaga ggacaaaaa tggacgaag tgagccaaa 
      aaatccgaa agtactacc tgggcaaca aacaagtca 
      ccaaaagta cctacatgg acttcatcc ccgtggtct 
      cagaccgga tttttgtaa tcgggattc agaaaacag 
      cacgatggc aggcggctg tgaccaaat cacccattt 
      attcctacg tttttagct acagagggg cccacagtt 
      ggtcggagt tttgttctg ggggtagta ccgaaaact 
      tcggggctt cacacggtg acgacaccg cctatattt 
      acaagtacg acgttgtca aaacggcaa ctactgacc 
      aggacaaga aaatgatca agtttttcg ttgacttgc 
      tcacaacct atgcacgta ccgggaaac ccaaagttg 
      gggtggagt ggccacaag tacccaaaa atttgaaag 
      atgagttca cttttttga aaattgatt ctccggaca 
      agctttcag taggccgtg ggatagccg aaagtacca 
      agttttggg attcgttgc caatcaagg agaacgaga 
      agttgtttg cggatgtga aagatgagt tgtaatttt 
      taataataa agttgtggc gtcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY