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Gene information
General information
TC030748 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5821197..5828255
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030748
    iBeetle screen iB_06060

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen posterior partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030748 RA
      MELRSRSRS KTPFQTQTF INDEAFEGH NHVTRSTRR 
      SVRTTVTTG GSEYYESVT KPVKSKSTT KSTRSIFKT 
      SDYSSEEGE TDSHSHFSE NEKNQIIED ARKAANGTA 
      EISALSLYR KAGRYWDKY PKTDYTYSP HSRDRVELA 
      PGVVQMPNM SRRTIHSDS STNTESYYT TSQSIKQRL 
      LSSSNNNEE IDESYTISR TSTTRRRTF TSIITSVFT 
      TIYMSVFWC FLSVYETSS STMIFFGKK LHHLASLIM 
      LADTWLLRK SSAPRKTGA VVALCLLPL LVFAAWFLL 
      SSLGSAIYW SFTNSTSIP VEQQVAEKI ISAPPVPSP 
      TQINTDEII EKILQNPKI HNIIINNHK GQESDEKFQ 
      HIIEELRLE IDRIKSEQQ NQNADLGRI IAQIRTENV 
      RNLARLTQK LNRCCSRQI IDLEPYITR VFTNLLNDP 
      HFLSNQNGL TDWLHTLFV AKTDLENRL LNLTTNFDV 
      SDAANQVME KVVGKLSKL DPSSLDETQ IRKIVQSAL 
      RIYDADKTG LVDYAMERL GGEIVTTRC TESYFAGTA 
      VISVLGIPI WYPSISPRT VITPGINPG ECWAFQNFP 
      GLLVIKLAS RVRIEAFSL EHVSRLLVP EGKIDSAPK 
      EFEVFGLNG ENDKDPVWL GEFVYDYDG DPLQFFAVR 
      EPKVCSMIE IRIKSNHGN PNYTCLYRF RVHGKVSNE 
      PA
    CDS sequence
    • >TC030748 RA
      atggagtta aggtcgaga tccaggtct aagacgccg 
      tttcagacg caaactttt ataaatgac gaggccttt 
      gaagggcac aaccatgtg acgagatcg acgagacgt 
      tcggtcaga acaacggtg actactggt ggcagcgag 
      tattatgaa agtgtgacc aaaccagtg aagagcaaa 
      agtacaaca aaatcgacg cgttcgata tttaagact 
      tctgattat tcgtcagag gaaggtgaa acagattcg 
      cattcccac ttttctgaa aatgagaaa aatcagatc 
      attgaagat gcaagaaag gcagctaat ggcactgct 
      gagatctca gccctgagc ttgtacagg aaagcgggg 
      agatattgg gacaaatat cctaagacc gattatacg 
      tattcgcca cattcgcgg gatcgagtc gagctggcc 
      cctggtgtg gtccaaatg cccaacatg tcccgaagg 
      accatccac tcagactcc agcaccaac actgaatcc 
      tactataca acgtctcaa tccatcaag caaagactg 
      ctctcatcg tcaaacaac aatgaagaa atcgatgaa 
      agttacaca attagtcga acctcgacc acacgaaga 
      cgcacattt acatcgatc atcacttcg gtcttcacg 
      accatctac atgtcggtg ttttggtgc ttcctgtca 
      gtgtatgag acgtccagt tcgacgatg attttcttc 
      ggtaagaag ttgcaccac ttggcgagt ctgattatg 
      ctggctgat acgtggctg ctgaggaaa tcctcagcg 
      ccgaggaag acgggggct gtggtggcg ttgtgtctt 
      ctccccctt ttggttttt gcagcttgg tttttactg 
      tcgtcactg ggcagtgcc atctattgg tcttttacc 
      aacagtact tccatccct gtagaacaa caagtagca 
      gaaaaaata ataagtgca ccgcctgtc ccatcaccc 
      actcaaata aacacagac gaaattatc gaaaaaatc 
      ctccaaaac ccaaaaatc cacaacatt attattaat 
      aaccacaag gggcaagaa tctgacgag aaattccaa 
      cacataatt gaggaactc cggctcgaa attgatcga 
      attaaaagt gagcaacag aatcaaaat gccgatttg 
      ggccgaatt attgcacaa atccgaacc gaaaacgtc 
      cgaaatttg gcccgtttg acccaaaag ctgaatagg 
      tgttgcagt cgccaaatc atcgatttg gaaccttac 
      ataacgcgc gtttttaca aacttgctc aacgaccct 
      cacttttta tcaaaccag aacggttta accgactgg 
      ttacataca ctgttcgtt gcgaaaacc gatctcgaa 
      aaccgtcta ctgaaccta acaaccaac tttgacgtt 
      tcggatgca gccaatcaa gttatggaa aaagtggtt 
      gggaagtta tccaagttg gaccccagc tcgcttgat 
      gaaacgcaa atcaggaaa atcgtccaa tcagcgttg 
      cgcatttac gacgctgat aaaaccgga ttggtcgat 
      tacgcgatg gagcgtttg ggtggggag atcgtgact 
      actagatgt accgagagt tattttgcg ggtactgcg 
      gttatttcg gttctgggg attcccatc tggtatccg 
      tcgatttcc ccgcggact gttatcact ccggggata 
      aatccgggc gagtgttgg gcgttccag aattttccg 
      ggtttgttg gtcattaag ttggcgtcg agggtgaga 
      atcgaggcg ttttcgttg gagcatgtt agtcggttg 
      ctggtgcct gagggtaaa attgattcg gcgccgaag 
      gagtttgag gtttttggg ttgaatggg gagaatgat 
