Status
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Gene information
General information
TC030780 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2208902..2214566
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030780
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06069

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030780 RA
      MSLHALIVL SNLFLLVHS YKILALFPH PGKSHVDVF 
      LPLTKALAR KGHEVTVIS HFPLKTPQI NYTDVSLGN 
      ETTPLINIL DMDNKARTR WEKWGIIHL LQEFAAYSC 
      KIGFESSQL REFLRHERT FDVIIVEFF NSDCFAGLV 
      QKFKAPVVG ISSCTIMHW TNERFGNPT HPAYIPNNL 
      MEFSDRMTF FQRIENLLS GLFHHFFYN KIIMNTDET 
      LIRKYLGYE TPTLKQIVF NASLLLVNT HFSLNLPRP 
      LVPAVIEVG GIHIDKPKK IPENLEKWI NESAHGVIY 
      FSLGSMIKG HTFPDEKRS EFLKAFGRL PQRVLWKWE 
      NETMSGKPD NVMIQKWMP QLDILCHPN VKAFISHGG 
      LLGTTEAVH CGVPVVVMP QYGDQFTNA RALEANGGG 
      VILHLSEAT EERIYDALK TILDPRFQK QAKELSARF 
      RDRPLPPLE TAIYWVEYV ARHRGAHHM RTAAVDMPL 
      YKYLLLDVI AFLVLVAGL LFALFFYAT RAILRKLFT 
      KRDKQKTN
    • >TC030780 RB
      MCLKFIILF LNCVFLAES YKILALFPH PGKSHVDVF 
      LPLTKGLAL RGHDVTVVS HFPLPTPMP KYTDVRLGD 
      DTTPLVNIL DIDDYQGRR TRKWLEPYL LHQIAQHSC 
      EIGYQSKPL RDFIKRNET FDLIIAEFF NSNCFLGLG 
      HKFKAPLIG ISSSTIMYW MNERFGNPS HPAYIPNNL 
      LDFSDKLSF FERIENLLV GLVHQAFFT YYASRNDEQ 
      IARKYLGQD LPPIENVIM NSSLLLVNT HFSLNLPRA 
      LVPAVVEVG GIHINKIKA LPRGIIYAV KCSMLIQVV 
      VLLKILLSV HSYTILALF PHRGKSHAN VFLTLTQAL 
      AKKGHEVTA ISYFPLKTP LPNYIDITL GNSSSHFID 
      RINLNDYQG LRTERWFIL RTLNNFSLG MCQQDLSSP 
      SLGDFLKSG PKFDVVLTE YFNCDCFLS VLHKFKSPV 
      IGVSSSTVM HWTNEIFAN PTNPAYVPN NLLNFSDRM 
      AFWERVENF VLGLWQTFY YEFFMLPDG EEIAKRFHN 
      FASLRDIVF NSSLLLVNS HFTLNLPRP LVPAVVEIG 
      GVHIVGVEK LPQNLEKWI NESAHGVIY FSLGSMIKG 
      HTFPDEKRS EFLKAFGRL PQRVLWKWE NETMSGKPD 
      NVMIQKWMP QLDILCHPN VKAFISHGG LLGTTEAVH 
      CGVPVVVMP QYGDQFTNA RALEANGGG VILHLSEAT 
      EERIYDALK TILDPRFQK QAKELSARF RDRPLPPLE 
      TAIYWVEYV ARHRGAHHM RTAAVDMPL YKYLLLDVI 
      AFLVLVAGL LFALFFYAT RAILRKLFT KRDKQKTN
    CDS sequence
    • >TC030780 RA
      atgtctctg cacgcgtta atagtgtta tcaaattta 
      ttcctcttg gtccattct tacaaaatc ctggcgttg 
      tttccccat cccgggaaa agtcacgta gacgtcttt 
      cttccttta acgaaagct ctagcaaga aaaggtcac 
      gaagttacc gtgatcagt cacttcccg ttgaaaaca 
      ccacaaatc aactacacg gacgtgagt ctcggcaac 
      gaaaccacc cctttaatt aacatattg gatatggac 
      aataaagcg cggacgaga tgggagaag tggggcatt 
      atacaccta ctgcaggaa ttcgcagcg tattcctgc 
      aaaattgga ttcgaatcg tcgcaattg cgagagttt 
      ctcagacat gagcgcact ttcgacgtg atcattgtc 
      gaatttttc aatagtgat tgtttcgct gggcttgtg 
      cagaaattc aaagcgccg gtggtcggg attagctcc 
      tgcaccatc atgcactgg actaatgag aggttcggg 
      aatcccaca catccggcc tacattccg aataatctc 
      atggagttt agcgaccga atgacgttt tttcagcga 
      attgaaaac ttgttaagc ggactgttt catcatttt 
      ttttacaac aaaatcata atgaacacg gatgaaacg 
      ctgattcgg aaatatctc ggatatgaa acaccaacg 
      ctcaaacaa attgttttt aatgccagt ttattgctg 
      gtaaacaca catttcagt ttaaatctg ccgagaccc 
      ctagttcct gctgtgatc gaagtcggg gggattcac 
      attgataag ccgaaaaaa ataccagaa aacctcgag 
      aaatggata aacgaatcc gcccacgga gttatttac 
      ttcagtctg ggctcgatg atcaaaggg cacacgttt 
      cccgatgag aaaagaagc gaatttttg aaagctttc 
      ggacgtttg ccgcagcga gtcctttgg aaatgggag 
      aacgaaacg atgtcggga aaaccagac aacgtaatg 
      atccagaag tggatgcct caacttgac attctctgc 
      catccgaac gtgaaagct ttcatatct cacggaggg 
      ctcctgggg acgaccgaa gctgtccac tgcggcgtt 
      ccggtggtg gtgatgccg cagtatggc gatcagttc 
      accaatgcc cgagcgctg gaagcgaac ggcggtgga 
      gtgattctg catctgtcc gaagctacg gaagagaga 
      atctacgac gcgctcaaa acgatcctc gatccgcgg 
      tttcagaaa caagcgaag gagctttca gcgcgtttc 
      agggaccgg ccactgccg cccctcgaa accgccatt 
      tattgggtc gaatatgtc gcacgtcac cgaggagcg 
      caccatatg cggactgca gctgtggac atgcctctc 
      tacaaatat ctcctcctc gatgtgatc gctttcctc 
      gtcttggtc gccggcttg ctgttcgcc ctgttcttc 
      tacgccact agagccatt ctcaggaaa ttattcacg 
      aaaagagac aaacaaaaa accaattaa
    • >TC030780 RB
      atgtgctta aaatttata atcttgttc ctgaactgc 
      gttttcttg gccgagtcg tacaaaatt ttggcgctt 
      tttccccac cctgggaaa agccatgtc gacgttttt 
      cttccgctg actaaaggc ctagccttg agagggcac 
      gacgttacg gtcgtcagt catttccct ctgccaact 
      cccatgcct aaatacaca gacgtaaga ctcggagac 
      gacaccact cctttagtg aacatcctt gacattgac 
      gattatcaa ggaagacgt acgagaaaa tggctagag 
      ccgtatctc ttgcaccaa atcgcccaa cattcgtgc 
      gaaatcggg taccaatct aaacccctg cgagatttt 
      attaaacga aatgagact tttgatcta atcattgcg 
      gaatttttc aacagtaat tgtttcctc ggactgggg 
      cacaaattt aaagcaccg ctgattgga atcagctct 
      tccacgatc atgtattgg atgaatgaa aggttcggt 
      aatccctcc catcctgct tacattcca aataatctc 
      ttggacttt tccgacaag ttaagtttt ttcgaaaga 
      attgaaaac ctcctcgtt gggcttgta catcaagcg 
      ttttttacg tactatgcc tcgcggaat gacgaacaa 
      atcgcgagg aaatatcta ggccaggac ttacctccg 
      attgaaaac gtgataatg aacagtagt ttactcctc 
      gtcaacacc cattttagt ctaaacctg cccagagca 
      cttgttcct gcagtagtg gaagttgga gggatacat 
      attaataaa attaaggcc ctgccaagg ggcattatt 
      tatgcagtg aagtgcagc atgttgatc caagtagta 
      gtgttgtta aaaattctc ttgtcggtg cattcctac 
      accatcctc gcacttttt ccccaccgg ggcaagagt 
      cacgccaat gttttcctc accctgacc caagctctg 
      gcaaaaaag ggacacgaa gtgactgca atcagttac 
      tttcctttg aagacacct cttccaaat tatatcgac 
      atcactttg ggaaactcc tcgtctcat tttattgac 
      aggattaat ctaaacgac tatcagggt ctcaggacc 
      gagagatgg tttattctt agaacactg aacaatttc 
      tcgcttggc atgtgtcaa caggatctt agttcgcca 
      tcacttggg gattttctc aaaagtggg ccgaagttc 
      gacgttgtc ttgactgaa tatttcaat tgtgattgc 
      tttttgagc gttttgcat aaatttaaa agcccggtg 
      attggggtc agttcgtcg actgttatg cattggacg 
      aatgagata tttgctaat cccaccaat ccggcttac 
      gttccgaat aatcttctg aatttttcc gaccgaatg 
      gcattttgg gaaagagtc gaaaatttc gtgctgggg 
      ctttggcaa acgttctat tacgagttc tttatgctg 
      ccagatggg gaagaaatc gcgaagcgg tttcataat 
      ttcgcgtcc ctcagagac atagttttt aatagcagc 
      ttgttgctt gtgaatagt catttcacc ttgaatctg 
      ccacgtccg ctagttcct gcggtggtt gaaattggc 
      ggggttcat attgtgggg gtggaaaaa ttaccacaa 
      aacctcgag aaatggata aacgaatcc gcccacgga 
      gttatttac ttcagtctg ggctcgatg atcaaaggg 
      cacacgttt cccgatgag aaaagaagc gaatttttg 
      aaagctttc ggacgtttg ccgcagcga gtcctttgg 
      aaatgggag aacgaaacg atgtcggga aaaccagac 
      aacgtaatg atccagaag tggatgcct caacttgac 
      attctctgc catccgaac gtgaaagct ttcatatct 
      cacggaggg ctcctgggg acgaccgaa gctgtccac 
      tgcggcgtt ccggtggtg gtgatgccg cagtatggc 
      gatcagttc accaatgcc cgagcgctg gaagcgaac 
      ggcggtgga gtgattctg catctgtcc gaagctacg 
      gaagagaga atctacgac gcgctcaaa acgatcctc 
      gatccgcgg tttcagaaa caagcgaag gagctttca 
      gcgcgtttc agggaccgg ccactgccg cccctcgaa 
      accgccatt tattgggtc gaatatgtc gcacgtcac 
      cgaggagcg caccatatg cggactgca gctgtggac 
      atgcctctc tacaaatat ctcctcctc gatgtgatc 
      gctttcctc gtcttggtc gccggcttg ctgttcgcc 
      ctgttcttc tacgccact agagccatt ctcaggaaa 
      ttattcacg aaaagagac aaacaaaaa accaattaa
    mRNA sequence
    • >TC030780 RA
      ttgttaatg aataagcat ttgggatca aatgtgata 
      cgtcagtgt aacgttact cgcagaaat tatgctcta 
      ataaatttc tcgagttcg ataggcaat tgcaaaact 
      agtaaaaac ttatctcaa gttgcatga ttgtctcaa 
      agttgttat cagaaaacc taattcgga atcgctacc 
      tttgatgcc acaaacagt gacccttta tctcaagaa 
      ggcacacaa acagtaaaa ataaatcat tggctcggg 
      tccaaccgt agtcgtttg ttagcagtc gcgcaatat 
      gtctctgca cgcgttaat agtgttatc aaatttatt 
      cctcttggt ccattctta caaaatcct ggcgttgtt 
      tccccatcc cgggaaaag tcacgtaga cgtctttct 
      tcctttaac gaaagctct agcaagaaa aggtcacga 
      agttaccgt gatcagtca cttcccgtt gaaaacacc 
      acaaatcaa ctacacgga cgtgagtct cggcaacga 
      aaccacccc tttaattaa catattgga tatggacaa 
      taaagcgcg gacgagatg ggagaagtg gggcattat 
      acacctact gcaggaatt cgcagcgta ttcctgcaa 
      aattggatt cgaatcgtc gcaattgcg agagtttct 
      cagacatga gcgcacttt cgacgtgat cattgtcga 
      atttttcaa tagtgattg tttcgctgg gcttgtgca 
      gaaattcaa agcgccggt ggtcgggat tagctcctg 
      caccatcat gcactggac taatgagag gttcgggaa 
      tcccacaca tccggccta cattccgaa taatctcat 
      ggagtttag cgaccgaat gacgttttt tcagcgaat 
      tgaaaactt gttaagcgg actgtttca tcatttttt 
      ttacaacaa aatcataat gaacacgga tgaaacgct 
      gattcggaa atatctcgg atatgaaac accaacgct 
      caaacaaat tgtttttaa tgccagttt attgctggt 
      aaacacaca tttcagttt aaatctgcc gagacccct 
      agttcctgc tgtgatcga agtcggggg gattcacat 
      tgataagcc gaaaaaaat accagaaaa cctcgagaa 
      atggataaa cgaatccgc ccacggagt tatttactt 
      cagtctggg ctcgatgat caaagggca cacgtttcc 
      cgatgagaa aagaagcga atttttgaa agctttcgg 
      acgtttgcc gcagcgagt cctttggaa atgggagaa 
      cgaaacgat gtcgggaaa accagacaa cgtaatgat 
      ccagaagtg gatgcctca acttgacat tctctgcca 
      tccgaacgt gaaagcttt catatctca cggagggct 
      cctggggac gaccgaagc tgtccactg cggcgttcc 
      ggtggtggt gatgccgca gtatggcga tcagttcac 
      caatgcccg agcgctgga agcgaacgg cggtggagt 
      gattctgca tctgtccga agctacgga agagagaat 
      ctacgacgc gctcaaaac gatcctcga tccgcggtt 
      tcagaaaca agcgaagga gctttcagc gcgtttcag 
      ggaccggcc actgccgcc cctcgaaac cgccattta 
      ttgggtcga atatgtcgc acgtcaccg aggagcgca 
      ccatatgcg gactgcagc tgtggacat gcctctcta 
      caaatatct cctcctcga tgtgatcgc tttcctcgt 
      cttggtcgc cggcttgct gttcgccct gttcttcta 
      cgccactag agccattct caggaaatt attcacgaa 
      aagagacaa acaaaaaac caattaatc gaaatgttg 
      ttgtgtatg tgaaggctg tgcccatct gttagtctg 
      atgtaggga aataaaaac gtccaattt at
    • >TC030780 RB
      taaatctcc catcaccgt gtactcgcg actagtggc 
      atgtgctta aaatttata atcttgttc ctgaactgc 
      gttttcttg gccgagtcg tacaaaatt ttggcgctt 
      tttccccac cctgggaaa agccatgtc gacgttttt 
      cttccgctg actaaaggc ctagccttg agagggcac 
      gacgttacg gtcgtcagt catttccct ctgccaact 
      cccatgcct aaatacaca gacgtaaga ctcggagac 
      gacaccact cctttagtg aacatcctt gacattgac 
      gattatcaa ggaagacgt acgagaaaa tggctagag 
      ccgtatctc ttgcaccaa atcgcccaa cattcgtgc 
      gaaatcggg taccaatct aaacccctg cgagatttt 
      attaaacga aatgagact tttgatcta atcattgcg 
      gaatttttc aacagtaat tgtttcctc ggactgggg 
      cacaaattt aaagcaccg ctgattgga atcagctct 
      tccacgatc atgtattgg atgaatgaa aggttcggt 
      aatccctcc catcctgct tacattcca aataatctc 
      ttggacttt tccgacaag ttaagtttt ttcgaaaga 
      attgaaaac ctcctcgtt gggcttgta catcaagcg 
      ttttttacg tactatgcc tcgcggaat gacgaacaa 
      atcgcgagg aaatatcta ggccaggac ttacctccg 
      attgaaaac gtgataatg aacagtagt ttactcctc 
      gtcaacacc cattttagt ctaaacctg cccagagca 
      cttgttcct gcagtagtg gaagttgga gggatacat 
      attaataaa attaaggcc ctgccaagg ggcattatt 
      tatgcagtg aagtgcagc atgttgatc caagtagta 
      gtgttgtta aaaattctc ttgtcggtg cattcctac 
      accatcctc gcacttttt ccccaccgg ggcaagagt 
      cacgccaat gttttcctc accctgacc caagctctg 
      gcaaaaaag ggacacgaa gtgactgca atcagttac 
      tttcctttg aagacacct cttccaaat tatatcgac 
      atcactttg ggaaactcc tcgtctcat tttattgac 
      aggattaat ctaaacgac tatcagggt ctcaggacc 
      gagagatgg tttattctt agaacactg aacaatttc 
      tcgcttggc atgtgtcaa caggatctt agttcgcca 
      tcacttggg gattttctc aaaagtggg ccgaagttc 
      gacgttgtc ttgactgaa tatttcaat tgtgattgc 
      tttttgagc gttttgcat aaatttaaa agcccggtg 
      attggggtc agttcgtcg actgttatg cattggacg 
      aatgagata tttgctaat cccaccaat ccggcttac 
      gttccgaat aatcttctg aatttttcc gaccgaatg 
      gcattttgg gaaagagtc gaaaatttc gtgctgggg 
      ctttggcaa acgttctat tacgagttc tttatgctg 
      ccagatggg gaagaaatc gcgaagcgg tttcataat 
      ttcgcgtcc ctcagagac atagttttt aatagcagc 
      ttgttgctt gtgaatagt catttcacc ttgaatctg 
      ccacgtccg ctagttcct gcggtggtt gaaattggc 
      ggggttcat attgtgggg gtggaaaaa ttaccacaa 
      aacctcgag aaatggata aacgaatcc gcccacgga 
      gttatttac ttcagtctg ggctcgatg atcaaaggg 
      cacacgttt cccgatgag aaaagaagc gaatttttg 
      aaagctttc ggacgtttg ccgcagcga gtcctttgg 
      aaatgggag aacgaaacg atgtcggga aaaccagac 
      aacgtaatg atccagaag tggatgcct caacttgac 
      attctctgc catccgaac gtgaaagct ttcatatct 
      cacggaggg ctcctgggg acgaccgaa gctgtccac 
      tgcggcgtt ccggtggtg gtgatgccg cagtatggc 
      gatcagttc accaatgcc cgagcgctg gaagcgaac 
      ggcggtgga gtgattctg catctgtcc gaagctacg 
      gaagagaga atctacgac gcgctcaaa acgatcctc 
      gatccgcgg tttcagaaa caagcgaag gagctttca 
      gcgcgtttc agggaccgg ccactgccg cccctcgaa 
      accgccatt tattgggtc gaatatgtc gcacgtcac 
      cgaggagcg caccatatg cggactgca gctgtggac 
      atgcctctc tacaaatat ctcctcctc gatgtgatc 
      gctttcctc gtcttggtc gccggcttg ctgttcgcc 
      ctgttcttc tacgccact agagccatt ctcaggaaa 
      ttattcacg aaaagagac aaacaaaaa accaattaa 
      tcgaaatgt tgttgtgta tgtgaaggc tgtgcccat 
      ctgttagtc tgatgtagg gaaataaaa acgtccaat 
      ttat
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