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Gene information
General information
TC030601 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3929801..3930918
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030601
    iBeetle screen iB_06038

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030601 RA
      MSQATSSNS EEISNEDRL LELAKAKPE GISNENIKQ 
AIPNLPLEE LALIINKCL KNGSLELFK TKDKLLYKY 
KDSSKKPAI RGADNEEKV VFKIIEEAG NKGIWIRDI 
RFKSNLNMT QLNKVLKSL ETKKIIKAV KSVSASKKK 
VYMLYDLEA DSSVTGGAW YQDQDFESE FVDVLSQQC 
CRYLRQKWE DANSMGGGP LLARNRSYA TAQDVHKFI 
SELGISKIS LSVDDIESI LYTVVLDNN AERIVATNG 
SFLYRAVNK FMQTPGIVT TTCGVCPIA SRCADTGFI 
NPKVCNYFT EWLE
    CDS sequence
    • >TC030601 RA
      atgagccaa gctacgagt tcaaatagc gaagagata 
agcaacgaa gaccgttta ttggaatta gccaaggcc 
aagcctgag gggatttca aacgagaac ataaaacag 
gcaattcct aatttaccc ctcgaagaa ctcgccctg 
ataatcaac aagtgttta aaaaatggt tccttggag 
ttgttcaag accaaagac aagctcctc tacaaatac 
aaagactcg tcgaagaag cccgccatt cggggggcc 
gacaacgag gaaaaagtc gtgtttaaa ataatcgaa 
gaggctggc aataagggc atttggatt cgagacatt 
cgcttcaag tcgaacctc aacatgacg caactaaac 
aaagtgctc aaaagtctc gagacgaaa aaaataatc 
aaagccgta aaatcagtc tcggccagt aagaaaaaa 
gtgtacatg ttgtacgac ttggaggcc gacagttcg 
gtcacgggg ggcgcctgg taccaggac caggacttc 
gagtcggag tttgtggac gttttgagc caacagtgc 
tgccggtat ttgcgccag aagtgggag gatgcgaac 
agcatgggg ggcggcccg ctcctggcc cgaaaccgg 
agctacgcc acggcgcaa gacgtccac aaatttatt 
tccgagctg gggattagc aaaatctcg ctgtcagtg 
gacgatatt gagagcatt ttgtatact gttgttttg 
gataataac gcggagcgg attgtggcc acgaacggc 
tcgtttttg taccgagcg gtcaataaa tttatgcaa 
acacctggc attgtgaca accacttgc ggggtttgc 
cccatagcc agcaggtgc gccgatacc ggttttatt 
aacccgaaa gtttgtaat tatttcacg gaatggctc 
gaataa
    mRNA sequence
    • >TC030601 RA
      tatcaatga ccaggcgct catttcttg ccttttttc 
ctaaaaaaa atcggcaaa aatagaaat aggccaaaa 
ttttgaggt tagaaccta acctcacat aaccccaaa 
aatgagcca agctacgag ttcaaatag cgaagagat 
aagcaacga agaccgttt attggaatt agccaaggc 
caagcctga ggggatttc aaacgagaa cataaaaca 
ggcaattcc taatttacc cctcgaaga actcgccct 
gataatcaa caagtgttt aaaaaatgg ttccttgga 
gttgttcaa gaccaaaga caagctcct ctacaaata 
caaagactc gtcgaagaa gcccgccat tcggggggc 
cgacaacga ggaaaaagt cgtgtttaa aataatcga 
agaggctgg caataaggg catttggat tcgagacat 
tcgcttcaa gtcgaacct caacatgac gcaactaaa 
caaagtgct caaaagtct cgagacgaa aaaaataat 
caaagccgt aaaatcagt ctcggccag taagaaaaa 
agtgtacat gttgtacga cttggaggc cgacagttc 
ggtcacggg gggcgcctg gtaccagga ccaggactt 
cgagtcgga gtttgtgga cgttttgag ccaacagtg 
ctgccggta tttgcgcca gaagtggga ggatgcgaa 
cagcatggg gggcggccc gctcctggc ccgaaaccg 
gagctacgc cacggcgca agacgtcca caaatttat 
ttccgagct ggggattag caaaatctc gctgtcagt 
ggacgatat tgagagcat tttgtatac tgttgtttt 
ggataataa cgcggagcg gattgtggc cacgaacgg 
ctcgttttt gtaccgagc ggtcaataa atttatgca 
aacacctgg cattgtgac aaccacttg cggggtttg 
ccccatagc cagcaggtg cgccgatac cggttttat 
taacccgaa agtttgtaa ttatttcac ggaatggct 
cgaataaag tgtttcgca acaaactgg tgttttttt 
atcccgtat taattaatt aaggtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY