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Gene information
General information
TC030668 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10124342..10125863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030668
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06046

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030668 RA
      MSHRLLVLF FIIATIGGT LGYQNWRRD RQPKIVGDK 
      IPLNAIMPN RRKMQRKIV QYHNYFRTK VVPRAANML 
      RMRWHKGAA RAAQKWSRK CFVLTHDNI TGRHIENYG 
      ACGQNIFIA SDKVPWLFA IKTWYLEKD NFTFGSRKN 
      DLMIVGHYT QMVWASTHE VGCGLSKCY LKSSGKTFY 
      NYICNYCPI GNQPGQLGR PYRRGRPCS LCRNNCFAR 
      KLCTNSCHV ADLWANCKE LYKVWPQWL CDTTSVKGQ 
      ERKKHCRAT CTCKNKIHD
    CDS sequence
    • >TC030668 RA
      atgtcccac cgtctcctg gtactattt tttataatc 
      gccacaatt ggtggtact ttagggtac cagaactgg 
      cgccgagac agacagcct aaaatagtc ggtgataaa 
      atacccctc aatgcaatt atgccaaat cggcgaaaa 
      atgcaaaga aaaattgtc cagtaccac aattatttt 
      cgcacaaaa gtggtacca cgggctgct aatatgtta 
      cgaatgaga tggcacaaa ggtgccgcc cgagctgcc 
      caaaagtgg tctcgtaaa tgttttgtt ttgacccat 
      gataacata actgggcga catatcgaa aactatggt 
      gcttgtggt caaaacata tttatcgcc tctgacaaa 
      gtaccatgg ttatttgcc atcaaaacc tggtatttg 
      gaaaaagat aacttcacc tttggtagt cgcaaaaat 
      gacctaatg atcgtgggc cactacacc cagatggta 
      tgggcgagc acccacgaa gtcggctgc ggcttatct 
      aaatgttac ttaaaatcg agcggaaaa accttctac 
      aactacatc tgcaactac tgccccata ggaaatcag 
      ccaggacaa ctgggacgt ccttatcgc agaggaagg 
      ccttgtagt ctatgtcgg aacaactgc tttgccaga 
      aaactctgc accaattca tgccatgtg gccgacttg 
      tgggccaat tgcaaggaa ttgtacaaa gtctggccc 
      cagtggctt tgcgacacg acttcagtc aaaggccaa 
      gaaaggaaa aagcactgt agagctact tgtacttgt 
      aaaaacaaa atccacgac taa
    mRNA sequence
    • >TC030668 RA
      ttaaaattg tgacaataa aaaataatt ctaaccctc 
      gagatcaat gtactacaa acaatgaaa tgacgttat 
      ctcacatcc atgtaccaa cacgacttt ggtgatttt 
      tttgattcg agtgactga acaacaata gtgcaaaaa 
      tctaacaaa tacatgcaa ggttgactg accccaaac 
      cacaacatg tcccaccgt ctcctggta ctatttttt 
      ataatcgcc acaattggt ggtacttta gggtaccag 
      aactggcgc cgagacaga cagcctaaa atagtcggt 
      gataaaata cccctcaat gcaattatg ccaaatcgg 
      cgaaaaatg caaagaaaa attgtccag taccacaat 
      tattttcgc acaaaagtg gtaccacgg gctgctaat 
      atgttacga atgagatgg cacaaaggt gccgcccga 
      gctgcccaa aagtggtct cgtaaatgt tttgttttg 
      acccatgat aacataact gggcgacat atcgaaaac 
      tatggtgct tgtggtcaa aacatattt atcgcctct 
      gacaaagta ccatggtta tttgccatc aaaacctgg 
      tatttggaa aaagataac ttcaccttt ggtagtcgc 
      aaaaatgac ctaatgatc gtgggccac tacacccag 
      atggtatgg gcgagcacc cacgaagtc ggctgcggc 
      ttatctaaa tgttactta aaatcgagc ggaaaaacc 
      ttctacaac tacatctgc aactactgc cccatagga 
      aatcagcca ggacaactg ggacgtcct tatcgcaga 
      ggaaggcct tgtagtcta tgtcggaac aactgcttt 
      gccagaaaa ctctgcacc aattcatgc catgtggcc 
      gacttgtgg gccaattgc aaggaattg tacaaagtc 
      tggccccag tggctttgc gacacgact tcagtcaaa 
      ggccaagaa aggaaaaag cactgtaga gctacttgt 
      acttgtaaa aacaaaatc cacgactaa taaataatt 
      ggaaatcct tgttgtttt tgaaatcgt gaaagaaag 
      agaaagtgt gaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY