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Gene information
General information
TC005087 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1812486..1822984
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005087
    iBeetle screen iB_06077

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber early shape irregular - Number of animals showing the phenotype: 4

    tropharium sometimes pattern irregular - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005087 RA
      MNYLGDNAG LGLTSEQTE KVLQLQDLT GIDDMTICR 
      DVLQRHQWN LEVAVQEQL NIREGRPSI YATESRPPA 
      VVSDLLGQH IYYSMPRDG GGGGILGFA KAMFNFFYN 
      ICYSTILAL FQLGRRMIR FEPERPTNP LDDVVGFIN 
      LYQEKYGDC HPVFYQGTF THALNDAKR ELRFLLVYL 
      HKEDHNDAD LFCRESLSH PDVIQYINS RFIFWACSQ 
      SSHEGRRAQ NMIKAGSAP FLGVLVLRD NSMTVVGRM 
      EGFCDPTLL LQRLNTIVS EFEISLVQT RADRYEASL 
      NRSLRAHQD EAFLESLRA DQEKERRRE EERMAREAE 
      LRREEEEAR AEEERRQSI AREKIESVD KVPDEPEKH 
      HPDAVHVVF KLPCGSRIE RRFLKTHSL EAVFYFVFC 
      HPNSPDSFE ITTNFPKRV LKCKPDSST EKIQTLEEA 
      GLKNREVLF VNDLDA
    CDS sequence
    • >TC005087 RA
      atgaattac ctcggagac aacgctggt ttggggctc 
      acgagcgag caaaccgag aaagtatta cagctgcaa 
      gacctcacc ggcatcgac gacatgacg atatgccgc 
      gacgtgctg cagaggcac cagtggaac ctggaggtg 
      gccgtccag gagcagctc aatatcaga gagggcaga 
      ccttcgatt tacgccacg gagagcaga ccgccggcg 
      gttgtgagc gaccttttg gggcaacac atttactat 
      tcgatgcct cgggatggg ggcggtggc ggcattctg 
      ggttttgcc aaagctatg tttaatttt ttctacaat 
      atttgctat agtaccatt ttggccctg tttcaattg 
      gggaggagg atgattcgg ttcgagccc gaaaggccg 
      actaatccg ctagatgat gttgttggg tttattaat 
      ctttaccag gagaagtat ggagattgc catccagtt 
      ttttaccag ggaactttc acgcatgcc cttaatgat 
      gctaaacga gagttacgg ttcctttta gtttatttg 
      cataaagaa gaccacaac gatgctgac ttgttttgc 
      agagagtca ttatcccat ccagacgta atccaatac 
      atcaattcg cgtttcatt ttctgggca tgcagtcaa 
      agcagccac gagggccgt cgcgcccaa aacatgatc 
      aaagcgggc tcagctcca ttcctcgga gtgctagtt 
      ttacgcgac aacagtatg acggtcgtg ggccgtatg 
      gagggtttt tgtgaccct acactcctc ctccaacgt 
      ctcaataca atagtcagc gaatttgaa atcagtctg 
      gtgcaaacc agagccgat cggtacgag gcaagttta 
      aaccgatcg cttagggcg catcaagac gaagcattt 
      ttggagagt ttgagggcc gatcaggag aaggaacga 
      cgcagggag gaggaacgt atggcgagg gaggccgaa 
      ttgaggagg gaggaggaa gaggctaga gctgaggaa 
      gagcgtcga cagagcatc gcaagggag aagattgag 
      tcggtggat aaagtgcct gatgagccc gagaaacat 
      catcccgat gctgtgcat gtagttttt aagttgccg 
      tgtggaagt cgcatcgag cggcgattc ctcaagaca 
      cattccttg gaggccgtt ttttacttc gtattctgc 
      catccgaat tctcccgat tcgtttgaa atcacgacg 
      aattttcct aaaagggtg ctgaagtgc aaacctgac 
      agttcaacc gaaaaaata cagacttta gaagaagcg 
      ggacttaag aatcgagaa gtactattt gtaaatgat 
      ttggacgcc tag
    mRNA sequence
    • >TC005087 RA
      actttcaag acaaagtga cgtttcgaa aataagtgt 
      aatgctcaa taagtgtat caattcacg catttgtaa 
      tttatgcag tgtgacacc gcaccgtat ctaaaaatt 
      ccacgtttt tatgtgcac atgtgatta tcccggccg 
      cgctgtgtt ttcacaatg aattacctc ggagacaac 
      gctggtttg gggctcacg agcgagcaa accgagaaa 
      gtattacag ctgcaagac ctcaccggc atcgacgac 
      atgacgata tgccgcgac gtgctgcag aggcaccag 
      tggaacctg gaggtggcc gtccaggag cagctcaat 
      atcagagag ggcagacct tcgatttac gccacggag 
      agcagaccg ccggcggtt gtgagcgac cttttgggg 
      caacacatt tactattcg atgcctcgg gatgggggc 
      ggtggcggc attctgggt tttgccaaa gctatgttt 
      aattttttc tacaatatt tgctatagt accattttg 
      gccctgttt caattgggg aggaggatg attcggttc 
      gagcccgaa aggccgact aatccgcta gatgatgtt 
      gttgggttt attaatctt taccaggag aagtatgga 
      gattgccat ccagttttt taccaggga actttcacg 
      catgccctt aatgatgct aaacgagag ttacggttc 
      cttttagtt tatttgcat aaagaagac cacaacgat 
      gctgacttg ttttgcaga gagtcatta tcccatcca 
      gacgtaatc caatacatc aattcgcgt ttcattttc 
      tgggcatgc agtcaaagc agccacgag ggccgtcgc 
      gcccaaaac atgatcaaa gcgggctca gctccattc 
      ctcggagtg ctagtttta cgcgacaac agtatgacg 
      gtcgtgggc cgtatggag ggtttttgt gaccctaca 
      ctcctcctc caacgtctc aatacaata gtcagcgaa 
      tttgaaatc agtctggtg caaaccaga gccgatcgg 
      tacgaggca agtttaaac cgatcgctt agggcgcat 
      caagacgaa gcatttttg gagagtttg agggccgat 
      caggagaag gaacgacgc agggaggag gaacgtatg 
      gcgagggag gccgaattg aggagggag gaggaagag 
      gctagagct gaggaagag cgtcgacag agcatcgca 
      agggagaag attgagtcg gtggataaa gtgcctgat 
      gagcccgag aaacatcat cccgatgct gtgcatgta 
      gtttttaag ttgccgtgt ggaagtcgc atcgagcgg 
      cgattcctc aagacacat tccttggag gccgttttt 
      tacttcgta ttctgccat ccgaattct cccgattcg 
      tttgaaatc acgacgaat tttcctaaa agggtgctg 
      aagtgcaaa cctgacagt tcaaccgaa aaaatacag 
      actttagaa gaagcggga cttaagaat cgagaagta 
      ctatttgta aatgatttg gacgcctag tatttatct 
      aaataaaat ttatttatg ttaaaatct acttcgtct 
      aatcataat aagagaatt tttctacag taatttaat 
      tgattacta agtgttgtt tattatttg gattagtca 
      gttgttgaa aaatatgat ttagttttt acatgttga 
      gcatttaaa atagtaact tgtgatttc atttggata 
      agttttgat atgattaaa atgtgcatt tttcattac 
      catacatta tgtaaatat tgtatttac ctaaaaaat 
      aaatctgta attatttaa ttgcctttt attactcaa 
      atgtgccgc gtttggcag agggcgctg ctgacggtt 
      cacgtcaaa gtgacgtca aacgtcaag gtgggtcaa 
      tttgtaatg gcgtcgcgg tgattttca acagtgttt 
      cgacgtctg ttgatacag aaagtcaca aaagtgaaa 
      acaattcga attgaaatg aagaccgcg ctgtgatgt 
      tatccaagt gttgatcaa gatcggaca caaaacgtt 
      gcattgttg tgtacagtg aacatccga aatttacaa 
      aaaatggcc aattttagg aaatcctca agactttta 
      tgtgaatta tttttagtg atattatgt ttagtaaat 
      agggtaagt gtcccacca atttctcat tttacacac 
      tcaagaatc cacctgtca cttcctagt atagagaaa 
      gttggggtc atttttatc tcagcttgt ttttaaatg 
      ttttaattt aaattaaga aaattatca ttgcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY