Status
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Gene information
General information
TC033855
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030711
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13736549..13748119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033855
    iBeetle screen iB_06051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    leg musculature shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva bristle shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva bristle sometimes not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva seta not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva potentially anteroposterior-axis shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: bad quality of preparation,
    Embedding medium became insufficient despite a big supply in the preparation.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033855 RA
      MLPTILVTF ATVLLELCT TSPVYNMQP AFITANIGT 
      VGRNIRNLP CISRKTNQS GVCMFAIDC LKANGTHLG 
      TCIDRFYFG SCCHIEPVN DIIDNTIDD IIPAREPPQ 
      RLSSTIKPI PTTTKTEKP PPTKTTTTT PATTTTRKV 
      FITNITTTT TTIKPTTTT EKSVPLKQG ESTTVGYKL 
      TTFQSVDIN IPVETTSPK TTPKSKPTK VTPKPTKTT 
      PKPVRTTIS TKKPTSKPV LNTTKKPVL TTVTTRQTS 
      TTVTTTTRR PSTTAVRRP STTTTRKPP TLSTRRPST 
      TTTRKPTTT TKPTKPSTT TTKRPVDTK PTTRPVKIT 
      STTTTRKPS TTTVTKKPS TTTTTKKPS TATTTTPKP 
      TTLVTRNVT QASESNEIE TESSSSVKP PSTTAGSIV 
      TWTIGNTEK NVSAPTTSS PGNSTSEEW LPLTTPDQW 
      VPLPSVQAS TQTASPSTT AKPSTTESA SKPSITYIP 
      LESTTSSVT LNASTVVTE GVVTSQATP ASFTPPSTT 
      VGSDTEIRP VNMSDFTDV CGRRMYPEG RIVGGEKSS 
      FGKWPWQIS LRQWRTSTY LHKCGAALL NENWAITAA 
      HCVDNVPPS DLLLRLGEH DLSTESEPY LHQERRVQI 
      VASHPQFDP RTFEYDLAL LRFYEPVTF QPNILPVCV 
      PQSDENFVG RTAYVTGWG RLYEDGPLP SVLQEVSVP 
      VINNSVCES MYRSAGYIE HIPHIFICA GWRRGGFDS 
      CEGDSGGPM VIQREDKRF LLAGIISWG IGCAEPNQP 
      GVYTRISEF RDWINQILQ F
    CDS sequence
    • >TC033855 RA
      atgctgcca acaatttta gttactttt gccactgtc 
      ctgttggag ttgtgtacg acttcccct gtatataac 
      atgcaaccg gcgttcatc actgccaat ataggtact 
      gtaggaaga aatattcga aatctaccc tgcatttcg 
      agaaaaacc aaccaaagt ggtgtttgc atgtttgct 
      atagactgt ctaaaagca aacgggaca catttagga 
      acttgtata gatcggttc tatttcggc tcgtgttgt 
      cacatagaa ccagttaat gatatcata gataacacg 
      atagatgat ataatccca gcacgagaa ccaccacaa 
      agactttcg agtactatc aaacccata cctacaaca 
      accaaaact gaaaaacca ccaccaacc aaaacaaca 
      accacgaca cctgcgacc acaacaacg agaaaagtt 
      tttattaca aacatcacc actacaaca acaacaata 
      aaaccaaca acaacaacg gaaaaatcg gtaccttta 
      aaacaaggt gaaagtact acagttggt tataaacta 
      accacgttc caatcagtt gacataaac attcctgtt 
      gaaacaaca agtcctaaa acgaccccc aaatctaaa 
      ccaaccaaa gtaacccca aaaccgaca aaaactacc 
      cctaaacct gttcgaact acaattagt acgaaaaag 
      ccaactagc aaaccggta ctaaacacg acaaaaaaa 
      ccggtgcta accacagtt acaacgcgg cagactagt 
      acaacggta acaacaacg acacgtaga cctagtaca 
      acagctgta cggagacca agtacaaca acaacaagg 
      aaacctccg acactttca actcgtagg ccttccaca 
      actactact cgtaaaccc acaacaaca acaaaacca 
      accaaacct agtactact acaactaaa cgacctgtt 
      gatactaaa ccaacaaca agacccgtg aaaattact 
      agcacaacc acgacgaga aaacctagt actacaaca 
      gtgacgaaa aaacctagt actacaacc acgacgaaa 
      aaacctagc actgcaaca accacaact cccaaaccc 
      acgacttta gtaacaaga aacgtgact caagcctct 
      gaaagcaac gaaatcgaa accgagtct tcctcaagc 
      gtgaaacca ccaagtacc accgccggt tcaatagtc 
      acttggacc atcggtaac accgagaaa aatgtttcg 
      gcaccgacg acttccagt cccggtaac agtacaagt 
      gaagaatgg ctccctttg acgacgccc gaccagtgg 
      gtgccactg cccagcgtc caggccagc acccagaca 
      gcgtctcct tccacgacc gctaaacca tcgacgact 
      gaaagtgca agtaaaccg tccatcacg tatatacca 
      ttggaatct acaacctct tcagtgacc ttgaatgcg 
      tcgactgtg gtcactgaa ggagttgtt acatcgcaa 
      gcgacacct gccagcttt actcctcct tcgacgacg 
      gtggggtcc gacacggaa attcgaccg gtcaacatg 
      agcgacttc acggatgtt tgcgggcga aggatgtat 
      cctgaaggg aggatagtt ggaggagaa aaatcgtct 
      ttcggaaaa tggccatgg cagatctcc ttgaggcaa 
      tggaggact tccacatac ctccacaag tgtggggct 
      gccctcctc aacgagaac tgggctata accgctgct 
      cactgcgtc gataacgtc ccacctagt gacctactt 
      ctgcgactg ggcgaacac gacctatct acagaatcg 
      gagccctac ttgcaccag gaacggcgc gtgcagatt 
      gttgcatcg cacccccag tttgacccc aggaccttc 
      gaatatgac ctagccctt ttgcggttc tacgagcct 
      gtcactttc cagccgaat atcttgcca gtctgtgtc 
      cctcaatca gacgagaac tttgtgggc aggacagcc 
      tacgtcaca ggatggggg cgtttatac gaggacggt 
      cccttgccg agcgttctc caggaagtg tccgtccct 
      gtaatcaac aattcagtg tgtgaatca atgtaccgc 
      tccgccggc tacatcgag cacatcccc cacattttt 
      atctgcgcg ggatggaga aggggtggt ttcgattcg 
      tgcgaaggc gactcggga ggacccatg gtgattcag 
      cgcgaggac aagagattc ctccttgca ggaatcatc 
      tcctgggga attggatgt gccgagccg aaccagccc 
      ggagtctat accagaatc agcgaattc agagactgg 
      atcaaccaa attttacag ttttaa
    mRNA sequence
    • >TC033855 RA
      cggggagtt catcgaccc ccatggtct tcaggatgg 
      cagaactct tctttaacc agtcccaac tcaacgtcg 
      gacaggtga tgaacgtca tggggatgg tgtccaacc 
      actagcgcg cgcatattt aaaatttta aaagaaaca 
      tcacgagtt ttagtaata tgactaaaa gtcgcttca 
      gttaactag taccaacta tttttaaaa ttctaaaga 
      atttttgca atatgtcgg tgtgattca gtgctaaag 
      tgttaacta atttatttt tattattag tagagttta 
      ttgaactgt ttgtatcct ctagtctta aataagatg 
      ctgccaaca attttagtt acttttgcc actgtcctg 
      ttggagttg tgtacgact tcccctgta tataacatg 
      caaccggcg ttcatcact gccaatata ggtactgta 
      ggaagaaat attcgaaat ctaccctgc atttcgaga 
      aaaaccaac caaagtggt gtttgcatg tttgctata 
      gactgtcta aaagcaaac gggacacat ttaggaact 
      tgtatagat cggttctat ttcggctcg tgttgtcac 
      atagaacca gttaatgat atcatagat aacacgata 
      gatgatata atcccagca cgagaacca ccacaaaga 
      ctttcgagt actatcaaa cccatacct acaacaacc 
      aaaactgaa aaaccacca ccaaccaaa acaacaacc 
      acgacacct gcgaccaca acaacgaga aaagttttt 
      attacaaac atcaccact acaacaaca acaataaaa 
      ccaacaaca acaacggaa aaatcggta cctttaaaa 
      caaggtgaa agtactaca gttggttat aaactaacc 
      acgttccaa tcagttgac ataaacatt cctgttgaa 
      acaacaagt cctaaaacg acccccaaa tctaaacca 
      accaaagta accccaaaa ccgacaaaa actacccct 
      aaacctgtt cgaactaca attagtacg aaaaagcca 
      actagcaaa ccggtacta aacacgaca aaaaaaccg 
      gtgctaacc acagttaca acgcggcag actagtaca 
      acggtaaca acaacgaca cgtagacct agtacaaca 
      gctgtacgg agaccaagt acaacaaca acaaggaaa 
      cctccgaca ctttcaact cgtaggcct tccacaact 
      actactcgt aaacccaca acaacaaca aaaccaacc 
      aaacctagt actactaca actaaacga cctgttgat 
      actaaacca acaacaaga cccgtgaaa attactagc 
      acaaccacg acgagaaaa cctagtact acaacagtg 
      acgaaaaaa cctagtact acaaccacg acgaaaaaa 
      cctagcact gcaacaacc acaactccc aaacccacg 
      actttagta acaagaaac gtgactcaa gcctctgaa 
      agcaacgaa atcgaaacc gagtcttcc tcaagcgtg 
      aaaccacca agtaccacc gccggttca atagtcact 
      tggaccatc ggtaacacc gagaaaaat gtttcggca 
      ccgacgact tccagtccc ggtaacagt acaagtgaa 
      gaatggctc cctttgacg acgcccgac cagtgggtg 
      ccactgccc agcgtccag gccagcacc cagacagcg 
      tctccttcc acgaccgct aaaccatcg acgactgaa 
      agtgcaagt aaaccgtcc atcacgtat ataccattg 
      gaatctaca acctcttca gtgaccttg aatgcgtcg 
      actgtggtc actgaagga gttgttaca tcgcaagcg 
      acacctgcc agctttact cctccttcg acgacggtg 
      gggtccgac acggaaatt cgaccggtc aacatgagc 
      gacttcacg gatgtttgc gggcgaagg atgtatcct 
      gaagggagg atagttgga ggagaaaaa tcgtctttc 
      ggaaaatgg ccatggcag atctccttg aggcaatgg 
      aggacttcc acatacctc cacaagtgt ggggctgcc 
      ctcctcaac gagaactgg gctataacc gctgctcac 
      tgcgtcgat aacgtccca cctagtgac ctacttctg 
      cgactgggc gaacacgac ctatctaca gaatcggag 
      ccctacttg caccaggaa cggcgcgtg cagattgtt 
      gcatcgcac ccccagttt gaccccagg accttcgaa 
      tatgaccta gcccttttg cggttctac gagcctgtc 
      actttccag ccgaatatc ttgccagtc tgtgtccct 
      caatcagac gagaacttt gtgggcagg acagcctac 
      gtcacagga tgggggcgt ttatacgag gacggtccc 
      ttgccgagc gttctccag gaagtgtcc gtccctgta 
      atcaacaat tcagtgtgt gaatcaatg taccgctcc 
      gccggctac atcgagcac atcccccac atttttatc 
      tgcgcggga tggagaagg ggtggtttc gattcgtgc 
      gaaggcgac tcgggagga cccatggtg attcagcgc 
      gaggacaag agattcctc cttgcagga atcatctcc 
      tggggaatt ggatgtgcc gagccgaac cagcccgga 
      gtctatacc agaatcagc gaattcaga gactggatc 
      aaccaaatt ttacagttt taatgtgat ctgagagta 
      gctaagccg aagacaacg aaagtggag agaaaagtg 
      cgggttaat gcaaattgg ttgatattg attttcttg 
      cactgctat aaataaatt ctgcatgca acagttaca 
      gcgtagaaa gtttcagaa ttttacaca ctttactaa 
      tgcaatatt ttgtgatgt ggagacttt tacaaaaga 
      taaaagcgt aggtttagg acaaagcga ttgtaaaaa 
      ataaaaaat atccagtaa tattactat tatgaaata 
      acttattca aacattgat aaaaagagt taagaatgt 
      atggacata caagttaac ttttttggt cgatcacac 
      ctgtaatta tagaaagta cggacgata ttgttattt 
      ttatattaa tgtaaatag aggtgcgtg attttaatt 
      ttgtactgt aaatgaata aaatattta tttattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY