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Gene information
General information
TC030775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2437862..2445825
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030775
    iBeetle screen iB_06066

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030775 RA
      MAENVKVIV RCRPMNKKE QDLNCDCVV KMRNCVVET 
      FDPSEGPSF PKQFTFDST YDQDSTTEM IYNDICYPL 
      VESVLEGYN ATIFVYGQT GCGKSHTME GEKSGPEKG 
      VISRAFEHI FEAISVTTG VKYLALISY LEIYNEQIR 
      DLLLPSDKI GNSALNLKE SPTEGVTVP GLTSHPVHN 
      AAECEHFLN LGSKNRIIG ATLMNQNSS RSHSIFTIS 
      IEQITNVNN NESFKKGKL NLVDLAGSE RQAKTGATG 
      DRLKEATKI NLSLSALGN VISALVDGK AKHIPYRDS 
      KLTRLLQDS LGGNTRTLM IACISPSSR DYVETLSTL 
      RYANRAKNI HNKPRVNED PKDTMLRQY QEEIERLKS 
      LLGQQVKIE EVKEIETSP NSDLDAKRD QLIQEYQQE 
      MEKLKNLHE CEKHEKENV LKQIDLIKD EYEKNIEKL 
      NSEIQEKKR KELCSKEEI LNRIEALKN AMIGGEKAN 
      DKELSERRK RKKQAAEKR ASVIAHLLA KIDMNEDRE 
      ILHNQYKDI SQELNLKTE ALRKYRHKV KALEKEISD 
      IQSEFEAER QDYLETIRK QDQHIKLLA QISDKIAST 
      LKKECNYSD LESIKEQAV WLEDLQKYK LPDLITPRT 
      KLPPPGRLH DIQTAPARL QESLDDDSF DNDFLEKKL 
      EKSEKEDII GSYFAPTTK RAAELLNQS SRIDANRVI 
      STWRGSMTF ADFARRNKP KVAEEHEGE RPGRPPNLN 
      SSLNNLWVN EGTPRKPLR LEALPNIDR HRSRNQRLN 
      TLDIL
    CDS sequence
    • >TC030775 RA
      atggctgaa aacgtaaaa gtaatcgta cgatgtcgc 
      cccatgaac aagaaagaa caagacctc aactgcgac 
      tgtgtcgtg aagatgaga aactgtgtg gtcgaaacg 
      ttcgaccct tccgagggt ccttctttc cccaaacag 
      tttactttc gactcaact tacgaccaa gactccacc 
      actgaaatg atctacaac gacatctgc taccccctt 
      gtcgaaagc gtcctcgaa ggttacaat gccacaatt 
      ttcgtctac ggccaaacc ggctgcgga aaatcacac 
      acaatggag ggcgaaaaa tcgggcccc gaaaaaggg 
      gtcatttcc cgggccttc gagcacatc tttgaggcc 
      atttcagtc accactgga gtcaaatat ttagccctt 
      atcagttat cttgaaatt tataacgaa caaatcagg 
      gatttgtta ctcccaagt gataaaatc gggaacagt 
      gctctcaac ttgaaggag agccccact gaaggggtt 
      acagtcccc ggactgacg tcacatcca gtgcacaat 
      gccgcagaa tgtgaacat tttttgaac ctaggctcg 
      aaaaatcgc ataataggg gcgactcta atgaatcaa 
      aatagttcg agatcgcac agtattttc accataagc 
      atcgaacag ataactaac gtgaacaat aatgagagt 
      tttaagaaa gggaagttg aatttggtc gatttggcc 
      ggttctgaa cgacaggca aagactggg gctacgggt 
      gatcggtta aaagaagcg actaaaatt aatctgtct 
      ttgagtgcc ttaggtaat gttatttcg gccctggtg 
      gacggcaaa gccaaacat ataccgtac agggactcg 
      aaattgacc agattgtta caggactct ttgggggga 
      aatactcga acgctgatg atcgcctgc atcagccct 
      tctagtcgt gattacgtt gaaacgcta tcaacactc 
      agatatgca aatagggcc aaaaacatc cacaacaag 
      ccgcgagtt aacgaagac cccaaagac acaatgttg 
      agacaatac caggaagaa atcgaacgc ttaaagagt 
      ctcttaggc caacaagtg aaaattgaa gaagttaaa 
      gaaattgaa acttcgcca aattccgac ctggacgcc 
      aaacgcgac caattgatc caggaatac caacaagaa 
      atggaaaaa ttgaaaaac ctccacgag tgtgaaaaa 
      cacgagaag gaaaacgtc ttgaagcaa atcgacttg 
      atcaaggac gaatacgag aaaaacatt gaaaaacta 
      aacagtgag atacaagag aaaaaacga aaagagttg 
      tgttccaaa gaggaaatt ttgaaccgg attgaagct 
      ttgaaaaat gcaatgatt ggcggggag aaggccaat 
      gataaagaa ctgtcggaa cgaagaaaa aggaagaaa 
      caagcggcg gaaaaacgg gccagtgtt attgcccat 
      cttctcgca aaaatcgac atgaatgaa gatcgggaa 
      atacttcat aatcaatac aaggacatc agtcaggag 
      ctcaatctc aagactgag gctttgcgt aaatataga 
      cacaaagtt aaggcactt gagaaggaa atttcggac 
      attcagagc gagtttgag gccgaaaga caggactat 
      cttgaaaca ataagaaag caagaccaa cacatcaag 
      ctcttggcg caaattagc gacaagatt gcgtccact 
      ttgaagaaa gagtgcaac tacagcgac ttggagagc 
      atcaaagag caggcggtg tggttggag gacctccag 
      aagtacaaa ctccccgac ctgataacc ccacggact 
      aaactaccc cctcccggt cgcttacac gacatacaa 
      acagctccg gccagatta caagaatct ctcgacgac 
      gattcgttc gataacgat tttttggaa aagaaattg 
      gaaaaatcg gagaaggag gacataatc ggaagttat 
      ttcgctccg acgacgaaa cgagcggcg gagttgtta 
      aatcagtcg tcaaggatc gatgccaat cgggttatc 
      agcacgtgg aggggatcg atgacgttt gcagatttc 
      gcgcgacgg aacaagccg aaagtggcc gaagaacac 
      gaaggggag cggcccggg agacccccc aatttgaat 
      tccagccta aataatcta tgggtcaat gaggggact 
      ccccggaag cctctacgg ctggaagcg ttgccaaac 
      atcgataga catcgtagc agaaaccaa agacttaac 
      actttagat attttgtaa
    mRNA sequence
    • >TC030775 RA
      attattaag ccccacatg cggaaagtg ggtcatacc 
      agcagtaca aacacttta aagcgcctc agacaaaaa 
      tttacaatg caaacaacc ccacattta aaaaaaccg 
      tgacgtgat tagcaaaat tcccaagaa aactccgac 
      tcaaatcaa ttttcaccc cgttttcga tccgaattc 
      cggagcgca ggtcgccac ttttacatg gtcagttta 
      gtcgcgacc atcgaccat gatgggtgt accagttgg 
      gctcatttt tgtaccttc gttttttta gtaaacaag 
      tatggcatg atttgattt aaaatggct gaaaacgta 
      aaagtaatc gtacgatgt cgccccatg aacaagaaa 
      gaacaagac ctcaactgc gactgtgtc gtgaagatg 
      agaaactgt gtggtcgaa acgttcgac ccttccgag 
      ggtccttct ttccccaaa cagtttact ttcgactca 
      acttacgac caagactcc accactgaa atgatctac 
      aacgacatc tgctacccc cttgtcgaa agcgtcctc 
      gaaggttac aatgccaca attttcgtc tacggccaa 
      accggctgc ggaaaatca cacacaatg gagggcgaa 
      aaatcgggc cccgaaaaa ggggtcatt tcccgggcc 
      ttcgagcac atctttgag gccatttca gtcaccact 
      ggagtcaaa tatttagcc cttatcagt tatcttgaa 
      atttataac gaacaaatc agggatttg ttactccca 
      agtgataaa atcgggaac agtgctctc aacttgaag 
      gagagcccc actgaaggg gttacagtc cccggactg 
      acgtcacat ccagtgcac aatgccgca gaatgtgaa 
      cattttttg aacctaggc tcgaaaaat cgcataata 
      ggggcgact ctaatgaat caaaatagt tcgagatcg 
      cacagtatt ttcaccata agcatcgaa cagataact 
      aacgtgaac aataatgag agttttaag aaagggaag 
      ttgaatttg gtcgatttg gccggttct gaacgacag 
      gcaaagact ggggctacg ggtgatcgg ttaaaagaa 
      gcgactaaa attaatctg tctttgagt gccttaggt 
      aatgttatt tcggccctg gtggacggc aaagccaaa 
      catataccg tacagggac tcgaaattg accagattg 
      ttacaggac tctttgggg ggaaatact cgaacgctg 
      atgatcgcc tgcatcagc ccttctagt cgtgattac 
      gttgaaacg ctatcaaca ctcagatat gcaaatagg 
      gccaaaaac atccacaac aagccgcga gttaacgaa 
      gaccccaaa gacacaatg ttgagacaa taccaggaa 
      gaaatcgaa cgcttaaag agtctctta ggccaacaa 
      gtgaaaatt gaagaagtt aaagaaatt gaaacttcg 
      ccaaattcc gacctggac gccaaacgc gaccaattg 
      atccaggaa taccaacaa gaaatggaa aaattgaaa 
      aacctccac gagtgtgaa aaacacgag aaggaaaac 
      gtcttgaag caaatcgac ttgatcaag gacgaatac 
      gagaaaaac attgaaaaa ctaaacagt gagatacaa 
      gagaaaaaa cgaaaagag ttgtgttcc aaagaggaa 
      attttgaac cggattgaa gctttgaaa aatgcaatg 
      attggcggg gagaaggcc aatgataaa gaactgtcg 
      gaacgaaga aaaaggaag aaacaagcg gcggaaaaa 
      cgggccagt gttattgcc catcttctc gcaaaaatc 
      gacatgaat gaagatcgg gaaatactt cataatcaa 
      tacaaggac atcagtcag gagctcaat ctcaagact 
      gaggctttg cgtaaatat agacacaaa gttaaggca 
      cttgagaag gaaatttcg gacattcag agcgagttt 
      gaggccgaa agacaggac tatcttgaa acaataaga 
      aagcaagac caacacatc aagctcttg gcgcaaatt 
      agcgacaag attgcgtcc actttgaag aaagagtgc 
      aactacagc gacttggag agcatcaaa gagcaggcg 
      gtgtggttg gaggacctc cagaagtac aaactcccc 
      gacctgata accccacgg actaaacta ccccctccc 
      ggtcgctta cacgacata caaacagct ccggccaga 
      ttacaagaa tctctcgac gacgattcg ttcgataac 
      gattttttg gaaaagaaa ttggaaaaa tcggagaag 
      gaggacata atcggaagt tatttcgct ccgacgacg 
      aaacgagcg gcggagttg ttaaatcag tcgtcaagg 
      atcgatgcc aatcgggtt atcagcacg tggagggga 
      tcgatgacg tttgcagat ttcgcgcga cggaacaag 
      ccgaaagtg gccgaagaa cacgaaggg gagcggccc 
      gggagaccc cccaatttg aattccagc ctaaataat 
      ctatgggtc aatgagggg actccccgg aagcctcta 
      cggctggaa gcgttgcca aacatcgat agacatcgt 
      agcagaaac caaagactt aacacttta gatattttg 
      taaattatt tagagactt aacattgtt gaggtttac 
      acgagtttg gagttacgt tgtgataaa tattgtaaa 
      tttaaacga ccaacatgt gtgatacgt tcataattt 
      ttaacatat tttattatg tatttttat gagaataaa 
      atacatcaa cgagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY