Status
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Gene information
General information
TC032139
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030770,TC030746
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9910470..9917330
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032139
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06059

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032139 RA
      MAQTVSDYY DGSRLFSGL SEYLGISVD KVNFVISQV 
      GALFLASLF RNVLHPSKA NAATRHAFG LVFALIIGY 
      FCFGTQAIH LAALPAACY IVIRTQSPE VMQRMVLAV 
      ALVYLSVIH LHRQIYDYG SYNLDITGP LMVITQKVT 
      SLAFNIHDG LTKHQEKLS KSQKSYAVT KIPNPLEYF 
      SFVLHFPSL MAGPAMLYK EYMDFIDGS NLKSPVLAD 
      TPTRNAPIV VEPSPTWAV CKKVAIAAG CAAIFVKFL 
      PMFPISRVK ENDFVENTS ITQKIVYLY ICTTLVRFK 
      YYFAWTLAD AVCNNAGIG YNGVDASGA HRWDKFSNV 
      DIFQFEVSV FYGSATSSS ASDGGTTDI SESFSDNGT 
      NQRNITLKF LYGDNKVFT QSYHEDTAI GDLKTLLTE 
      VFNTPTNNL KLLLGEKEL NDDSILNEL GAEPFGVLE 
      LRLLSTNSD DKLSIDEAF RDYTIPDVI TVRVQTSDE 
      GIKDVVVEV ENRAIIKPF LGGYVDTHS GLEYHHGYT 
      QTGPPKPKI PPEMKNHRD TQTYFMRNR KLDMPYGHA 
      TQMAAENLW IPAVGDRIL TPGPYETAD EREKRLDVE 
      GKVRVIQRY FRAWLTRKR LKEYSEEYH KRIRLMLER 
      EELERREDL ERKKRDLIS RVFPRTAAD FSMLYAMTE 
      RWKKAEIKR IIEMHCGPA RIAEFYLLL EKEIQILRC 
      IDELRLKLK NDRKIQKEI DFFKTIGDP WTFKSTYKN 
      IPVVVDTLE TQKGRTFRE LYEAICDPT LDGSNRMLV 
      FLELRRGLE SHNCVISNE LVDLIDRAC ELLARGFES 
      RHIETLQKR IEALLLHHF QQSECNEGV TNRYNRLTE 
      KAMKENLFY CHSCKKLKP IECYNVGVK TNAAKICAT 
      CRFIDRSKE PWIDISPYR FILRQIRKN ERLLHALSS 
      IAFIIQDKD IYYLVTHIW HGHSALSEC DNIYQLRLC 
      RWDKTKDWS PWNCILLTA EEAKAHLKI DKLEDVYDE 
      HFLSNVFNK HALTKKYFA QSFDIDKCV RETYVDDDY 
      FATNLRESI AAWNLGTNR WLRMIVYER VSKYSMVLT 
      YSLSALWHG FYPGYYLTF ANGALFTYA SRVLRKRVR 
      NYFLANAET KFFYDGVTF VTTRILMAY MVFTFVLLE 
      FWPCIRLYM HMYWCLHLG ALLAIFLVP NVIPKTEAG 
      QAVKGSIAQ AFVQARPIT NGVSRHHD
    CDS sequence
    • >TC032139 RA
      atggctcaa actgttagc gattattac gacggaagc 
      cggcttttt tcaggactt tcagaatat ttgggcatt 
      tcagtcgac aaggtaaat tttgttatc agccaagtt 
      ggggcactt ttcttggcc tcgctcttt cgcaatgtg 
      ctccaccca tcgaaagca aatgcagcc acaagacac 
      gcttttggg ctcgtgttt gccctcatc atcggctat 
      ttctgtttc gggacacag gctatacac ttagcggcc 
      cttccggca gcatgctat attgttatc agaacgcaa 
      agtccagaa gtcatgcaa agaatggtt ctagcagtt 
      gcattagtt tatttatct gttatacac ttgcatcga 
      cagatatac gactatggg tcctataat ctcgacatc 
      actggacct ctaatggtg atcactcag aaggtcaca 
      agtcttgca tttaacatc cacgacggc ttgaccaaa 
      catcaggag aagctctca aaaagccaa aaatcctac 
      gccgtcaca aaaatcccc aatcctttg gaatatttt 
      agtttcgta ttgcatttc ccgtcttta atggccggc 
      cctgccatg ttatacaaa gagtacatg gactttatc 
      gatggcagt aatttaaaa tcgccggtt ttggcagac 
      actccaacc aggaacgcc ccaattgtt gtagaaccg 
      tcacctacg tgggctgtg tgcaaaaaa gtggccata 
