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Gene information
General information
TC030648 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18630549..18639974
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030648
    iBeetle screen iB_06044

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030648 RA
      MAHFPSFDD KTILKTPQP VHSSSMAES DKKISTFDS 
      SFEMDNSLG ILSIKSGDL SPHEGSAAF LLQQQINLA 
      KDCINKHKE QPPVTSIPS RYLRPSEQS ANSIPFNDF 
      SSTINESDL LGNSSTPRA SACTPDLMR DRCSIGKTI 
      TALASSFAN PSFDVDKIF NTIHKTMEE CSFKSGEID 
      VAKLLEEDE KSWRREKAI PTETKDFSV TSDDFKMSI 
      GSFFQKRSD ELPELLAKS PDKREAPIP LIETSTRSE 
      ALRNSTLVK DETVSLSLI QKMLSSEES PMSALDNIL 
      NKAKMEKKY KACPEPTKA RTSPPIKLP TCQEEKEIE 
      KDTPVEPVI PTEPFNGLE EIKENISPF DGDRTAVSS 
      RSSSSLSSL PNGKLPIAT TKTEVIWGC TKPGKNSLR 
      EFVVRNRSA SRVGITCTV SNPSFRLHK DGSELNFTS 
      VLKVILHPY ESRPLCVTF APTAVGAVV DNITFTPID 
      PSHVQTTKQ FIKLYGYGG YADVVVREI VKDTTGKFL 
      CPLGSVDDQ ELKRGFFLR NNGTLPAFA HFNFTLNGF 
      YSFSNVEVE PLRAVLMPG EEREVTVKC NLSKKDLTN 
      IKSKTSCVV FEMGKLLLV HGAEVDRAR LRKLYQKAK 
      EREQQVKPW IVELVEKIH GEVVPKDLK YFRESLLSI 
      EDVVQLLNK EEITLTVEQ EADRTVTLQ HLQDETAAY 
      LSLCEQTEI FDCTTCPET CQVEPSKIF LTLPEKVKD 
      IIFVTNRNK KTIYFETNV VPEGLKLKP HDGTIKPGE 
      TVMIEVSCK QLCKDKTAF KVRILVDNE SFSVDVKVV 
      YLNA
    CDS sequence
    • >TC030648 RA
      atggctcat tttcccagc ttcgacgat aaaacaatt 
      ttaaaaacc cctcaaccg gttcattcc tctagcatg 
      gctgaaagc gataagaaa atttcaact tttgactct 
      tccttcgag atggacaat tcacttggg attttatcg 
      atcaaaagt ggtgacttg tcgccccac gaaggctcc 
      gctgccttt ttactccaa cagcaaatt aacctagcc 
      aaagactgc atcaacaaa cataaggaa cagccccct 
      gtcacgtct ataccctcc agatactta agaccaagc 
      gaacagtcg gcgaattca attcccttc aatgatttt 
      tcaagcaca atcaacgaa agcgacttg ctggggaac 
      agttccacg cctcgggcc tccgcctgc actcccgac 
      ctcatgcga gatcgctgc tcaatcggg aaaaccatc 
      actgcgctc gcaagttcg tttgcaaat ccaagtttt 
      gacgttgat aaaattttc aacacgata cacaaaacc 
      atggaggaa tgttcgttc aaatcgggg gaaatcgat 
      gtagcgaaa ttgttagaa gaggacgag aaatcgtgg 
      cggcgagaa aaggcaatt ccaaccgaa acgaaggat 
      ttttcagtc acttctgat gattttaaa atgtcaatt 
      gggagtttc ttccaaaag aggtccgat gagcttccg 
      gaattgcta gcgaaaagt cccgacaaa cgtgaggca 
      ccgatccct ttgattgaa acttcaacc agaagtgaa 
      gctttacga aatagcacg cttgtcaaa gacgagact 
      gttagtctc agtttgatt cagaaaatg ctgtcgtca 
      gaggaatct ccgatgtca gcacttgat aatatactt 
      aacaaagca aagatggag aagaagtac aaagcgtgt 
      ccagagcct actaaagct cgtaccagt ccgcccatt 
      aaactgcca acgtgtcaa gaagaaaaa gaaattgag 
      aaagacaca cctgtagaa cctgtgata ccgactgaa 
      ccattcaat ggattggaa gaaattaaa gaaaatatc 
      tcccccttc gacggagac agaacagcg gtttcttcg 
      cgaagctcg agctccttg agcagcctc cctaacggt 
      aagctcccc atagctacg accaaaacc gaagtcatt 
      tggggctgc acaaaaccc gggaagaac agtctgcgt 
      gaattcgtc gtgcgaaac agatcagca agtcgggtt 
      ggtatcact tgcaccgtt tctaatcca tcatttcgc 
      ctccacaaa gacggctcc gaacttaat tttacaagt 
      gtacttaaa gtgatttta catccgtat gaatcgcgc 
      cccctttgt gtcactttc gcccccact gccgtgggg 
      gctgtcgta gacaacatt actttcaca ccaatcgat 
      ccaagtcat gtgcaaaca actaagcag ttcatcaaa 
      ttgtacggt tatgggggc tatgctgat gtggtggtg 
      cgcgaaatc gtgaaagac accacgggg aaatttttg 
      tgtccgttg gggagtgtc gatgatcag gaattgaaa 
      cgcgggttt ttcttgaga aataatgga actttgccg 
      gcttttgcc cactttaat ttcacacta aatgggttt 
      tactcgttt tccaatgtt gaggtggag cctttgagg 
      gctgttttg atgccgggt gaggaacgg gaagtgacc 
      gttaagtgt aacctttcg aaaaaagat ctaactaat 
      atcaagtct aaaacttcg tgcgttgtt ttcgaaatg 
      gggaaacta ttgctagtg catggggca gaagtggac 
      cgcgcccgt ttgaggaaa ttgtatcag aaggcgaag 
      gaaagagag cagcaagtt aagccttgg attgttgaa 
      ctggtcgag aaaattcat ggagaagtg gttccgaaa 
      gatttgaaa tattttaga gagtcgctt ttatctatt 
      gaagacgtg gtgcagttg ttaaataag gaagaaatc 
      actctaact gttgaacag gaggctgac cgaactgtc 
      acactacaa caccttcaa gatgaaaca gcagcgtat 
      cttagttta tgtgaacag accgaaatt tttgattgt 
      acaacatgt ccagaaacg tgtcaggtc gaacccagt 
      aagattttc ttaactctt ccggaaaaa gtcaaggat 
      attattttc gtaaccaat cgtaataag aaaactatt 
      tattttgag actaatgtt gtcccagaa gggctcaaa 
      ttgaagcca catgatggt actataaag ccaggagaa 
      accgtcatg attgaggtg tcctgcaaa caattgtgt 
      aaagataag actgcgttt aaagtgaga attcttgta 
      gataacgag agttttagt gttgatgtg aaagttgtt 
      tatctcaat gcctag
    mRNA sequence
    • >TC030648 RA
      cgatcgttt atgtcaact gtttttgtc aaatttaaa 
      atcgaacta gtatttttt gttatagtg tgttttatt 
      taacttaac tgtgaattg tgattggat taaaacaaa 
      atggctcat tttcccagc ttcgacgat aaaacaatt 
      ttaaaaacc cctcaaccg gttcattcc tctagcatg 
      gctgaaagc gataagaaa atttcaact tttgactct 
      tccttcgag atggacaat tcacttggg attttatcg 
      atcaaaagt ggtgacttg tcgccccac gaaggctcc 
      gctgccttt ttactccaa cagcaaatt aacctagcc 
      aaagactgc atcaacaaa cataaggaa cagccccct 
      gtcacgtct ataccctcc agatactta agaccaagc 
      gaacagtcg gcgaattca attcccttc aatgatttt 
      tcaagcaca atcaacgaa agcgacttg ctggggaac 
      agttccacg cctcgggcc tccgcctgc actcccgac 
      ctcatgcga gatcgctgc tcaatcggg aaaaccatc 
      actgcgctc gcaagttcg tttgcaaat ccaagtttt 
      gacgttgat aaaattttc aacacgata cacaaaacc 
      atggaggaa tgttcgttc aaatcgggg gaaatcgat 
      gtagcgaaa ttgttagaa gaggacgag aaatcgtgg 
      cggcgagaa aaggcaatt ccaaccgaa acgaaggat 
      ttttcagtc acttctgat gattttaaa atgtcaatt 
      gggagtttc ttccaaaag aggtccgat gagcttccg 
      gaattgcta gcgaaaagt cccgacaaa cgtgaggca 
      ccgatccct ttgattgaa acttcaacc agaagtgaa 
      gctttacga aatagcacg cttgtcaaa gacgagact 
      gttagtctc agtttgatt cagaaaatg ctgtcgtca 
      gaggaatct ccgatgtca gcacttgat aatatactt 
      aacaaagca aagatggag aagaagtac aaagcgtgt 
      ccagagcct actaaagct cgtaccagt ccgcccatt 
      aaactgcca acgtgtcaa gaagaaaaa gaaattgag 
      aaagacaca cctgtagaa cctgtgata ccgactgaa 
      ccattcaat ggattggaa gaaattaaa gaaaatatc 
      tcccccttc gacggagac agaacagcg gtttcttcg 
      cgaagctcg agctccttg agcagcctc cctaacggt 
      aagctcccc atagctacg accaaaacc gaagtcatt 
      tggggctgc acaaaaccc gggaagaac agtctgcgt 
      gaattcgtc gtgcgaaac agatcagca agtcgggtt 
      ggtatcact tgcaccgtt tctaatcca tcatttcgc 
      ctccacaaa gacggctcc gaacttaat tttacaagt 
      gtacttaaa gtgatttta catccgtat gaatcgcgc 
      cccctttgt gtcactttc gcccccact gccgtgggg 
      gctgtcgta gacaacatt actttcaca ccaatcgat 
      ccaagtcat gtgcaaaca actaagcag ttcatcaaa 
      ttgtacggt tatgggggc tatgctgat gtggtggtg 
      cgcgaaatc gtgaaagac accacgggg aaatttttg 
      tgtccgttg gggagtgtc gatgatcag gaattgaaa 
      cgcgggttt ttcttgaga aataatgga actttgccg 
      gcttttgcc cactttaat ttcacacta aatgggttt 
      tactcgttt tccaatgtt gaggtggag cctttgagg 
      gctgttttg atgccgggt gaggaacgg gaagtgacc 
      gttaagtgt aacctttcg aaaaaagat ctaactaat 
      atcaagtct aaaacttcg tgcgttgtt ttcgaaatg 
      gggaaacta ttgctagtg catggggca gaagtggac 
      cgcgcccgt ttgaggaaa ttgtatcag aaggcgaag 
      gaaagagag cagcaagtt aagccttgg attgttgaa 
      ctggtcgag aaaattcat ggagaagtg gttccgaaa 
      gatttgaaa tattttaga gagtcgctt ttatctatt 
      gaagacgtg gtgcagttg ttaaataag gaagaaatc 
      actctaact gttgaacag gaggctgac cgaactgtc 
      acactacaa caccttcaa gatgaaaca gcagcgtat 
      cttagttta tgtgaacag accgaaatt tttgattgt 
      acaacatgt ccagaaacg tgtcaggtc gaacccagt 
      aagattttc ttaactctt ccggaaaaa gtcaaggat 
      attattttc gtaaccaat cgtaataag aaaactatt 
      tattttgag actaatgtt gtcccagaa gggctcaaa 
      ttgaagcca catgatggt actataaag ccaggagaa 
      accgtcatg attgaggtg tcctgcaaa caattgtgt 
      aaagataag actgcgttt aaagtgaga attcttgta 
      gataacgag agttttagt gttgatgtg aaagttgtt 
      tatctcaat gcctagtac ttaattttt tggaggttt 
      ttttaatac atttttaac ttttttaat aaattattt 
      tttcatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY