Status
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Gene information
General information
TC033855
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030711 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13736549..13748119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033855
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    leg musculature shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva bristle shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva bristle sometimes not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva seta not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva potentially anteroposterior-axis shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: bad quality of preparation,
    Embedding medium became insufficient despite a big supply in the preparation.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033855 RA
      MLPTILVTF ATVLLELCT TSPVYNMQP AFITANIGT 
VGRNIRNLP CISRKTNQS GVCMFAIDC LKANGTHLG 
TCIDRFYFG SCCHIEPVN DIIDNTIDD IIPAREPPQ 
RLSSTIKPI PTTTKTEKP PPTKTTTTT PATTTTRKV 
FITNITTTT TTIKPTTTT EKSVPLKQG ESTTVGYKL 
TTFQSVDIN IPVETTSPK TTPKSKPTK VTPKPTKTT 
PKPVRTTIS TKKPTSKPV LNTTKKPVL TTVTTRQTS 
TTVTTTTRR PSTTAVRRP STTTTRKPP TLSTRRPST 
TTTRKPTTT TKPTKPSTT TTKRPVDTK PTTRPVKIT 
STTTTRKPS TTTVTKKPS TTTTTKKPS TATTTTPKP 
TTLVTRNVT QASESNEIE TESSSSVKP PSTTAGSIV 
TWTIGNTEK NVSAPTTSS PGNSTSEEW LPLTTPDQW 
VPLPSVQAS TQTASPSTT AKPSTTESA SKPSITYIP 
LESTTSSVT LNASTVVTE GVVTSQATP ASFTPPSTT 
VGSDTEIRP VNMSDFTDV CGRRMYPEG RIVGGEKSS 
FGKWPWQIS LRQWRTSTY LHKCGAALL NENWAITAA 
HCVDNVPPS DLLLRLGEH DLSTESEPY LHQERRVQI 
VASHPQFDP RTFEYDLAL LRFYEPVTF QPNILPVCV 
PQSDENFVG RTAYVTGWG RLYEDGPLP SVLQEVSVP 
VINNSVCES MYRSAGYIE HIPHIFICA GWRRGGFDS 
CEGDSGGPM VIQREDKRF LLAGIISWG IGCAEPNQP 
GVYTRISEF RDWINQILQ F
    CDS sequence
    • >TC033855 RA
      atgctgcca acaatttta gttactttt gccactgtc 
ctgttggag ttgtgtacg acttcccct gtatataac 
atgcaaccg gcgttcatc actgccaat ataggtact 
gtaggaaga aatattcga aatctaccc tgcatttcg 
agaaaaacc aaccaaagt ggtgtttgc atgtttgct 
atagactgt ctaaaagca aacgggaca catttagga 
acttgtata gatcggttc tatttcggc tcgtgttgt 
cacatagaa ccagttaat gatatcata gataacacg 
atagatgat ataatccca gcacgagaa ccaccacaa 
agactttcg agtactatc aaacccata cctacaaca 
accaaaact gaaaaacca ccaccaacc aaaacaaca 
accacgaca cctgcgacc acaacaacg agaaaagtt 
tttattaca aacatcacc actacaaca acaacaata 
aaaccaaca acaacaacg gaaaaatcg gtaccttta 
aaacaaggt gaaagtact acagttggt tataaacta 
accacgttc caatcagtt gacataaac attcctgtt 
gaaacaaca agtcctaaa acgaccccc aaatctaaa 
ccaaccaaa gtaacccca aaaccgaca aaaactacc 
cctaaacct gttcgaact acaattagt acgaaaaag 
ccaactagc aaaccggta ctaaacacg acaaaaaaa 
ccggtgcta accacagtt acaacgcgg cagactagt 
acaacggta acaacaacg acacgtaga cctagtaca 
acagctgta cggagacca agtacaaca acaacaagg 
aaacctccg acactttca actcgtagg ccttccaca 
actactact cgtaaaccc acaacaaca acaaaacca 
accaaacct agtactact acaactaaa cgacctgtt 
gatactaaa ccaacaaca agacccgtg aaaattact 
agcacaacc acgacgaga aaacctagt actacaaca 
gtgacgaaa aaacctagt actacaacc acgacgaaa 
aaacctagc actgcaaca accacaact cccaaaccc 
acgacttta gtaacaaga aacgtgact caagcctct 
gaaagcaac gaaatcgaa accgagtct tcctcaagc 
gtgaaacca ccaagtacc accgccggt tcaatagtc 
acttggacc atcggtaac accgagaaa aatgtttcg 
gcaccgacg acttccagt cccggtaac agtacaagt 
gaagaatgg ctccctttg acgacgccc gaccagtgg 
gtgccactg cccagcgtc caggccagc acccagaca 
gcgtctcct tccacgacc gctaaacca tcgacgact 
gaaagtgca agtaaaccg tccatcacg tatatacca 
ttggaatct acaacctct tcagtgacc ttgaatgcg 
tcgactgtg gtcactgaa ggagttgtt acatcgcaa 
gcgacacct gccagcttt actcctcct tcgacgacg 
gtggggtcc gacacggaa attcgaccg gtcaacatg 
agcgacttc acggatgtt tgcgggcga aggatgtat 
cctgaaggg aggatagtt ggaggagaa aaatcgtct 
ttcggaaaa tggccatgg cagatctcc ttgaggcaa 
tggaggact tccacatac ctccacaag tgtggggct 
gccctcctc aacgagaac tgggctata accgctgct 
cactgcgtc gataacgtc ccacctagt gacctactt 
ctgcgactg ggcgaacac gacctatct acagaatcg 
gagccctac ttgcaccag gaacggcgc gtgcagatt 
gttgcatcg cacccccag tttgacccc aggaccttc 
gaatatgac ctagccctt ttgcggttc tacgagcct 
gtcactttc cagccgaat atcttgcca gtctgtgtc 
cctcaatca gacgagaac tttgtgggc aggacagcc 
tacgtcaca ggatggggg cgtttatac gaggacggt 
cccttgccg agcgttctc caggaagtg tccgtccct 
gtaatcaac aattcagtg tgtgaatca atgtaccgc 
tccgccggc tacatcgag cacatcccc cacattttt 
atctgcgcg ggatggaga aggggtggt ttcgattcg 
tgcgaaggc gactcggga ggacccatg gtgattcag 
cgcgaggac aagagattc ctccttgca ggaatcatc 
tcctgggga attggatgt gccgagccg aaccagccc 
ggagtctat accagaatc agcgaattc agagactgg 
atcaaccaa attttacag ttttaa
    mRNA sequence
    • >TC033855 RA
      cggggagtt catcgaccc ccatggtct tcaggatgg 
cagaactct tctttaacc agtcccaac tcaacgtcg 
gacaggtga tgaacgtca tggggatgg tgtccaacc 
actagcgcg cgcatattt aaaatttta aaagaaaca 
tcacgagtt ttagtaata tgactaaaa gtcgcttca 
gttaactag taccaacta tttttaaaa ttctaaaga 
atttttgca atatgtcgg tgtgattca gtgctaaag 
tgttaacta atttatttt tattattag tagagttta 
ttgaactgt ttgtatcct ctagtctta aataagatg 
ctgccaaca attttagtt acttttgcc actgtcctg 
ttggagttg tgtacgact tcccctgta tataacatg 
caaccggcg ttcatcact gccaatata ggtactgta 
ggaagaaat attcgaaat ctaccctgc atttcgaga 
aaaaccaac caaagtggt gtttgcatg tttgctata 
gactgtcta aaagcaaac gggacacat ttaggaact 
tgtatagat cggttctat ttcggctcg tgttgtcac 
atagaacca gttaatgat atcatagat aacacgata 
gatgatata atcccagca cgagaacca ccacaaaga 
ctttcgagt actatcaaa cccatacct acaacaacc 
aaaactgaa aaaccacca ccaaccaaa acaacaacc 
acgacacct gcgaccaca acaacgaga aaagttttt 
attacaaac atcaccact acaacaaca acaataaaa 
ccaacaaca acaacggaa aaatcggta cctttaaaa 
caaggtgaa agtactaca gttggttat aaactaacc 
acgttccaa tcagttgac ataaacatt cctgttgaa 
acaacaagt cctaaaacg acccccaaa tctaaacca 
accaaagta accccaaaa ccgacaaaa actacccct 
aaacctgtt cgaactaca attagtacg aaaaagcca 
actagcaaa ccggtacta aacacgaca aaaaaaccg 
gtgctaacc acagttaca acgcggcag actagtaca 
acggtaaca acaacgaca cgtagacct agtacaaca 
gctgtacgg agaccaagt acaacaaca acaaggaaa 
cctccgaca ctttcaact cgtaggcct tccacaact 
actactcgt aaacccaca acaacaaca aaaccaacc 
aaacctagt actactaca actaaacga cctgttgat 
actaaacca acaacaaga cccgtgaaa attactagc 
acaaccacg acgagaaaa cctagtact acaacagtg 
acgaaaaaa cctagtact acaaccacg acgaaaaaa 
cctagcact gcaacaacc acaactccc aaacccacg 
actttagta acaagaaac gtgactcaa gcctctgaa 
agcaacgaa atcgaaacc gagtcttcc tcaagcgtg 
aaaccacca agtaccacc gccggttca atagtcact 
tggaccatc ggtaacacc gagaaaaat gtttcggca 
ccgacgact tccagtccc ggtaacagt acaagtgaa 
gaatggctc cctttgacg acgcccgac cagtgggtg 
ccactgccc agcgtccag gccagcacc cagacagcg 
tctccttcc acgaccgct aaaccatcg acgactgaa 
agtgcaagt aaaccgtcc atcacgtat ataccattg 
gaatctaca acctcttca gtgaccttg aatgcgtcg 
actgtggtc actgaagga gttgttaca tcgcaagcg 
acacctgcc agctttact cctccttcg acgacggtg 
gggtccgac acggaaatt cgaccggtc aacatgagc 
gacttcacg gatgtttgc gggcgaagg atgtatcct 
gaagggagg atagttgga ggagaaaaa tcgtctttc 
ggaaaatgg ccatggcag atctccttg aggcaatgg 
aggacttcc acatacctc cacaagtgt ggggctgcc 
ctcctcaac gagaactgg gctataacc gctgctcac 
tgcgtcgat aacgtccca cctagtgac ctacttctg 
cgactgggc gaacacgac ctatctaca gaatcggag 
ccctacttg caccaggaa cggcgcgtg cagattgtt 
gcatcgcac ccccagttt gaccccagg accttcgaa 
tatgaccta gcccttttg cggttctac gagcctgtc 
actttccag ccgaatatc ttgccagtc tgtgtccct 
caatcagac gagaacttt gtgggcagg acagcctac 
gtcacagga tgggggcgt ttatacgag gacggtccc 
ttgccgagc gttctccag gaagtgtcc gtccctgta 
atcaacaat tcagtgtgt gaatcaatg taccgctcc 
gccggctac atcgagcac atcccccac atttttatc 
tgcgcggga tggagaagg ggtggtttc gattcgtgc 
gaaggcgac tcgggagga cccatggtg attcagcgc 
gaggacaag agattcctc cttgcagga atcatctcc 
tggggaatt ggatgtgcc gagccgaac cagcccgga 
gtctatacc agaatcagc gaattcaga gactggatc 
aaccaaatt ttacagttt taatgtgat ctgagagta 
gctaagccg aagacaacg aaagtggag agaaaagtg 
cgggttaat gcaaattgg ttgatattg attttcttg 
cactgctat aaataaatt ctgcatgca acagttaca 
gcgtagaaa gtttcagaa ttttacaca ctttactaa 
tgcaatatt ttgtgatgt ggagacttt tacaaaaga 
taaaagcgt aggtttagg acaaagcga ttgtaaaaa 
ataaaaaat atccagtaa tattactat tatgaaata 
acttattca aacattgat aaaaagagt taagaatgt 
atggacata caagttaac ttttttggt cgatcacac 
ctgtaatta tagaaagta cggacgata ttgttattt 
ttatattaa tgtaaatag aggtgcgtg attttaatt 
ttgtactgt aaatgaata aaatattta tttattt
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