      gcagctgga tgtgctgca attttcgtt aaattttta 
      ccaatgttt ccaataagt cgcgtcaaa gagaacgac 
      tttgtggaa aacacgagc atcacccag aaaattgtg 
      tacctgtat atttgtaca actctagtt aggtttaag 
      tattatttc gcctggaca ttggcggac gccgtgtgc 
      aataacgcg ggcataggg tataacggg gtggacgcc 
      agtggcgcc cacaggtgg gacaagttc tcgaatgtc 
      gacatattt caatttgag gtaagtgtc ttttacggt 
      tcggcaact tcaagtagt gcttccgat ggtggtaca 
      actgacatt tctgaatct ttttcggac aatggcact 
      aatcaaaga aacatcact cttaaattc ctctacggc 
      gataacaaa gttttcacg caatcctac cacgaggac 
      acggcaatc ggcgacttg aaaacgctc ctaaccgaa 
      gttttcaat actccaacc aacaacctt aaactccta 
      cttggcgaa aaagagcta aacgatgac tcaattttg 
      aacgaactg ggggccgaa cctttcgga gtcctcgaa 
      ctgagacta ctttcgaca aattctgac gataaatta 
      agcattgac gaagcgttc cgtgactac acaatcccc 
      gatgtgata acagtccgc gtccagaca tcggacgaa 
      ggcatcaaa gacgtggtt gtcgaagtg gaaaatcgt 
      gctataatc aaaccgttt ttgggcggc tatgtcgac 
      acgcactcg ggcctggag taccatcat gggtacacc 
      cagacgggc ccacccaag cccaaaatc ccgcccgaa 
      atgaaaaac cacagagac acccagacc tatttcatg 
      cggaaccgg aaactggac atgccgtac ggacacgcc 
      acgcaaatg gccgccgaa aacttgtgg attccggcg 
      gtgggcgac cggattttg actccgggc ccgtacgaa 
      acggccgac gagagggag aagcggctg gacgttgag 
      gggaaagtc cgggtgatc cagcgctat ttccgggcg 
      tggttgact aggaaaaga ttgaaggag tacagtgag 
      gagtatcat aaaaggatt aggttgatg ttggaacgc 
      gaagagttg gagcgcagg gaggatttg gagcggaag 
      aaacgtgat ttgatcagt cgggtgttt ccccggacc 
      gcggccgat ttctccatg ctgtacgcg atgacggaa 
      cggtggaaa aaagccgag attaagagg atcatagaa 
      atgcattgc gggccggca cggatcgcc gaattctac 
      ctcctgttg gagaaggaa attcaaatt ttgcgctgc 
      atcgacgag ttgagactc aagttgaag aacgatcgc 
      aaaattcag aaggaaatt gattttttt aaaacaata 
      ggcgacccc tggactttc aaatccact tacaaaaac 
      attccagtg gttgtggac acgcttgag acgcaaaaa 
      gggcgcaca tttcgcgag ttgtacgaa gcgatttgt 
      gacccaact ctggacggg tcaaaccgg atgctggtt 
      tttctggaa ctgagacgg ggcttggag agccacaat 
      tgcgtaatc tccaatgaa ttggtcgat ttaatcgat 
      cgtgcgtgc gaattgttg gcacgagga ttcgagagt 
      cgacatatc gagactttg cagaaaaga atcgaagct 
      ttgttactg caccatttc cagcaaagc gagtgtaac 
      gaaggtgtc actaatcgc tacaaccgc ctgaccgag 
      aaggcaatg aaagagaat ctgttctat tgtcacagt 
      tgtaaaaaa ctcaagcct attgagtgt tacaacgtt 
      ggggtgaag acaaacgcg gcgaaaatc tgtgccact 
      tgtcgattc atcgatcgc tcgaaagag ccatggatt 
      gatatttcc ccctatcga tttattttg cgacaaatc 
      cgcaaaaac gaacgtctt ttgcacgct ttatcctcg 
      atcgcgttt attatccaa gacaaggat atttactat 
      cttgtgacg catatctgg cacggccat tcggcccta 
      tcagagtgt gataacatc taccagctg cggctgtgt 
      cgctgggac aaaaccaaa gactggtcg ccctggaac 
      tgcatcctc ctaacagcg gaagaagcc aaagcgcat 
      ttgaaaatc gacaaactc gaagacgtc tacgacgag 
      catttccta agcaatgtt ttcaacaaa cacgctctg 
      actaaaaaa tacttcgcc cagagtttc gatatagac 
      aagtgcgtg cgtgaaaca tacgtcgac gatgactat 
      ttcgctacg aatttgcgc gaaagcata gcagcttgg 
      aacttgggc acgaaccgg tggctgcgt atgattgtg 
      tatgagcgt gtgagtaaa tattcgatg gttttgacc 
      tactccttg tccgccctc tggcacggg ttctacccc 
      ggctattac ctcacgttc gcaaacggg gccctgttc 
      acgtacgcc tcgcgtgtg ttgcgtaaa cgagtgagg 
      aattatttt ctggcaaac gccgaaacg aaattcttt 
      tacgacgga gtgacgttc gtgacgaca cgaatacta 
      atggcttac atggtgttc acgtttgtg ttacttgag 
      ttttggccc tgcattcga ttatacatg cacatgtat 
      tggtgtctg catctgggg gccctcctg gcgattttt 
      cttgtgcct aatgtaatt ccaaagact gaagcggga 
      caagcggtg aagggctca atagcacaa gcctttgtt 
      caagcaaga cccatcaca aacggcgta tcgcgacac 
      catgactga
    mRNA sequence
    • >TC032139 RA
      tccttcggc aaacgtcgg ctcgcgccg acgttgcca 
      attgacaaa cgagcatcg tggcgtgac tgcataaaa 
      caatcgcac cgcattggt gtgcacagc ctacttgta 
      ataattggt aaacaaact gtatcattc aattttttt 
      gaacaaatg atagttgag tgaaattgt tgagcgtgt 
      ggagaccct gaaaatggc tcaaactgt tagcgatta 
      ttacgacgg aagccggct tttttcagg actttcaga 
      atatttggg catttcagt cgacaaggt aaattttgt 
      tatcagcca agttggggc acttttctt ggcctcgct 
      ctttcgcaa tgtgctcca cccatcgaa agcaaatgc 
      agccacaag acacgcttt tgggctcgt gtttgccct 
      catcatcgg ctatttctg tttcgggac acaggctat 
      acacttagc ggcccttcc ggcagcatg ctatattgt 
      tatcagaac gcaaagtcc agaagtcat gcaaagaat 
      ggttctagc agttgcatt agtttattt atctgttat 
      acacttgca tcgacagat atacgacta tgggtccta 
      taatctcga catcactgg acctctaat ggtgatcac 
      tcagaaggt cacaagtct tgcatttaa catccacga 
      cggcttgac caaacatca ggagaagct ctcaaaaag 
      ccaaaaatc ctacgccgt cacaaaaat ccccaatcc 
      tttggaata ttttagttt cgtattgca tttcccgtc 
      tttaatggc cggccctgc catgttata caaagagta 
      catggactt tatcgatgg cagtaattt aaaatcgcc 
      ggttttggc agacactcc aaccaggaa cgccccaat 
      tgttgtaga accgtcacc tacgtgggc tgtgtgcaa 
      aaaagtggc catagcagc tggatgtgc tgcaatttt 
      cgttaaatt tttaccaat gtttccaat aagtcgcgt 
      caaagagaa cgactttgt ggaaaacac gagcatcac 
      ccagaaaat tgtgtacct gtatatttg tacaactct 
      agttaggtt taagtatta tttcgcctg gacattggc 
      ggacgccgt gtgcaataa cgcgggcat agggtataa 
      cggggtgga cgccagtgg cgcccacag gtgggacaa 
      gttctcgaa tgtcgacat atttcaatt tgaggtaag 
      tgtctttta cggttcggc aacttcaag tagtgcttc 
      cgatggtgg tacaactga catttctga atctttttc 
      ggacaatgg cactaatca aagaaacat cactcttaa 
      attcctcta cggcgataa caaagtttt cacgcaatc 
      ctaccacga ggacacggc aatcggcga cttgaaaac 
      gctcctaac cgaagtttt caatactcc aaccaacaa 
      ccttaaact cctacttgg cgaaaaaga gctaaacga 
      tgactcaat tttgaacga actgggggc cgaaccttt 
      cggagtcct cgaactgag actactttc gacaaattc 
      tgacgataa attaagcat tgacgaagc gttccgtga 
      ctacacaat ccccgatgt gataacagt ccgcgtcca 
      gacatcgga cgaaggcat caaagacgt ggttgtcga 
      agtggaaaa tcgtgctat aatcaaacc gtttttggg 
      cggctatgt cgacacgca ctcgggcct ggagtacca 
      tcatgggta cacccagac gggcccacc caagcccaa 
      aatcccgcc cgaaatgaa aaaccacag agacaccca 
      gacctattt catgcggaa ccggaaact ggacatgcc 
      gtacggaca cgccacgca aatggccgc cgaaaactt 
      gtggattcc ggcggtggg cgaccggat tttgactcc 
      gggcccgta cgaaacggc cgacgagag ggagaagcg 
      gctggacgt tgaggggaa agtccgggt gatccagcg 
      ctatttccg ggcgtggtt gactaggaa aagattgaa 
      ggagtacag tgaggagta tcataaaag gattaggtt 
      gatgttgga acgcgaaga gttggagcg cagggagga 
      tttggagcg gaagaaacg tgatttgat cagtcgggt 
      gtttccccg gaccgcggc cgatttctc catgctgta 
      cgcgatgac ggaacggtg gaaaaaagc cgagattaa 
      gaggatcat agaaatgca ttgcgggcc ggcacggat 
      cgccgaatt ctacctcct gttggagaa ggaaattca 
      aattttgcg ctgcatcga cgagttgag actcaagtt 
      gaagaacga tcgcaaaat tcagaagga aattgattt 
      ttttaaaac aataggcga cccctggac tttcaaatc 
      cacttacaa aaacattcc agtggttgt ggacacgct 
      tgagacgca aaaagggcg cacatttcg cgagttgta 
      cgaagcgat ttgtgaccc aactctgga cgggtcaaa 
      ccggatgct ggtttttct ggaactgag acggggctt 
      ggagagcca caattgcgt aatctccaa tgaattggt 
      cgatttaat cgatcgtgc gtgcgaatt gttggcacg 
      aggattcga gagtcgaca tatcgagac tttgcagaa 
      aagaatcga agctttgtt actgcacca tttccagca 
      aagcgagtg taacgaagg tgtcactaa tcgctacaa 
      ccgcctgac cgagaaggc aatgaaaga gaatctgtt 
      ctattgtca cagttgtaa aaaactcaa gcctattga 
      gtgttacaa cgttggggt gaagacaaa cgcggcgaa 
      aatctgtgc cacttgtcg attcatcga tcgctcgaa 
      agagccatg gattgatat ttcccccta tcgatttat 
      tttgcgaca aatccgcaa aaacgaacg tcttttgca 
      cgctttatc ctcgatcgc gtttattat ccaagacaa 
      ggatattta ctatcttgt gacgcatat ctggcacgg 
      ccattcggc cctatcaga gtgtgataa catctacca 
      gctgcggct gtgtcgctg ggacaaaac caaagactg 
      gtcgccctg gaactgcat cctcctaac agcggaaga 
      agccaaagc gcatttgaa aatcgacaa actcgaaga 
      cgtctacga cgagcattt cctaagcaa tgttttcaa 
      caaacacgc tctgactaa aaaatactt cgcccagag 
      tttcgatat agacaagtg cgtgcgtga aacatacgt 
      cgacgatga ctatttcgc tacgaattt gcgcgaaag 
      catagcagc ttggaactt gggcacgaa ccggtggct 
      gcgtatgat tgtgtatga gcgtgtgag taaatattc 
      gatggtttt gacctactc cttgtccgc cctctggca 
      cgggttcta ccccggcta ttacctcac gttcgcaaa 
      cggggccct gttcacgta cgcctcgcg tgtgttgcg 
      taaacgagt gaggaatta ttttctggc aaacgccga 
      aacgaaatt cttttacga cggagtgac gttcgtgac 
      gacacgaat actaatggc ttacatggt gttcacgtt 
      tgtgttact tgagttttg gccctgcat tcgattata 
      catgcacat gtattggtg tctgcatct gggggccct 
      cctggcgat ttttcttgt gcctaatgt aattccaaa 
      gactgaagc gggacaagc ggtgaaggg ctcaatagc 
      acaagcctt tgttcaagc aagacccat cacaaacgg 
      cgtatcgcg acaccatga ctgatcaca acgcagctt 
      taattgtcg tattttatc tactggcag taaagagta 
      gttgcacac taataaacc tgttattta ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY