Status
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Gene information
General information
TC030730 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5797502..5817302
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030730
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06056

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Many different inconsistent defects.  
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030730 RA
      MSEYDRIRR ACLKCGELW EDPDFPATQ ASVFYHQTP 
PFQFTWKRP KDLCARPGF VQDGPLQFD IIPGKMGDR 
WLVSCLGVL YLNKGLFYR VVPADQSFN GDQYAGVFR 
FRLWWCGEW TEVLVDDRL PTVHGRLAF LQSQNSDYF 
WPGLLEKAY AKLHGSYEA LKYGSLLDG LADLTGGIT 
ESIPIRQDA TTCARLLHK LLDMTSIIT CTVQPPNFQ 
MRSSVEKLP NGIQLGTNY RVYLVERVE TYSGEAVQL 
VKLRNALGP ASEYVGPWS IDSPEWSEV SPNSLERLH 
SELSEGEFW MSYPDFIKT FTHMEVIHL DSDTSRDEP 
SLHNKNTWQ MRLYQGNWL KGVSAGGCR NNPETFHIN 
PQLHLLLSE MEEVVVSLN QHSIMEPKV IGFTAYSIP 
KSNTETIGK SFFKANKSL FNSQYTNSR QVSYRCYLE 
QGGYLIIPT IFEPGQESG FTLRVYSSK PLKLKLLDT 
VPLLLKSAI VKAPPLLDG KSFSQYEAV FLQLADEHR 
TVNAFELQE LLDACLPND YIKSCASIE VCRQVILTF 
DTSGTGRLK FSDFKDLMC SLKFWQTSF KNHTKEKTG 
ILKAERFRD ALQAVGFQL SSDVLATLI LRYMRKDGT 
LRFGDFVSA ILHLTVAFN IFKMKDPLQ NGSIKLSLA 
EWLKSSLSC
    CDS sequence
    • >TC030730 RA
      atgagcgag tacgaccgc atcaggagg gcttgtcta 
aaatgtggg gagctctgg gaggatccg gattttcct 
gctacgcaa gcctcggtc ttctaccat caaactcct 
ccgtttcag ttcacgtgg aaacgaccc aaagacctc 
tgcgcgagg ccgggattc gtccaggat gggccgctc 
cagttcgac atcatccca gggaagatg ggtgacaga 
tggctcgtc tcctgtttg ggtgtttta tatttaaat 
aaaggactc ttctaccga gtcgtgcca gcagaccag 
tcttttaat ggagaccaa tacgctgga gttttccga 
tttcggctt tggtggtgc ggcgaatgg actgaggtt 
ttggtggat gatcgtttg cccactgtc catgggcgg 
ctggctttc ctgcagtcg cagaattcg gactatttc 
tggccagga ttgctcgag aaagcgtac gccaaatta 
cacgggtct tacgaagcc ttaaagtac ggttcactc 
ctcgatggt ctggcagac ctgaccggg ggaatcacc 
gaaagcatt ccaattaga caagatgcc accacatgt 
gccagactt ttacacaaa cttttagac atgacatca 
attatcacc tgcacggta caaccgccc aacttccag 
atgcgatcg agcgtggaa aagctacca aatggaata 
caactaggc accaattat cgagtatat ctagtggaa 
agggttgaa acctacagc ggtgaagcc gtccagttg 
gtgaaactg cggaacgcc ctgggcccg gcatcggaa 
tacgtgggt ccttggtcg atagactcc cccgaatgg 
tccgaagtg tctccaaac agtctcgaa aggctccac 
tcggagctt tccgaaggc gagttctgg atgtcctac 
cccgacttc atcaagacc ttcacacac atggaagtc 
atccacttg gacagcgac accagcagg gacgagccc 
agtctgcac aacaaaaac acgtggcag atgaggcta 
taccagggc aactggctc aagggagtc tcagccggg 
ggctgccgg aacaacccg gaaaccttc cacataaac 
ccccaactc cacctcctt ctcagcgag atggaggag 
gtcgtggtg tcgctcaac caacatagc ataatggag 
cccaaagtc ataggtttt acggcgtat tcaatacca 
aaaagcaac acagagacg atcgggaag tcgttcttc 
aaagccaac aagtccctc ttcaactcg caatacacc 
aacagccgg caggtcagc tacaggtgc tacctggag 
caagggggc tatctgatc atccccacc atattcgag 
cccgggcaa gagtcgggc ttcactctc agggtttac 
tcaagcaag cccttaaaa ctgaagtta ttagacact 
gtacctctg ctcctcaaa tcggcaata gtcaaagca 
ccgccgttg ctcgacggc aaaagcttc agtcaatac 
gaggccgtg ttcctgcaa ctggcggac gaacacaga 
accgtcaac gccttcgag ctgcaggag ttgctggac 
gcttgtttg ccgaacgat tacatcaaa agttgtgcc 
tcgattgaa gtttgcaga caagtcata ctcacgttt 
gatacgtcg gggacaggc agactgaag tttagtgat 
ttcaaggat ttgatgtgt agtttaaaa ttttggcaa 
acttcgttc aaaaatcac acgaaggag aagacgggg 
attttgaaa gctgagagg tttcgggat gctttacaa 
gctgttggt tttcagtta agtagtgat gttctggcc 
acgcttatt ttgaggtac atgaggaaa gacgggact 
ttacgcttc ggggatttt gtctctgcc atattacat 
ttgactgtt gctttcaat atttttaaa atgaaggac 
ccgctacaa aatggtagc atcaaatta agtctagca 
gagtggctg aagtcatct ctatcgtgc tga
    mRNA sequence
    • >TC030730 RA
      ttctgaaaa aattaattt cttgtaagt aaactgatt 
tggggtccg gttacgaac tgttattta acagttgct 
tgtttctta atttgaggt ctggcccct ccaccttga 
cggaaactg taacaattt caaagttgc atattaatg 
gttaaatga taaaagaaa gttgatacg taagttgaa 
gaatttcgc ctgatatct aaaagcgct agcggcaag 
aatgaacgg ggtcagacc tgtacgatg taattcttg 
gtcgaggta ctccgagca gttgctgaa gagctacaa 
agtggcgcg gatgaattt gttacgttt tatacgatt 
cgaacggta cacgtgctc caacttttc ataaaataa 
tctgaatga aaccttatt ttacgcagt tgatcaatt 
tgagtttta gttgattgt tggtgctgt gacctgttt 
ataatgtga ttgtggctt ttggatttt tcaaagatt 
gtgcgcgga aaactgaag gcatgagcg agtacgacc 
gcatcagga gggcttgtc taaaatgtg gggagctct 
gggaggatc cggattttc ctgctacgc aagcctcgg 
tcttctacc atcaaactc ctccgtttc agttcacgt 
ggaaacgac ccaaagacc tctgcgcga ggccgggat 
tcgtccagg atgggccgc tccagttcg acatcatcc 
cagggaaga tgggtgaca gatggctcg tctcctgtt 
tgggtgttt tatatttaa ataaaggac tcttctacc 
gagtcgtgc cagcagacc agtctttta atggagacc 
aatacgctg gagttttcc gatttcggc tttggtggt 
gcggcgaat ggactgagg ttttggtgg atgatcgtt 
tgcccactg tccatgggc ggctggctt tcctgcagt 
cgcagaatt cggactatt tctggccag gattgctcg 
agaaagcgt acgccaaat tacacgggt cttacgaag 
ccttaaagt acggttcac tcctcgatg gtctggcag 
acctgaccg ggggaatca ccgaaagca ttccaatta 
gacaagatg ccaccacat gtgccagac ttttacaca 
aacttttag acatgacat caattatca cctgcacgg 
tacaaccgc ccaacttcc agatgcgat cgagcgtgg 
aaaagctac caaatggaa tacaactag gcaccaatt 
atcgagtat atctagtgg aaagggttg aaacctaca 
gcggtgaag ccgtccagt tggtgaaac tgcggaacg 
ccctgggcc cggcatcgg aatacgtgg gtccttggt 
cgatagact cccccgaat ggtccgaag tgtctccaa 
acagtctcg aaaggctcc actcggagc tttccgaag 
gcgagttct ggatgtcct accccgact tcatcaaga 
ccttcacac acatggaag tcatccact tggacagcg 
acaccagca gggacgagc ccagtctgc acaacaaaa 
acacgtggc agatgaggc tataccagg gcaactggc 
tcaagggag tctcagccg ggggctgcc ggaacaacc 
cggaaacct tccacataa acccccaac tccacctcc 
ttctcagcg agatggagg aggtcgtgg tgtcgctca 
accaacata gcataatgg agcccaaag tcataggtt 
ttacggcgt attcaatac caaaaagca acacagaga 
cgatcggga agtcgttct tcaaagcca acaagtccc 
tcttcaact cgcaataca ccaacagcc ggcaggtca 
gctacaggt gctacctgg agcaagggg gctatctga 
tcatcccca ccatattcg agcccgggc aagagtcgg 
gcttcactc tcagggttt actcaagca agcccttaa 
aactgaagt tattagaca ctgtacctc tgctcctca 
aatcggcaa tagtcaaag caccgccgt tgctcgacg 
gcaaaagct tcagtcaat acgaggccg tgttcctgc 
aactggcgg acgaacaca gaaccgtca acgccttcg 
agctgcagg agttgctgg acgcttgtt tgccgaacg 
attacatca aaagttgtg cctcgattg aagtttgca 
gacaagtca tactcacgt ttgatacgt cggggacag 
gcagactga agtttagtg atttcaagg atttgatgt 
gtagtttaa aattttggc aaacttcgt tcaaaaatc 
acacgaagg agaagacgg ggattttga aagctgaga 
ggtttcggg atgctttac aagctgttg gttttcagt 
taagtagtg atgttctgg ccacgctta ttttgaggt 
acatgagga aagacggga ctttacgct tcggggatt 
ttgtctctg ccatattac atttgactg ttgctttca 
atattttta aaatgaagg acccgctac aaaatggta 
gcatcaaat taagtctag cagagtggc tgaagtcat 
ctctatcgt gctgatttt tcgttgctt cacatgtac 
ataaaatat atatactat gtatattat aaattaatt 
ttatgtatt ttgtaagat atcgaatag ttttaataa 
aaaatataa acaaac
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