Status
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Gene information
General information
TC030759 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11954208..11956114
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030759
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06063

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030759 RA
      MCGIFCLLS FDKSNTETL HYKFELFKE QISRRGPNS 
      LKTDFCQLN NCNILFASS VLWLQGTDI TTQPCKNDN 
      SVFIYNGDI FGGSQIGQQ LNSGSGDTN LFFDALQNS 
      DDILMTLDN IEGPYAFVY LNVVQNKVY IGRDIFGRR 
      SLLIGRNAN EFVITSVAK RGTEFEFME LPSIGIFSI 
      DLDTKQWKI LPWRRKNKN FVEKLSEFE LFINQTVLK 
      QADVFDRKK TFVPPSEAQ LKLLTGFLD LQTCDLFNF 
      LFNSPFMNN ILRLKELLQ NSIKKRILA QPKFCQNCY 
      KKYDCDHAI TGILFSGGV DCAVLALLA STVIDPLRP 
      IDLMNVAFE KGGCYDTPD RITGLSTLR ELQQLCPDR 
      KWNFVEVNV TREELDKCR RDRISDLIF PLNSILDDS 
      LGCALWFAS RGVTQDYTT PCRVKTEVF HLSHQIQTL 
      LVGMGADEL FGGYTRHRA AFQKKGWEG LSEILDEDW 
      QNLPYRNLA RDDRVVSDH GRQLRTPYL DEEVVKFVH 
      SLHTWDKTF PSIKLQGIG EKLLLRSLA YHLGLTKAA 
      LLKKRALQF GSRIANSKE KGHEISQHL TAT
    CDS sequence
    • >TC030759 RA
      atgtgtgga atattttgt ttattatca ttcgacaaa 
      agcaatact gaaacatta cattacaag tttgaattg 
      ttcaaagaa caaatttcg cgaaggggc cccaactcg 
      ttgaaaacc gacttttgc cagctgaat aattgcaat 
      atcctgttc gcctcgagc gttttgtgg cttcaaggt 
      accgatatt acgacacaa ccgtgcaaa aatgataat 
      tctgtcttt atttacaat ggggatatt ttcgggggt 
      tcccaaatc ggtcaacaa ttaaatagt ggttcaggc 
      gatacgaac ctatttttc gacgctttg caaaattcg 
      gacgatatt ttaatgacg ctagacaac attgagggg 
      ccttacgcg ttcgtgtac ttaaacgtg gtacaaaat 
      aaagtttac attgggcgt gatattttc ggcagacgt 
      agtttacta atcggtaga aatgcaaac gaatttgta 
      ataacaagt gttgcaaaa agggggaca gagtttgag 
      tttatggaa ctgccaagc atcggcata ttttctatt 
      gatcttgat acaaaacag tggaaaatt ttaccgtgg 
      cgtcggaaa aacaaaaat ttcgttgag aaattaagc 
      gaatttgag ttatttatc aatcagact gtgctaaaa 
      caggctgat gtatttgac cgaaagaaa acgttcgtt 
      cctcctagc gaggcccag ctgaaacta ttgactggg 
      tttttggat ttacaaact tgtgatttg tttaatttt 
      ttatttaat tctccattc atgaacaat attttacga 
      ctgaaggaa cttctccaa aatagcata aaaaaacgt 
      attttagcc caaccgaaa ttttgccaa aactgttac 
      aaaaaatac gattgtgat cacgctatt actggaatt 
      ttgttttcg gggggtgtg gattgtgcc gttttggcc 
      cttcttgcg agtacagta atcgaccct ttgcgtcct 
      attgattta atgaacgta gcgtttgaa aaagggggc 
      tgttacgac acccctgat cgcattacg ggccttagc 
      accctcaga gagttgcaa caattgtgt cctgaccgg 
      aaatggaac ttcgtcgaa gttaatgta acgcgcgag 
      gagttagat aagtgtaga cgcgaccga atttccgat 
      ttaattttt ccgttaaat tcgattctg gatgatagt 
      ttaggctgt gcattgtgg tttgctagt cgtggagta 
      acccaggat tacacaacg ccgtgtagg gtaaaaact 
      gaagttttc catttgtca caccaaatt cagactttg 
      ttggtaggg atgggggcc gatgagcta tttgggggg 
      tacacaaga cacagagct gcgtttcag aaaaagggt 
      tgggagggt ttgagtgag attttggac gaggattgg 
      caaaatttg ccttataga aacttggct agggatgac 
      agagtggtg tcagatcat gggagacaa ctccgaacg 
      ccgtatttg gacgaggaa gttgtgaaa tttgtacac 
      agtttacac acttgggac aagactttt ccatcaatt 
      aaattgcaa ggaattggt gaaaaactt cttcttaga 
      agtcttgcg tatcatttg gggcttaca aaggccgcc 
      cttttgaag aaaagggct ctacagttc gggtcaagg 
      attgcgaac tcgaaagaa aagggacac gaaatttcg 
      cagcattta accgctaca taa
    mRNA sequence
    • >TC030759 RA
      ttgttacaa gaacgtcaa aaccttaac ctcaaagtt 
      tgaaatgtg tggaatatt ttgtttatt atcattcga 
      caaaagcaa tactgaaac attacatta caagtttga 
      attgttcaa agaacaaat ttcgcgaag gggccccaa 
      ctcgttgaa aaccgactt ttgccagct gaataattg 
      caatatcct gttcgcctc gagcgtttt gtggcttca 
      aggtaccga tattacgac acaaccgtg caaaaatga 
      taattctgt ctttattta caatgggga tattttcgg 
      gggttccca aatcggtca acaattaaa tagtggttc 
      aggcgatac gaacctatt tttcgacgc tttgcaaaa 
      ttcggacga tattttaat gacgctaga caacattga 
      ggggcctta cgcgttcgt gtacttaaa cgtggtaca 
      aaataaagt ttacattgg gcgtgatat tttcggcag 
      acgtagttt actaatcgg tagaaatgc aaacgaatt 
      tgtaataac aagtgttgc aaaaagggg gacagagtt 
      tgagtttat ggaactgcc aagcatcgg catattttc 
      tattgatct tgatacaaa acagtggaa aattttacc 
      gtggcgtcg gaaaaacaa aaatttcgt tgagaaatt 
      aagcgaatt tgagttatt tatcaatca gactgtgct 
      aaaacaggc tgatgtatt tgaccgaaa gaaaacgtt 
      cgttcctcc tagcgaggc ccagctgaa actattgac 
      tgggttttt ggatttaca aacttgtga tttgtttaa 
      ttttttatt taattctcc attcatgaa caatatttt 
      acgactgaa ggaacttct ccaaaatag cataaaaaa 
      acgtatttt agcccaacc gaaattttg ccaaaactg 
      ttacaaaaa atacgattg tgatcacgc tattactgg 
      aattttgtt ttcgggggg tgtggattg tgccgtttt 
      ggcccttct tgcgagtac agtaatcga ccctttgcg 
      tcctattga tttaatgaa cgtagcgtt tgaaaaagg 
      gggctgtta cgacacccc tgatcgcat tacgggcct 
      tagcaccct cagagagtt gcaacaatt gtgtcctga 
      ccggaaatg gaacttcgt cgaagttaa tgtaacgcg 
      cgaggagtt agataagtg tagacgcga ccgaatttc 
      cgatttaat ttttccgtt aaattcgat tctggatga 
      tagtttagg ctgtgcatt gtggtttgc tagtcgtgg 
      agtaaccca ggattacac aacgccgtg tagggtaaa 
      aactgaagt tttccattt gtcacacca aattcagac 
      tttgttggt agggatggg ggccgatga gctatttgg 
      ggggtacac aagacacag agctgcgtt tcagaaaaa 
      gggttggga gggtttgag tgagatttt ggacgagga 
      ttggcaaaa tttgcctta tagaaactt ggctaggga 
      tgacagagt ggtgtcaga tcatgggag acaactccg 
      aacgccgta tttggacga ggaagttgt gaaatttgt 
      acacagttt acacacttg ggacaagac ttttccatc 
      aattaaatt gcaaggaat tggtgaaaa acttcttct 
      tagaagtct tgcgtatca tttggggct tacaaaggc 
      cgccctttt gaagaaaag ggctctaca gttcgggtc 
      aaggattgc gaactcgaa agaaaaggg acacgaaat 
      ttcgcagca tttaaccgc tacataaaa aaaattgta 
      aactttgta agacttgaa aacgatgtg aaataaaag 
      tctcatatg cca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY