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Gene information
General information
TC034719 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 740265..756313
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
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    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06053

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Sometimes the lateral musculature is also affected.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen ventral potentiallynot closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen dorsal potentiallynot closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment sometimes partiallyfused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC034719 RA
    CDS sequence
    • >TC034719 RA
      atgccagcc tgggggcga tttttacgg cgagtggat 
      tgcggtttc ggatgcact gataatcgt aaattccca 
      tgtcgtccg tcgtccagc agcgccctc tcatcggct 
      catcatcgg cgtgagaaa aatgtcacc atcgccatg 
      ttaaagcca agtgatcta agcgtcgcg acgacgtcc 
      gcacagcgg cgtcgtcgt cgcatcaaa aaatctatc 
      tgtgattca tctcctcat caagactgt ggacttgat 
      gaaacaaaa gaaaacgtg tttctaaca atacgtacg 
      tgcctatta actcgattc gcgtcaatt ttccaaaaa 
      acgttatta atcaagcag ttgttgctt tactatttg 
      ttactgata tcgtggtgc agtgttagt tcttcggtt 
      gaagcttat tcgagcgcg ttcgacaaa agcgaacat 
      ttactcgag aaaatgaat cttgaacag agcatcgct 
      gctgtattt aataaagta gcttacgga agcacgacg 
      aaaagatct ataccggat aatgcttat ccagttacg 
      acactcgct acaccactc cttaccacc tacaagtat 
      tccgatgga aacgacgaa tgggaacga gttgatgtg 
      aagaacgaa cggccttct ggacgaagt agtgtcgat 
      ttcgaaaca agtgactac gaagagaag aaggatgat 
      gggtatgga agcgatcta gagaaggat catgtacta 
      cctacatca gcaccagct gctgacata ttgaagaac 
      aacaacaac aaacactat aataaccct aaccggtat 
      aacaacgat cggttaaag ccggacttt gagcctggc 
      gaacaagta accgagagt aacatcaag tcaaccacg 
      ggggcaagt ctccataga aacgtacgg ccgactaaa 
      tcaatgtat agcaaagac attcccagt tatatccct 
      ccaaaccgg gattttttc acaccgcca atccctcca 
      gcattccag caccctttc gccgataaa cctaccctt 
      cgtgggaca aactcccat ggtttcgcc agtcggaga 
      cccatccca ccgcccagt ttaatgccc agcaaagac 
      cgaatcccc ttccgcccc gacctaccc aaagtcaat 
      gccgagaaa atccccgaa cccaaaacc gaaaaaacc 
      gaaaagaag aaaacgcta aacacgcca tcgcaaagt 
      gccaaattg ggcgatttc tacggcttg aatcgccac 
      aatgagacc agtttcgag tatgagaag agttacgca 
      ccggaaccg tcaatcacc aggatactg agcggttct 
      aatggacgc catgatgat atacagata ccggaaata 
      ctgtaccgt gctgtgaca gcaacacca caaaacgtc 
      aaaaccacc aagacacga gcaccggcc gcggaaccg 
      tcagttgag atcgtccgg gacgaagtc gaggagcat 
      gagactgag aggagcgag gagatcacc gaaagtgtg 
      acggtacgg aaggaggag acgacgcaa gtttcggtc 
      aacttgccg aacgttaag gagaaggag acgaacgtg 
      acaaaggcg agtaccgag gagatttgg ctgatttgt 
      tggaacgtt catgtttat ttggttgtg tttggttat 
      atcctcctg gcgatctat tcgatctat aagttggtg 
      cggtacgag aacgaccct catatgttc tcgaagagc 
      tatttcctg acgattcat ttgatgttg acggtgatt 
      tgtgtgctg aggattttc tacctcacc tatgatccg 
      tacaacgtc aataaatcg ttcaatatt ttcctatat 
      gagttcttg cattcgttc cctttgtcg cttctagca 
      acgacgttc gccgttttg atccttttc ctcctcaaa 
      cggacgctg acgcatttg gaagtcaag acgcgaccg 
      gtttttctc gtcgtgttc gcactctta catatagtt 
      ctatgtata tgtttgagc acgtttgag agtttcggc 
      cattttgaa aaggccagt ctcattgtc tgtcgaccg 
      gttttcgtc ctcctgtca tgggcgctc ggttttagt 
      tacttgtac ttgtaccga atcattaaa aacgtcttg 
      gccaagaaa agccagaac ttgtccgag atgtgcaca 
      cagaacatc tcatatgcg tcgcatata acaatcgca 
      gtggcgcta cttttcata cttttgggc ttcgtccaa 
      ttgtatgca ttgttcttc attaaaatc gaccgattc 
      aatgatgag actaaacac cattggatt ttgtggggt 
      ttcgaactt tcggtccgt ttcctcgaa atttcaata 
      atcaccctt ttggcaatc gtggtgagc ttgaaaatg 
      tcgcaacgc gagaagcaa accggggga ttccccctc 
      ttcccgtgc acgaccagc gactcaagc actgataac 
      atataccct aacaattgt aataccaac cccaacgcg 
      cttaactac agccggaac gagatgatt atggacaac 
      ataccaacc gaaccagtc gaacgtccc aatttgtta 
      aaaaccgcc tttcaaaac gactccttc gataagaaa 
      tcaacccta gaacgttac cattccgat tcggaacgc 
      actagcaat ttcgaccgt ttcgaacgc ccgaattgg 
      tccgatcgg ttcgattcg cgtccgaat aaccacgag 
      gcgaacttc ggccgaagt tcggcgcgg ttgaattcg 
      gcaaagtac tacacgaag ccgaagtat gacgatgaa 
      ggcggggcg caagaacgt aatatctat agcgaaccg 
      gtggataat caccagtac gaaaggacg cggcatgat 
      ttcgaacgc ggggatttc gatagaagg agtcggaat 
      tcgtcgaat acgtccggg aggagatcg actggtcgg 
      acgcgtaaa aaccatgct cgaccccat ctcggggtg 
      caaaccttg ccgaaccac caggattcc atgctggtg 
      gacgaacaa ggtttcgtc agatttagg cacatgcag 
      gaggagaaa ccgagtcag gggcatttt agtgatgcg 
    mRNA sequence
    • >TC034719 RA
      tcccgttgg tgtcggcga tccgaattg tccgcaacc 
      gtgtgaatt aaagcgaag gcggttgtg aatccgccc 
      ccgaggcgt cgccgcagc ccccttggg ccccacacg 
      gatgctgtt ttgtgtcag ttggtgggc agtgtgcgc 
      aaaacggcg gtgaaaaaa tgtgatgtt gtttctttg 
      tcttttatt gtcgacgat gtgaagtgt ggtcgtgtt 
      gctggtgag tttttgcgc tcgttttcg gcgttaagg 
      cggctgcag ggcggcggc ggccgctcg cgcctccgc 
      catgccagc ctgggggcg atttttacg gcgagtgga 
      ttgcggttt cggatgcac tgataatcg taaattccc 
      atgtcgtcc gtcgtccag cagcgccct ctcatcggc 
      tcatcatcg gcgtgagaa aaatgtcac catcgccat 
      gttaaagcc aagtgatct aagcgtcgc gacgacgtc 
      cgcacagcg gcgtcgtcg tcgcatcaa aaaatctat 
      ctgtgattc atctcctca tcaagactg tggacttga 
      tgaaacaaa agaaaacgt gtttctaac aatacgtac 
      gtgcctatt aactcgatt cgcgtcaat tttccaaaa 
      aacgttatt aatcaagca gttgttgct ttactattt 
      gttactgat atcgtggtg cagtgttag ttcttcggt 
      tgaagctta ttcgagcgc gttcgacaa aagcgaaca 
      tttactcga gaaaatgaa tcttgaaca gagcatcgc 
      tgctgtatt taataaagt agcttacgg aagcacgac 
      gaaaagatc tataccgga taatgctta tccagttac 
      gacactcgc tacaccact ccttaccac ctacaagta 
      ttccgatgg aaacgacga atgggaacg agttgatgt 
      gaagaacga acggccttc tggacgaag tagtgtcga 
      tttcgaaac aagtgacta cgaagagaa gaaggatga 
      tgggtatgg aagcgatct agagaagga tcatgtact 
      acctacatc agcaccagc tgctgacat attgaagaa 
      caacaacaa caaacacta taataaccc taaccggta 
      taacaacga tcggttaaa gccggactt tgagcctgg 
      cgaacaagt aaccgagag taacatcaa gtcaaccac 
      gggggcaag tctccatag aaacgtacg gccgactaa 
      atcaatgta tagcaaaga cattcccag ttatatccc 
      tccaaaccg ggatttttt cacaccgcc aatccctcc 
      agcattcca gcacccttt cgccgataa acctaccct 
      tcgtgggac aaactccca tggtttcgc cagtcggag 
      acccatccc accgcccag tttaatgcc cagcaaaga 
      ccgaatccc cttccgccc cgacctacc caaagtcaa 
      tgccgagaa aatccccga acccaaaac cgaaaaaac 
      cgaaaagaa gaaaacgct aaacacgcc atcgcaaag 
      tgccaaatt gggcgattt ctacggctt gaatcgcca 
      caatgagac cagtttcga gtatgagaa gagttacgc 
      accggaacc gtcaatcac caggatact gagcggttc 
      taatggacg ccatgatga tatacagat accggaaat 
      actgtaccg tgctgtgac agcaacacc acaaaacgt 
      caaaaccac caagacacg agcaccggc cgcggaacc 
      gtcagttga gatcgtccg ggacgaagt cgaggagca 
      tgagactga gaggagcga ggagatcac cgaaagtgt 
      gacggtacg gaaggagga gacgacgca agtttcggt 
      caacttgcc gaacgttaa ggagaagga gacgaacgt 
      gacaaaggc gagtaccga ggagatttg gctgatttg 
      ttggaacgt tcatgttta tttggttgt gtttggtta 
      tatcctcct ggcgatcta ttcgatcta taagttggt 
      gcggtacga gaacgaccc tcatatgtt ctcgaagag 
      ctatttcct gacgattca tttgatgtt gacggtgat 
      ttgtgtgct gaggatttt ctacctcac ctatgatcc 
      gtacaacgt caataaatc gttcaatat tttcctata 
      tgagttctt gcattcgtt ccctttgtc gcttctagc 
      aacgacgtt cgccgtttt gatcctttt cctcctcaa 
      acggacgct gacgcattt ggaagtcaa gacgcgacc 
      ggtttttct cgtcgtgtt cgcactctt acatatagt 
      tctatgtat atgtttgag cacgtttga gagtttcgg 
      ccattttga aaaggccag tctcattgt ctgtcgacc 
      ggttttcgt cctcctgtc atgggcgct cggttttag 
      ttacttgta cttgtaccg aatcattaa aaacgtctt 
      ggccaagaa aagccagaa cttgtccga gatgtgcac 
      acagaacat ctcatatgc gtcgcatat aacaatcgc 
      agtggcgct acttttcat acttttggg cttcgtcca 
      attgtatgc attgttctt cattaaaat cgaccgatt 
      caatgatga gactaaaca ccattggat tttgtgggg 
      tttcgaact ttcggtccg tttcctcga aatttcaat 
      aatcaccct tttggcaat cgtggtgag cttgaaaat 
      gtcgcaacg cgagaagca aaccggggg attccccct 
      cttcccgtg cacgaccag cgactcaag cactgataa 
      catataccc taacaattg taataccaa ccccaacgc 
      gcttaacta cagccggaa cgagatgat tatggacaa 
      cataccaac cgaaccagt cgaacgtcc caatttgtt 
      aaaaaccgc ctttcaaaa cgactcctt cgataagaa 
      atcaaccct agaacgtta ccattccga ttcggaacg 
      cactagcaa tttcgaccg tttcgaacg cccgaattg 
      gtccgatcg gttcgattc gcgtccgaa taaccacga 
      ggcgaactt cggccgaag ttcggcgcg gttgaattc 
      ggcaaagta ctacacgaa gccgaagta tgacgatga 
      aggcggggc gcaagaacg taatatcta tagcgaacc 
      ggtggataa tcaccagta cgaaaggac gcggcatga 
      tttcgaacg cggggattt cgatagaag gagtcggaa 
      ttcgtcgaa tacgtccgg gaggagatc gactggtcg 
      gacgcgtaa aaaccatgc tcgacccca tctcggggt 
      gcaaacctt gccgaacca ccaggattc catgctggt 
      ggacgaaca aggtttcgt cagatttag gcacatgca 
      ggaggagaa accgagtca ggggcattt tagtgatgc 
      gtgaaattc ggggctgag tcggactaa agggtagtg 
      agccgaggg ggcgaattg caattagaa aaaaagtac 
      aaatttcga tacattttg atgattttt ggagttgtc 
      agtgcaaaa aatggatgc cgggttaaa ttgggctgt 
      ggcgcccaa cgtggggcc acgaccaac taaccaaat 
      ctttgagag aaaacgagt tttaagctg caattatgg 
      ttaaacaca ctcatgatg gcatgtcta cttaatttt 
      attgaagaa atttggagt gaaacactt gaaagacat 
      taaacaacg acctaacta tacctagtt atttattga 
      taatactca tttcgtgac gtttggaag cgcagggta 
      ctagatagc cccgtttcg tgtgatttt agcaataat 
      tccgttaat tagagctca ctacccccg ccgcccccg 
      atagacggt tcggcctaa tcaaaaccc actttcgct 
      tttgacgtt ttaaaaaac ttagccatg acagtcaca 
      cgtgtgaac gcggtccct cacgaggac aaacctaat 
      aaagtaaaa atccactaa ttgttagat tatagtgta 
      tgtacgatt ataatactt tacgatgat aaagtagca 
      gtgtataaa aatgaattt gcaaaagtg tacatattt 
      ataatgtta agtgactat atataattt taatttttt 
      atataattt ttgataaga tacagggtg ttcgtgttg 
      acaacaaac aaaaagaac ggcagctac tatgacccg 
      attttctca aaactcttc tccgaattg tttcgtccc 
      ctgtatatt tttagttag tttagatga acggactga 
      attcatgtt atttattat cgttcgggc taaatacac 
      gagaaatta ccgtcctaa tgaccaaaa taggcggcg 
      gaaaaatcg ctcgccaga gggcgtctt ttccggcga 
      cgtgtcaca aatatgtag atgtgtttt gtttgtata 
      tatgtatct aagccaata tttagcctt gccattttt 
      aactgctgg gttttttaa taacgtagt aagagttaa 
      ggaagaaga attattatt tttgtacat tatgttcag 
      tattatttt tttacgttc acagtattt attattagg 
      tttcctttc tcacaaaag acttgattt ttatacatt 
      cctcgttac ttgccactc tttacttta taatgttcc 
      taagtgacg tacttatac cagtaattc tgacgtttt 
      ttgccagta tttctataa cgcctctac ttgtattag 
      tatagagag aataacatt ttttgattt ttggtgtca 
      cacaaatgt tattttaaa caaataaac tacaagata 
      taagtgaat ctggtgatt ttccaaatg tacaaaact 
      gatgaaaaa tacatttcg taaagtcat cgctttatt 
      tatacgaat cgacttcta ttgtattat attattgta 
      aaatataaa ctatgtcta agc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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