Status
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Gene information
General information
TC030784 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18640339..18654498
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030784
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06070

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030784 RA
      MLKNVSRAL LREGRKVYK HKLHSKPNF TKNNYDVVR 
      GNYNYVEEE HLNYFRSLL GESRIFTDL SDLEKYNVD 
      WNRYLRGAS QIVLKPKTT EEVSKILTF CNHHRLAVS 
      PQGGHTGMV GGATPVFDE VIISTELMN EIISLDEKA 
      GILTCLAGC VLQNLDEYL AERNLIFPL DLGAKGSCQ 
      IGGNVSTNA GGLRVLKYG NLHGNVLGL EAVQANGVV 
      LDFLTCHKK DNTGYHLKH LFIGSEGTL GVVTKVAVQ 
      CKLRPKSLH VAFLGLQSF DKVLQTFYK AKQDMDEIL 
      TAFEVIDAP SMDLVTSKL ELESPIGDY PFYVLIETT 
      GSNEEHDEE KVNQFLESC LKKHHILNG TVTGEISKA 
      RGIWQLREK IPEAFKYDG YVFMYDISL PLDHYYKLV 
      DDMREYMGD KSHRVYGFG HIGDGNIHL QISVKEFSP 
      EMKSFIEPY IFHQTKKYN GSISAEHGM GFLKAKYLH 
      MMKTPETIN LMKNVKQMM DPNGILNPY KVLV
    • >TC030784 RB
      MVGGATPVF DEVIISTEL MNEIISLDE KAGILTCLA 
      GCVLQNLDE YLAERNLIF PLDLGAKGS CQIGGNVST 
      NAGGLRVLK YGNLHGNVL GLEAVQANG VVLDFLTCH 
      KKDNTGYHL KHLFIGSEG TLGVVTKVA VQCKLRPKS 
      LHVAFLGLQ SFDKVLQTF YKAKQDMDE ILTAFEVID 
      APSMDLVTS KLELESPIG DYPFYVLIE TTGSNEEHD 
      EEKVNQFLE SCLKKHHIL NGTVTGEIS KARGIWQLR 
      EKIPEAFKY DGYVFMYDI SLPLDHYYK LVDDMREYM 
      GDKSHRVYG FGHIGDGNI HLQISVKEF SPEMKSFIE 
      PYIFHQTKK YNGSISAEH GMGFLKAKY LHMMKTPET 
      INLMKNVKQ MMDPNGILN PYKVLV
    CDS sequence
    • >TC030784 RA
      atgttaaaa aatgtgtca agggcctta ttacgtgaa 
      ggaagaaaa gtatacaaa cataaacta cattccaaa 
      cctaacttt actaagaat aattacgat gttgtaaga 
      ggaaattat aattacgtc gaagaagag cacttaaat 
      tattttcga agccttctc ggggagtct cgaattttc 
      acagatttg tcagatctc gagaaatac aatgtggac 
      tggaacaga tatttaaga ggagcgagt caaattgtt 
      ttaaaacca aaaacgacc gaagaagtc tcaaaaata 
      ttgacattc tgtaaccac catcgcctg gcagtgagc 
      ccccagggg ggccacacg ggaatggtg ggaggggca 
      accccagtt ttcgacgaa gtgattatt tcgaccgaa 
      ctcatgaac gaaattata agtctcgat gaaaaagcc 
      ggtatttta acgtgccta gccggttgt gttttacaa 
      aacttggac gaatattta gctgagcga aatttgatt 
      tttccgctt gatttagga gccaagggc tcttgtcag 
      atcgggggc aacgtctcg acaaatgct ggcggcttg 
      agagtcctc aagtacggc aatctgcac ggaaatgtg 
      ctaggttta gaggcggtg caagccaat ggagttgtt 
      ctagatttt ctaacatgt cacaaaaag gacaacact 
      ggctatcac ctcaaacac ttatttatt ggctccgag 
      gggaccttg ggtgttgtg accaaagta gcagttcag 
      tgtaaattg cggccaaaa tcgctccat gttgcgttc 
      cttggcttg caaagtttt gataaagtg ttgcaaact 
      ttctacaag gctaagcaa gacatggat gagattctc 
      acagctttt gaagtgatt gatgcgcca agtatggat 
      ttggtgacg agtaaactg gagctggag tctccaatc 
      ggggattac ccgttttat gtgttgatt gaaacgacg 
      ggaagcaat gaggaacat gatgaagaa aaagtgaac 
      cagtttttg gagagctgt ttgaagaaa caccacatc 
      ttgaatgga actgtgacc ggggagatt agtaaagca 
      agggggatt tggcaattg agggaaaaa attccagaa 
      gcttttaag tacgatggg tatgttttt atgtacgat 
      atttcgctc cctttggat cattactac aaactagtg 
      gacgatatg agggagtat atgggggat aagtcgcat 
      cgagtttac ggttttgga cacataggc gatggcaac 
      atccacttg caaatctct gtaaaagag ttcagtcct 
      gaaatgaag agtttcatc gaaccatac atcttccat 
      caaacaaaa aaatacaac ggaagcatc agtgcggaa 
      cacgggatg ggtttcctc aaggcaaag tatctacac 
      atgatgaaa acccccgag actatcaat ttgatgaaa 
      aatgtgaaa cagatgatg gatccaaat ggcatactc 
      aatccttac aaggtctta gtgtaa
    • >TC030784 RB
      atggtggga ggggcaacc ccagttttc gacgaagtg 
      attatttcg accgaactc atgaacgaa attataagt 
      ctcgatgaa aaagccggt attttaacg tgcctagcc 
      ggttgtgtt ttacaaaac ttggacgaa tatttagct 
      gagcgaaat ttgattttt ccgcttgat ttaggagcc 
      aagggctct tgtcagatc gggggcaac gtctcgaca 
      aatgctggc ggcttgaga gtcctcaag tacggcaat 
      ctgcacgga aatgtgcta ggtttagag gcggtgcaa 
      gccaatgga gttgttcta gattttcta acatgtcac 
      aaaaaggac aacactggc tatcacctc aaacactta 
      tttattggc tccgagggg accttgggt gttgtgacc 
      aaagtagca gttcagtgt aaattgcgg ccaaaatcg 
      ctccatgtt gcgttcctt ggcttgcaa agttttgat 
      aaagtgttg caaactttc tacaaggct aagcaagac 
      atggatgag attctcaca gcttttgaa gtgattgat 
      gcgccaagt atggatttg gtgacgagt aaactggag 
      ctggagtct ccaatcggg gattacccg ttttatgtg 
      ttgattgaa acgacggga agcaatgag gaacatgat 
      gaagaaaaa gtgaaccag tttttggag agctgtttg 
      aagaaacac cacatcttg aatggaact gtgaccggg 
      gagattagt aaagcaagg gggatttgg caattgagg 
      gaaaaaatt ccagaagct tttaagtac gatgggtat 
      gtttttatg tacgatatt tcgctccct ttggatcat 
      tactacaaa ctagtggac gatatgagg gagtatatg 
      ggggataag tcgcatcga gtttacggt tttggacac 
      ataggcgat ggcaacatc cacttgcaa atctctgta 
      aaagagttc agtcctgaa atgaagagt ttcatcgaa 
      ccatacatc ttccatcaa acaaaaaaa tacaacgga 
      agcatcagt gcggaacac gggatgggt ttcctcaag 
      gcaaagtat ctacacatg atgaaaacc cccgagact 
      atcaatttg atgaaaaat gtgaaacag atgatggat 
      ccaaatggc atactcaat ccttacaag gtcttagtg 
      taa
    mRNA sequence
    • >TC030784 RA
      agtatcaat tcttgttgc tttccgaga taaatatta 
      agttaatcc tatgcataa ttatcttat caaacgtga 
      cttttttat cattgttta aaacccagt aggttttca 
      cgtggtgtg tatatattt ggtatttcg tcaaattta 
      gtttttaaa aaagtgtcg aaaaatgtt aaaaaatgt 
      gtcaagggc cttattacg tgaaggaag aaaagtata 
      caaacataa actacattc caaacctaa ctttactaa 
      gaataatta cgatgttgt aagaggaaa ttataatta 
      cgtcgaaga agagcactt aaattattt tcgaagcct 
      tctcgggga gtctcgaat tttcacaga tttgtcaga 
      tctcgagaa atacaatgt ggactggaa cagatattt 
      aagaggagc gagtcaaat tgttttaaa accaaaaac 
      gaccgaaga agtctcaaa aatattgac attctgtaa 
      ccaccatcg cctggcagt gagccccca ggggggcca 
      cacgggaat ggtgggagg ggcaacccc agttttcga 
      cgaagtgat tatttcgac cgaactcat gaacgaaat 
      tataagtct cgatgaaaa agccggtat tttaacgtg 
      cctagccgg ttgtgtttt acaaaactt ggacgaata 
      tttagctga gcgaaattt gatttttcc gcttgattt 
      aggagccaa gggctcttg tcagatcgg gggcaacgt 
      ctcgacaaa tgctggcgg cttgagagt cctcaagta 
      cggcaatct gcacggaaa tgtgctagg tttagaggc 
      ggtgcaagc caatggagt tgttctaga ttttctaac 
      atgtcacaa aaaggacaa cactggcta tcacctcaa 
      acacttatt tattggctc cgaggggac cttgggtgt 
      tgtgaccaa agtagcagt tcagtgtaa attgcggcc 
      aaaatcgct ccatgttgc gttccttgg cttgcaaag 
      ttttgataa agtgttgca aactttcta caaggctaa 
      gcaagacat ggatgagat tctcacagc ttttgaagt 
      gattgatgc gccaagtat ggatttggt gacgagtaa 
      actggagct ggagtctcc aatcgggga ttacccgtt 
      ttatgtgtt gattgaaac gacgggaag caatgagga 
      acatgatga agaaaaagt gaaccagtt tttggagag 
      ctgtttgaa gaaacacca catcttgaa tggaactgt 
      gaccgggga gattagtaa agcaagggg gatttggca 
      attgaggga aaaaattcc agaagcttt taagtacga 
      tgggtatgt ttttatgta cgatatttc gctcccttt 
      ggatcatta ctacaaact agtggacga tatgaggga 
      gtatatggg ggataagtc gcatcgagt ttacggttt 
      tggacacat aggcgatgg caacatcca cttgcaaat 
      ctctgtaaa agagttcag tcctgaaat gaagagttt 
      catcgaacc atacatctt ccatcaaac aaaaaaata 
      caacggaag catcagtgc ggaacacgg gatgggttt 
      cctcaaggc aaagtatct acacatgat gaaaacccc 
      cgagactat caatttgat gaaaaatgt gaaacagat 
      gatggatcc aaatggcat actcaatcc ttacaaggt 
      cttagtgta agaatttat tatcataaa tattaaaac 
      agatgtttg attttttta tcaatttct gacaaaacc 
      acctttacg gaaggagta caattcaca aaaacgact 
      tccgggtcc accatattc ttataggcc aaaataatg 
      ctacaaaaa attg
    • >TC030784 RB
      tatgttaat ttgtcatga tcagacgag ttaaaagcc 
      cttgtatca ttttttcca aaaacgtga tcagttgat 
      catacttta aacagtatc aattcttgt tgctttccg 
      agataaata ttaagttaa tcctatgca taattatct 
      tatcaaacg tgacttttt tatcattgt ttaaaaccc 
      agtaggttt tcacgtggt gtgtatata tttggtatt 
      tcgtcaaat ttagttttt aaaaaagtg tcgaaaaat 
      gttaaaaaa tgtgtcaag ggccttatt acgtgaagg 
      aagaaaagt atacaaaca taaactaca ttccaaacc 
      taactttac taaggtaaa tactgcaat aaatagatt 
      atagataac ggtagcatt taggcaaac atgtctttt 
      agaataatt acgatgttg taagaggaa attataatt 
      acgtcgaag aagagcact taaattatt ttcgaagcc 
      ttctcgggg agtctcgaa ttttcacag atttgtcag 
      atctcgaga aatacaatg tggactgga acagatatt 
      taagaggag cgagtcaaa ttgttttaa aaccaaaaa 
      cgaccgaag aagtctcaa aaatattga cattctgta 
      accaccatc gcctggcag tgagccccc aggggggcc 
      acacgggaa tggtgggag gggcaaccc cagttttcg 
      acgaagtga ttatttcga ccgaactca tgaacgaaa 
      ttataagtc tcgatgaaa aagccggta ttttaacgt 
      gcctagccg gttgtgttt tacaaaact tggacgaat 
      atttagctg agcgaaatt tgatttttc cgcttgatt 
      taggagcca agggctctt gtcagatcg ggggcaacg 
      tctcgacaa atgctggcg gcttgagag tcctcaagt 
      acggcaatc tgcacggaa atgtgctag gtttagagg 
      cggtgcaag ccaatggag ttgttctag attttctaa 
      catgtcaca aaaaggaca acactggct atcacctca 
      aacacttat ttattggct ccgagggga ccttgggtg 
      ttgtgacca aagtagcag ttcagtgta aattgcggc 
      caaaatcgc tccatgttg cgttccttg gcttgcaaa 
      gttttgata aagtgttgc aaactttct acaaggcta 
      agcaagaca tggatgaga ttctcacag cttttgaag 
      tgattgatg cgccaagta tggatttgg tgacgagta 
      aactggagc tggagtctc caatcgggg attacccgt 
      tttatgtgt tgattgaaa cgacgggaa gcaatgagg 
      aacatgatg aagaaaaag tgaaccagt ttttggaga 
      gctgtttga agaaacacc acatcttga atggaactg 
      tgaccgggg agattagta aagcaaggg ggatttggc 
      aattgaggg aaaaaattc cagaagctt ttaagtacg 
      atgggtatg tttttatgt acgatattt cgctccctt 
      tggatcatt actacaaac tagtggacg atatgaggg 
      agtatatgg gggataagt cgcatcgag tttacggtt 
      ttggacaca taggcgatg gcaacatcc acttgcaaa 
      tctctgtaa aagagttca gtcctgaaa tgaagagtt 
      tcatcgaac catacatct tccatcaaa caaaaaaat 
      acaacggaa gcatcagtg cggaacacg ggatgggtt 
      tcctcaagg caaagtatc tacacatga tgaaaaccc 
      ccgagacta tcaatttga tgaaaaatg tgaaacaga 
      tgatggatc caaatggca tactcaatc cttacaagg 
      tcttagtgt aagaattta ttatcataa atattaaaa 
      cagatgttt gattttttt atcaatttc tgacaaaac 
      cacctttac ggaaggagt acaattcac aaaaacgac 
      ttccgggtc caccatatt cttataggc caaaataat 
      gctacaaaa aattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY