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Gene information
General information
TC032389
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030634
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5674600..5676393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032389
    iBeetle screen iB_06042

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032389 RA
      MDSFSYQNG GQNREQDFQ KLAQTIGTS IQKISQNVS 
      SMQRMVNQI GTHQDSPDL RKQLHSIQH YTQQLVKDT 
      NGYIKDLNA ISPSQSQSE QRQRKMQRE RLQDEFTST 
      LNMFQAAQR STAQKEKEQ VNKAKAQAY GEPFLSSYK 
      KDEQLIELQ DSSAAKEQV QLQEESELR ALQEQEQSI 
      RQLESDIND VNQIFKELG ALVHEQGEV IDSIEANVE 
      RTSDFVREG AQQLHQAST YKNKIRRKK MILGIIGAV 
      ILTIIIIII IWKTS
    CDS sequence
    • >TC032389 RA
      atggatagt ttttcgtac caaaacggg gggcagaac 
      agagagcag gatttccaa aagttggcc caaaccatt 
      ggcactagc atacagaaa atatcgcaa aatgtttcg 
      tcaatgcaa cgcatggta aaccagata ggcactcac 
      caagactca cctgatttg agaaaacag ctccattcc 
      attcagcac tacacccaa cagcttgta aaggacacc 
      aatggttac attaaggac cttaacgcc atatccccg 
      tcccaatcc cagtctgag caacggcag cgtaaaatg 
      caaagagaa cgtcttcag gatgagttt acttcaacc 
      ctgaacatg ttccaagct gctcagaga agcacagcg 
      cagaaggaa aaagaacaa gtcaataaa gccaaagcc 
      caagcttat ggcgaacca ttcctcagt agctataaa 
      aaagacgaa caattgatc gagttacaa gacagtagt 
      gcggcaaaa gagcaggtg caacttcaa gaagaatca 
      gagttacga gccctccaa gagcaggaa cagtccatt 
      agacagtta gaaagtgat attaacgat gtcaatcaa 
      attttcaaa gagttgggt gctcttgtc cacgaacaa 
      ggcgaagtt atcgacagc attgaagca aatgtggag 
      agaactagc gattttgtg agagaaggt gcccagcag 
      ttacaccag gctagcact tacaaaaat aaaattagg 
      agaaagaag atgattctt ggtattatt ggtgctgtt 
      atcttaact atcattatt ataattatt atttggaaa 
      actagttaa
    mRNA sequence
    • >TC032389 RA
      tgacatctg tcaaataat aaaagcagt gactcacaa 
      tctcaagtg ttattgatt aaaagtgca aataaaata 
      acaattatt actttccga ttaacaata cgatatttt 
      atttgatgt tttagaaga gattaataa acaataaaa 
      tatggatag tttttcgta ccaaaacgg ggggcagaa 
      cagagagca ggatttcca aaagttggc ccaaaccat 
      tggcactag catacagaa aatatcgca aaatgtttc 
      gtcaatgca acgcatggt aaaccagat aggcactca 
      ccaagactc acctgattt gagaaaaca gctccattc 
      cattcagca ctacaccca acagcttgt aaaggacac 
      caatggtta cattaagga ccttaacgc catatcccc 
      gtcccaatc ccagtctga gcaacggca gcgtaaaat 
      gcaaagaga acgtcttca ggatgagtt tacttcaac 
      cctgaacat gttccaagc tgctcagag aagcacagc 
      gcagaagga aaaagaaca agtcaataa agccaaagc 
      ccaagctta tggcgaacc attcctcag tagctataa 
      aaaagacga acaattgat cgagttaca agacagtag 
      tgcggcaaa agagcaggt gcaacttca agaagaatc 
      agagttacg agccctcca agagcagga acagtccat 
      tagacagtt agaaagtga tattaacga tgtcaatca 
      aattttcaa agagttggg tgctcttgt ccacgaaca 
      aggcgaagt tatcgacag cattgaagc aaatgtgga 
      gagaactag cgattttgt gagagaagg tgcccagca 
      gttacacca ggctagcac ttacaaaaa taaaattag 
      gagaaagaa gatgattct tggtattat tggtgctgt 
      tatcttaac tatcattat tataattat tatttggaa 
      aactagtta attgcaaat ggtattgga tctatcacg 
      aaatgtata ttatatgta tatctaaat gtactaaat 
      ccagctgta cgaagtcta tttttgctg tacattttt 
      accacagtt taaattcag tgccaaggt cattctatt 
      taaactgtt acgatatta actcattta tttattatt 
      tagattgta acgatttta ctttattgt tgcagaaac 
      tttcaagct attttataa tctccaaat ttttatcat 
      gtttttaat taaattttt gatcagtgg ccccttagt 
      atagccaac ttatctgaa acttacaag ccgctttga 
      tcataaatt taatttaat gcgttatat tttacaaac 
      tttaggcat ttttgtaca tttattgtt tgtttttac 
      tcttgttac acagtaggt tcatatttt aggtactat 
      tgtatttga agaaggctt aattgtttt attatttct 
      gattattat aaatgcatc tgatgcttt aactttagt 
      atagacaaa catagaaat tatctattc caccaaact 
      tgtaccatt attgtatta aataaaagt tgttactta 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY