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Gene information
General information
TC030785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1165957..1167766
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030785
    iBeetle screen iB_06071

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030785 RA
      MSCSTVSVL APNGRRQTV KCTANTTIM QIIEEVCRK 
      QGFKPEDYD IKHHNKVLD TTISFRFSG LPNNAQLEL 
      ATATKARTD SEVILAINS ESGDRVMGN FQPDDTLWS 
      VLTKLCPSI KPDDNPVLV YMRNEIYGD NLQKATLRS 
      LGLRGGRAM MRLMYRRPD ELKVQANVS APLPTKPVE 
      EKPFHRTLK TEAEYSKTT DVKPLQKKT EVDLLKLAR 
      EKRKLSSPS KTPETETHS PKTKPKVES DDEKMDVDL 
      PENIANYPK EPVTNPSSS QLEDDFVFL GERKAMLFS 
      LESARSLPA EDLPDDFFE LTIDDAKKI LGDIRRKRN 
      EMDNANLMT SAMRNLEES KKQLRQLRK YKQSVIRVQ 
      FPDRSILQG SFKPSETVK DIKDFVTSF LEDPNSDFH 
      LYTTPPKNV LSDESKLVE IGCVPGALL HLGANGDGS 
      SVCLKAELR DKFTTNSKA SLAASQLRQ KGTRPESPS 
      EEMGQDESM DTLTEANAA GPSTQPTSY ERKVIKSTE 
      KVPKWFRPS K
    CDS sequence
    • >TC030785 RA
      atgtcgtgt agcaccgtt tcagtcttg gccccaaac 
      ggccgtcga cagaccgtc aaatgtacc gcaaacacg 
      accataatg cagattatt gaagaagtt tgtcgcaaa 
      cagggtttc aagccggag gattacgat attaagcac 
      cacaacaaa gtgcttgac acaactatt tcgttccgt 
      ttttctggt ttgccaaat aacgctcag ttagaatta 
      gcaacagca accaaagca cgcacggac tcggaagta 
      attctagcc ataaattct gaaagtggc gaccgagtc 
      atgggcaac ttccaacca gatgacact ttatggagt 
      gtactaact aaattgtgc cccagtatt aaacctgat 
      gacaatcct gttttggtt tatatgcga aatgaaatt 
      tacggcgac aatttgcaa aaagccact ttgcgaagc 
      ttgggccta agaggtggt agggcgatg atgcggtta 
      atgtatcga aggcctgac gaactaaaa gtccaagca 
      aatgtctct gctcctctg cctacgaaa ccggtagag 
      gagaagccc tttcatcgc actttaaag actgaagct 
      gaatattct aaaactacc gatgtgaag cctttacaa 
      aagaaaact gaggttgat ttattgaaa ctagcacgg 
      gaaaaacga aaattgagc agcccttcc aaaactccg 
      gaaacggaa acgcattcg cctaaaact aaacccaaa 
      gttgagtct gacgacgaa aaaatggat gttgatttg 
      ccagaaaac atcgcaaat tacccaaaa gagccagtt 
      accaaccca tcatctagt cagcttgag gacgatttt 
      gtttttttg ggcgagcgc aaggcaatg ttattttct 
      ctcgaatcg gcgcgttct ttaccagct gaagaccta 
      ccagacgat ttttttgag ctcacaatt gatgatgct 
      aagaaaatt ctgggcgat attagaaga aagcgaaac 
      gaaatggac aacgctaat ctcatgacc tctgcaatg 
      cgcaattta gaagaatca aagaagcaa ttacgccaa 
      ctgcgtaaa tataaacag tcagtaata cgcgtccag 
      ttcccagac aggtccatc cttcaaggg agcttcaag 
      cccagcgaa actgttaaa gatattaaa gatttcgtc 
      acttctttc ctagaagat ccaaacagt gattttcat 
      ctttataca acgccgcct aaaaatgtt ctctcggac 
      gaatcaaaa ctagtggaa attggttgc gtgccgggg 
      gctttgttg catttagga gcaaatggt gatgggagt 
      tctgtgtgc ttgaaggcg gaattgagg gataaattt 
      acaacaaac tcaaaagca agtttagcg gcgtcacag 
      ttgcggcaa aagggaaca agacccgaa tctccgagt 
      gaagaaatg ggccaggat gagtctatg gatacactg 
      actgaagca aatgctgct gggccaagt acacaacca 
      accagttac gagaggaaa gtaataaaa tcaacggaa 
      aaagtccct aagtggttt agaccatca aagtga
    mRNA sequence
    • >TC030785 RA
      gtataaaaa taatcactt attcaaaat gtcgtgtag 
      caccgtttc agtcttggc cccaaacgg ccgtcgaca 
      gaccgtcaa atgtaccgc aaacacgac cataatgca 
      gattattga agaagtttg tcgcaaaca gggtttcaa 
      gccggagga ttacgatat taagcacca caacaaagt 
      gcttgacac aactatttc gttccgttt ttctggttt 
      gccaaataa cgctcagtt agaattagc aacagcaac 
      caaagcacg cacggactc ggaagtaat tctagccat 
      aaattctga aagtggcga ccgagtcat gggcaactt 
      ccaaccaga tgacacttt atggagtgt actaactaa 
      attgtgccc cagtattaa acctgatga caatcctgt 
      tttggttta tatgcgaaa tgaaattta cggcgacaa 
      tttgcaaaa agccacttt gcgaagctt gggcctaag 
      aggtggtag ggcgatgat gcggttaat gtatcgaag 
      gcctgacga actaaaagt ccaagcaaa tgtctctgc 
      tcctctgcc tacgaaacc ggtagagga gaagccctt 
      tcatcgcac tttaaagac tgaagctga atattctaa 
      aactaccga tgtgaagcc tttacaaaa gaaaactga 
      ggttgattt attgaaact agcacggga aaaacgaaa 
      attgagcag cccttccaa aactccgga aacggaaac 
      gcattcgcc taaaactaa acccaaagt tgagtctga 
      cgacgaaaa aatggatgt tgatttgcc agaaaacat 
      cgcaaatta cccaaaaga gccagttac caacccatc 
      atctagtca gcttgagga cgattttgt ttttttggg 
      cgagcgcaa ggcaatgtt attttctct cgaatcggc 
      gcgttcttt accagctga agacctacc agacgattt 
      ttttgagct cacaattga tgatgctaa gaaaattct 
      gggcgatat tagaagaaa gcgaaacga aatggacaa 
      cgctaatct catgacctc tgcaatgcg caatttaga 
      agaatcaaa gaagcaatt acgccaact gcgtaaata 
      taaacagtc agtaatacg cgtccagtt cccagacag 
      gtccatcct tcaagggag cttcaagcc cagcgaaac 
      tgttaaaga tattaaaga tttcgtcac ttctttcct 
      agaagatcc aaacagtga ttttcatct ttatacaac 
      gccgcctaa aaatgttct ctcggacga atcaaaact 
      agtggaaat tggttgcgt gccgggggc tttgttgca 
      tttaggagc aaatggtga tgggagttc tgtgtgctt 
      gaaggcgga attgaggga taaatttac aacaaactc 
      aaaagcaag tttagcggc gtcacagtt gcggcaaaa 
      gggaacaag acccgaatc tccgagtga agaaatggg 
      ccaggatga gtctatgga tacactgac tgaagcaaa 
      tgctgctgg gccaagtac acaaccaac cagttacga 
      gaggaaagt aataaaatc aacggaaaa agtccctaa 
      gtggtttag accatcaaa gtgatttat tagtgttaa 
      gtttatatg agttggtac ttatattaa ataaaagca 
      cttcagcat c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY