| General information | |||
| TC004295 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451971.1: 59056..62213 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004295 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06078 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
| Comment: Besides empty eggs, there are a number of various defects known in background such as head irregular, abdominal segments decreased, dorsal closure failed, etc... making up the penetrance of about 30%. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC004295 RA
MAEVASPNK DGKDCGSPS KDKLFDPSV DMLVNDFDD ERTLEEEEA LAAGESEDP NAELSSLQK ESNMPLEEL LALYGYRGE NQENESPVE EDPDPSEPM EEQESETDP PDQPSKLRA LYENIPEND QDAARLLRS VSRVSEEEE EDYDYSPDE DEWRKTIMV GSDYQAQIP EGLCHYDDA LPYENEDKL LWDPLVINS TVTEDFLKK ASEISKPPI PMGSHLRDD EQALYLLQQ CGHNVEEAL RRLRMNVAP SSETMSLWS EEECRNFEA GVRCYGKNF HLIQQNKVR TRSVGELVQ FYYLWKKSE RHDIFANKT RLEKKKYTL HPGLTDFMD RFLEDQDGR DRSSSPNVQ CSVSDNKKS VSENATQQT KPDA
- >TC004295 RB
MAEVASPNK DGKDCGSPS KDKLFDPSV DMLVNDFDD ERTLEEEEA LAAGESEDP NAELSSLQK ESNMPLEEL LALYGYRGE NQENESPVE EDPDPSEPM EEQESETDP PDQPSKLRA LYENIPEND QDAARLLRS VSRVSEEEE EDYDYSPDE DEWRKVNKT IMVGSDYQA QIPEGLCHY DDALPYENE DKLLWDPLV INSTVTEDF LKKASEISK PPIPMGSHL RDDEQALYL LQQCGHNVE EALRRLRMN VAPSSETMS LWSEEECRN FEAGVRCYG KNFHLIQQN KVRTRSVGE LVQFYYLWK KSERHDIFA NKTRLEKKK YTLHPGLTD FMDRFLEDQ DGRDRSSSP NVQCSVSDN KKSVSENAT QQTKPDA
- >TC004295 RA
atggcagaa gttgcttct ccaaataaa gatggaaaa gactgcggc tcacctagc aaagacaag ttatttgac ccttccgtt gacatgctt gttaatgac ttcgacgat gagcggact ctagaagag gaagaggca ctagcagcg ggggaatcc gaagacccc aacgcggag ctctccagc ttgcaaaaa gagagtaat atgccattg gaggagttg cttgcattg tacggttat cgtggcgaa aatcaggaa aacgagagc cccgttgag gaagatccc gacccttct gaaccaatg gaagagcaa gagagcgaa actgacccc ccagaccaa ccctccaag ctgcgagcc ctctacgag aacatcccg gagaatgac caagacgcc gcgcgatta ttacgctcc gtttcacgc gttagcgag gaagaagag gaagattat gactacagt ccagacgaa gacgagtgg cgaaagact attatggtt ggtagcgat taccaagcc cagatacct gaaggtttg tgtcattat gacgacgcc ttgccgtac gagaacgag gacaagctt ttgtgggat cccctcgtt atcaattct accgtgacg gaggatttt ttgaaaaaa gcgtccgaa atttccaag cctccgatt ccgatgggc agtcatttg cgcgacgac gagcaagcg ttgtatttg ttgcaacag tgcgggcac aatgttgag gaggcgttg aggcgcttg cgaatgaac gtggcccca agcagcgaa acgatgagt ctttggtcg gaggaagag tgccgaaat ttcgaggcc ggggtgcga tgttacggg aaaaatttc cacctaatt cagcagaat aaggtacgt acacggtcg gtgggggag ttagttcag ttctattat ttgtggaag aaatcggaa cggcatgac atttttgcg aacaaaacc cgactggag aaaaagaaa tacacccta catccgggt cttactgac ttcatggat agatttctg gaagaccag gatgggagg gataggagc tcgtcccca aatgtacag tgcagtgtc tcagacaat aaaaaaagt gtctcggaa aatgccaca caacagacg aagccagac gcatag
- >TC004295 RB
atggcagaa gttgcttct ccaaataaa gatggaaaa gactgcggc tcacctagc aaagacaag ttatttgac ccttccgtt gacatgctt gttaatgac ttcgacgat gagcggact ctagaagag gaagaggca ctagcagcg ggggaatcc gaagacccc aacgcggag ctctccagc ttgcaaaaa gagagtaat atgccattg gaggagttg cttgcattg tacggttat cgtggcgaa aatcaggaa aacgagagc cccgttgag gaagatccc gacccttct gaaccaatg gaagagcaa gagagcgaa actgacccc ccagaccaa ccctccaag ctgcgagcc ctctacgag aacatcccg gagaatgac caagacgcc gcgcgatta ttacgctcc gtttcacgc gttagcgag gaagaagag gaagattat gactacagt ccagacgaa gacgagtgg cgaaaggtt aataagact attatggtt ggtagcgat taccaagcc cagatacct gaaggtttg tgtcattat gacgacgcc ttgccgtac gagaacgag gacaagctt ttgtgggat cccctcgtt atcaattct accgtgacg gaggatttt ttgaaaaaa gcgtccgaa atttccaag cctccgatt ccgatgggc agtcatttg cgcgacgac gagcaagcg ttgtatttg ttgcaacag tgcgggcac aatgttgag gaggcgttg aggcgcttg cgaatgaac gtggcccca agcagcgaa acgatgagt ctttggtcg gaggaagag tgccgaaat ttcgaggcc ggggtgcga tgttacggg aaaaatttc cacctaatt cagcagaat aaggtacgt acacggtcg gtgggggag ttagttcag ttctattat ttgtggaag aaatcggaa cggcatgac atttttgcg aacaaaacc cgactggag aaaaagaaa tacacccta catccgggt cttactgac ttcatggat agatttctg gaagaccag gatgggagg gataggagc tcgtcccca aatgtacag tgcagtgtc tcagacaat aaaaaaagt gtctcggaa aatgccaca caacagacg aagccagac gcatag
- >TC004295 RA
tatggcgtc tggtcttgg tggtttcgg tggttgtgt tttttgcaa aaataaaaa tgttgaagt gaaaaatcg gcggaaata gtcaaaaat cggttttaa cgaatgcgt gtgcccagt ttggtgtca gttgtggaa gcccaaagt aataattaa ttttggtgg aaatcgcgg gctgattcg tcaatgtct aacctcact tccaatgtt tttgtttat tgtctctgt aactgaacc caatctcta tcgcgggtg aatcatgga attattgca ggtttattc acttgcatt cgactcgcg ctagcaatt agagtgatg aatgaagag taatatcat ggcagaagt tgcttctcc aaataaaga tggaaaaga ctgcggctc acctagcaa agacaagtt atttgaccc ttccgttga catgcttgt taatgactt cgacgatga gcggactct agaagagga agaggcact agcagcggg ggaatccga agaccccaa cgcggagct ctccagctt gcaaaaaga gagtaatat gccattgga ggagttgct tgcattgta cggttatcg tggcgaaaa tcaggaaaa cgagagccc cgttgagga agatcccga cccttctga accaatgga agagcaaga gagcgaaac tgacccccc agaccaacc ctccaagct gcgagccct ctacgagaa catcccgga gaatgacca agacgccgc gcgattatt acgctccgt ttcacgcgt tagcgagga agaagagga agattatga ctacagtcc agacgaaga cgagtggcg aaagactat tatggttgg tagcgatta ccaagccca gatacctga aggtttgtg tcattatga cgacgcctt gccgtacga gaacgagga caagctttt gtgggatcc cctcgttat caattctac cgtgacgga ggatttttt gaaaaaagc gtccgaaat ttccaagcc tccgattcc gatgggcag tcatttgcg cgacgacga gcaagcgtt gtatttgtt gcaacagtg cgggcacaa tgttgagga ggcgttgag gcgcttgcg aatgaacgt ggccccaag cagcgaaac gatgagtct ttggtcgga ggaagagtg ccgaaattt cgaggccgg ggtgcgatg ttacgggaa aaatttcca cctaattca gcagaataa ggtacgtac acggtcggt gggggagtt agttcagtt ctattattt gtggaagaa atcggaacg gcatgacat ttttgcgaa caaaacccg actggagaa aaagaaata caccctaca tccgggtct tactgactt catggatag atttctgga agaccagga tgggaggga taggagctc gtccccaaa tgtacagtg cagtgtctc agacaataa aaaaagtgt ctcggaaaa tgccacaca acagacgaa gccagacgc atagtcacg attctaaat taggcttac tgcaatttt catttctcc atcatgcaa gctcgattt taatgttcc tcttttaca ttcacttca cggaacgca aattttatt tatctatat ttttatatt tacatattt caccaaaat gagcgaaat tttatttta tacacttat acattatgt aaatacgat tttatttat acattactc taaaccact ttacagttt tgtgtacag ttttatgcc gcgtaattt cattgttcg cgcaagaca tcgcgcctg accagtata caacacaca atacatttc aaaatgtga atttttggg ttgcattcc cccaagtca ggcgcgtag actcacgtg acaacgaaa cataca
- >TC004295 RB
tatggcgtc tggtcttgg tggtttcgg tggttgtgt tttttgcaa aaataaaaa tgttgaagt gaaaaatcg gcggaaata gtcaaaaat cggttttaa cgaatgcgt gtgcccagt ttggtgtca gttgtggaa gcccaaagt aataattaa ttttggtgg aaatcgcgg gctgattcg tcaatgtct aacctcact tccaatgtt tttgtttat tgtctctgt aactgaacc caatctcta tcgcgggtg aatcatgga attattgca ggtttattc acttgcatt cgactcgcg ctagcaatt agagtgatg aatgaagag taatatcat ggcagaagt tgcttctcc aaataaaga tggaaaaga ctgcggctc acctagcaa agacaagtt atttgaccc ttccgttga catgcttgt taatgactt cgacgatga gcggactct agaagagga agaggcact agcagcggg ggaatccga agaccccaa cgcggagct ctccagctt gcaaaaaga gagtaatat gccattgga ggagttgct tgcattgta cggttatcg tggcgaaaa tcaggaaaa cgagagccc cgttgagga agatcccga cccttctga accaatgga agagcaaga gagcgaaac tgacccccc agaccaacc ctccaagct gcgagccct ctacgagaa catcccgga gaatgacca agacgccgc gcgattatt acgctccgt ttcacgcgt tagcgagga agaagagga agattatga ctacagtcc agacgaaga cgagtggcg aaaggttaa taagactat tatggttgg tagcgatta ccaagccca gatacctga aggtttgtg tcattatga cgacgcctt gccgtacga gaacgagga caagctttt gtgggatcc cctcgttat caattctac cgtgacgga ggatttttt gaaaaaagc gtccgaaat ttccaagcc tccgattcc gatgggcag tcatttgcg cgacgacga gcaagcgtt gtatttgtt gcaacagtg cgggcacaa tgttgagga ggcgttgag gcgcttgcg aatgaacgt ggccccaag cagcgaaac gatgagtct ttggtcgga ggaagagtg ccgaaattt cgaggccgg ggtgcgatg ttacgggaa aaatttcca cctaattca gcagaataa ggtacgtac acggtcggt gggggagtt agttcagtt ctattattt gtggaagaa atcggaacg gcatgacat ttttgcgaa caaaacccg actggagaa aaagaaata caccctaca tccgggtct tactgactt catggatag atttctgga agaccagga tgggaggga taggagctc gtccccaaa tgtacagtg cagtgtctc agacaataa aaaaagtgt ctcggaaaa tgccacaca acagacgaa gccagacgc atagtcacg attctaaat taggcttac tgcaatttt catttctcc atcatgcaa gctcgattt taatgttcc tcttttaca ttcacttca cggaacgca aattttatt tatctatat ttttatatt tacatattt caccaaaat gagcgaaat tttatttta tacacttat acattatgt aaatacgat tttatttat acattactc taaaccact ttacagttt tgtgtacag ttttatgcc gcgtaattt cattgttcg cgcaagaca tcgcgcctg accagtata caacacaca atacatttc aaaatgtga atttttggg ttgcattcc cccaagtca ggcgcgtag actcacgtg acaacgaaa cataca