      aaagatccg gtttggtta ggggagttt gtttatgac 
      tatgacggt gatccgttg cagtttttc gcggttagg 
      gaaccaaag gtgtgttct atgatcgag atcaggatt 
      aaatcgaat catggcaat ccgaattat acttgtttg 
      taccggttt agggttcac gggaaggtt agtaatgaa 
      ccggcgtag
    mRNA sequence
    • >TC030748 RA
      gcatctgta cttgtacta atttttagt attatgttt 
      acgttaaaa ccgttggaa tttggtttt ttttgatca 
      ttaaactcg tttgaattt cggtgacgg ttgctttat 
      tgtgtccat gattggtgt gttgaacgt tcggtgaag 
      tctggaaat catggagtt aaggtcgag atccaggtc 
      taagacgcc gtttcagac gcaaacttt tataaatga 
      cgaggcctt tgaagggca caaccatgt gacgagatc 
      gacgagacg ttcggtcag aacaacggt gactactgg 
      tggcagcga gtattatga aagtgtgac caaaccagt 
      gaagagcaa aagtacaac aaaatcgac gcgttcgat 
      atttaagac ttctgatta ttcgtcaga ggaaggtga 
      aacagattc gcattccca cttttctga aaatgagaa 
      aaatcagat cattgaaga tgcaagaaa ggcagctaa 
      tggcactgc tgagatctc agccctgag cttgtacag 
      gaaagcggg gagatattg ggacaaata tcctaagac 
      cgattatac gtattcgcc acattcgcg ggatcgagt 
      cgagctggc ccctggtgt ggtccaaat gcccaacat 
      gtcccgaag gaccatcca ctcagactc cagcaccaa 
      cactgaatc ctactatac aacgtctca atccatcaa 
      gcaaagact gctctcatc gtcaaacaa caatgaaga 
      aatcgatga aagttacac aattagtcg aacctcgac 
      cacacgaag acgcacatt tacatcgat catcacttc 
      ggtcttcac gaccatcta catgtcggt gttttggtg 
      cttcctgtc agtgtatga gacgtccag ttcgacgat 
      gattttctt cggtaagaa gttgcacca cttggcgag 
      tctgattat gctggctga tacgtggct gctgaggaa 
      atcctcagc gccgaggaa gacgggggc tgtggtggc 
      gttgtgtct tctccccct tttggtttt tgcagcttg 
      gtttttact gtcgtcact gggcagtgc catctattg 
      gtcttttac caacagtac ttccatccc tgtagaaca 
      acaagtagc agaaaaaat aataagtgc accgcctgt 
      cccatcacc cactcaaat aaacacaga cgaaattat 
      cgaaaaaat cctccaaaa cccaaaaat ccacaacat 
      tattattaa taaccacaa ggggcaaga atctgacga 
      gaaattcca acacataat tgaggaact ccggctcga 
      aattgatcg aattaaaag tgagcaaca gaatcaaaa 
      tgccgattt gggccgaat tattgcaca aatccgaac 
      cgaaaacgt ccgaaattt ggcccgttt gacccaaaa 
      gctgaatag gtgttgcag tcgccaaat catcgattt 
      ggaacctta cataacgcg cgtttttac aaacttgct 
      caacgaccc tcacttttt atcaaacca gaacggttt 
      aaccgactg gttacatac actgttcgt tgcgaaaac 
      cgatctcga aaaccgtct actgaacct aacaaccaa 
      ctttgacgt ttcggatgc agccaatca agttatgga 
      aaaagtggt tgggaagtt atccaagtt ggaccccag 
      ctcgcttga tgaaacgca aatcaggaa aatcgtcca 
      atcagcgtt gcgcattta cgacgctga taaaaccgg 
      attggtcga ttacgcgat ggagcgttt gggtgggga 
      gatcgtgac tactagatg taccgagag ttattttgc 
      gggtactgc ggttatttc ggttctggg gattcccat 
      ctggtatcc gtcgatttc cccgcggac tgttatcac 
      tccggggat aaatccggg cgagtgttg ggcgttcca 
      gaattttcc gggtttgtt ggtcattaa gttggcgtc 
      gagggtgag aatcgaggc gttttcgtt ggagcatgt 
      tagtcggtt gctggtgcc tgagggtaa aattgattc 
      ggcgccgaa ggagtttga ggtttttgg gttgaatgg 
      ggagaatga taaagatcc ggtttggtt aggggagtt 
      tgtttatga ctatgacgg tgatccgtt gcagttttt 
      cgcggttag ggaaccaaa ggtgtgttc tatgatcga 
      gatcaggat taaatcgaa tcatggcaa tccgaatta 
      tacttgttt gtaccggtt tagggttca cgggaaggt 
      tagtaatga accggcgta ggttttaac cgtttttgt 
      tttgttagg tcatgtgtt cattattaa tttatttgt 
      actgttgta aatacctcg tctattttc gtttcttgc 
      cgtcttttt agtacttat gtgtgtttt atatctcat 
      tctttttag cgtttttca ttggtattt tacttgttt 
      tttatgtat taataatga aagtctgtg attcggtct 
      aaactatag ttaagtaca aaaataatg ttgtaaagt 
      aggtactac cggaaataa taaggcctt atgcaattt 
      tgcaaacct aatcttagt cacgtgttt cagtcacta 
      gttttaggg tctaatatt tatcttaag ggtcattgt 
      caccgatgc acgctcatt tgtattttc gtatgtgaa 
      taaagtttt tgttacgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY