Status
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Gene information
General information
TC030733 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14912223..14914942
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030733
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06057

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030733 RA
      MSLSGNLGS PSKDTISSS GSLVGSENL PRDFSRLSL 
      FSSGSATSQ DEQFRSEGH AEHSLSCMQ NYLQAGKLC 
      DVTLVAGNN GKKVQAHRL VLSAASEYF AAMFTGNLR 
      ESGESEITL GDVNGDVLQ AVVNYCYTG AIDIREDNV 
      ETLLSTACL MQLHEVVEA CSRFLAHQL HPSNCLGIA 
      VFAEHQACT SLLQEANAY TSQNFMQVI RNQEFLQLS 
      AEQMINLLS NDDLNVTSE EHIFHALMS WINHDPVTR 
      KQMIGLLLA FVKLPLLSP EFLTDQVEP AVGSDPVCQ 
      TLIMEAFKW HLLPDRHFQ MASARTRPR KATLGRLLV 
      VGGMDKNKG ATTIESYDP RSDRWAVAH HMSGRRLQF 
      GIALLGDKL LVVGGRDGL KTLNTMECL DMETGSWTQ 
      LSPMNTHRH GLGVAVLGG TLYAVGGHD GWSYLNNVE 
      RWDPVTRSW SYVTPMQSQ RCSAGVAVL KDKLYAVGG 
      RDGASCLRT VECYDPHTN KWTMCAPLA RRRGGVGVA 
      VANGYLYAL GGQDAPANN PAASRFDCV ERYDPSTDS 
      WIVIASLSS KRDAVAACL FGDRLVAVG GYDGSHYLR 
      TVEQYDPYT NEWTALAPL ITGRAGACV IAVSNAHVQ 
      SGD
    CDS sequence
    • >TC030733 RA
      atgtcattg tctggaaat ttggggagt ccttccaaa 
      gatacaatc tcaagcagc ggaagtctc gtcggaagc 
      gaaaactta ccgagggac tttagtagg ttgtctctg 
      ttctcgtcc ggttctgcg acttctcag gatgagcag 
      ttcaggagt gaggggcac gctgaacat agtctctcc 
      tgcatgcag aattactta caagctggg aaattgtgc 
      gatgtgacg cttgtggcc ggcaataat gggaaaaag 
      gtgcaagcg cacagactc gttctttcc gctgctagt 
      gaatatttt gctgcgatg tttactggc aatttacgc 
      gaatcgggc gaatccgaa atcacactg ggtgatgtt 
      aacggcgat gttttgcaa gctgttgtg aattactgt 
      tatacgggg gcgattgat ataagggaa gacaatgta 
      gagacactt ctatcaacg gcatgtctc atgcaatta 
      cacgaagtt gtggaagct tgttcacgc tttctggcc 
      caccaactg caccccagc aattgcctg ggaattgca 
      gttttcgcc gaacatcag gcttgcact agtttgttg 
      caagaagcc aatgcttac acaagtcag aactttatg 
      caagtgata cgaaaccaa gaatttctc cagttaagt 
      gcggagcaa atgattaat ttgttatca aatgatgat 
      ttaaatgtc acgtcagag gagcacatt ttccacgct 
      ttgatgagt tggatcaac catgatcca gtcacccgg 
      aaacaaatg attggctta ctactggct tttgttaag 
      ttgccgcta ctttcgccc gaatttctg acagatcaa 
      gtggaacca gctgttggt tctgacccc gtctgtcag 
      acactaatc atggaggcg ttcaaatgg cacttgtta 
      ccagacagg cattttcaa atggctagt gctagaact 
      agaccacgt aaggcaaca cttggtagg ttactagtc 
      gtaggcggc atggataaa aacaaaggc gcaacgacg 
      atagaaagc tacgatccg aggagtgat cggtgggcg 
      gtggcacac cacatgagt gggcggaga ctacaattc 
      gggatcgca ctattgggc gataagtta ttagtcgtg 
      ggtggccgt gatggtctc aaaacgcta aacacaatg 
      gaatgtttg gatatggag actgggtct tggacccag 
      ttatcacca atgaacacc catcgacat gggctgggc 
      gtggccgtt ttgggcggg actttatac gcggtgggc 
      gggcacgac gggtggagt tatctcaat aatgttgaa 
      cggtgggac cctgttaca cgttcttgg agttatgtg 
      acccccatg caaagtcag cgttgctcg gctggggtt 
      gccgtctta aaagacaaa ctttatgca gttgggggt 
      cgtgatggg gcttcgtgt ttgcgtacg gtggaatgt 
      tacgacccc catacgaac aaatggacg atgtgtgcc 
      cccttggcc aggcggcgt gggggtgtg ggggtagct 
      gtggccaat ggctacttg tatgcttta ggggggcaa 
      gatgccccg gctaataat ccagccgcg agtagattt 
      gattgtgtt gagagatac gatcccagc actgatagt 
      tggattgtg attgcttca ctgtcgagt aaacgcgat 
      gccgtggca gcttgttta tttggtgac agacttgta 
      gctgttggg ggatatgat ggcagtcac tatttgaga 
      acagtggag cagtatgat ccctacact aatgagtgg 
      actgccctt gcaccttta attacaggt agggcaggt 
      gcttgcgta attgccgtc tcaaatgct cacgttcaa 
      tcaggtgac taa
    mRNA sequence
    • >TC030733 RA
      gctaatgac tattgttgt caaattaag attttcctg 
      ctagttgat acatgttta gcggtttac atttaactt 
      ttttgattc ccctccttg tgtgtaatt aacgtcaag 
      acgaagttt agtgatgtc attgtctgg aaatttggg 
      gagtccttc caaagatac aatctcaag cagcggaag 
      tctcgtcgg aagcgaaaa cttaccgag ggactttag 
      taggttgtc tctgttctc gtccggttc tgcgacttc 
      tcaggatga gcagttcag gagtgaggg gcacgctga 
      acatagtct ctcctgcat gcagaatta cttacaagc 
      tgggaaatt gtgcgatgt gacgcttgt ggccggcaa 
      taatgggaa aaaggtgca agcgcacag actcgttct 
      ttccgctgc tagtgaata ttttgctgc gatgtttac 
      tggcaattt acgcgaatc gggcgaatc cgaaatcac 
      actgggtga tgttaacgg cgatgtttt gcaagctgt 
      tgtgaatta ctgttatac gggggcgat tgatataag 
      ggaagacaa tgtagagac acttctatc aacggcatg 
      tctcatgca attacacga agttgtgga agcttgttc 
      acgctttct ggcccacca actgcaccc cagcaattg 
      cctgggaat tgcagtttt cgccgaaca tcaggcttg 
      cactagttt gttgcaaga agccaatgc ttacacaag 
      tcagaactt tatgcaagt gatacgaaa ccaagaatt 
      tctccagtt aagtgcgga gcaaatgat taatttgtt 
      atcaaatga tgatttaaa tgtcacgtc agaggagca 
      cattttcca cgctttgat gagttggat caaccatga 
      tccagtcac ccggaaaca aatgattgg cttactact 
      ggcttttgt taagttgcc gctactttc gcccgaatt 
      tctgacaga tcaagtgga accagctgt tggttctga 
      ccccgtctg tcagacact aatcatgga ggcgttcaa 
      atggcactt gttaccaga caggcattt tcaaatggc 
      tagtgctag aactagacc acgtaaggc aacacttgg 
      taggttact agtcgtagg cggcatgga taaaaacaa 
      aggcgcaac gacgataga aagctacga tccgaggag 
      tgatcggtg ggcggtggc acaccacat gagtgggcg 
      gagactaca attcgggat cgcactatt gggcgataa 
      gttattagt cgtgggtgg ccgtgatgg tctcaaaac 
      gctaaacac aatggaatg tttggatat ggagactgg 
      gtcttggac ccagttatc accaatgaa cacccatcg 
      acatgggct gggcgtggc cgttttggg cgggacttt 
      atacgcggt gggcgggca cgacgggtg gagttatct 
      caataatgt tgaacggtg ggaccctgt tacacgttc 
      ttggagtta tgtgacccc catgcaaag tcagcgttg 
      ctcggctgg ggttgccgt cttaaaaga caaacttta 
      tgcagttgg gggtcgtga tggggcttc gtgtttgcg 
      tacggtgga atgttacga cccccatac gaacaaatg 
      gacgatgtg tgccccctt ggccaggcg gcgtggggg 
      tgtgggggt agctgtggc caatggcta cttgtatgc 
      tttaggggg gcaagatgc cccggctaa taatccagc 
      cgcgagtag atttgattg tgttgagag atacgatcc 
      cagcactga tagttggat tgtgattgc ttcactgtc 
      gagtaaacg cgatgccgt ggcagcttg tttatttgg 
      tgacagact tgtagctgt tgggggata tgatggcag 
      tcactattt gagaacagt ggagcagta tgatcccta 
      cactaatga gtggactgc ccttgcacc tttaattac 
      aggtagggc aggtgcttg cgtaattgc cgtctcaaa 
      tgctcacgt tcaatcagg tgactaaat agtcccgat 
      taactcttt gaatatttg catttactc gtataaaca 
      ttcctaatt cgtattacg aaatctgca agataaaat 
      gttcgtact ttttctatt tcacgtgtg gattattca 
      atgtagagt agagtagtg tcacttttt atgcaatat 
      ttttacttt tatacatag ataagactt cagtattat 
      tgcaataat tttttcgga ttaatgaat gtctcattt 
      ctggtgttc ctttgtcac acaaagact gaaccgaac 
      tgaatttat ttttactat ccttccagc agtgttcac 
      attttggtg taaatatcg tgtgattga taaaattct 
      gcatcctag tgccaataa ttcactagg atgtgagac 
      tttgtaatc tcgttgttg tattcaccc atttttgca 
      ttttgtgat ggtagtttt ctgcaagga aaaattgtg 
      ctttcccaa tttaaagtt gtgtcctgt gataaaatt 
      aagaccttg cagaaatat cttgtgaga aattgtgat 
      aattataac attttgcaa ataaataaa ctttctcaa 
      t
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