General information | |||
TC030748 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 5821197..5828255 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC030748 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06060 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen posterior partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC030748 RA
MELRSRSRS KTPFQTQTF INDEAFEGH NHVTRSTRR SVRTTVTTG GSEYYESVT KPVKSKSTT KSTRSIFKT SDYSSEEGE TDSHSHFSE NEKNQIIED ARKAANGTA EISALSLYR KAGRYWDKY PKTDYTYSP HSRDRVELA PGVVQMPNM SRRTIHSDS STNTESYYT TSQSIKQRL LSSSNNNEE IDESYTISR TSTTRRRTF TSIITSVFT TIYMSVFWC FLSVYETSS STMIFFGKK LHHLASLIM LADTWLLRK SSAPRKTGA VVALCLLPL LVFAAWFLL SSLGSAIYW SFTNSTSIP VEQQVAEKI ISAPPVPSP TQINTDEII EKILQNPKI HNIIINNHK GQESDEKFQ HIIEELRLE IDRIKSEQQ NQNADLGRI IAQIRTENV RNLARLTQK LNRCCSRQI IDLEPYITR VFTNLLNDP HFLSNQNGL TDWLHTLFV AKTDLENRL LNLTTNFDV SDAANQVME KVVGKLSKL DPSSLDETQ IRKIVQSAL RIYDADKTG LVDYAMERL GGEIVTTRC TESYFAGTA VISVLGIPI WYPSISPRT VITPGINPG ECWAFQNFP GLLVIKLAS RVRIEAFSL EHVSRLLVP EGKIDSAPK EFEVFGLNG ENDKDPVWL GEFVYDYDG DPLQFFAVR EPKVCSMIE IRIKSNHGN PNYTCLYRF RVHGKVSNE PA
- >TC030748 RA
atggagtta aggtcgaga tccaggtct aagacgccg tttcagacg caaactttt ataaatgac gaggccttt gaagggcac aaccatgtg acgagatcg acgagacgt tcggtcaga acaacggtg actactggt ggcagcgag tattatgaa agtgtgacc aaaccagtg aagagcaaa agtacaaca aaatcgacg cgttcgata tttaagact tctgattat tcgtcagag gaaggtgaa acagattcg cattcccac ttttctgaa aatgagaaa aatcagatc attgaagat gcaagaaag gcagctaat ggcactgct gagatctca gccctgagc ttgtacagg aaagcgggg agatattgg gacaaatat cctaagacc gattatacg tattcgcca cattcgcgg gatcgagtc gagctggcc cctggtgtg gtccaaatg cccaacatg tcccgaagg accatccac tcagactcc agcaccaac actgaatcc tactataca acgtctcaa tccatcaag caaagactg ctctcatcg tcaaacaac aatgaagaa atcgatgaa agttacaca attagtcga acctcgacc acacgaaga cgcacattt acatcgatc atcacttcg gtcttcacg accatctac atgtcggtg ttttggtgc ttcctgtca gtgtatgag acgtccagt tcgacgatg attttcttc ggtaagaag ttgcaccac ttggcgagt ctgattatg ctggctgat acgtggctg ctgaggaaa tcctcagcg ccgaggaag acgggggct gtggtggcg ttgtgtctt ctccccctt ttggttttt gcagcttgg tttttactg tcgtcactg ggcagtgcc atctattgg tcttttacc aacagtact tccatccct gtagaacaa caagtagca gaaaaaata ataagtgca ccgcctgtc ccatcaccc actcaaata aacacagac gaaattatc gaaaaaatc ctccaaaac ccaaaaatc cacaacatt attattaat aaccacaag gggcaagaa tctgacgag aaattccaa cacataatt gaggaactc cggctcgaa attgatcga attaaaagt gagcaacag aatcaaaat gccgatttg ggccgaatt attgcacaa atccgaacc gaaaacgtc cgaaatttg gcccgtttg acccaaaag ctgaatagg tgttgcagt cgccaaatc atcgatttg gaaccttac ataacgcgc gtttttaca aacttgctc aacgaccct cacttttta tcaaaccag aacggttta accgactgg ttacataca ctgttcgtt gcgaaaacc gatctcgaa aaccgtcta ctgaaccta acaaccaac tttgacgtt tcggatgca gccaatcaa gttatggaa aaagtggtt gggaagtta tccaagttg gaccccagc tcgcttgat gaaacgcaa atcaggaaa atcgtccaa tcagcgttg cgcatttac gacgctgat aaaaccgga ttggtcgat tacgcgatg gagcgtttg ggtggggag atcgtgact actagatgt accgagagt tattttgcg ggtactgcg gttatttcg gttctgggg attcccatc tggtatccg tcgatttcc ccgcggact gttatcact ccggggata aatccgggc gagtgttgg gcgttccag aattttccg ggtttgttg gtcattaag ttggcgtcg agggtgaga atcgaggcg ttttcgttg gagcatgtt agtcggttg ctggtgcct gagggtaaa attgattcg gcgccgaag gagtttgag gtttttggg ttgaatggg gagaatgat aaagatccg gtttggtta ggggagttt gtttatgac tatgacggt gatccgttg cagtttttc gcggttagg gaaccaaag gtgtgttct atgatcgag atcaggatt aaatcgaat catggcaat ccgaattat acttgtttg taccggttt agggttcac gggaaggtt agtaatgaa ccggcgtag
- >TC030748 RA
gcatctgta cttgtacta atttttagt attatgttt acgttaaaa ccgttggaa tttggtttt ttttgatca ttaaactcg tttgaattt cggtgacgg ttgctttat tgtgtccat gattggtgt gttgaacgt tcggtgaag tctggaaat catggagtt aaggtcgag atccaggtc taagacgcc gtttcagac gcaaacttt tataaatga cgaggcctt tgaagggca caaccatgt gacgagatc gacgagacg ttcggtcag aacaacggt gactactgg tggcagcga gtattatga aagtgtgac caaaccagt gaagagcaa aagtacaac aaaatcgac gcgttcgat atttaagac ttctgatta ttcgtcaga ggaaggtga aacagattc gcattccca cttttctga aaatgagaa aaatcagat cattgaaga tgcaagaaa ggcagctaa tggcactgc tgagatctc agccctgag cttgtacag gaaagcggg gagatattg ggacaaata tcctaagac cgattatac gtattcgcc acattcgcg ggatcgagt cgagctggc ccctggtgt ggtccaaat gcccaacat gtcccgaag gaccatcca ctcagactc cagcaccaa cactgaatc ctactatac aacgtctca atccatcaa gcaaagact gctctcatc gtcaaacaa caatgaaga aatcgatga aagttacac aattagtcg aacctcgac cacacgaag acgcacatt tacatcgat catcacttc ggtcttcac gaccatcta catgtcggt gttttggtg cttcctgtc agtgtatga gacgtccag ttcgacgat gattttctt cggtaagaa gttgcacca cttggcgag tctgattat gctggctga tacgtggct gctgaggaa atcctcagc gccgaggaa gacgggggc tgtggtggc gttgtgtct tctccccct tttggtttt tgcagcttg gtttttact gtcgtcact gggcagtgc catctattg gtcttttac caacagtac ttccatccc tgtagaaca acaagtagc agaaaaaat aataagtgc accgcctgt cccatcacc cactcaaat aaacacaga cgaaattat cgaaaaaat cctccaaaa cccaaaaat ccacaacat tattattaa taaccacaa ggggcaaga atctgacga gaaattcca acacataat tgaggaact ccggctcga aattgatcg aattaaaag tgagcaaca gaatcaaaa tgccgattt gggccgaat tattgcaca aatccgaac cgaaaacgt ccgaaattt ggcccgttt gacccaaaa gctgaatag gtgttgcag tcgccaaat catcgattt ggaacctta cataacgcg cgtttttac aaacttgct caacgaccc tcacttttt atcaaacca gaacggttt aaccgactg gttacatac actgttcgt tgcgaaaac cgatctcga aaaccgtct actgaacct aacaaccaa ctttgacgt ttcggatgc agccaatca agttatgga aaaagtggt tgggaagtt atccaagtt ggaccccag ctcgcttga tgaaacgca aatcaggaa aatcgtcca atcagcgtt gcgcattta cgacgctga taaaaccgg attggtcga ttacgcgat ggagcgttt gggtgggga gatcgtgac tactagatg taccgagag ttattttgc gggtactgc ggttatttc ggttctggg gattcccat ctggtatcc gtcgatttc cccgcggac tgttatcac tccggggat aaatccggg cgagtgttg ggcgttcca gaattttcc gggtttgtt ggtcattaa gttggcgtc gagggtgag aatcgaggc gttttcgtt ggagcatgt tagtcggtt gctggtgcc tgagggtaa aattgattc ggcgccgaa ggagtttga ggtttttgg gttgaatgg ggagaatga taaagatcc ggtttggtt aggggagtt tgtttatga ctatgacgg tgatccgtt gcagttttt cgcggttag ggaaccaaa ggtgtgttc tatgatcga gatcaggat taaatcgaa tcatggcaa tccgaatta tacttgttt gtaccggtt tagggttca cgggaaggt tagtaatga accggcgta ggttttaac cgtttttgt tttgttagg tcatgtgtt cattattaa tttatttgt actgttgta aatacctcg tctattttc gtttcttgc cgtcttttt agtacttat gtgtgtttt atatctcat tctttttag cgtttttca ttggtattt tacttgttt tttatgtat taataatga aagtctgtg attcggtct aaactatag ttaagtaca aaaataatg ttgtaaagt aggtactac cggaaataa taaggcctt atgcaattt tgcaaacct aatcttagt cacgtgttt cagtcacta gttttaggg tctaatatt tatcttaag ggtcattgt caccgatgc acgctcatt tgtattttc gtatgtgaa taaagtttt tgttacgat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
