General information | |||
TC030733 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 14912223..14914942 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC030733 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06057 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC030733 RA
MSLSGNLGS PSKDTISSS GSLVGSENL PRDFSRLSL FSSGSATSQ DEQFRSEGH AEHSLSCMQ NYLQAGKLC DVTLVAGNN GKKVQAHRL VLSAASEYF AAMFTGNLR ESGESEITL GDVNGDVLQ AVVNYCYTG AIDIREDNV ETLLSTACL MQLHEVVEA CSRFLAHQL HPSNCLGIA VFAEHQACT SLLQEANAY TSQNFMQVI RNQEFLQLS AEQMINLLS NDDLNVTSE EHIFHALMS WINHDPVTR KQMIGLLLA FVKLPLLSP EFLTDQVEP AVGSDPVCQ TLIMEAFKW HLLPDRHFQ MASARTRPR KATLGRLLV VGGMDKNKG ATTIESYDP RSDRWAVAH HMSGRRLQF GIALLGDKL LVVGGRDGL KTLNTMECL DMETGSWTQ LSPMNTHRH GLGVAVLGG TLYAVGGHD GWSYLNNVE RWDPVTRSW SYVTPMQSQ RCSAGVAVL KDKLYAVGG RDGASCLRT VECYDPHTN KWTMCAPLA RRRGGVGVA VANGYLYAL GGQDAPANN PAASRFDCV ERYDPSTDS WIVIASLSS KRDAVAACL FGDRLVAVG GYDGSHYLR TVEQYDPYT NEWTALAPL ITGRAGACV IAVSNAHVQ SGD
- >TC030733 RA
atgtcattg tctggaaat ttggggagt ccttccaaa gatacaatc tcaagcagc ggaagtctc gtcggaagc gaaaactta ccgagggac tttagtagg ttgtctctg ttctcgtcc ggttctgcg acttctcag gatgagcag ttcaggagt gaggggcac gctgaacat agtctctcc tgcatgcag aattactta caagctggg aaattgtgc gatgtgacg cttgtggcc ggcaataat gggaaaaag gtgcaagcg cacagactc gttctttcc gctgctagt gaatatttt gctgcgatg tttactggc aatttacgc gaatcgggc gaatccgaa atcacactg ggtgatgtt aacggcgat gttttgcaa gctgttgtg aattactgt tatacgggg gcgattgat ataagggaa gacaatgta gagacactt ctatcaacg gcatgtctc atgcaatta cacgaagtt gtggaagct tgttcacgc tttctggcc caccaactg caccccagc aattgcctg ggaattgca gttttcgcc gaacatcag gcttgcact agtttgttg caagaagcc aatgcttac acaagtcag aactttatg caagtgata cgaaaccaa gaatttctc cagttaagt gcggagcaa atgattaat ttgttatca aatgatgat ttaaatgtc acgtcagag gagcacatt ttccacgct ttgatgagt tggatcaac catgatcca gtcacccgg aaacaaatg attggctta ctactggct tttgttaag ttgccgcta ctttcgccc gaatttctg acagatcaa gtggaacca gctgttggt tctgacccc gtctgtcag acactaatc atggaggcg ttcaaatgg cacttgtta ccagacagg cattttcaa atggctagt gctagaact agaccacgt aaggcaaca cttggtagg ttactagtc gtaggcggc atggataaa aacaaaggc gcaacgacg atagaaagc tacgatccg aggagtgat cggtgggcg gtggcacac cacatgagt gggcggaga ctacaattc gggatcgca ctattgggc gataagtta ttagtcgtg ggtggccgt gatggtctc aaaacgcta aacacaatg gaatgtttg gatatggag actgggtct tggacccag ttatcacca atgaacacc catcgacat gggctgggc gtggccgtt ttgggcggg actttatac gcggtgggc gggcacgac gggtggagt tatctcaat aatgttgaa cggtgggac cctgttaca cgttcttgg agttatgtg acccccatg caaagtcag cgttgctcg gctggggtt gccgtctta aaagacaaa ctttatgca gttgggggt cgtgatggg gcttcgtgt ttgcgtacg gtggaatgt tacgacccc catacgaac aaatggacg atgtgtgcc cccttggcc aggcggcgt gggggtgtg ggggtagct gtggccaat ggctacttg tatgcttta ggggggcaa gatgccccg gctaataat ccagccgcg agtagattt gattgtgtt gagagatac gatcccagc actgatagt tggattgtg attgcttca ctgtcgagt aaacgcgat gccgtggca gcttgttta tttggtgac agacttgta gctgttggg ggatatgat ggcagtcac tatttgaga acagtggag cagtatgat ccctacact aatgagtgg actgccctt gcaccttta attacaggt agggcaggt gcttgcgta attgccgtc tcaaatgct cacgttcaa tcaggtgac taa
- >TC030733 RA
gctaatgac tattgttgt caaattaag attttcctg ctagttgat acatgttta gcggtttac atttaactt ttttgattc ccctccttg tgtgtaatt aacgtcaag acgaagttt agtgatgtc attgtctgg aaatttggg gagtccttc caaagatac aatctcaag cagcggaag tctcgtcgg aagcgaaaa cttaccgag ggactttag taggttgtc tctgttctc gtccggttc tgcgacttc tcaggatga gcagttcag gagtgaggg gcacgctga acatagtct ctcctgcat gcagaatta cttacaagc tgggaaatt gtgcgatgt gacgcttgt ggccggcaa taatgggaa aaaggtgca agcgcacag actcgttct ttccgctgc tagtgaata ttttgctgc gatgtttac tggcaattt acgcgaatc gggcgaatc cgaaatcac actgggtga tgttaacgg cgatgtttt gcaagctgt tgtgaatta ctgttatac gggggcgat tgatataag ggaagacaa tgtagagac acttctatc aacggcatg tctcatgca attacacga agttgtgga agcttgttc acgctttct ggcccacca actgcaccc cagcaattg cctgggaat tgcagtttt cgccgaaca tcaggcttg cactagttt gttgcaaga agccaatgc ttacacaag tcagaactt tatgcaagt gatacgaaa ccaagaatt tctccagtt aagtgcgga gcaaatgat taatttgtt atcaaatga tgatttaaa tgtcacgtc agaggagca cattttcca cgctttgat gagttggat caaccatga tccagtcac ccggaaaca aatgattgg cttactact ggcttttgt taagttgcc gctactttc gcccgaatt tctgacaga tcaagtgga accagctgt tggttctga ccccgtctg tcagacact aatcatgga ggcgttcaa atggcactt gttaccaga caggcattt tcaaatggc tagtgctag aactagacc acgtaaggc aacacttgg taggttact agtcgtagg cggcatgga taaaaacaa aggcgcaac gacgataga aagctacga tccgaggag tgatcggtg ggcggtggc acaccacat gagtgggcg gagactaca attcgggat cgcactatt gggcgataa gttattagt cgtgggtgg ccgtgatgg tctcaaaac gctaaacac aatggaatg tttggatat ggagactgg gtcttggac ccagttatc accaatgaa cacccatcg acatgggct gggcgtggc cgttttggg cgggacttt atacgcggt gggcgggca cgacgggtg gagttatct caataatgt tgaacggtg ggaccctgt tacacgttc ttggagtta tgtgacccc catgcaaag tcagcgttg ctcggctgg ggttgccgt cttaaaaga caaacttta tgcagttgg gggtcgtga tggggcttc gtgtttgcg tacggtgga atgttacga cccccatac gaacaaatg gacgatgtg tgccccctt ggccaggcg gcgtggggg tgtgggggt agctgtggc caatggcta cttgtatgc tttaggggg gcaagatgc cccggctaa taatccagc cgcgagtag atttgattg tgttgagag atacgatcc cagcactga tagttggat tgtgattgc ttcactgtc gagtaaacg cgatgccgt ggcagcttg tttatttgg tgacagact tgtagctgt tgggggata tgatggcag tcactattt gagaacagt ggagcagta tgatcccta cactaatga gtggactgc ccttgcacc tttaattac aggtagggc aggtgcttg cgtaattgc cgtctcaaa tgctcacgt tcaatcagg tgactaaat agtcccgat taactcttt gaatatttg catttactc gtataaaca ttcctaatt cgtattacg aaatctgca agataaaat gttcgtact ttttctatt tcacgtgtg gattattca atgtagagt agagtagtg tcacttttt atgcaatat ttttacttt tatacatag ataagactt cagtattat tgcaataat tttttcgga ttaatgaat gtctcattt ctggtgttc ctttgtcac acaaagact gaaccgaac tgaatttat ttttactat ccttccagc agtgttcac attttggtg taaatatcg tgtgattga taaaattct gcatcctag tgccaataa ttcactagg atgtgagac tttgtaatc tcgttgttg tattcaccc atttttgca ttttgtgat ggtagtttt ctgcaagga aaaattgtg ctttcccaa tttaaagtt gtgtcctgt gataaaatt aagaccttg cagaaatat cttgtgaga aattgtgat aattataac attttgcaa ataaataaa ctttctcaa t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
