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Gene information
General information
TC030599 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6067559..6069118
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030599
    iBeetle screen iB_06037

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    egg chamber mostly not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030599 RA
      MEKPSKGNL KVPGTIINS QNAQLLTSS GIHSLDSIL 
      GGGLPVGTV SIIEEDIYG SHAKIMLDY FLAEGIVSK 
      HSTLVASFD TNPYDIIKN LPAVITNDP EECPIRSET 
      SSEKMKIAF RYQNLPTAE KESYSIGHN FDLSKSMSL 
      EDIENSDIC YWNGQRKET GNSMFANPA YNELLKAVK 
      EKIKEGKFF LKDNPPNRT ILRIGIHSL GSPMWLPHK 
      NSFHLMNES RDLDMFIFC LRALVRSAF AVAVITIPT 
      HLYHENSLD RCIHSSDIA MRLQTFSGT ELENNFSLS 
      DYHGFFHLT KLAAINSFA SKHPGSVEY VFKLRRKKF 
      HIEKLHLPP DVQEVHEKN ATPSMGCGS STKHLLEF
    CDS sequence
    • >TC030599 RA
      atggaaaag ccttcaaaa ggaaattta aaagttccg 
      gggactatt ataaatagt caaaatgct cagctacta 
      acttcgtct ggaattcat tctttggac tctatattg 
      ggaggcggt ttgcccgtg gggaccgtc agtattata 
      gaggaagat atttacggt tcccatgca aaaatcatg 
      ttggactat ttcctcgct gagggaatc gtcagcaaa 
      cattctaca ctagtcgcc agttttgat acaaaccct 
      tatgacata atcaaaaac cttcctgct gtgattact 
      aatgatcct gaggagtgt ccaattcgt tccgaaacc 
      tcaagtgag aaaatgaaa atagcattc agatatcag 
      aacttgcca acagcggaa aaagagtct tatagcata 
      ggtcacaat tttgatttg tccaaatct atgtcactt 
      gaagacata gaaaattcc gacatttgt tactggaat 
      ggacagagg aaggaaact ggtaacagc atgtttgcg 
      aatcctgcc tataacgaa ttactgaaa gctgtgaaa 
      gagaaaatc aaagaaggg aaattcttt ttaaaagat 
      aatccccct aataggacc atccttcga ataggcatc 
      cactctctg gggtccccc atgtggttg ccccacaag 
      aattcattc cacttaatg aatgaaagc cgcgatttg 
      gatatgttt atattttgt cttagagct ctggtgaga 
      tctgctttt gctgtggct gtgataacg ataccaaca 
      catctatat catgaaaac tctctcgac aggtgcatt 
      cactccagt gacattgca atgagacta caaacgttc 
      tctggaacg gaattagaa aataatttt agcctgtct 
      gattatcac ggtttcttt catttgact aaattagca 
      gcgatcaat tcgtttgcg tcaaaacat ccaggatca 
      gttgaatat gtgttcaag ttgaggagg aaaaagttt 
      catatagaa aaactacat ttgcctcca gatgtgcag 
      gaggttcac gaaaaaaat gctactcca tccatgggg 
      tgtggaagt agcacgaaa cacctttta gagttttaa
    mRNA sequence
    • >TC030599 RA
      agactcgaa gtcgttaga gtaggttat ttattaaat 
      tacaattaa atggaaaag ccttcaaaa ggaaattta 
      aaagttccg gggactatt ataaatagt caaaatgct 
      cagctacta acttcgtct ggaattcat tctttggac 
      tctatattg ggaggcggt ttgcccgtg gggaccgtc 
      agtattata gaggaagat atttacggt tcccatgca 
      aaaatcatg ttggactat ttcctcgct gagggaatc 
      gtcagcaaa cattctaca ctagtcgcc agttttgat 
      acaaaccct tatgacata atcaaaaac cttcctgct 
      gtgattact aatgatcct gaggagtgt ccaattcgt 
      tccgaaacc tcaagtgag aaaatgaaa atagcattc 
      agatatcag aacttgcca acagcggaa aaagagtct 
      tatagcata ggtcacaat tttgatttg tccaaatct 
      atgtcactt gaagacata gaaaattcc gacatttgt 
      tactggaat ggacagagg aaggaaact ggtaacagc 
      atgtttgcg aatcctgcc tataacgaa ttactgaaa 
      gctgtgaaa gagaaaatc aaagaaggg aaattcttt 
      ttaaaagat aatccccct aataggacc atccttcga 
      ataggcatc cactctctg gggtccccc atgtggttg 
      ccccacaag aattcattc cacttaatg aatgaaagc 
      cgcgatttg gatatgttt atattttgt cttagagct 
      ctggtgaga tctgctttt gctgtggct gtgataacg 
      ataccaaca catctatat catgaaaac tctctcgac 
      aggtgcatt cactccagt gacattgca atgagacta 
      caaacgttc tctggaacg gaattagaa aataatttt 
      agcctgtct gattatcac ggtttcttt catttgact 
      aaattagca gcgatcaat tcgtttgcg tcaaaacat 
      ccaggatca gttgaatat gtgttcaag ttgaggagg 
      aaaaagttt catatagaa aaactacat ttgcctcca 
      gatgtgcag gaggttcac gaaaaaaat gctactcca 
      tccatgggg tgtggaagt agcacgaaa cacctttta 
      gagttttaa ttcattgaa cggtacctt tgattgatt 
      accagttat ttatttcag agtattata tttttcacg 
      tttttatgt ccataatga tcagtgatg tgatataat 
      tactgtagt gataaataa agtacatgt tttcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY