Status
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Gene information
General information
TC030661 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12705292..12709755
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030661
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06045

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 33.33% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030661 RA
      MDINALGWM GLIITLFLT SFGLFSFLT TALGLLTFI 
      LGFITLVYL RKGDIDKFH NHCAGNPLT VNILPEGGL 
      RQVVRQLVS PQKVQKTDS RVTGSELID NSLQEILGY 
      IVRDYISPW YNLISRDTE FTDITIRKT AQTFAINIS 
      NRVKEIDWI PYLTTRLVD DAATHLRLF KQARARMKF 
      LEKSKTPKQ SPLKDQKNS PRKTHKRNK SETDVGWYH 
      GKANDIKKE PEVNVGNSK FYLNDSKKS TTLEDHFFD 
      LECQMENYL VCRDVVSMD VAKEKEFLC EIIEILLYI 
      LLPDEDFHC KPLRYVLRE IFANCVIQP LFAMLSDPD 
      YINQAIIWL CLRDGTLPS DIFLTTLRI TDNCDELKS 
      TKNLAEKEV QQLRSRDSG GDSDLAIKQ QLSSLSYVL 
      KLIESRLIK MQDAENLDT PTSLDYVQL ESIKKIDLK 
      LHQILKNNV ALSYFIDYV SSQKKHLDL FFYLTIEGW 
      KVSVEQQLS DLHINKIKG ASDNSSAVF EEIRSTALS 
      IYNQYLGEK SDQRVVIKP ALTQSLFFK IRNLNEIPS 
      ELWFDEVQQ AIYDKMEND DEYLPAFKR SKAYFKLLQ 
      ELDLVQQNV TEEDAISLN SNESIELIN TESKSEQST 
      PQQHANNLF LVPESTGKN VKHARSFSD VTMFMSKNN 
      SEVRANGND FAKFETEEV LTVEDQRVL EKSLKTGEF 
      VLNVNIIET GIVCEKGKM FGIYAIRVS RQYETGFLE 
      EWHIYRRYS DFYDLYSKV KDKFPDLSK LSFPGKKTF 
      HNMDRAVLE RRMKMLGSY MSELCNPSV LTTHSGLQD 
      LLMMFLEQG DYDKATGGP ISSTINTLV NPLKSGMKT 
      IKNMPEQLF NTVDEVMGG ITKVFHAKP GRLPEASKV 
      GASIEETDD NIPLRIMLL LMDEVFDLK SRNQWLRRR 
      IVTLLRQIV RTMFGDIVN RRILDYVSF ITSPKNVAH 
      YLYVFKQSF WPNGMKYEK KADRDEDTR NRTRVAAKV 
      ALLSCLSDE LKHIIGSET TRRGLLTIF ELFQRPILN 
      RRLFYVLLE GVLCTLFPE KDMFKLFQK LHSKSKRIQ 
      DKHNTQTVR
    CDS sequence
    • >TC030661 RA
      atggatata aacgcttta ggatggatg ggtttaata 
      attactttg ttcttaact tctttcggg ttgttttcg 
      tttcttaca acggcccta ggactactg acttttatc 
      ctagggttc attactttg gtatattta aggaagggt 
      gatattgac aagttccac aaccactgt gctgggaac 
      cccctcaca gttaatatt ctcccagaa ggtggttta 
      agacaggtg gttaggcaa cttgtaagt cctcaaaaa 
      gtacaaaaa accgacagt agggtaaca ggcagtgag 
      ctaatcgac aactcactg caagagata ttaggctac 
      atagtcaga gactacatc tcaccatgg tacaacttg 
      atatcaaga gacaccgaa tttactgat attacgata 
      aggaaaact gcacaaact tttgctatc aacatttca 
      aacagggtg aaagagatt gactggatc ccgtattta 
      accacacgt ttggtcgat gatgcggcc acacatttg 
      cggcttttt aagcaagcc agagctcgg atgaaattt 
      ctagaaaag agtaaaaca ccaaaacag tcaccgctc 
      aaagaccag aaaaactcg ccgagaaaa actcacaag 
      aggaacaag agcgagacg gacgtgggc tggtaccat 
      ggcaaagcc aatgatatt aagaaagaa cctgaggtg 
      aatgtcgga aattcaaag ttttatctg aacgattcc 
      aagaaaagc accactttg gaagaccac ttctttgat 
      ctagagtgt caaatggaa aattactta gtttgtaga 
      gacgtagtt agtatggac gtggccaaa gaaaaagaa 
      ttcttgtgc gaaattatt gaaatatta ttatacatt 
      cttctccct gatgaagat ttccattgt aagcccttg 
      cggtacgtc ttgcgtgag atcttcgcc aactgtgtg 
      attcaaccg ctttttgct atgttgtct gatcccgat 
      tacattaat caggcgata atttggctg tgtttgcgt 
      gatggtact ttaccaagt gacattttt ttaaccact 
      ttgagaatt acagacaac tgtgatgaa ctaaaaagt 
      actaaaaac ctagctgag aaagaagta caacaattg 
      agatcacgc gattctggg ggcgacagt gatctcgca 
      atcaagcag caactgagc agtctgtct tacgttttg 
      aaattaatc gagagtcga ctaataaaa atgcaagat 
      gccgaaaat ttagacact ccaacttcc cttgattac 
      gtccaattg gagagtatt aaaaaaatc gacttaaag 
      ctgcaccag atactgaaa aataacgtc gctttgtcg 
      tactttatt gactacgtg tcgagccaa aaaaagcac 
      ctggattta tttttttac ttaaccatt gaaggctgg 
      aaggtttca gtggagcag cagttgtcc gatttgcac 
      ataaacaag atcaaaggc gccagtgac aactcatct 
      gctgtgttt gaggaaatc cgttccacc gccttgagc 
      atttacaac cagtacctg ggcgaaaaa tccgaccaa 
      cgtgtcgta attaaacca gctttgacc caaagtctc 
      tttttcaaa attcgcaat ttgaacgaa attccctcc 
      gaattgtgg tttgacgaa gtccaacaa gcgatttac 
      gacaaaatg gaaaacgat gacgagtat ttaccagca 
      tttaagagg agcaaagcg tactttaaa ctcctccaa 
      gagttggat ttggtgcaa cagaacgtg accgaagaa 
      gacgcgatt agtctgaac agcaatgaa tcgatcgaa 
      cttataaat acggagagc aaatcggag caaagtacg 
      ccacaacag cacgcaaat aatttgttt ttggtgccg 
      gaaagcacc gggaaaaac gtcaagcat gccaggtcg 
      ttcagcgat gtgacgatg tttatgagc aagaataac 
      agcgaagta agggcgaat ggcaacgac tttgcaaag 
      tttgaaacg gaggaggtt ttgacggtg gaggaccaa 
      cgcgtgctg gagaagagc ctcaagact ggagagttt 
      gtccttaac gttaatatt atcgaaaca gggattgtg 
      tgtgagaag ggcaaaatg ttcggcatt tacgcgatt 
      cgagtgagc cgccaatac gagaccggc tttctggaa 
      gagtggcac atctaccgg cgctatagc gatttttac 
      gatttgtac agcaaagtc aaggataaa tttccggat 
      ctttcaaaa ctatcattc ccgggcaaa aaaacattc 
      cataacatg gacagagcc gtccttgag cgtcgcatg 
      aaaatgcta ggctcttac atgagcgaa ttatgtaac 
      cccagtgtg ctaacgact cactccgga ttgcaagac 
      ttgttgatg atgttcctg gagcaaggg gattacgat 
      aaagcgact gggggcccg atttccagc actatcaat 
      actttagtg aatccgttg aaatccggc atgaaaacg 
      atcaaaaac atgccggaa cagttgttt aacactgtt 
      gatgaggtc atggggggc attactaaa gtttttcat 
      gccaagcct ggacgccta cctgaggct agtaaagtt 
      ggggcttca attgaagag actgatgat aacattcca 
      ctgaggatt atgttgctg cttatggac gaagttttt 
      gatttaaag tcacgaaat caatggcta aggaggaga 
      atagtgact ttgttgagg caaattgtg cggaccatg 
      ttcggtgat attgtcaat cgtaggata ttggactat 
      gtttcgttt attacaagt ccgaaaaac gtagcgcat 
      tatttatat gttttcaag caatctttt tggccaaat 
      ggtatgaaa tatgagaag aaagcggat cgagacgaa 
      gatacgcgc aatcgcacg agagtggcc gccaaagtt 
      gctctcctg tcctgccta tcagatgaa ctcaagcat 
      ataatagga tccgagacc acacggaga ggcttatta 
      acgattttc gaactgttt caaagacca attttgaac 
      cgtaggcta ttttacgtc ctgctggag ggcgttttg 
      tgtactttg tttccagaa aaagacatg ttcaaacta 
      tttcaaaaa ctccattct aagtcaaag cgaattcaa 
      gataaacac aatactcaa actgttagg tga
    mRNA sequence
    • >TC030661 RA
      acctcaaag ttgtgacat ttggcaaat cgtaaatat 
      gaagaaatt taatagtga tttaattgt tcacaataa 
      ttatgtgat ttattataa ataggatta agtcggtca 
      cacgttatc tcgtaaaag ctgcgattt attttattt 
      cctcgaacg aaaaatgga tataaacgc tttaggatg 
      gatgggttt aataattac tttgttctt aacttcttt 
      cgggttgtt ttcgtttct tacaacggc cctaggact 
      actgacttt tatcctagg gttcattac tttggtata 
      tttaaggaa gggtgatat tgacaagtt ccacaacca 
      ctgtgctgg gaaccccct cacagttaa tattctccc 
      agaaggtgg tttaagaca ggtggttag gcaacttgt 
      aagtcctca aaaagtaca aaaaaccga cagtagggt 
      aacaggcag tgagctaat cgacaactc actgcaaga 
      gatattagg ctacatagt cagagacta catctcacc 
      atggtacaa cttgatatc aagagacac cgaatttac 
      tgatattac gataaggaa aactgcaca aacttttgc 
      tatcaacat ttcaaacag ggtgaaaga gattgactg 
      gatcccgta tttaaccac acgtttggt cgatgatgc 
      ggccacaca tttgcggct ttttaagca agccagagc 
      tcggatgaa atttctaga aaagagtaa aacaccaaa 
      acagtcacc gctcaaaga ccagaaaaa ctcgccgag 
      aaaaactca caagaggaa caagagcga gacggacgt 
      gggctggta ccatggcaa agccaatga tattaagaa 
      agaacctga ggtgaatgt cggaaattc aaagtttta 
      tctgaacga ttccaagaa aagcaccac tttggaaga 
      ccacttctt tgatctaga gtgtcaaat ggaaaatta 
      cttagtttg tagagacgt agttagtat ggacgtggc 
      caaagaaaa agaattctt gtgcgaaat tattgaaat 
      attattata cattcttct ccctgatga agatttcca 
      ttgtaagcc cttgcggta cgtcttgcg tgagatctt 
      cgccaactg tgtgattca accgctttt tgctatgtt 
      gtctgatcc cgattacat taatcaggc gataatttg 
      gctgtgttt gcgtgatgg tactttacc aagtgacat 
      ttttttaac cactttgag aattacaga caactgtga 
      tgaactaaa aagtactaa aaacctagc tgagaaaga 
      agtacaaca attgagatc acgcgattc tgggggcga 
      cagtgatct cgcaatcaa gcagcaact gagcagtct 
      gtcttacgt tttgaaatt aatcgagag tcgactaat 
      aaaaatgca agatgccga aaatttaga cactccaac 
      ttcccttga ttacgtcca attggagag tattaaaaa 
      aatcgactt aaagctgca ccagatact gaaaaataa 
      cgtcgcttt gtcgtactt tattgacta cgtgtcgag 
      ccaaaaaaa gcacctgga tttattttt ttacttaac 
      cattgaagg ctggaaggt ttcagtgga gcagcagtt 
      gtccgattt gcacataaa caagatcaa aggcgccag 
      tgacaactc atctgctgt gtttgagga aatccgttc 
      caccgcctt gagcattta caaccagta cctgggcga 
      aaaatccga ccaacgtgt cgtaattaa accagcttt 
      gacccaaag tctcttttt caaaattcg caatttgaa 
      cgaaattcc ctccgaatt gtggtttga cgaagtcca 
      acaagcgat ttacgacaa aatggaaaa cgatgacga 
      gtatttacc agcatttaa gaggagcaa agcgtactt 
      taaactcct ccaagagtt ggatttggt gcaacagaa 
      cgtgaccga agaagacgc gattagtct gaacagcaa 
      tgaatcgat cgaacttat aaatacgga gagcaaatc 
      ggagcaaag tacgccaca acagcacgc aaataattt 
      gtttttggt gccggaaag caccgggaa aaacgtcaa 
      gcatgccag gtcgttcag cgatgtgac gatgtttat 
      gagcaagaa taacagcga agtaagggc gaatggcaa 
      cgactttgc aaagtttga aacggagga ggttttgac 
      ggtggagga ccaacgcgt gctggagaa gagcctcaa 
      gactggaga gtttgtcct taacgttaa tattatcga 
      aacagggat tgtgtgtga gaagggcaa aatgttcgg 
      catttacgc gattcgagt gagccgcca atacgagac 
      cggctttct ggaagagtg gcacatcta ccggcgcta 
      tagcgattt ttacgattt gtacagcaa agtcaagga 
      taaatttcc ggatctttc aaaactatc attcccggg 
      caaaaaaac attccataa catggacag agccgtcct 
      tgagcgtcg catgaaaat gctaggctc ttacatgag 
      cgaattatg taaccccag tgtgctaac gactcactc 
      cggattgca agacttgtt gatgatgtt cctggagca 
      aggggatta cgataaagc gactggggg cccgatttc 
      cagcactat caatacttt agtgaatcc gttgaaatc 
      cggcatgaa aacgatcaa aaacatgcc ggaacagtt 
      gtttaacac tgttgatga ggtcatggg gggcattac 
      taaagtttt tcatgccaa gcctggacg cctacctga 
      ggctagtaa agttggggc ttcaattga agagactga 
      tgataacat tccactgag gattatgtt gctgcttat 
      ggacgaagt ttttgattt aaagtcacg aaatcaatg 
      gctaaggag gagaatagt gactttgtt gaggcaaat 
      tgtgcggac catgttcgg tgatattgt caatcgtag 
      gatattgga ctatgtttc gtttattac aagtccgaa 
      aaacgtagc gcattattt atatgtttt caagcaatc 
      tttttggcc aaatggtat gaaatatga gaagaaagc 
      ggatcgaga cgaagatac gcgcaatcg cacgagagt 
      ggccgccaa agttgctct cctgtcctg cctatcaga 
      tgaactcaa gcatataat aggatccga gaccacacg 
      gagaggctt attaacgat tttcgaact gtttcaaag 
      accaatttt gaaccgtag gctatttta cgtcctgct 
      ggagggcgt tttgtgtac tttgtttcc agaaaaaga 
      catgttcaa actatttca aaaactcca ttctaagtc 
      aaagcgaat tcaagataa acacaatac tcaaactgt 
      taggtgata gcttattta tttgtaaat gtatcgggt 
      gccatcgcg ttctcttca ttgtattat ttattcaaa 
      gagtttgtt tatttttat agctggttt ctatccttt 
      aactgtgct taacttgtt gtattattt gttagaata 
      aagatttaa attattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY