Status
Please wait ...
Gene information
General information
TC030750 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9390331..9392715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030750
    iBeetle screen iB_06062

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 3

    tropharium structure irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030750 RA
      MADILVLDW MEEFESLTE SEIHTYSSE QEHNHEVMV 
      ALYDILTEP YRFKGQNLI DGICQQLLD FYRSGEEQL 
      KKFTLQYIP TLVFLHLTE KTYTSVQTL LVSLYNLEV 
      IDSKGQPRT LSFRVPSIA QSSIYHDSN NLEPAFIAE 
      NSLRRWEEC NSKLVSWGP LPQVECLNA QNRQRVITA 
      LVFLYNQQL ASVISQGIE HTCRGISRL VSQGFSSND 
      SARSSINSD CSYSQGIAP RIPVSSPLL LELLHIVYH 
      GAEKGVNGA VQALHDITQ RAKYEAFAD VLLSANAIK 
      NLIQRSSSV SVTPRPSQS HSISKSMIT NASFRTKKL 
      PDDIPIQDE HQTEVPLDS ITEEQEEAD KSKPRGSVS 
      ALKHLPKLP GLGKKHKVK NPPSGGDQV EMQNAGGES 
      TAESPSTDF NHAVHVSAV
    CDS sequence
    • >TC030750 RA
      atggcagat atcctcgtc ttggactgg atggaggag 
      ttcgagtcg ctcactgaa agcgaaatc cacacctac 
      tcatcagaa caagaacat aatcacgaa gttatggtc 
      gcactttac gacattcta acggagccc tataggttt 
      aaagggcaa aatctgatt gacggcatt tgccaacag 
      ctgctggac ttttacaga tcaggggag gaacaactc 
      aagaagttc acactgcag tatatacca actttagta 
      tttttacat ttaactgag aaaacgtac acttcggta 
      caaaccctt ttagttagt ttatacaat ttggaggtc 
      atagattct aaagggcag ccccggacc ctttcattc 
      cgggtccca agcatagct cagtcgtcg atataccac 
      gattccaac aacttggaa ccggctttt atcgccgaa 
      aattcgcta cggcggtgg gaagaatgc aacagtaaa 
      ttagtgagt tgggggccc ctgccacaa gttgagtgt 
      ctgaatgct cagaaccgc caacgtgta atcaccgca 
      cttgtgttc ctatataac caacaacta gctagtgtc 
      atatcccag ggtattgag catacttgt agggggatt 
      tctagatta gtttcacaa ggattttct tccaatgat 
      agtgcaaga agttcaata aatagtgat tgtagttat 
      agccaaggt attgctcct cgcattccc gtctctagt 
      ccattactt ttagaacta cttcacata gtgtatcac 
      ggggctgag aagggcgtg aacggggcc gtccaggcc 
      ctccatgat atcacgcaa cgggcgaaa tacgaggca 
      tttgccgac gtgctgttg tccgcaaac gcgataaaa 
      aatctgatc caacgctcg tcctccgtg tctgtaacc 
      ccgcgaccg tcacaaagt cacagcatt tcgaaatca 
      atgatcaca aacgcgtca ttccgaacg aaaaaacta 
      cctgatgat ataccaatc caggatgag catcagaca 
      gaggttcct ttagattct ataacagag gagcaggaa 
      gaggctgac aaaagcaag ccgagaggg tcagttagc 
      gcactcaag catttgcct aaattgccc ggcttaggg 
      aaaaaacac aaagtgaag aatcctcca agtggagga 
      gatcaagtt gaaatgcaa aatgcgggg ggagagtca 
      acagcagag tcgccgtcg acagacttt aaccatgct 
      gtgcacgtt agtgcggtt tag
    mRNA sequence
    • >TC030750 RA
      ctccacatc cataaatca ccctttttg acaagtttg 
      ttttaatct attcaattt gttattttt aacgcattg 
      ttgggccaa atagtgata agatgtact taaataagc 
      agtcgcatt agtgtattc ataagtgtg tttaacggg 
      attcgatga catagattt cgaaaaagt aacctcaaa 
      gttacaacc ccttaattt tcttattat tgtaacaaa 
      aacaggaga caataatgg cagatatcc tcgtcttgg 
      actggatgg aggagttcg agtcgctca ctgaaagcg 
      aaatccaca cctactcat cagaacaag aacataatc 
      acgaagtta tggtcgcac tttacgaca ttctaacgg 
      agccctata ggtttaaag ggcaaaatc tgattgacg 
      gcatttgcc aacagctgc tggactttt acagatcag 
      gggaggaac aactcaaga agttcacac tgcagtata 
      taccaactt tagtatttt tacatttaa ctgagaaaa 
      cgtacactt cggtacaaa cccttttag ttagtttat 
      acaatttgg aggtcatag attctaaag ggcagcccc 
      ggacccttt cattccggg tcccaagca tagctcagt 
      cgtcgatat accacgatt ccaacaact tggaaccgg 
      cttttatcg ccgaaaatt cgctacggc ggtgggaag 
      aatgcaaca gtaaattag tgagttggg ggcccctgc 
      cacaagttg agtgtctga atgctcaga accgccaac 
      gtgtaatca ccgcacttg tgttcctat ataaccaac 
      aactagcta gtgtcatat cccagggta ttgagcata 
      cttgtaggg ggatttcta gattagttt cacaaggat 
      tttcttcca atgatagtg caagaagtt caataaata 
      gtgattgta gttatagcc aaggtattg ctcctcgca 
      ttcccgtct ctagtccat tacttttag aactacttc 
      acatagtgt atcacgggg ctgagaagg gcgtgaacg 
      gggccgtcc aggccctcc atgatatca cgcaacggg 
      cgaaatacg aggcatttg ccgacgtgc tgttgtccg 
      caaacgcga taaaaaatc tgatccaac gctcgtcct 
      ccgtgtctg taaccccgc gaccgtcac aaagtcaca 
      gcatttcga aatcaatga tcacaaacg cgtcattcc 
      gaacgaaaa aactacctg atgatatac caatccagg 
      atgagcatc agacagagg ttcctttag attctataa 
      cagaggagc aggaagagg ctgacaaaa gcaagccga 
      gagggtcag ttagcgcac tcaagcatt tgcctaaat 
      tgcccggct tagggaaaa aacacaaag tgaagaatc 
      ctccaagtg gaggagatc aagttgaaa tgcaaaatg 
      cggggggag agtcaacag cagagtcgc cgtcgacag 
      actttaacc atgctgtgc acgttagtg cggtttagg 
      aacaacaga gtttttcaa atgacttat tttttctaa 
      caaggtata caatgaagt tgatatagg tgttgttta 
      gcgctaggg actactaat caaccaaat atatttttt 
      aaagtatga atgcgacaa ggggcgtct tgagggggt 
      ttcgatgca ccagagcag taggttttg agtagatct 
      tagcgaatt cacttagtt ctgacagca cgtcttact 
      tgaacttaa tttaactga aagaagtat ttttattta 
      agtgcttgg ttgagtttt atttattta ctgattcaa 
      cacgacgaa atgggggtt tcattttcg tgatacatt 
      ggttatatt tcaggtttt ttacacgag acgaaaaat 
      tttttttac agagctgga tctagtcat gataaaaac 
      gtaattggc accctttta agactaaac tgaacaata 
      ttttacttc gatccggct ctgtaaagt tatttgata 
      ctattattt atcatttct ttattggtc ataattaga 
      ggtcctaac agagaacaa atttggagt tatgactgt 
      ggtgcggat tttttaaaa tttagttaa aaatacata 
      aacaaattg aaaatacga tacacgttg ttactacaa 
      aaggtcagc ttaaagtag ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY