Status
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Gene information
General information
TC030738 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10001932..10015140
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030738
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030738 RA
      MAHYYQSSQ DRYRKEDSE IDISGNEDS DSSAFTEEP 
      QHSKTKKPI NKGRWSKEE DARLKQLVE EYNEKWDVI 
      AELFPDRSD VQCQQRWTK VVNPELVKG PWTKEEDEK 
      VIELVNKYG AKKWTLIAR HLKGRIGKQ CRERWHNHL 
      NPKIKKSAW TENEDTIIY QAHKVLGNQ WAKIAKLLP 
      GRTDNAIKN HWNSTMRRR YETENRQDG ETRRGRARK 
      NQTKQADIL AFQYQNHQV VTIDNCDSR SVNQDNLII 
      NDEWTLETY DHGSNQSST GGFSLTATP SPGPLTPSH 
      TVVSFSSSP PPSTTSVKA DLPSYSYLD VNTYNNQTS 
      PVKLTPMTD DFDLKYCTP SGINPLRDF SESRHFIKS 
      RLTPPNLNQ NTLVPAENM TRASTPPIL RRGAKSRRR 
      RESEGQDIM SDCAEDRNI DEFASMLGY STKPTNNNG 
      TSPIKQLPF SPSQFFNSP GFLEMPLSS TPVKRFPTT 
      PLKDRSLKP DYSPLSTPN GLSVKVEML SSTTDPTVT 
      PSKRSFQSM DTPRTPTPF KKALADLEK KSGPITHLP 
      DTPTRLEDI TEIMKKDQD ISSNYETDT SLMITNDSG 
      YMTGKRKNG NGLAEGKEN VLPNKRVRK ALAPSWAST 
      SSQDMSSFA VETPSKSLG DDPSALFST PSSIMKDSL 
      GVTGLMDFA QSGSSKRLP SASAPSRAP GPRSTAAKR 
      ITFEDTRPK APKIEKMWA MVACGKTQD QIDLTKRAH 
      NFLRTTSLK PRSLNF
    CDS sequence
    • >TC030738 RA
      atggctcac tactaccag tctagccaa gacagatac 
      cgaaaggaa gattcggaa atcgacatt tctggaaat 
      gaggattcc gactcttca gcattcacc gaagaacct 
      caacattcc aaaacgaag aaacccata aacaagggc 
      cggtggagc aaagaggag gacgcccgt ttgaagcag 
      ctggtggag gaatacaac gagaaatgg gatgtgatt 
      gcggagctt ttcccagat aggtcggac gtccaatgc 
      cagcagcgc tggaccaaa gtggtgaat cccgaactg 
      gtcaagggc ccctggacc aaagaggaa gacgaaaaa 
      gtgatcgaa ttggtgaat aaatacgga gcgaagaaa 
      tggaccttg attgcgcgt cacctgaag ggccgtatc 
      ggcaaacag tgtcgggag cgatggcac aaccacctc 
      aaccccaaa ataaaaaag agcgcttgg acggagaac 
      gaagacacg attatctac caagcgcac aaggttttg 
      ggcaaccag tgggccaaa atcgccaaa ctgttgccc 
      ggaaggacg gacaatgcc atcaaaaac cactggaac 
      tccaccatg cggcggagg tacgagacc gagaaccgc 
      caagacggg gagacgcgc cgtgggaga gccagaaag 
      aaccaaaca aaacaagca gacattctg gcctttcag 
      taccagaac caccaagtt gtgaccatt gataattgt 
      gatagtcgc agtgtcaac caggataat ctgattatc 
      aatgatgaa tggactttg gagacgtac gaccatgga 
      agcaaccag tccagcaca ggcggtttc tctctgacg 
      gccaccccc agtccaggt cctctgacc cccagccat 
      acggtcgtc agcttttcc tcctcgcct ccaccctcc 
      acgacctca gttaaagca gatttgcca tcatacagc 
      taccttgat gtaaatacg tacaacaac caaactagt 
      cccgtgaaa ctaacacca atgactgac gattttgac 
      ctgaaatac tgtaccccg agtggaata aacccactg 
      agggatttc tccgaaagt cgccacttt atcaagtca 
      cgcctgaca ccgccgaac ctcaaccaa aacacttta 
      gtacctgct gaaaatatg acccgagcc tccactcct 
      cccatcctg cgacggggt gccaagtcg aggcgaaga 
      agggagagt gaaggccag gatatcatg tcggactgc 
      gctgaagat cggaacatc gacgaattt gcgtctatg 
      ttgggctac agcacgaaa ccaaccaac aacaatggc 
      acctccccg ataaaacag ttgccgttc agcccttca 
      cagttcttc aacagtcct ggcttttta gagatgcct 
      ttatcctca acgcccgtc aagagattc ccgacgaca 
      ccattgaaa gatcgatcg ttaaaacct gactacagt 
      cccttgagc acccccaac ggtctttca gtcaaggtg 
      gaaatgctg agttccaca accgaccca acggtgaca 
      cccagcaag cgctcgttc caaagtatg gacacgccc 
      cgtacccca acgcccttc aagaaggcc ttagccgac 
      ttggagaag aagtccggc cctataacg cacctccca 
      gacacgcca actcgtctg gaagacatc acggaaatc 
      atgaagaag gaccaagat atcagttcg aattacgaa 
      accgacacc agtctgatg attacaaat gatagtggt 
      tacatgacg gggaagaga aaaaatggg aacgggctc 
      gctgaaggc aaggaaaac gttcttccg aataagcga 
      gtgcggaag gctttggcg ccgtcttgg gcctccacc 
      tcgtcgcag gatatgtcg tcgttcgct gtggaaact 
      cccagtaaa tcgttgggc gacgatccc tcagctctg 
      ttctccacg ccgtcttcg ataatgaaa gactctttg 
      ggagtgacc ggactgatg gattttgct cagtcagga 
      tcatccaag cgcctgcca tctgccagt gccccctcc 
      cgcgcccct ggtccacga agcaccgcc gccaagcgg 
      atcactttt gaggatact aggcccaaa gctccaaag 
      atcgagaag atgtgggcc atggttgcg tgcggcaag 
      acgcaggac cagatcgac cttactaaa cgagcacac 
      aatttttta agaactacc agcttgaaa cctcgttct 
      ttaaatttt taa
    mRNA sequence
    • >TC030738 RA
      tatcctcct tggtccgcc cctgttttt gtgcccatt 
      gcgcaggtc ctcccgttg tcaaaggcg cgctgagaa 
      ccagaaacc gtccttcgt ttgcagtcc aaaagcgag 
      gtctctgag tgactgttg tcgaattaa agtaaacac 
      ggcgagtgc attgcgtga gtgctgcag ccctacggg 
      gctcccgtg tggatatgg ctcactact accagtcta 
      gccaagaca gataccgaa aggaagatt cggaaatcg 
      acatttctg gaaatgagg attccgact cttcagcat 
      tcaccgaag aacctcaac attccaaaa cgaagaaac 
      ccataaaca agggccggt ggagcaaag aggaggacg 
      cccgtttga agcagctgg tggaggaat acaacgaga 
      aatgggatg tgattgcgg agcttttcc cagataggt 
      cggacgtcc aatgccagc agcgctgga ccaaagtgg 
      tgaatcccg aactggtca agggcccct ggaccaaag 
      aggaagacg aaaaagtga tcgaattgg tgaataaat 
      acggagcga agaaatgga ccttgattg cgcgtcacc 
      tgaagggcc gtatcggca aacagtgtc gggagcgat 
      ggcacaacc acctcaacc ccaaaataa aaaagagcg 
      cttggacgg agaacgaag acacgatta tctaccaag 
      cgcacaagg ttttgggca accagtggg ccaaaatcg 
      ccaaactgt tgcccggaa ggacggaca atgccatca 
      aaaaccact ggaactcca ccatgcggc ggaggtacg 
      agaccgaga accgccaag acggggaga cgcgccgtg 
      ggagagcca gaaagaacc aaacaaaac aagcagaca 
      ttctggcct ttcagtacc agaaccacc aagttgtga 
      ccattgata attgtgata gtcgcagtg tcaaccagg 
      ataatctga ttatcaatg atgaatgga ctttggaga 
      cgtacgacc atggaagca accagtcca gcacaggcg 
      gtttctctc tgacggcca cccccagtc caggtcctc 
      tgaccccca gccatacgg tcgtcagct tttcctcct 
      cgcctccac cctccacga cctcagtta aagcagatt 
      tgccatcat acagctacc ttgatgtaa atacgtaca 
      acaaccaaa ctagtcccg tgaaactaa caccaatga 
      ctgacgatt ttgacctga aatactgta ccccgagtg 
      gaataaacc cactgaggg atttctccg aaagtcgcc 
      actttatca agtcacgcc tgacaccgc cgaacctca 
      accaaaaca ctttagtac ctgctgaaa atatgaccc 
      gagcctcca ctcctccca tcctgcgac ggggtgcca 
      agtcgaggc gaagaaggg agagtgaag gccaggata 
      tcatgtcgg actgcgctg aagatcgga acatcgacg 
      aatttgcgt ctatgttgg gctacagca cgaaaccaa 
      ccaacaaca atggcacct ccccgataa aacagttgc 
      cgttcagcc cttcacagt tcttcaaca gtcctggct 
      ttttagaga tgcctttat cctcaacgc ccgtcaaga 
      gattcccga cgacaccat tgaaagatc gatcgttaa 
      aacctgact acagtccct tgagcaccc ccaacggtc 
      tttcagtca aggtggaaa tgctgagtt ccacaaccg 
      acccaacgg tgacaccca gcaagcgct cgttccaaa 
      gtatggaca cgccccgta ccccaacgc ccttcaaga 
      aggccttag ccgacttgg agaagaagt ccggcccta 
      taacgcacc tcccagaca cgccaactc gtctggaag 
      acatcacgg aaatcatga agaaggacc aagatatca 
      gttcgaatt acgaaaccg acaccagtc tgatgatta 
      caaatgata gtggttaca tgacgggga agagaaaaa 
      atgggaacg ggctcgctg aaggcaagg aaaacgttc 
      ttccgaata agcgagtgc ggaaggctt tggcgccgt 
      cttgggcct ccacctcgt cgcaggata tgtcgtcgt 
      tcgctgtgg aaactccca gtaaatcgt tgggcgacg 
      atccctcag ctctgttct ccacgccgt cttcgataa 
      tgaaagact ctttgggag tgaccggac tgatggatt 
      ttgctcagt caggatcat ccaagcgcc tgccatctg 
      ccagtgccc cctcccgcg cccctggtc cacgaagca 
      ccgccgcca agcggatca cttttgagg atactaggc 
      ccaaagctc caaagatcg agaagatgt gggccatgg 
      ttgcgtgcg gcaagacgc aggaccaga tcgacctta 
      ctaaacgag cacacaatt ttttaagaa ctaccagct 
      tgaaacctc gttctttaa atttttaag aaaattttg 
      tacatatgc gattcggtt tacactttg taaatagaa 
      tctttttta caaataatt tagtgcaaa cgattgtat 
      tgtagattt gtcggctaa attcaataa agcatcacc 
      ggagtgagg tttcgttta cggcttcac tttacttct 
      ttttaacta cgtgtaatg tatatagac ttttcactg 
      agtaaatga aaccttact gaacaaaaa ccatgtata 
      gaactagtc taccatttt tcttgtata attgttttc 
      gtaattata tcttttaat atatacagt tattagaaa 
      ggacgccca aaggttgaa gttgtaatt ttattgcca 
      tggtaggtc taggtctct ttcttgttg tcctttggg 
      ttgtttcgc ttataaagc atgcttcag tttaatttt 
      tgcatctat ttaaagaat gtgtttctg aatttatat 
      ttattttat aattttatg tcactaaat aaaattggt 
      acttttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY