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Gene information
General information
TC030738 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10001932..10015140
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030738
    iBeetle screen iB_06058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030738 RA
      MAHYYQSSQ DRYRKEDSE IDISGNEDS DSSAFTEEP 
QHSKTKKPI NKGRWSKEE DARLKQLVE EYNEKWDVI 
AELFPDRSD VQCQQRWTK VVNPELVKG PWTKEEDEK 
VIELVNKYG AKKWTLIAR HLKGRIGKQ CRERWHNHL 
NPKIKKSAW TENEDTIIY QAHKVLGNQ WAKIAKLLP 
GRTDNAIKN HWNSTMRRR YETENRQDG ETRRGRARK 
NQTKQADIL AFQYQNHQV VTIDNCDSR SVNQDNLII 
NDEWTLETY DHGSNQSST GGFSLTATP SPGPLTPSH 
TVVSFSSSP PPSTTSVKA DLPSYSYLD VNTYNNQTS 
PVKLTPMTD DFDLKYCTP SGINPLRDF SESRHFIKS 
RLTPPNLNQ NTLVPAENM TRASTPPIL RRGAKSRRR 
RESEGQDIM SDCAEDRNI DEFASMLGY STKPTNNNG 
TSPIKQLPF SPSQFFNSP GFLEMPLSS TPVKRFPTT 
PLKDRSLKP DYSPLSTPN GLSVKVEML SSTTDPTVT 
PSKRSFQSM DTPRTPTPF KKALADLEK KSGPITHLP 
DTPTRLEDI TEIMKKDQD ISSNYETDT SLMITNDSG 
YMTGKRKNG NGLAEGKEN VLPNKRVRK ALAPSWAST 
SSQDMSSFA VETPSKSLG DDPSALFST PSSIMKDSL 
GVTGLMDFA QSGSSKRLP SASAPSRAP GPRSTAAKR 
ITFEDTRPK APKIEKMWA MVACGKTQD QIDLTKRAH 
NFLRTTSLK PRSLNF
    CDS sequence
    • >TC030738 RA
      atggctcac tactaccag tctagccaa gacagatac 
cgaaaggaa gattcggaa atcgacatt tctggaaat 
gaggattcc gactcttca gcattcacc gaagaacct 
caacattcc aaaacgaag aaacccata aacaagggc 
cggtggagc aaagaggag gacgcccgt ttgaagcag 
ctggtggag gaatacaac gagaaatgg gatgtgatt 
gcggagctt ttcccagat aggtcggac gtccaatgc 
cagcagcgc tggaccaaa gtggtgaat cccgaactg 
gtcaagggc ccctggacc aaagaggaa gacgaaaaa 
gtgatcgaa ttggtgaat aaatacgga gcgaagaaa 
tggaccttg attgcgcgt cacctgaag ggccgtatc 
ggcaaacag tgtcgggag cgatggcac aaccacctc 
aaccccaaa ataaaaaag agcgcttgg acggagaac 
gaagacacg attatctac caagcgcac aaggttttg 
ggcaaccag tgggccaaa atcgccaaa ctgttgccc 
ggaaggacg gacaatgcc atcaaaaac cactggaac 
tccaccatg cggcggagg tacgagacc gagaaccgc 
caagacggg gagacgcgc cgtgggaga gccagaaag 
aaccaaaca aaacaagca gacattctg gcctttcag 
taccagaac caccaagtt gtgaccatt gataattgt 
gatagtcgc agtgtcaac caggataat ctgattatc 
aatgatgaa tggactttg gagacgtac gaccatgga 
agcaaccag tccagcaca ggcggtttc tctctgacg 
gccaccccc agtccaggt cctctgacc cccagccat 
acggtcgtc agcttttcc tcctcgcct ccaccctcc 
acgacctca gttaaagca gatttgcca tcatacagc 
taccttgat gtaaatacg tacaacaac caaactagt 
cccgtgaaa ctaacacca atgactgac gattttgac 
ctgaaatac tgtaccccg agtggaata aacccactg 
agggatttc tccgaaagt cgccacttt atcaagtca 
cgcctgaca ccgccgaac ctcaaccaa aacacttta 
gtacctgct gaaaatatg acccgagcc tccactcct 
cccatcctg cgacggggt gccaagtcg aggcgaaga 
agggagagt gaaggccag gatatcatg tcggactgc 
gctgaagat cggaacatc gacgaattt gcgtctatg 
ttgggctac agcacgaaa ccaaccaac aacaatggc 
acctccccg ataaaacag ttgccgttc agcccttca 
cagttcttc aacagtcct ggcttttta gagatgcct 
ttatcctca acgcccgtc aagagattc ccgacgaca 
ccattgaaa gatcgatcg ttaaaacct gactacagt 
cccttgagc acccccaac ggtctttca gtcaaggtg 
gaaatgctg agttccaca accgaccca acggtgaca 
cccagcaag cgctcgttc caaagtatg gacacgccc 
cgtacccca acgcccttc aagaaggcc ttagccgac 
ttggagaag aagtccggc cctataacg cacctccca 
gacacgcca actcgtctg gaagacatc acggaaatc 
atgaagaag gaccaagat atcagttcg aattacgaa 
accgacacc agtctgatg attacaaat gatagtggt 
tacatgacg gggaagaga aaaaatggg aacgggctc 
gctgaaggc aaggaaaac gttcttccg aataagcga 
gtgcggaag gctttggcg ccgtcttgg gcctccacc 
tcgtcgcag gatatgtcg tcgttcgct gtggaaact 
cccagtaaa tcgttgggc gacgatccc tcagctctg 
ttctccacg ccgtcttcg ataatgaaa gactctttg 
ggagtgacc ggactgatg gattttgct cagtcagga 
tcatccaag cgcctgcca tctgccagt gccccctcc 
cgcgcccct ggtccacga agcaccgcc gccaagcgg 
atcactttt gaggatact aggcccaaa gctccaaag 
atcgagaag atgtgggcc atggttgcg tgcggcaag 
acgcaggac cagatcgac cttactaaa cgagcacac 
aatttttta agaactacc agcttgaaa cctcgttct 
ttaaatttt taa
    mRNA sequence
    • >TC030738 RA
      tatcctcct tggtccgcc cctgttttt gtgcccatt 
gcgcaggtc ctcccgttg tcaaaggcg cgctgagaa 
ccagaaacc gtccttcgt ttgcagtcc aaaagcgag 
gtctctgag tgactgttg tcgaattaa agtaaacac 
ggcgagtgc attgcgtga gtgctgcag ccctacggg 
gctcccgtg tggatatgg ctcactact accagtcta 
gccaagaca gataccgaa aggaagatt cggaaatcg 
acatttctg gaaatgagg attccgact cttcagcat 
tcaccgaag aacctcaac attccaaaa cgaagaaac 
ccataaaca agggccggt ggagcaaag aggaggacg 
cccgtttga agcagctgg tggaggaat acaacgaga 
aatgggatg tgattgcgg agcttttcc cagataggt 
cggacgtcc aatgccagc agcgctgga ccaaagtgg 
tgaatcccg aactggtca agggcccct ggaccaaag 
aggaagacg aaaaagtga tcgaattgg tgaataaat 
acggagcga agaaatgga ccttgattg cgcgtcacc 
tgaagggcc gtatcggca aacagtgtc gggagcgat 
ggcacaacc acctcaacc ccaaaataa aaaagagcg 
cttggacgg agaacgaag acacgatta tctaccaag 
cgcacaagg ttttgggca accagtggg ccaaaatcg 
ccaaactgt tgcccggaa ggacggaca atgccatca 
aaaaccact ggaactcca ccatgcggc ggaggtacg 
agaccgaga accgccaag acggggaga cgcgccgtg 
ggagagcca gaaagaacc aaacaaaac aagcagaca 
ttctggcct ttcagtacc agaaccacc aagttgtga 
ccattgata attgtgata gtcgcagtg tcaaccagg 
ataatctga ttatcaatg atgaatgga ctttggaga 
cgtacgacc atggaagca accagtcca gcacaggcg 
gtttctctc tgacggcca cccccagtc caggtcctc 
tgaccccca gccatacgg tcgtcagct tttcctcct 
cgcctccac cctccacga cctcagtta aagcagatt 
tgccatcat acagctacc ttgatgtaa atacgtaca 
acaaccaaa ctagtcccg tgaaactaa caccaatga 
ctgacgatt ttgacctga aatactgta ccccgagtg 
gaataaacc cactgaggg atttctccg aaagtcgcc 
actttatca agtcacgcc tgacaccgc cgaacctca 
accaaaaca ctttagtac ctgctgaaa atatgaccc 
gagcctcca ctcctccca tcctgcgac ggggtgcca 
agtcgaggc gaagaaggg agagtgaag gccaggata 
tcatgtcgg actgcgctg aagatcgga acatcgacg 
aatttgcgt ctatgttgg gctacagca cgaaaccaa 
ccaacaaca atggcacct ccccgataa aacagttgc 
cgttcagcc cttcacagt tcttcaaca gtcctggct 
ttttagaga tgcctttat cctcaacgc ccgtcaaga 
gattcccga cgacaccat tgaaagatc gatcgttaa 
aacctgact acagtccct tgagcaccc ccaacggtc 
tttcagtca aggtggaaa tgctgagtt ccacaaccg 
acccaacgg tgacaccca gcaagcgct cgttccaaa 
gtatggaca cgccccgta ccccaacgc ccttcaaga 
aggccttag ccgacttgg agaagaagt ccggcccta 
taacgcacc tcccagaca cgccaactc gtctggaag 
acatcacgg aaatcatga agaaggacc aagatatca 
gttcgaatt acgaaaccg acaccagtc tgatgatta 
caaatgata gtggttaca tgacgggga agagaaaaa 
atgggaacg ggctcgctg aaggcaagg aaaacgttc 
ttccgaata agcgagtgc ggaaggctt tggcgccgt 
cttgggcct ccacctcgt cgcaggata tgtcgtcgt 
tcgctgtgg aaactccca gtaaatcgt tgggcgacg 
atccctcag ctctgttct ccacgccgt cttcgataa 
tgaaagact ctttgggag tgaccggac tgatggatt 
ttgctcagt caggatcat ccaagcgcc tgccatctg 
ccagtgccc cctcccgcg cccctggtc cacgaagca 
ccgccgcca agcggatca cttttgagg atactaggc 
ccaaagctc caaagatcg agaagatgt gggccatgg 
ttgcgtgcg gcaagacgc aggaccaga tcgacctta 
ctaaacgag cacacaatt ttttaagaa ctaccagct 
tgaaacctc gttctttaa atttttaag aaaattttg 
tacatatgc gattcggtt tacactttg taaatagaa 
tctttttta caaataatt tagtgcaaa cgattgtat 
tgtagattt gtcggctaa attcaataa agcatcacc 
ggagtgagg tttcgttta cggcttcac tttacttct 
ttttaacta cgtgtaatg tatatagac ttttcactg 
agtaaatga aaccttact gaacaaaaa ccatgtata 
gaactagtc taccatttt tcttgtata attgttttc 
gtaattata tcttttaat atatacagt tattagaaa 
ggacgccca aaggttgaa gttgtaatt ttattgcca 
tggtaggtc taggtctct ttcttgttg tcctttggg 
ttgtttcgc ttataaagc atgcttcag tttaatttt 
tgcatctat ttaaagaat gtgtttctg aatttatat 
ttattttat aattttatg tcactaaat aaaattggt 
acttttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY