| General information | |||
| TC030738 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10001932..10015140 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030738 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06058 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC030738 RA
MAHYYQSSQ DRYRKEDSE IDISGNEDS DSSAFTEEP QHSKTKKPI NKGRWSKEE DARLKQLVE EYNEKWDVI AELFPDRSD VQCQQRWTK VVNPELVKG PWTKEEDEK VIELVNKYG AKKWTLIAR HLKGRIGKQ CRERWHNHL NPKIKKSAW TENEDTIIY QAHKVLGNQ WAKIAKLLP GRTDNAIKN HWNSTMRRR YETENRQDG ETRRGRARK NQTKQADIL AFQYQNHQV VTIDNCDSR SVNQDNLII NDEWTLETY DHGSNQSST GGFSLTATP SPGPLTPSH TVVSFSSSP PPSTTSVKA DLPSYSYLD VNTYNNQTS PVKLTPMTD DFDLKYCTP SGINPLRDF SESRHFIKS RLTPPNLNQ NTLVPAENM TRASTPPIL RRGAKSRRR RESEGQDIM SDCAEDRNI DEFASMLGY STKPTNNNG TSPIKQLPF SPSQFFNSP GFLEMPLSS TPVKRFPTT PLKDRSLKP DYSPLSTPN GLSVKVEML SSTTDPTVT PSKRSFQSM DTPRTPTPF KKALADLEK KSGPITHLP DTPTRLEDI TEIMKKDQD ISSNYETDT SLMITNDSG YMTGKRKNG NGLAEGKEN VLPNKRVRK ALAPSWAST SSQDMSSFA VETPSKSLG DDPSALFST PSSIMKDSL GVTGLMDFA QSGSSKRLP SASAPSRAP GPRSTAAKR ITFEDTRPK APKIEKMWA MVACGKTQD QIDLTKRAH NFLRTTSLK PRSLNF
- >TC030738 RA
atggctcac tactaccag tctagccaa gacagatac cgaaaggaa gattcggaa atcgacatt tctggaaat gaggattcc gactcttca gcattcacc gaagaacct caacattcc aaaacgaag aaacccata aacaagggc cggtggagc aaagaggag gacgcccgt ttgaagcag ctggtggag gaatacaac gagaaatgg gatgtgatt gcggagctt ttcccagat aggtcggac gtccaatgc cagcagcgc tggaccaaa gtggtgaat cccgaactg gtcaagggc ccctggacc aaagaggaa gacgaaaaa gtgatcgaa ttggtgaat aaatacgga gcgaagaaa tggaccttg attgcgcgt cacctgaag ggccgtatc ggcaaacag tgtcgggag cgatggcac aaccacctc aaccccaaa ataaaaaag agcgcttgg acggagaac gaagacacg attatctac caagcgcac aaggttttg ggcaaccag tgggccaaa atcgccaaa ctgttgccc ggaaggacg gacaatgcc atcaaaaac cactggaac tccaccatg cggcggagg tacgagacc gagaaccgc caagacggg gagacgcgc cgtgggaga gccagaaag aaccaaaca aaacaagca gacattctg gcctttcag taccagaac caccaagtt gtgaccatt gataattgt gatagtcgc agtgtcaac caggataat ctgattatc aatgatgaa tggactttg gagacgtac gaccatgga agcaaccag tccagcaca ggcggtttc tctctgacg gccaccccc agtccaggt cctctgacc cccagccat acggtcgtc agcttttcc tcctcgcct ccaccctcc acgacctca gttaaagca gatttgcca tcatacagc taccttgat gtaaatacg tacaacaac caaactagt cccgtgaaa ctaacacca atgactgac gattttgac ctgaaatac tgtaccccg agtggaata aacccactg agggatttc tccgaaagt cgccacttt atcaagtca cgcctgaca ccgccgaac ctcaaccaa aacacttta gtacctgct gaaaatatg acccgagcc tccactcct cccatcctg cgacggggt gccaagtcg aggcgaaga agggagagt gaaggccag gatatcatg tcggactgc gctgaagat cggaacatc gacgaattt gcgtctatg ttgggctac agcacgaaa ccaaccaac aacaatggc acctccccg ataaaacag ttgccgttc agcccttca cagttcttc aacagtcct ggcttttta gagatgcct ttatcctca acgcccgtc aagagattc ccgacgaca ccattgaaa gatcgatcg ttaaaacct gactacagt cccttgagc acccccaac ggtctttca gtcaaggtg gaaatgctg agttccaca accgaccca acggtgaca cccagcaag cgctcgttc caaagtatg gacacgccc cgtacccca acgcccttc aagaaggcc ttagccgac ttggagaag aagtccggc cctataacg cacctccca gacacgcca actcgtctg gaagacatc acggaaatc atgaagaag gaccaagat atcagttcg aattacgaa accgacacc agtctgatg attacaaat gatagtggt tacatgacg gggaagaga aaaaatggg aacgggctc gctgaaggc aaggaaaac gttcttccg aataagcga gtgcggaag gctttggcg ccgtcttgg gcctccacc tcgtcgcag gatatgtcg tcgttcgct gtggaaact cccagtaaa tcgttgggc gacgatccc tcagctctg ttctccacg ccgtcttcg ataatgaaa gactctttg ggagtgacc ggactgatg gattttgct cagtcagga tcatccaag cgcctgcca tctgccagt gccccctcc cgcgcccct ggtccacga agcaccgcc gccaagcgg atcactttt gaggatact aggcccaaa gctccaaag atcgagaag atgtgggcc atggttgcg tgcggcaag acgcaggac cagatcgac cttactaaa cgagcacac aatttttta agaactacc agcttgaaa cctcgttct ttaaatttt taa
- >TC030738 RA
tatcctcct tggtccgcc cctgttttt gtgcccatt gcgcaggtc ctcccgttg tcaaaggcg cgctgagaa ccagaaacc gtccttcgt ttgcagtcc aaaagcgag gtctctgag tgactgttg tcgaattaa agtaaacac ggcgagtgc attgcgtga gtgctgcag ccctacggg gctcccgtg tggatatgg ctcactact accagtcta gccaagaca gataccgaa aggaagatt cggaaatcg acatttctg gaaatgagg attccgact cttcagcat tcaccgaag aacctcaac attccaaaa cgaagaaac ccataaaca agggccggt ggagcaaag aggaggacg cccgtttga agcagctgg tggaggaat acaacgaga aatgggatg tgattgcgg agcttttcc cagataggt cggacgtcc aatgccagc agcgctgga ccaaagtgg tgaatcccg aactggtca agggcccct ggaccaaag aggaagacg aaaaagtga tcgaattgg tgaataaat acggagcga agaaatgga ccttgattg cgcgtcacc tgaagggcc gtatcggca aacagtgtc gggagcgat ggcacaacc acctcaacc ccaaaataa aaaagagcg cttggacgg agaacgaag acacgatta tctaccaag cgcacaagg ttttgggca accagtggg ccaaaatcg ccaaactgt tgcccggaa ggacggaca atgccatca aaaaccact ggaactcca ccatgcggc ggaggtacg agaccgaga accgccaag acggggaga cgcgccgtg ggagagcca gaaagaacc aaacaaaac aagcagaca ttctggcct ttcagtacc agaaccacc aagttgtga ccattgata attgtgata gtcgcagtg tcaaccagg ataatctga ttatcaatg atgaatgga ctttggaga cgtacgacc atggaagca accagtcca gcacaggcg gtttctctc tgacggcca cccccagtc caggtcctc tgaccccca gccatacgg tcgtcagct tttcctcct cgcctccac cctccacga cctcagtta aagcagatt tgccatcat acagctacc ttgatgtaa atacgtaca acaaccaaa ctagtcccg tgaaactaa caccaatga ctgacgatt ttgacctga aatactgta ccccgagtg gaataaacc cactgaggg atttctccg aaagtcgcc actttatca agtcacgcc tgacaccgc cgaacctca accaaaaca ctttagtac ctgctgaaa atatgaccc gagcctcca ctcctccca tcctgcgac ggggtgcca agtcgaggc gaagaaggg agagtgaag gccaggata tcatgtcgg actgcgctg aagatcgga acatcgacg aatttgcgt ctatgttgg gctacagca cgaaaccaa ccaacaaca atggcacct ccccgataa aacagttgc cgttcagcc cttcacagt tcttcaaca gtcctggct ttttagaga tgcctttat cctcaacgc ccgtcaaga gattcccga cgacaccat tgaaagatc gatcgttaa aacctgact acagtccct tgagcaccc ccaacggtc tttcagtca aggtggaaa tgctgagtt ccacaaccg acccaacgg tgacaccca gcaagcgct cgttccaaa gtatggaca cgccccgta ccccaacgc ccttcaaga aggccttag ccgacttgg agaagaagt ccggcccta taacgcacc tcccagaca cgccaactc gtctggaag acatcacgg aaatcatga agaaggacc aagatatca gttcgaatt acgaaaccg acaccagtc tgatgatta caaatgata gtggttaca tgacgggga agagaaaaa atgggaacg ggctcgctg aaggcaagg aaaacgttc ttccgaata agcgagtgc ggaaggctt tggcgccgt cttgggcct ccacctcgt cgcaggata tgtcgtcgt tcgctgtgg aaactccca gtaaatcgt tgggcgacg atccctcag ctctgttct ccacgccgt cttcgataa tgaaagact ctttgggag tgaccggac tgatggatt ttgctcagt caggatcat ccaagcgcc tgccatctg ccagtgccc cctcccgcg cccctggtc cacgaagca ccgccgcca agcggatca cttttgagg atactaggc ccaaagctc caaagatcg agaagatgt gggccatgg ttgcgtgcg gcaagacgc aggaccaga tcgacctta ctaaacgag cacacaatt ttttaagaa ctaccagct tgaaacctc gttctttaa atttttaag aaaattttg tacatatgc gattcggtt tacactttg taaatagaa tctttttta caaataatt tagtgcaaa cgattgtat tgtagattt gtcggctaa attcaataa agcatcacc ggagtgagg tttcgttta cggcttcac tttacttct ttttaacta cgtgtaatg tatatagac ttttcactg agtaaatga aaccttact gaacaaaaa ccatgtata gaactagtc taccatttt tcttgtata attgttttc gtaattata tcttttaat atatacagt tattagaaa ggacgccca aaggttgaa gttgtaatt ttattgcca tggtaggtc taggtctct ttcttgttg tcctttggg ttgtttcgc ttataaagc atgcttcag tttaatttt tgcatctat ttaaagaat gtgtttctg aatttatat ttattttat aattttatg tcactaaat aaaattggt acttttgt
