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Gene information
General information
TC004295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451971.1: 59056..62213
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004295
    iBeetle screen iB_06078

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Besides empty eggs, there are a number of various defects known in background such as head irregular, abdominal segments decreased, dorsal closure failed, etc... making up the penetrance of about 30%.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004295 RA
      MAEVASPNK DGKDCGSPS KDKLFDPSV DMLVNDFDD 
ERTLEEEEA LAAGESEDP NAELSSLQK ESNMPLEEL 
LALYGYRGE NQENESPVE EDPDPSEPM EEQESETDP 
PDQPSKLRA LYENIPEND QDAARLLRS VSRVSEEEE 
EDYDYSPDE DEWRKTIMV GSDYQAQIP EGLCHYDDA 
LPYENEDKL LWDPLVINS TVTEDFLKK ASEISKPPI 
PMGSHLRDD EQALYLLQQ CGHNVEEAL RRLRMNVAP 
SSETMSLWS EEECRNFEA GVRCYGKNF HLIQQNKVR 
TRSVGELVQ FYYLWKKSE RHDIFANKT RLEKKKYTL 
HPGLTDFMD RFLEDQDGR DRSSSPNVQ CSVSDNKKS 
VSENATQQT KPDA
    • >TC004295 RB
      MAEVASPNK DGKDCGSPS KDKLFDPSV DMLVNDFDD 
ERTLEEEEA LAAGESEDP NAELSSLQK ESNMPLEEL 
LALYGYRGE NQENESPVE EDPDPSEPM EEQESETDP 
PDQPSKLRA LYENIPEND QDAARLLRS VSRVSEEEE 
EDYDYSPDE DEWRKVNKT IMVGSDYQA QIPEGLCHY 
DDALPYENE DKLLWDPLV INSTVTEDF LKKASEISK 
PPIPMGSHL RDDEQALYL LQQCGHNVE EALRRLRMN 
VAPSSETMS LWSEEECRN FEAGVRCYG KNFHLIQQN 
KVRTRSVGE LVQFYYLWK KSERHDIFA NKTRLEKKK 
YTLHPGLTD FMDRFLEDQ DGRDRSSSP NVQCSVSDN 
KKSVSENAT QQTKPDA
    CDS sequence
    • >TC004295 RA
      atggcagaa gttgcttct ccaaataaa gatggaaaa 
gactgcggc tcacctagc aaagacaag ttatttgac 
ccttccgtt gacatgctt gttaatgac ttcgacgat 
gagcggact ctagaagag gaagaggca ctagcagcg 
ggggaatcc gaagacccc aacgcggag ctctccagc 
ttgcaaaaa gagagtaat atgccattg gaggagttg 
cttgcattg tacggttat cgtggcgaa aatcaggaa 
aacgagagc cccgttgag gaagatccc gacccttct 
gaaccaatg gaagagcaa gagagcgaa actgacccc 
ccagaccaa ccctccaag ctgcgagcc ctctacgag 
aacatcccg gagaatgac caagacgcc gcgcgatta 
ttacgctcc gtttcacgc gttagcgag gaagaagag 
gaagattat gactacagt ccagacgaa gacgagtgg 
cgaaagact attatggtt ggtagcgat taccaagcc 
cagatacct gaaggtttg tgtcattat gacgacgcc 
ttgccgtac gagaacgag gacaagctt ttgtgggat 
cccctcgtt atcaattct accgtgacg gaggatttt 
ttgaaaaaa gcgtccgaa atttccaag cctccgatt 
ccgatgggc agtcatttg cgcgacgac gagcaagcg 
ttgtatttg ttgcaacag tgcgggcac aatgttgag 
gaggcgttg aggcgcttg cgaatgaac gtggcccca 
agcagcgaa acgatgagt ctttggtcg gaggaagag 
tgccgaaat ttcgaggcc ggggtgcga tgttacggg 
aaaaatttc cacctaatt cagcagaat aaggtacgt 
acacggtcg gtgggggag ttagttcag ttctattat 
ttgtggaag aaatcggaa cggcatgac atttttgcg 
aacaaaacc cgactggag aaaaagaaa tacacccta 
catccgggt cttactgac ttcatggat agatttctg 
gaagaccag gatgggagg gataggagc tcgtcccca 
aatgtacag tgcagtgtc tcagacaat aaaaaaagt 
gtctcggaa aatgccaca caacagacg aagccagac 
gcatag
    • >TC004295 RB
      atggcagaa gttgcttct ccaaataaa gatggaaaa 
gactgcggc tcacctagc aaagacaag ttatttgac 
ccttccgtt gacatgctt gttaatgac ttcgacgat 
gagcggact ctagaagag gaagaggca ctagcagcg 
ggggaatcc gaagacccc aacgcggag ctctccagc 
ttgcaaaaa gagagtaat atgccattg gaggagttg 
cttgcattg tacggttat cgtggcgaa aatcaggaa 
aacgagagc cccgttgag gaagatccc gacccttct 
gaaccaatg gaagagcaa gagagcgaa actgacccc 
ccagaccaa ccctccaag ctgcgagcc ctctacgag 
aacatcccg gagaatgac caagacgcc gcgcgatta 
ttacgctcc gtttcacgc gttagcgag gaagaagag 
gaagattat gactacagt ccagacgaa gacgagtgg 
cgaaaggtt aataagact attatggtt ggtagcgat 
taccaagcc cagatacct gaaggtttg tgtcattat 
gacgacgcc ttgccgtac gagaacgag gacaagctt 
ttgtgggat cccctcgtt atcaattct accgtgacg 
gaggatttt ttgaaaaaa gcgtccgaa atttccaag 
cctccgatt ccgatgggc agtcatttg cgcgacgac 
gagcaagcg ttgtatttg ttgcaacag tgcgggcac 
aatgttgag gaggcgttg aggcgcttg cgaatgaac 
gtggcccca agcagcgaa acgatgagt ctttggtcg 
gaggaagag tgccgaaat ttcgaggcc ggggtgcga 
tgttacggg aaaaatttc cacctaatt cagcagaat 
aaggtacgt acacggtcg gtgggggag ttagttcag 
ttctattat ttgtggaag aaatcggaa cggcatgac 
atttttgcg aacaaaacc cgactggag aaaaagaaa 
tacacccta catccgggt cttactgac ttcatggat 
agatttctg gaagaccag gatgggagg gataggagc 
tcgtcccca aatgtacag tgcagtgtc tcagacaat 
aaaaaaagt gtctcggaa aatgccaca caacagacg 
aagccagac gcatag
    mRNA sequence
    • >TC004295 RA
      tatggcgtc tggtcttgg tggtttcgg tggttgtgt 
tttttgcaa aaataaaaa tgttgaagt gaaaaatcg 
gcggaaata gtcaaaaat cggttttaa cgaatgcgt 
gtgcccagt ttggtgtca gttgtggaa gcccaaagt 
aataattaa ttttggtgg aaatcgcgg gctgattcg 
tcaatgtct aacctcact tccaatgtt tttgtttat 
tgtctctgt aactgaacc caatctcta tcgcgggtg 
aatcatgga attattgca ggtttattc acttgcatt 
cgactcgcg ctagcaatt agagtgatg aatgaagag 
taatatcat ggcagaagt tgcttctcc aaataaaga 
tggaaaaga ctgcggctc acctagcaa agacaagtt 
atttgaccc ttccgttga catgcttgt taatgactt 
cgacgatga gcggactct agaagagga agaggcact 
agcagcggg ggaatccga agaccccaa cgcggagct 
ctccagctt gcaaaaaga gagtaatat gccattgga 
ggagttgct tgcattgta cggttatcg tggcgaaaa 
tcaggaaaa cgagagccc cgttgagga agatcccga 
cccttctga accaatgga agagcaaga gagcgaaac 
tgacccccc agaccaacc ctccaagct gcgagccct 
ctacgagaa catcccgga gaatgacca agacgccgc 
gcgattatt acgctccgt ttcacgcgt tagcgagga 
agaagagga agattatga ctacagtcc agacgaaga 
cgagtggcg aaagactat tatggttgg tagcgatta 
ccaagccca gatacctga aggtttgtg tcattatga 
cgacgcctt gccgtacga gaacgagga caagctttt 
gtgggatcc cctcgttat caattctac cgtgacgga 
ggatttttt gaaaaaagc gtccgaaat ttccaagcc 
tccgattcc gatgggcag tcatttgcg cgacgacga 
gcaagcgtt gtatttgtt gcaacagtg cgggcacaa 
tgttgagga ggcgttgag gcgcttgcg aatgaacgt 
ggccccaag cagcgaaac gatgagtct ttggtcgga 
ggaagagtg ccgaaattt cgaggccgg ggtgcgatg 
ttacgggaa aaatttcca cctaattca gcagaataa 
ggtacgtac acggtcggt gggggagtt agttcagtt 
ctattattt gtggaagaa atcggaacg gcatgacat 
ttttgcgaa caaaacccg actggagaa aaagaaata 
caccctaca tccgggtct tactgactt catggatag 
atttctgga agaccagga tgggaggga taggagctc 
gtccccaaa tgtacagtg cagtgtctc agacaataa 
aaaaagtgt ctcggaaaa tgccacaca acagacgaa 
gccagacgc atagtcacg attctaaat taggcttac 
tgcaatttt catttctcc atcatgcaa gctcgattt 
taatgttcc tcttttaca ttcacttca cggaacgca 
aattttatt tatctatat ttttatatt tacatattt 
caccaaaat gagcgaaat tttatttta tacacttat 
acattatgt aaatacgat tttatttat acattactc 
taaaccact ttacagttt tgtgtacag ttttatgcc 
gcgtaattt cattgttcg cgcaagaca tcgcgcctg 
accagtata caacacaca atacatttc aaaatgtga 
atttttggg ttgcattcc cccaagtca ggcgcgtag 
actcacgtg acaacgaaa cataca
    • >TC004295 RB
      tatggcgtc tggtcttgg tggtttcgg tggttgtgt 
tttttgcaa aaataaaaa tgttgaagt gaaaaatcg 
gcggaaata gtcaaaaat cggttttaa cgaatgcgt 
gtgcccagt ttggtgtca gttgtggaa gcccaaagt 
aataattaa ttttggtgg aaatcgcgg gctgattcg 
tcaatgtct aacctcact tccaatgtt tttgtttat 
tgtctctgt aactgaacc caatctcta tcgcgggtg 
aatcatgga attattgca ggtttattc acttgcatt 
cgactcgcg ctagcaatt agagtgatg aatgaagag 
taatatcat ggcagaagt tgcttctcc aaataaaga 
tggaaaaga ctgcggctc acctagcaa agacaagtt 
atttgaccc ttccgttga catgcttgt taatgactt 
cgacgatga gcggactct agaagagga agaggcact 
agcagcggg ggaatccga agaccccaa cgcggagct 
ctccagctt gcaaaaaga gagtaatat gccattgga 
ggagttgct tgcattgta cggttatcg tggcgaaaa 
tcaggaaaa cgagagccc cgttgagga agatcccga 
cccttctga accaatgga agagcaaga gagcgaaac 
tgacccccc agaccaacc ctccaagct gcgagccct 
ctacgagaa catcccgga gaatgacca agacgccgc 
gcgattatt acgctccgt ttcacgcgt tagcgagga 
agaagagga agattatga ctacagtcc agacgaaga 
cgagtggcg aaaggttaa taagactat tatggttgg 
tagcgatta ccaagccca gatacctga aggtttgtg 
tcattatga cgacgcctt gccgtacga gaacgagga 
caagctttt gtgggatcc cctcgttat caattctac 
cgtgacgga ggatttttt gaaaaaagc gtccgaaat 
ttccaagcc tccgattcc gatgggcag tcatttgcg 
cgacgacga gcaagcgtt gtatttgtt gcaacagtg 
cgggcacaa tgttgagga ggcgttgag gcgcttgcg 
aatgaacgt ggccccaag cagcgaaac gatgagtct 
ttggtcgga ggaagagtg ccgaaattt cgaggccgg 
ggtgcgatg ttacgggaa aaatttcca cctaattca 
gcagaataa ggtacgtac acggtcggt gggggagtt 
agttcagtt ctattattt gtggaagaa atcggaacg 
gcatgacat ttttgcgaa caaaacccg actggagaa 
aaagaaata caccctaca tccgggtct tactgactt 
catggatag atttctgga agaccagga tgggaggga 
taggagctc gtccccaaa tgtacagtg cagtgtctc 
agacaataa aaaaagtgt ctcggaaaa tgccacaca 
acagacgaa gccagacgc atagtcacg attctaaat 
taggcttac tgcaatttt catttctcc atcatgcaa 
gctcgattt taatgttcc tcttttaca ttcacttca 
cggaacgca aattttatt tatctatat ttttatatt 
tacatattt caccaaaat gagcgaaat tttatttta 
tacacttat acattatgt aaatacgat tttatttat 
acattactc taaaccact ttacagttt tgtgtacag 
ttttatgcc gcgtaattt cattgttcg cgcaagaca 
tcgcgcctg accagtata caacacaca atacatttc 
aaaatgtga atttttggg ttgcattcc cccaagtca 
ggcgcgtag actcacgtg acaacgaaa cataca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY